FastQCFastQC Report
Fri 12 May 2023
TP1_S1_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP1_S1_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45957174
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGG5104891.110792843789742No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGT4601161.0011842764744412Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTAT3813900.8298813151565847TruSeq Adapter, Index 8 (97% over 36bp)
TACGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTATG3279480.7135947915335265TruSeq Adapter, Index 8 (97% over 35bp)
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTATCCCGT3016430.6563567202804942TruSeq Adapter, Index 8 (97% over 38bp)
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACGGAAGAGCACACGT2871970.6249231077611517No Hit
AGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCACAC2691050.5855560222219061No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTATT2264330.4927043599330107TruSeq Adapter, Index 8 (97% over 35bp)
ACTGTATAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTC2169740.4721221544214185Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCC1870610.4070332958244996No Hit
TATCCGTCTGAAGTGGCGGTGAAATTTAATGGGGCAAGAAATACCATGGA1576300.34299323974968526No Hit
TGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTTATCAGATCGGAAGAGCACACGTCTGAA1315140.2861664209378932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAAT1099550.23925535543155896TruSeq Adapter, Index 8 (97% over 38bp)
TACGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTTTT1072110.23328457924762735TruSeq Adapter, Index 8 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTATGCC1057380.23007942133256498TruSeq Adapter, Index 8 (97% over 35bp)
ACAATACGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGA1043700.22710273699596933No Hit
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTTT934910.20343069832796942TruSeq Adapter, Index 8 (97% over 36bp)
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCT872760.1899072384215792Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT852500.1854987863265918TruSeq Adapter, Index 8 (100% over 36bp)
GGCCCTAAATTATATCCGTCTGAAGTGGCGGTGAAATTTAATGGGGCAAG829000.18038533004662122No Hit
ATACACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAA723670.15746616621814039No Hit
CTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAA719300.15651528094395012Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TACGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGGTTT705500.153512485341244TruSeq Adapter, Index 8 (97% over 35bp)
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTATGCCGT626720.13637043913970862TruSeq Adapter, Index 8 (97% over 38bp)
TAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCA622840.13552617486880286Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATCACTTTCAGGACGGCCACGGCTTGCTTTAACAATACGCAACATTTC588430.12803876931162042No Hit
ACCTTTGTATGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTG579760.12615223033513767No Hit
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCT570000.12402851402481796TruSeq Adapter, Index 8 (97% over 38bp)
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTATCCCGG548140.11927191171502408TruSeq Adapter, Index 8 (97% over 38bp)
ACTTGTACATTGCCTTCAAAACCTTGATCATCATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAAT517810.11267228920559824TruSeq Adapter, Index 8 (97% over 36bp)
TTGTCCTGCATTAATACACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAAGAGCACACGT488990.10640123346139604No Hit
CGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCC469410.1021407452077014No Hit
GCTCGGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAAT462270.10058712487412738TruSeq Adapter, Index 8 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)