##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	TP5_S5_L001_R1_001.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1458238
Sequences flagged as poor quality	0
Sequence length	61
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.606926989970084	33.0	32.0	34.0	32.0	34.0
2	32.75367464021648	33.0	32.0	34.0	32.0	34.0
3	32.80522932470557	33.0	32.0	34.0	32.0	34.0
4	32.83457570026292	33.0	32.0	34.0	32.0	34.0
5	32.61480019036673	33.0	32.0	34.0	32.0	34.0
6	35.35967516962252	37.0	34.0	37.0	33.0	37.0
7	35.38688266250091	37.0	34.0	37.0	33.0	37.0
8	35.1855979613753	37.0	34.0	37.0	33.0	37.0
9	35.16639464888448	37.0	34.0	37.0	33.0	37.0
10	35.11960187568833	37.0	34.0	37.0	32.0	37.0
11	35.118998407667334	37.0	34.0	37.0	32.0	37.0
12	35.147651480759656	37.0	34.0	37.0	33.0	37.0
13	36.953565878820875	38.0	38.0	38.0	36.0	38.0
14	36.947204091513186	38.0	38.0	38.0	36.0	38.0
15	36.93026721289666	38.0	38.0	38.0	36.0	38.0
16	36.92085105449179	38.0	38.0	38.0	36.0	38.0
17	36.88430146519293	38.0	38.0	38.0	36.0	38.0
18	36.849296205420515	38.0	38.0	38.0	36.0	38.0
19	36.9053179247832	38.0	38.0	38.0	36.0	38.0
20	36.736222070745654	38.0	38.0	38.0	35.0	38.0
21	36.72617638547343	38.0	38.0	38.0	35.0	38.0
22	36.67252259233403	38.0	38.0	38.0	34.0	38.0
23	37.3159113944363	39.0	38.0	39.0	35.0	39.0
24	37.233253419537824	39.0	38.0	39.0	35.0	39.0
25	37.038368908230346	39.0	38.0	39.0	34.0	39.0
26	36.893458406652414	39.0	38.0	39.0	34.0	39.0
27	37.00318397956986	39.0	38.0	39.0	34.0	39.0
28	36.80200419958882	39.0	38.0	39.0	34.0	39.0
29	36.807754289766144	38.0	38.0	39.0	34.0	39.0
30	36.67051743268246	39.0	37.0	39.0	33.0	39.0
31	36.67852572762471	39.0	37.0	39.0	33.0	39.0
32	36.51051405874761	39.0	37.0	39.0	33.0	39.0
33	36.4505533390297	38.0	37.0	39.0	32.0	39.0
34	36.29240768653676	39.0	37.0	39.0	32.0	39.0
35	36.364665438700676	39.0	37.0	39.0	32.0	39.0
36	36.19402319785934	39.0	37.0	39.0	32.0	39.0
37	36.12277351159413	38.0	37.0	39.0	32.0	39.0
38	36.051630803750825	39.0	37.0	39.0	32.0	39.0
39	36.085293347176524	39.0	37.0	39.0	32.0	39.0
40	35.92662171744256	39.0	37.0	39.0	30.0	39.0
41	35.842568908504646	38.0	37.0	39.0	30.0	39.0
42	35.70291543630052	38.0	37.0	39.0	20.0	39.0
43	35.67460181397001	38.0	37.0	39.0	20.0	39.0
44	35.61418780747724	39.0	37.0	39.0	20.0	39.0
45	35.4832311323666	38.0	37.0	39.0	20.0	39.0
46	35.44881082511908	38.0	37.0	39.0	18.0	39.0
47	35.353804385840995	38.0	37.0	39.0	18.0	39.0
48	35.15729051087683	38.0	37.0	39.0	18.0	39.0
49	35.06657623789807	38.0	36.0	39.0	18.0	39.0
50	34.77798205779852	38.0	36.0	39.0	17.0	39.0
51	34.950126111101206	38.0	36.0	39.0	17.0	39.0
52	34.89284328072647	38.0	36.0	39.0	17.0	39.0
53	34.74651394354008	38.0	36.0	39.0	17.0	39.0
54	34.49568451789077	38.0	36.0	39.0	17.0	39.0
55	34.61976988667145	38.0	36.0	39.0	17.0	39.0
56	34.40675390436952	38.0	36.0	39.0	16.0	39.0
57	34.39562129090039	38.0	36.0	39.0	16.0	39.0
58	34.10346047764494	38.0	35.0	39.0	16.0	39.0
59	34.21990717564623	38.0	35.0	39.0	16.0	39.0
60	34.05625967777551	38.0	35.0	39.0	16.0	39.0
61	33.7646070120241	38.0	33.0	39.0	16.0	39.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13742692883132435
1101	2	0.23174654490825475
1101	3	0.20638189588174782
1101	4	0.2502461860279439
1101	5	0.28050308869454454
1101	6	0.2754070726584459
1101	7	0.19261532973737872
1101	8	0.1554312763635508
1101	9	0.06677009002462597
1101	10	0.21774719923529062
1101	11	0.27129749430714867
1101	12	0.13489921074212674
1101	13	0.1353293955173811
1101	14	0.24706014393576936
1101	15	0.15994898378347955
1101	16	0.09055742701540481
1101	17	0.04036193028565549
1101	18	0.07123922266978155
1101	19	0.04019965672254955
1101	20	0.1560635495774676
1101	21	0.3500025701511049
1101	22	0.15872614792517226
1101	23	0.227635232826934
1101	24	0.2704115240157847
1101	25	0.2568929469781054
1101	26	0.11216458751218994
1101	27	0.18758231041366003
1101	28	0.06273661731355418
1101	29	0.04896697091443514
1101	30	0.2510718559393794
1101	31	0.12084055628201895
1101	32	0.1872390597923399
1101	33	0.23493886639576544
1101	34	0.29684174232852456
1101	35	0.11438556376013764
1101	36	0.1717095252108365
1101	37	0.22912154146111874
1101	38	0.1361376049246914
1101	39	0.08895608543247846
1101	40	0.3118726452617011
1101	41	0.3787662085209149
1101	42	0.503798523718956
1101	43	0.26632069833413397
1101	44	0.3387636810022201
