FastQCFastQC Report
Thu 4 May 2023
TP4_S4_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP4_S4_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339076
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGC129010.9634255262584052No Hit
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGCTG117450.8770973417490866TruSeq Adapter, Index 2 (97% over 38bp)
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT102020.7618686318028253No Hit
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTC101260.7561930764198597Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACG74380.5554576439276038No Hit
AGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCACACGTCTGAACTCC55120.41162712198560797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGGAAGAGCACACG53510.3996039059769573No Hit
ACTGTATAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGAG39260.29318724254635287TruSeq Adapter, Index 2 (97% over 34bp)
TACGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGCTGATCTCGTATGCCGTCTTCTGC30090.22470718614925517TruSeq Adapter, Index 2 (97% over 35bp)
CTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGA29860.22298958386230502No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC25920.19356630990324672No Hit
ACAATACGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCCTTCGT23440.17504607654830645No Hit
CACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAAGAGCACACGTCTGA19260.14383052194199583Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGCTGATCTCGTATGCCGTCTTCTG16410.12254718925587495TruSeq Adapter, Index 2 (97% over 36bp)
TAAACGTGCAATGATACCTACAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCC15950.11911198468197474No Hit
TGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTTATCAGATCGGAAG14680.10962783292359807No Hit
CCGTAGCCCATCCATTCAAGACCGGAACCATTGGTGAGTGTGTCCACAAAGCTACCGCCAG13770.10283210213609982No Hit
TAATCACTTTCAGGACGGCCACGGCTTGCTTTAACAATACGCAACATTTCATCGGTATCCA13740.10260806705519328No Hit
ATACACCTTGTTCCTCTTCTAAAATTGACCGCACTTCGTCAAGATCGGAAGAGCACACGTC13680.1021599968933802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)