FastQCFastQC Report
Fri 12 May 2023
TP2_S2_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP2_S2_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8347638
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACGGAAGAGCACACGT611530.7325784850756585No Hit
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGG587810.7041632615118193No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGT585810.7017673741961499Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCC516980.6193129122273869No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTATG500820.599954142716778TruSeq Adapter, Index 16 (97% over 34bp)
TACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTATT429020.5139417880842461TruSeq Adapter, Index 16 (97% over 34bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTAT362190.4338832134311526TruSeq Adapter, Index 16 (97% over 35bp)
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTATCCCGT343570.4115775025222704TruSeq Adapter, Index 16 (97% over 37bp)
CACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAAGAG312310.3741297837783574No Hit
TATCCGTCTGAAGTGGCGGTGAAATTTAATGGGGCAAGAAATACCATGGA304010.36418685141832935No Hit
ACTGTATAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTC280070.33550808024976647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAAT265780.31838946537930846TruSeq Adapter, Index 16 (97% over 37bp)
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC260810.31243568539987No Hit
ATACACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAA223330.2675367571042252No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTTTT185880.22267376711831538TruSeq Adapter, Index 16 (97% over 34bp)
CACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGACATTTCATCGGAAGAGCACACGTCTGAA182820.21900805952534116Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCACAC168930.20236862211801707No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGGTTT162110.19419864637158438TruSeq Adapter, Index 16 (97% over 34bp)
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCT158000.18927509793788375Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAATACGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGA154480.18505833626230558No Hit
CGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCC151540.18153638190827154No Hit
GGCCCTAAATTATATCCGTCTGAAGTGGCGGTGAAATTTAATGGGGCAAG149330.17888892642445683No Hit
TTGTCCTGCATTAATACACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAAGAGCACACGT147860.17712794924743983No Hit
TGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTTATCAGATCGGAAGAGCACACGTCTGAA131920.15803272734155457Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAC127540.15278573412023858No Hit
ACTGTATAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTATC125140.14991066934143527TruSeq Adapter, Index 16 (97% over 35bp)
CTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAA120450.1442923135861905Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT119950.14369334175727314No Hit
TAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCA114500.137164548822074Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTAGCCCATCCATTCAAGACCGGAACCATTGGTGAGTGTGTCCACAAA114240.13685308347103695No Hit
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCT105370.1262273232260431TruSeq Adapter, Index 16 (97% over 37bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTTT92670.11101343877154232TruSeq Adapter, Index 16 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAATCTCGTATGCC90110.10794670300748546TruSeq Adapter, Index 16 (97% over 34bp)
TGTGTTAAAGGTAAATCACTGAGTTGTTGGCAGAATTTCCCTGTAGCCAC88430.10593415766232317No Hit
ACTTGTACATTGCCTTCAAAACCTTGATCATCATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTAGTAAT86980.10419713935846285TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)