1101	45	0.39539483319229873
1101	46	0.37399463730288574
1101	47	0.14364860688716163
1101	48	0.2082162069008362
1101	49	0.2717843053929059
1101	50	0.45763508653286067
1101	51	0.35451532750195014
1101	52	0.22661551211230346
1101	53	0.4362251344277652
1101	54	0.5821128860859517
1101	55	0.4473490791822883
1101	56	0.416760044043059
1101	57	0.4411949574738827
1101	58	0.5915366804944142
1101	59	0.3841756993117116
1101	60	0.35750835721621144
1101	61	0.619837980264144
1102	1	0.19913585471764605
1102	2	0.12957718575436417
1102	3	0.2072775671469671
1102	4	0.17424308237703912
1102	5	0.14813662493868662
1102	6	0.16146262485696639
1102	7	0.1917943159890143
1102	8	0.0961667820773755
1102	9	0.1785157535394717
1102	10	0.2049601837757109
1102	11	0.2103406170143316
1102	12	0.024478553948647175
1102	13	0.15240210274343724
1102	14	0.31697789368377727
1102	15	0.09516990435226091
1102	16	0.12845958144306024
1102	17	0.12566044212707794
1102	18	0.21743221475914964
1102	19	-0.00975454165713785
1102	20	0.23446007250760914
1102	21	0.3579764746122578
1102	22	0.3253671989628941
1102	23	0.08010847653621767
1102	24	0.4313954619217455
1102	25	0.3994406380247213
1102	26	0.3838314687371138
1102	27	0.20616590187371742
1102	28	0.20997109270984993
1102	29	0.31940758600480024
1102	30	0.4675764654389667
1102	31	0.15810604191494093
1102	32	0.42260180774944445
1102	33	0.40414717269037936
1102	34	0.41019987684516934
1102	35	0.2507903204729658
1102	36	0.17543670869142858
1102	37	0.1634465718276772
1102	38	-0.06552723580739439
1102	39	0.08222136438886452
1102	40	0.24148177265764303
1102	41	-0.04543780222977034
1102	42	0.40600089857237975
1102	43	0.1467211078338977
1102	44	0.2195382538342372
1102	45	0.3356486775070735
1102	46	0.21744067064611983
1102	47	0.10299200606798564
1102	48	0.23616482851727483
1102	49	0.31355157361086583
1102	50	0.48638348500870876
1102	51	0.16695572190381824
1102	52	0.16789266796303792
1102	53	0.2390270463607962
1102	54	0.39956365372285774
1102	55	0.0699104494126388
1102	56	0.1204443596591318
1102	57	0.22360836132244089
1102	58	0.3380299665723854
1102	59	0.0742719089867947
1102	60	0.2008304722176888
1102	61	0.3286226522220801
1103	1	0.08047807834267928
1103	2	0.21401194206042362
1103	3	0.21709173900840284
1103	4	0.1487591138349984
1103	5	0.22904682773202723
1103	6	0.08642119820186878
1103	7	0.17968852677835656
1103	8	0.14824600562349133
1103	9	0.08260975916823554
1103	10	0.21587249078010018
1103	11	0.28016425782550414
1103	12	0.05921776329134332
1103	13	0.2202267430548659
1103	14	0.22316953470537015
1103	15	0.23352506887526658
1103	16	0.3161058898677922
1103	17	0.26749413054602655
1103	18	0.3013737965998118
1103	19	0.07849738501110437
1103	20	0.2782342380564131
1103	21	0.46333826740007567
1103	22	0.16500658979379068
1103	23	0.38789537256189277
1103	24	0.466854705983927
1103	25	0.46969498453567127
1103	26	0.34261523474884825
1103	27	0.17733492262649975
1103	28	0.3638645407226875
1103	29	0.31272014025399386
1103	30	0.3903617833063464
1103	31	0.27074708981204054
1103	32	0.4355591448703535
1103	33	0.36116866194575437
1103	34	0.2948792476860689
1103	35	0.2298041030553577
1103	36	0.2829454543186145
1103	37	0.25215221900408835
1103	38	0.30001039011648345
1103	39	0.33534480266972366
1103	40	0.3854788530648037
1103	41	0.1959217811364411
1103	42	0.38469576166714603
1103	43	0.30680225147260387
1103	44	0.4163082898688515
1103	45	0.6051652350177932
1103	46	0.34504463182342704
1103	47	0.3420665477445155
1103	48	0.5441720091374904
1103	49	0.428362589155725
1103	50	0.3862460537243493
1103	51	0.23570095455065854
1103	52	0.3417070765482251
1103	53	0.43978850773537204
1103	54	0.36793144351217677
1103	55	0.4147643382576902
1103	56	0.38210300402116104
1103	57	0.43335874928635576
1103	58	0.4068080775169136
1103	59	0.4418874433248945
1103	60	0.45766587030811934
1103	61	0.5951768751106314
1104	1	0.13384738390074347
1104	2	0.20126266910654067
1104	3	0.15377494108206236
1104	4	0.21100397032241602
1104	5	0.25338255143643806
1104	6	0.20950677514231586
1104	7	0.25958880630726355
1104	8	0.16152504005206225
1104	9	0.12962051583809853
1104	10	0.16975586535787812
1104	11	0.20130318008124704
1104	12	-0.1367702743163619
1104	13	0.056634593346586826
1104	14	0.16385007922226436
1104	15	0.10514300614156724
1104	16	0.26202731921335953
1104	17	0.23223634528203263
1104	18	0.30087338791860674
