FastQCFastQC Report
Thu 4 May 2023
TP6_S6_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP6_S6_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632135
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT135180.8282403110036853No Hit
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACG45950.2815330839667062No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGGA37210.2279835920435503TruSeq Adapter, Index 3 (97% over 37bp)
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC33940.2079484846535366No Hit
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGC29530.18092866092572No Hit
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGGAAGAGCACACG28460.17437283067883477No Hit
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTC21390.13105533549614462Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACAATACGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCCTTCGT17610.10789548658658751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)