FastQCFastQC Report
Wed 30 Mar 2022
TP8_S8_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP8_S8_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22349526
Sequences flagged as poor quality0
Sequence length64-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT635200.28421184413485995No Hit
TGTACAGGCTGAAGGCATGGTAACCATCGTATTCACAGCAGGCTCCAATG412010.18434842868703347No Hit
TTTCTATCGGGATTCTTCTTGTCTCCTTGTCGAGTTTATTTGTATTTTAA371100.16604378992198762No Hit
AGACACATATTGAGAATAGTGATTTTTTTTCGCAATGTATTCAGCTAATT320790.14353324540305687No Hit
AAACAGAGCTTTGCAAAACTTTGGCGAAATTCCTCTTTGATAGTGAAGAT279990.12527782468406715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)