1104	19	-0.049979227586213426
1104	20	0.17283456590453738
1104	21	0.19645194399551258
1104	22	0.15000844127769142
1104	23	0.2777083726883376
1104	24	0.3803427097230241
1104	25	0.2636358341409917
1104	26	0.47534038196439354
1104	27	0.34252397719559724
1104	28	0.34775578410701513
1104	29	0.14449899856580117
1104	30	0.2825949777031269
1104	31	0.21145444697683047
1104	32	0.4372477772108141
1104	33	0.35701298376074675
1104	34	0.47617056738766195
1104	35	0.3720031466058771
1104	36	0.06650642232858672
1104	37	0.3585312584000562
1104	38	0.3398522914549673
1104	39	0.44177097587830616
1104	40	0.38007088310400405
1104	41	0.3332798716694896
1104	42	0.2998455850988009
1104	43	0.11683210100566299
1104	44	0.29146239203463864
1104	45	0.3251915170574975
1104	46	0.39441659493824943
1104	47	0.4467414316880891
1104	48	0.5324337141283664
1104	49	0.5205540687171535
1104	50	0.13341181572287297
1104	51	0.2990603704752246
1104	52	0.32163033028280097
1104	53	0.4714579139960975
1104	54	0.3514738402354993
1104	55	0.4243531056209946
1104	56	0.4869613429240047
1104	57	0.565859516974804
1104	58	0.25019765908155733
1104	59	0.46263902160201553
1104	60	0.4331105651293825
1104	61	0.5243785017069769
1105	1	0.011859432093508815
1105	2	0.1905322474197888
1105	3	0.15471620614230375
1105	4	0.1895431269489194
1105	5	0.13120634661716224
1105	6	0.06436370285316428
1105	7	0.24716410751208429
1105	8	0.11954461836531749
1105	9	0.29528316644050534
1105	10	0.3373010460807677
1105	11	0.3308212523704057
1105	12	0.16292852086436227
1105	13	0.11028670178031774
1105	14	0.16046152500540245
1105	15	0.11474390975602944
1105	16	0.16959509029769748
1105	17	0.29360682720974296
1105	18	0.17568513490655846
1105	19	-0.41500181794765467
1105	20	0.09678034903706845
1105	21	0.017235076525629722
1105	22	0.05098735694033962
1105	23	-0.08392566345624175
1105	24	0.07800837237362401
1105	25	-0.08369097308792561
1105	26	0.165852430157166
1105	27	0.10381915791849394
1105	28	0.27207807326364275
1105	29	0.11324899856580117
1105	30	0.13538112228143717
1105	31	0.14330685661538922
1105	32	0.20306103022286237
1105	33	0.15332322472460191
1105	34	0.18249586859248268
1105	35	0.21368236347334602
1105	36	0.34305009702737976
1105	37	0.4155719210506561
1105	38	0.19019114687665706
1105	39	0.012177602384326747
1105	40	0.2738961843088248
1105	41	0.2662617993803309
1105	42	-0.11506405345541282
1105	43	-0.1990564532111989
1105	44	0.07007684986596274
1105	45	-0.2329786636653992
1105	46	-0.04157738096536434
1105	47	-0.006007062287814335
1105	48	0.31387196714041465
1105	49	0.029966719319560298
1105	50	-0.6529210156024305
1105	51	-0.30052547289827203
1105	52	0.00894207727075269
1105	53	0.10003472122500767
1105	54	-0.2774794730175145
1105	55	-0.102567075101895
1105	56	0.1234447766589426
1105	57	0.12346493866154873
1105	58	-0.4732586662196496
1105	59	-0.1811862796027981
1105	60	0.10291478199685145
1105	61	0.19305320050215613
1106	1	0.14918770170186946
1106	2	0.24794799906015186
1106	3	0.2083612294043391
1106	4	0.1880278921293268
1106	5	0.24157638813060345
1106	6	0.29886531487142776
1106	7	0.269509959682523
1106	8	0.23328741416273147
1106	9	0.2606760703859834
1106	10	0.22497287566481816
1106	11	0.22377217253582415
1106	12	0.1737270310511434
1106	13	0.13698724218053826
1106	14	0.21273868501918258
1106	15	0.17362636278983956
1106	16	0.03632733259016163
1106	17	0.3191315833664774
1106	18	0.23778014649223422
1106	19	0.2464633643849865
1106	20	0.07144221630289849
1106	21	-0.3609105980053897
1106	22	-0.04281507333023171
1106	23	0.07236961079091486
1106	24	0.1827142442238383
1106	25	-0.4737789879248382
1106	26	0.12969332724193094
1106	27	0.15891387816190417
1106	28	0.1459610959452604
1106	29	0.17424162828679357
1106	30	0.2112903056842228
1106	31	0.3134865875829149
1106	32	0.450059680639356
1106	33	0.2571537236113812
1106	34	0.08935632698608487
1106	35	0.2183559552812966
1106	36	0.28056235437441757
1106	37	0.3878933435490808
1106	38	0.11124865976599807
1106	39	0.20389476743142154
1106	40	0.1968576245371807
1106	41	-0.25938326299185377
1106	42	-0.7441927955596981
1106	43	-0.030064763834197095
1106	44	0.3029756543424327
1106	45	-0.6045547544417502
1106	46	-0.027055754754080397
1106	47	0.023965977216832357
1106	48	0.30654667897231036
1106	49	-0.34812510790654017
1106	50	-1.1055462275907644
1106	51	-0.1816523042734488
1106	52	-0.0538694577288652
