FastQCFastQC Report
Wed 30 Mar 2022
TP10_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP10_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15683493
Sequences flagged as poor quality0
Sequence length64-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT451790.2880672054369521No Hit
TTCTTCTAAGGTCTCAATCGCTTCTAAACATTTTTGACGACGCGTTAAAT350120.22324108538831242No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC173740.11077889345186051No Hit
GCTCGATAATGCTACAAAACTAAATAATGTAATAGCTAATTTTGAATAAA166030.10586289674117877No Hit
GGTGCTGTAATCGGCTTAGTGAATGGTTTAGTTATCGCTTATCTCAATGT157160.10020726887817658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)