FastQCFastQC Report
Wed 30 Mar 2022
TP9_S9_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP9_S9_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14618518
Sequences flagged as poor quality0
Sequence length64-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT620680.42458476296981673No Hit
TGTACAGGCTGAAGGCATGGTAACCATCGTATTCACAGCAGGCTCCAATG335680.22962655995635126No Hit
CTACGCCCAGCTTCGTCATTGGTCAAATTATTACCTGTACCATCAAAAAACACACCAATTCGAATCACTTTACAA218720.14961844969510588No Hit
AGACACATATTGAGAATAGTGATTTTTTTTCGCAATGTATTCAGCTAATT217770.14896858901839435No Hit
ATGACCGAACTCGCGGAACATGCCCCGCAAAAAACAGACTTAAAAATGAC208970.1429488269604347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)