1106	53	-0.4591810891372532
1106	54	-0.5726288794501642
1106	55	-0.10276340520968574
1106	56	0.08259670711316147
1106	57	0.16805773400551516
1106	58	-0.4844078924055353
1106	59	-0.021439676517523765
1106	60	0.07500033912941007
1106	61	-0.2149142451177326
1107	1	0.07090301761249407
1107	2	0.18182314193791171
1107	3	0.21841051892266705
1107	4	0.24905643926554433
1107	5	0.29299288024241577
1107	6	0.21064646528705566
1107	7	0.11380168855423989
1107	8	0.18241359984266836
1107	9	0.18416233225253364
1107	10	0.2390502523804301
1107	11	0.3149986633466568
1107	12	0.30812301088960226
1107	13	0.25717546891399223
1107	14	0.18744859058823238
1107	15	0.22604338225742993
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2109	27	-0.1744697852557806
2109	28	-0.32486451994383003
2109	29	-0.006499187090213354
2109	30	0.05245937624216168
2109	31	-0.35568339573259067
2109	32	-0.2396350493711097
2109	33	0.18233671964918585
2109	34	0.34203303299813825
2109	35	0.1296441436649758
2109	36	-0.24790575393552672
2109	37	0.09622929463949959
2109	38	0.15240446866776125
2109	39	-0.35653533993613706
2109	40	-0.3797618533536351
2109	41	-0.08605475028289078
2109	42	-0.007966470209041177
2109	43	-0.24325588874862802
2109	44	-0.287090994740808
2109	45	0.014151293169817336
2109	46	-0.0330102383792692
2109	47	-0.1812237755977506
2109	48	-0.20571790810861046
2109	49	0.04450933861564721
2109	50	4.03376847373238E-5
2109	51	-0.05176053124188229
2109	52	-0.2358339757602721
2109	53	0.037642336726385395
2109	54	0.05838228108551391
2109	55	0.012004300953506686
2109	56	-0.302395691512956
2109	57	0.022270199073275876
2109	58	0.28828672795548016
2109	59	-0.14253274815417427
2109	60	-0.15434259396368333
2109	61	0.20898325932010664
2110	1	-0.0776808637474673
2110	2	-0.09480546247348798
2110	3	-0.1344737564431142
2110	4	-0.2137566112331939
2110	5	-0.2197059495028313
2110	6	-0.16161898613798087
2110	7	-0.1996311715278125
2110	8	-0.15666347814530468
2110	9	-0.15696889430797967
2110	10	-0.17314964568610236
2110	11	-0.008346295741787912
2110	12	-0.22069443672763356
2110	13	-0.17616383935407498
2110	14	-0.24976516484443323
2110	15	-0.16236835744012978
2110	16	-0.33230010527797305
2110	17	-0.3652554135280113
2110	18	-0.40844205061733163
2110	19	-0.3018925757282318
2110	20	-0.31517895253335837
2110	21	0.024445366076726316
2110	22	-0.40297224769901874
2110	23	-0.16743389532562247
2110	24	-0.47363086825829726
2110	25	-0.07709321050635509
2110	26	-0.6383709391636003
2110	27	-0.23570200819936105
2110	28	-0.5707805718378225
2110	29	-0.2342050645422944
2110	30	-0.40340550005137743
2110	31	-0.18246000099272663
2110	32	-0.29273663187692733
2110	33	-0.03402426365174449
2110	34	0.2327941118750516
2110	35	-0.0862923281660457
2110	36	-0.4557980831759636
2110	37	-0.09925737266643608
2110	38	0.17517957583705623
2110	39	0.07477896360882141
2110	40	-0.3152412974408918
2110	41	-0.16095001754197824
2110	42	-0.10581958656810286
2110	43	-0.06725536685339506
2110	44	-0.32951856212739017
2110	45	0.044149501119918
2110	46	0.06823692617335553
2110	47	0.048210368926362435
2110	48	-0.4067554892813945
2110	49	-0.14366765146363036
2110	50	-0.03624462497303682
2110	51	-0.2024701201236283
2110	52	-0.2724024438562793
2110	53	-0.00768505035482292
2110	54	0.12803615607903396
2110	55	-0.06063062547026732
2110	56	-0.23059870561513662
2110	57	-0.25153333252261234
2110	58	0.2917480566189994
2110	59	-0.03601907905775903
2110	60	0.0029164657131488525
2110	61	0.02870091714232359
2111	1	-0.1472070721107599
2111	2	-0.2867993776863642
2111	3	-0.3846242987898947
2111	4	-0.23380217136283932
2111	5	-0.2693501298697285
2111	6	-0.04320328080572011
2111	7	-0.00965105981874359
2111	8	3.033232079232562E-4
2111	9	-0.17319940054315452
2111	10	-0.24451916660576245
2111	11	-0.014927787553816074
2111	12	-0.16963595469861303
2111	13	0.033095821683637894
2111	14	-0.04291982572672737
2111	15	-0.0013870548459919974
2111	16	-0.13062180166986082
2111	17	-0.15241428279183822
2111	18	0.001596448635524439
2111	19	0.0114268343065973
2111	20	-0.10250974316753059
2111	21	0.10621502809021877
2111	22	0.03113694444605386
2111	23	0.16608818939901937
2111	24	-0.10462490084317722
2111	25	0.130029128075833
2111	26	-0.15907766396058776
2111	27	0.004627173093055603
2111	28	-0.30775622325628404
2111	29	-0.057838867616204936
2111	30	-0.07496427752071355
