FastQCFastQC Report
Wed 30 Mar 2022
TP5_S5_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP5_S5_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16692929
Sequences flagged as poor quality0
Sequence length65-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGTAAACATACCGGACAATAATTTTGCCCCATCTTCTGGTTCAAAT727280.43568147926586165No Hit
ACTTTCCAGCTCCCCCAAAATACTCTACCGCTTTCGTACGATTCATGGGG420570.25194500018540783No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT414100.24806910758441492No Hit
TTCTTCTAAGGTCTCAATCGCTTCTAAACATTTTTGACGACGCGTTAAAT362120.216930174446917No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC232810.1394662374709675No Hit
GGTGCTGTAATCGGCTTAGTGAATGGTTTAGTTATCGCTTATCTCAATGT180980.1084171627399841No Hit
AGTATTCCGTACGGCGAATTTCAAGATTTTCGTCAACTAACATGCTGATA167990.10063542473582676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)