FastQCFastQC Report
Wed 30 Mar 2022
TP14_S14_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP14_S14_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7335557
Sequences flagged as poor quality0
Sequence length64-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACACATATTGAGAATAGTGATTTTTTTTCGCAATGTATTCAGCTAATT301740.41133890718864297No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT257440.3509481284106988No Hit
TGTACAGGCTGAAGGCATGGTAACCATCGTATTCACAGCAGGCTCCAATG210000.28627682942140587No Hit
CACATCAATCGAGCAGGACGGAAAACTTCCCATGCTTGTTTTTCTGTGGT161550.2202286752048958No Hit
GAATAGGATAATTTTATTCACTTACTTATCTAACCAACCTAGGGGAAAAA130920.1784731547992879No Hit
GACTGAGGAAAACAAAATGGCTGGTGTACGAGCAATTCAAAAAGAAAAAA88230.12027716504690782No Hit
TTACATGCGCAAGTAGGCGCAAGCAACCATGAAGAAGATAAACGTAATGC81880.1116206990144034No Hit
ATATTAACCCTGATGGCAAAAATGTGATTAAAGGCTTAGTTGATATTGAA81130.1105982817664698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)