2111	31	-0.0750890935679962
2111	32	-0.012229693088663396
2111	33	0.035799975258882455
2111	34	-0.2040609123655912
2111	35	-0.053712010692571255
2111	36	-0.18300916519579857
2111	37	-0.12253553394360495
2111	38	-0.2613913741446865
2111	39	0.12892990399977577
2111	40	-0.050593534831101294
2111	41	-0.03223515124167875
2111	42	-0.09336187876128577
2111	43	-0.14359866154881473
2111	44	-0.1604022897754831
2111	45	0.15685395301355953
2111	46	-0.18805805579481216
2111	47	0.17186316183097006
2111	48	-0.14322194880220707
2111	49	0.0809577671670354
2111	50	0.005585541610152234
2111	51	0.040255058211450034
2111	52	-0.16809960028377446
2111	53	-0.0564741084391116
2111	54	-0.13333307720964882
2111	55	-0.04849967264932786
2111	56	-0.12482565790658384
2111	57	0.026739053711992256
2111	58	0.10638446089765097
2111	59	0.0023700312331058626
2111	60	0.062435280722972664
2111	61	0.0035656803207331222
2112	1	-0.06268389587773271
2112	2	-0.1363951273101378
2112	3	-0.18281407355125623
2112	4	-0.1778610040622013
2112	5	-0.09679930362672451
2112	6	-0.13932458200121545
2112	7	-0.04396305776609921
2112	8	0.04499878428506321
2112	9	-0.19453377556400397
2112	10	-0.3165725729057485
2112	11	-0.021058417837444665
2112	12	-0.21003445476564053
2112	13	-0.14860924811165432
2112	14	-0.13274217271421662
2112	15	-0.03363867697303391
2112	16	-0.2732678355639351
2112	17	-0.20759374642386774
2112	18	-0.07064120412222508
2112	19	-4.8534367153507674E-4
2112	20	-0.2980948644244492
2112	21	-0.027098581520810683
2112	22	-0.052273474167961353
2112	23	0.1419132344218781
2112	24	-0.20791846929217428
2112	25	-0.1475855490424891
2112	26	-0.13617454029315468
2112	27	0.10697479141567356
2112	28	-0.2668935434302284
2112	29	-0.08031268945488534
2112	30	0.07549410946902668
2112	31	-0.21068280996007616
2112	32	-0.06361565105196831
2112	33	-0.18752204751549328
2112	34	-0.18472428453372913
2112	35	-0.17868600388803912
2112	36	-0.13363232765961897
2112	37	-0.22116182874885482
2112	38	-0.2414771374588085
2112	39	0.08929750525344105
2112	40	-0.12566206238193445
2112	41	0.0049739895662881395
2112	42	-0.13143174623121467
2112	43	-0.06401874953824915
2112	44	-0.04007843454498072
2112	45	-0.14103556826107422
2112	46	-0.04923752427941963
2112	47	0.01841998222539587
2112	48	-0.1288627049072062
2112	49	0.004977864133742571
2112	50	0.09972941580212336
2112	51	0.04920321868875277
2112	52	-0.04966976831047276
2112	53	-0.031205047953505982
2112	54	-0.04877108032378885
2112	55	-0.005382968668335764
2112	56	0.02610676564857073
2112	57	-0.10882630540634608
2112	58	0.11800851684306224
2112	59	-0.06551121638988633
2112	60	0.08674595634184357
2112	61	-0.17210368781854868
2113	1	-0.0941960722328119
2113	2	-0.13873733666035548
2113	3	-0.353439946613463
2113	4	-0.1793421643599089
2113	5	-0.29987441670031956
2113	6	-0.1318453147220211
2113	7	-0.014599380962202702
2113	8	-0.22443396685437023
2113	9	-0.25647945555811447
2113	10	-0.3767369698543135
2113	11	-0.06680136763286981
2113	12	-0.04012644742925886
2113	13	-0.20274352798423223
2113	14	-0.1660054211825681
2113	15	-0.18098848853328064
2113	16	-0.17365084721455304
2113	17	-0.2541465066310735
2113	18	-0.26674137757645866
2113	19	-0.23496277175714653
2113	20	-0.007128465428145603
2113	21	-0.021776584708625535
2113	22	-0.0709268507231684
2113	23	-0.047186698694268614
2113	24	-0.1099318217745946
2113	25	-0.03988796092439628
2113	26	-0.21847620402343892
2113	27	-0.12898072339885402
2113	28	-0.3305908952583465
2113	29	-0.18430224171986964
2113	30	-0.18397027094223262
2113	31	-0.3594523854072662
2113	32	-0.5006555809086279
2113	33	-0.1757232166410887
2113	34	-0.5195935776473917
2113	35	-0.26710995427553996
2113	36	-0.3281802364259079
2113	37	-0.179634615895516
2113	38	-0.5426698995491392
2113	39	-0.060851915168939286
2113	40	-0.23002123003551844
2113	41	-0.26803557103181674
2113	42	-0.22573588350969231
2113	43	-0.0938433117308648
2113	44	-0.31988657938233445
2113	45	-0.7055589979260475
2113	46	-0.38620164193802964
2113	47	-0.12467728453233917
2113	48	-0.4932823753876505
2113	49	-0.3922027886952648
2113	50	-0.29313894504564786
2113	51	-0.12721412007745414
2113	52	-0.1723269030157013
2113	53	-0.4823048683221529
2113	54	-0.35428100712637445
2113	55	-0.1961097192569241
2113	56	-0.3229527822729352
2113	57	-0.26213569189052777
2113	58	-0.16567854677575866
