FastQCFastQC Report
Thu 11 May 2023
Tp12_S12_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp12_S12_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11872819
Sequences flagged as poor quality0
Sequence length63-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC510500.429973707170976No Hit
CTCATCCACAGCAAGCGGCATTGTGTCTATTGCTCATGGTAACGCCGCTAAAGCTACAGGTGATGGCTCTATTG232840.19611180798763966No Hit
CAACAGCATTGGCTTAATCCCATTCAAGGTTGCTATCGTTTTCATTTTGA202480.17054079574530698No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACCATTGGTGAGTGTGT152790.1286888985673916No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG120470.10146705681270808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)