FastQCFastQC Report
Wed 30 Mar 2022
TP7_S7_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP7_S7_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22230620
Sequences flagged as poor quality0
Sequence length65-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT538960.24244038177972543No Hit
TTGCAAGCATTGTGCCGTTTGCCATTACCTGAAAAACTACTTCACCTATT422110.18987774520008888No Hit
CCGAATTAACAAACTAGAACCCAAATAAATCCATTCTAAACGAATGATAC357460.16079623510275468No Hit
TGTACAGGCTGAAGGCATGGTAACCATCGTATTCACAGCAGGCTCCAATG335090.15073353779606688No Hit
ACACAACAGGAAAAATTGATGCACCTGCAGATGGTTACAAATTGGTGAAT334490.15046363979052318No Hit
TCTCACAATATGCCGAGTTCAATTGGTTGTGCACCAGTGCCTGGAAATAT332440.14954148827158217No Hit
AGACACATATTGAGAATAGTGATTTTTTTTCGCAATGTATTCAGCTAATT295410.13288428302944316No Hit
ATTTAATGTCTAACTTATCAGTGCCATCTGCTACCACGCCAATGTTACCATCGGAAAGTTTAGCTGCATCAGTTA276550.12440048905518605No Hit
CACACCGAAAGATAAAGACGGTAATCCAATTAAAGGCAAAGACGGCAAAG264040.1187731156395998No Hit
TACTTTAAACAATAAGGCTCTCCGTGATATTTATAATAAGATGACGGGTGATACCATGATAAATGGGGTGTATG260920.11736964601077253No Hit
GGTGAAACCGTTAACTTAGTTGGTGGTGTAACTGATGCAGCTAAACTTTC259760.1168478432000547No Hit
AGCTTGAGCCTTATCGTGTGGATTTTTTGTTTGGCGCAGCGAATGCCAATATTTATAACGGTAATACAGATAAAG241620.10868792683245002No Hit
ATGCACCATTCTCTAATACACGTTCTGTGGCTGAATTAGTATTGGGTGAGATTTTGCTCTTAATGCGCAATATTC240300.10809415122025386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)