2113	59	-0.28041248511410544
2113	60	-0.2445843472023128
2113	61	-0.3087109641403316
2114	1	-0.15559215453386344
2114	2	-0.22529097084824912
2114	3	-0.1305272031296596
2114	4	-0.1395370175024908
2114	5	-0.3236097360282244
2114	6	-0.12124091573823392
2114	7	-0.2983775162679976
2114	8	-0.16214107702008818
2114	9	-0.27630937693369617
2114	10	-0.3813744898840383
2114	11	-0.22224292799059953
2114	12	-0.1315816585308056
2114	13	-0.42911589838041664
2114	14	-0.2307178428463459
2114	15	-0.18287172833759513
2114	16	-0.2056536004865066
2114	17	-0.37004955372652404
2114	18	-0.23303194523377613
2114	19	-0.21135505564978274
2114	20	-0.07713569254293873
2114	21	-0.2582446216703147
2114	22	-0.28437141283742307
2114	23	-0.4909201202183411
2114	24	-0.3435145023317503
2114	25	-0.608498218453235
2114	26	-0.5002629896848703
2114	27	-0.32294781341916945
2114	28	-0.5482516291034614
2114	29	-0.4926847202180582
2114	30	-0.49603677429021786
2114	31	-0.41335315088095115
2114	32	-0.3309650316019699
2114	33	-0.4602641704970054
2114	34	-0.487822840415582
2114	35	-0.35122512340806367
2114	36	-0.430104289095226
2114	37	-0.627481252732828
2114	38	-1.0149415180721988
2114	39	-0.47214342220554784
2114	40	-0.4634904232683539
2114	41	-0.4223080887150559
2114	42	-0.5039683761293716
2114	43	-0.2855631734802202
2114	44	-0.4024690534665041
2114	45	-0.8088769393309647
2114	46	-0.6111176103439675
2114	47	-0.5564454674591133
2114	48	-0.46870872866797697
2114	49	-0.31006698189841586
2114	50	-0.42683171839017575
2114	51	-0.36898883776792957
2114	52	-0.29597386355516164
2114	53	-0.43015144914399883
2114	54	-0.8329280813772257
2114	55	-0.3772881434008397
2114	56	-0.7315065896606399
2114	57	-0.609003480950193
2114	58	-0.29647957795318547
2114	59	-0.24167857793597847
2114	60	-0.22647768547396652
2114	61	-0.7499235778811268
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	21.0
15	96.0
16	80.0
17	60.0
18	62.0
19	133.0
20	362.0
21	1332.0
22	3285.0
23	6381.0
24	10042.0
25	14506.0
26	19860.0
27	24522.0
28	28367.0
29	33388.0
30	37169.0
31	39108.0
32	44552.0
33	53518.0
34	68488.0
35	96295.0
36	177722.0
37	684881.0
38	114003.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.731725548230123	31.353935365831916	32.867200004388856	20.047139081549105
2	21.93578825952965	29.819275042894233	30.39538127521022	17.849555422365896
3	18.47537919050251	27.551126770801478	37.45815155002133	16.51534248867469
4	21.012207883761086	29.04957901247944	28.51160098694452	21.42661211681495
5	21.048278813197847	27.701788048315844	27.535354311161825	23.714578827324484
6	20.607747157871348	28.047204914424118	28.725420678928952	22.61962724877558
7	23.152187777303844	27.070409631349612	25.365749623861127	24.411652967485416
8	19.956687454311297	27.758774630753003	28.38850722584379	23.896030689091905
9	19.471101425144592	32.05718133802576	27.874805072971625	20.596912163858025
10	19.933714523966593	30.421645849305808	28.8066145581174	20.8380250686102
11	17.445094696476158	29.831412979225615	30.174566840255157	22.54892548404307
12	19.918559247530236	32.54633331458925	27.66880303489554	19.866304402984973
13	19.841274195295966	33.11551337984609	27.03975619891952	20.003456225938425
14	18.31594019631912	28.750999493909774	30.575255891013676	22.357804418757432
15	21.379980497010777	28.901592195512666	28.66685685052783	21.051570456948728
16	23.037528853314754	24.969380855525642	29.057259514564837	22.935830776594766
17	21.278350996202267	26.798026110964052	29.341575243547354	22.58204764928633
18	21.827164015750515	30.68724035445517	28.357922369325173	19.12767326046914
19	19.966425233740996	28.937457397214995	29.14208791706155	21.95402945198246
20	18.225694296815746	28.066406169637602	31.386851803340743	22.321047730205905
21	19.99913594351539	30.252469075692716	31.61616965131892	18.132225329472966
22	18.861667299850915	30.025002777324417	29.81934361880571	21.29398630401896
23	17.887957932792865	29.186867987255855	32.13933527997487	20.78583879997641
24	18.89348652277612	29.16814676342271	29.415637227942216	22.52272948585896
25	22.232173348932065	25.736196697658407	28.5923834106641	23.439246542745423
26	19.527333672555507	28.369168818807356	28.392073173240583	23.711424335396554
27	19.98953531590865	28.446796750599013	28.11276348579587	23.450904447696466
28	19.53165395497854	29.33252322323242	27.051688407516465	24.08413441427257
