FastQCFastQC Report
Fri 12 May 2023
TP3_S3_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP3_S3_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32455577
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAAGAGCACACGTCTGAACTCCAGTCACCGGAGCCTATCTCGTATG1351670.41646771524043463TruSeq Adapter, Index 7 (97% over 34bp)
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACGGAAGAGCACACGT1336720.4118614190713664No Hit
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGG897810.276627342043557No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGT814840.2510631685888684Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC704160.216961171264957No Hit
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCC645360.19884410004480896No Hit
TATCCGTCTGAAGTGGCGGTGAAATTTAATGGGGCAAGAAATACCATGGA509310.15692526433900714No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAGCCTATCTCGTATGCCGT493840.1521587491727539TruSeq Adapter, Index 7 (97% over 37bp)
AGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCACAC470970.1451121944311759No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGAGCCTATCTCGTATGCC442030.13619539101091932TruSeq Adapter, Index 7 (97% over 34bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAGCCTATCTCGTAT432720.1333268547343959TruSeq Adapter, Index 7 (97% over 35bp)
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT413290.12734021028188777No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAGCCTATCTCGTATCCCGT408400.12583353548143666TruSeq Adapter, Index 7 (97% over 37bp)
GGCCCTAAATTATATCCGTCTGAAGTGGCGGTGAAATTTAATGGGGCAAG370500.11415603549430041No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACCGGAGCCTATCTCGTATT354290.10916151637051469TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)