FastQCFastQC Report
Wed 30 Mar 2022
TP4_S4_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP4_S4_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21506255
Sequences flagged as poor quality0
Sequence length64-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTCTAAGGTCTCAATCGCTTCTAAACATTTTTGACGACGCGTTAAAT562440.2615239147866516No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT559480.26014757102061703No Hit
AGTATTCCGTACGGCGAATTTCAAGATTTTCGTCAACTAACATGCTGATA230320.1070944243895555No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC221310.10290494556118675No Hit
GGTTCGCCAGACCAGCGTAAAATTCGCAGATTTTTTAAATAGAGTGCACC221150.10283054860086054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)