29	19.53062531630639	28.499325898790183	28.48999957482935	23.480049210074075
30	20.18319368991893	30.117031650526183	28.06517180323102	21.634602856323866
31	20.79633091443235	29.421603332240693	26.907061810212053	22.875003943114912
32	17.92972066288219	29.65928744141903	28.317188277907995	24.093803617790783
33	20.48533915588539	32.656191924774966	26.935040782094553	19.923428137245086
34	18.240300965960287	30.102630709116067	26.621374563000007	25.03569376192364
35	18.25915934161639	31.027308299468263	27.56209891663775	23.151433442277597
36	19.688692792260248	29.680203094419426	26.598538784478254	24.032565328842068
37	21.00192149703958	28.581685568473738	28.040621626922352	22.375771307564335
38	18.30229358993525	28.632431742966514	28.725352103017478	24.33992256408076
39	20.35627929048619	27.724623826837597	25.066827225734073	26.852269656942145
40	19.11155792127211	26.95273336725555	28.27748282516297	25.658225886309367
41	20.272410950750153	29.782381202519755	24.957380071017212	24.98782777571288
42	18.388150631104114	31.415036502957676	27.420969690818648	22.77584317511956
43	18.104794964882277	30.28387684314906	27.396213786775547	24.215114405193116
44	16.802949861408084	29.5332449161248	26.223702852346463	27.440102370120652
45	18.710388839133255	30.339971938737026	25.836934711617722	25.112704510512003
46	19.548180749644434	29.604220984503215	24.176711894766147	26.6708863710862
47	18.635983975181006	27.16278138410877	27.694999033079647	26.50623560763058
48	18.200184057746405	30.506268524068087	25.511610587572125	25.781936830613382
49	21.732460682001154	27.03763034566374	24.668606907788714	26.56130206454639
50	17.86498500244816	26.72307263971999	26.179608541266923	29.23233381656492
51	21.307290030845444	28.131484709629017	23.27836745442102	27.282857805104516
52	17.349294148143173	31.311006845247487	24.16203664971013	27.17766235689922
53	18.713749058795614	27.825430416708386	24.957585798751644	28.50323472574436
54	17.043034127488106	31.83787557312318	25.72522455182213	25.393865747566586
55	18.07386722880627	28.24264626213279	25.434256959426378	28.249229549634556
56	17.844755108562527	28.451871368048288	24.511430918684056	29.19194260470513
57	17.644033415670144	31.36099868471402	23.16521720048442	27.829750699131417
58	18.200595513215266	27.448674359055243	24.783402983600755	29.567327144128736
59	18.270817246567432	29.60977563333283	23.02374509510793	29.095662024991803
60	18.928803117186632	28.24319486942461	22.917589584141957	29.9104124292468
61	19.09791131488824	27.94200946621882	22.83536706628136	30.124712152611576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	13.0
2	2.5
3	2.0
4	2.5
5	3.0
6	2.5
7	2.0
8	2.0
9	2.5
10	3.0
11	4.5
12	23.0
13	40.0
14	78.5
15	117.0
16	139.0
17	329.0
18	497.0
19	681.5
20	1310.0
21	1754.0
22	2716.0
23	3678.0
24	4991.5
25	8446.5
26	10588.0
27	14003.0
28	17418.0
29	21810.0
30	31744.0
31	37286.0
32	45076.5
33	52867.0
34	60411.0
35	74496.0
36	81037.0
37	91058.5
38	101080.0
39	105355.0
40	120418.0
41	131206.0
42	130691.5
43	130868.5
44	131560.0
45	119042.5
46	106525.0
47	95447.0
48	78139.5
49	71910.0
50	73930.5
51	75951.0
52	61187.0
53	44935.5
54	43448.0
55	36227.5
56	29007.0
57	24845.0
58	18333.5
59	15984.0
60	13930.5
61	11183.0
62	10489.0
63	9662.5
64	8836.0
65	7592.5
66	5408.0
67	4467.0
68	3935.5
69	3404.0
70	2920.5
71	2080.0
72	1723.0
73	1520.5
74	1318.0
75	1101.0
76	745.5
77	607.0
78	491.5
79	376.0
80	317.5
81	212.0
82	165.0
83	139.5
84	92.5
85	71.0
86	56.0
87	41.0
88	31.5
89	17.5
90	13.0
91	11.0
92	9.0
93	7.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1458238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.77102671213519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.95936344952604	23.03866698378519
2	4.196995847379538	2.3310976757659527
3	2.1594287478038785	1.7990866031464245
4	1.4171224670905564	1.5741978355175503
5	0.998038127421224	1.3858271748172097
6	0.8128800795043667	1.3544708641007004
7	0.6339443823377094	1.2323700463136003
8	0.5038375763889453	1.1193669433979885
9	0.43403599712782354	1.084826274323852
>10	4.968122153194562	30.294791685648665
>50	0.672931794923666	12.72703877494796
>100	0.2263828561805708	10.279608599101081
>500	0.009702122407738748	1.8509692901353971
>1k	0.004726675019154775	2.7511682245361224
>5k	0.0012438618471459933	2.4303989495996245
