FastQCFastQC Report
Wed 30 Mar 2022
TP13_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP13_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17743387
Sequences flagged as poor quality0
Sequence length63-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT590950.3330536610625694No Hit
AGACACATATTGAGAATAGTGATTTTTTTTCGCAATGTATTCAGCTAATT494410.2786446578660545No Hit
TGTACAGGCTGAAGGCATGGTAACCATCGTATTCACAGCAGGCTCCAATG477970.2693792340774622No Hit
AAGCAAAGGCCGTTTGTGACTTACCTGAACCACTTTCGCCTACAATACCG296240.16695797707619184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)