FastQCFastQC Report
Thu 4 May 2023
TP5_S5_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP5_S5_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458238
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCA185361.2711230951326191TruSeq Adapter, Index 7 (97% over 37bp)
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGGAAGAGCACACG145200.9957222346420818No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT133810.9176142714700892No Hit
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACG113330.7771708047657515No Hit
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGC109280.7493975606176769No Hit
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTC85720.5878327131785073Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACAATACGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCCTTCGT79400.5444927371252155No Hit
ACTGTATAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA73000.50060415378011TruSeq Adapter, Index 7 (97% over 35bp)
AGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCACACGTCTGAACTCC57570.39479152237151965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTTTCAGGACGGCCACGGCTTGCTTTAACAATACGCAACATTTCATCGGTATCCA56100.38471086338444066No Hit
TGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTTATCAGATCGGAAG40220.27581231595939754No Hit
CGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCCTTCGTCTTTAA38180.2618228300181452No Hit
CTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGA36280.24879340683756698No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTCGTATGCCGTCTTCTGC34530.2367926223291397TruSeq Adapter, Index 7 (97% over 34bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTCGTATGCCGTCTTCTG32170.22060870722063203TruSeq Adapter, Index 7 (97% over 35bp)
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC28830.19770435278740509No Hit
TCATCAAAAGTGGCACGATCAACCAAGGTAAAAATGCAGAAAGCAGTAAAGCAATTGGAAT23950.1642393079867621No Hit
ACATGATTAAAATAAAGAAGTGCGGTTAAAACACGCTGATTTTTAACCGCACTTCTTTATG19610.13447736240586242No Hit
CCAAAAGCCAGTCTGGGGCAACGCTATAAATTTGAATTGATTGATTGCAACGGCAACCTTC18030.1236423683925395No Hit
AGTAACCTTTGTATGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTT17670.12117363557937731No Hit
CGTTTTCATTCGCCATTAATCGCAACCACTCACGAACCATCGCAGCTCGTCCTTTTGGAGA15400.10560690367416019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)