>10k+	0.0012438618471459933	4.746114074862702
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTTGGTCGCCACAAGGTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCA	18536	1.2711230951326191	TruSeq Adapter, Index 7 (97% over 37bp)
AAGAACGGAGTAACCTGAATTCAACACAAGCGAATTGACTAAACCATCGGAAGAGCACACG	14520	0.9957222346420818	No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT	13381	0.9176142714700892	No Hit
TGAAAAACAAGGATTGATCGAACCCGCCAAGCGAACAATTGGTAATTTTCGCGTTTATACG	11333	0.7771708047657515	No Hit
CATTCTGCTACTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGC	10928	0.7493975606176769	No Hit
AACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGAGCACACGTCTGAACTCCAGTC	8572	0.5878327131785073	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACAATACGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCCTTCGT	7940	0.5444927371252155	No Hit
ACTGTATAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA	7300	0.50060415378011	TruSeq Adapter, Index 7 (97% over 35bp)
AGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCACACGTCTGAACTCC	5757	0.39479152237151965	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCACTTTCAGGACGGCCACGGCTTGCTTTAACAATACGCAACATTTCATCGGTATCCA	5610	0.38471086338444066	No Hit
TGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTTATCAGATCGGAAG	4022	0.27581231595939754	No Hit
CGCAACATTTCATCGGTATCCATTTTTCCATAGGCTTCAGAAGATGCCCCTTCGTCTTTAA	3818	0.2618228300181452	No Hit
CTAAATCGGCTTTTGCCATTAAACATGCCCCAAACATGCCGTTTTGCACGCGATCGGAAGA	3628	0.24879340683756698	No Hit
TACGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTCGTATGCCGTCTTCTGC	3453	0.2367926223291397	TruSeq Adapter, Index 7 (97% over 34bp)
TTTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTCGTATGCCGTCTTCTG	3217	0.22060870722063203	TruSeq Adapter, Index 7 (97% over 35bp)
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC	2883	0.19770435278740509	No Hit
TCATCAAAAGTGGCACGATCAACCAAGGTAAAAATGCAGAAAGCAGTAAAGCAATTGGAAT	2395	0.1642393079867621	No Hit
ACATGATTAAAATAAAGAAGTGCGGTTAAAACACGCTGATTTTTAACCGCACTTCTTTATG	1961	0.13447736240586242	No Hit
CCAAAAGCCAGTCTGGGGCAACGCTATAAATTTGAATTGATTGATTGCAACGGCAACCTTC	1803	0.1236423683925395	No Hit
AGTAACCTTTGTATGGAATACGGCCTTCGATACCTTCTGGTACGAGTTTGTCTGCAGCGTT	1767	0.12117363557937731	No Hit
CGTTTTCATTCGCCATTAATCGCAACCACTCACGAACCATCGCAGCTCGTCCTTTTGGAGA	1540	0.10560690367416019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018515496098716396	0.0	0.0	0.0	0.0
2	0.002468732813162186	0.0	0.0	0.0	0.0
3	0.0030859160164527326	0.0	0.0	0.0	0.0
4	0.00473173789189419	0.0	0.0	0.0	0.0
5	0.005828952475521829	0.0	0.0	0.0	0.0
6	0.006446135678812375	0.0	0.0	0.0	0.0
7	0.006789015236196012	0.0	0.0	0.0	0.0
8	0.008023381642777105	0.0	0.0	0.0	0.0
9	0.010560690367416019	0.0	0.0	0.0	0.0
10	0.013646606383868751	0.0	0.0	0.0	0.0
11	0.016389642842937847	0.0	0.0	0.0	0.0
12	0.021121380734832038	0.0	0.0	0.0	0.0
13	0.027704668236597868	0.0	0.0	0.0	0.0
14	0.0322992543055386	0.0	0.0	0.0	0.0
15	0.03997975639093207	0.0	0.0	0.0	0.0
16	0.05115762996163863	0.0	0.0	0.0	0.0
17	0.0600724984536132	0.0	0.0	0.0	0.0
18	0.08242824559502633	0.0	0.0	0.0	0.0
19	0.0916174177329078	0.0	0.0	0.0	0.0
20	1.416298299728851	0.0	0.0	0.0	0.0
21	1.4282305083258013	0.0	0.0	0.0	0.0
22	1.4395455337194614	0.0	0.0	0.0	0.0
23	1.454015051041051	0.0	0.0	0.0	0.0
24	1.4613526735690607	0.0	0.0	0.0	0.0
25	1.4904288600351931	0.0	0.0	0.0	0.0
26	1.4996180321730745	0.0	0.0	0.0	0.0
27	1.5119616962388855	0.0	0.0	0.0	0.0
28	1.530751495983509	0.0	0.0	0.0	0.0
29	1.5522843321872013	0.0	0.0	0.0	0.0
30	1.5683310954727554	0.0	0.0	0.0	0.0
31	1.5789603617516481	0.0	0.0	0.0	0.0
32	1.5900010834994014	0.0	0.0	0.0	0.0
33	1.6100252496506058	0.0	0.0	0.0	0.0
34	2.028269733747166	0.0	0.0	0.0	0.0
35	2.04747098896065	0.0	0.0	0.0	0.0
36	2.0586488625313564	0.0	0.0	0.0	0.0
37	2.0717468616234114	0.0	0.0	0.0	0.0
38	2.087656473086012	0.0	0.0	0.0	0.0
39	2.172416299671247	0.0	0.0	0.0	0.0
40	2.183525597330477	0.0	0.0	0.0	0.0
41	2.2042355225964485	0.0	0.0	0.0	0.0
42	2.2759659260011054	0.0	0.0	0.0	0.0
43	2.2900239878538344	0.0	0.0	0.0	0.0
44	2.3058650234049587	0.0	0.0	0.0	0.0
45	2.321637483044606	0.0	0.0	0.0	0.0
46	2.3355583930743817	0.0	0.0	0.0	0.0
47	2.3479020571401925	0.0	0.0	0.0	0.0
48	2.359834265737143	0.0	0.0	0.0	0.0
49	2.374920966262023	0.0	0.0	0.0	0.0
50	2.3895962113180427	0.0	0.0	0.0	0.0
>>END_MODULE
