FastQCFastQC Report
Thu 11 May 2023
Tp5_S5_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp5_S5_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17417214
Sequences flagged as poor quality0
Sequence length61-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAGCATTGGCTTAATCCCATTCAAGGTTGCTATCGTTTTCATTTTGA462500.2655418943580759No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG392110.22512785339836783No Hit
CTCATCCACAGCAAGCGGCATTGTGTCTATTGCTCATGGTAACGCCGCTAAAGCTACAGGTGATGGCTCTATTG299830.17214578634677163No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT297420.1707620977729274No Hit
TAATCACCTGCTACACGTACATTACCTAATTTGTAACCTACTGCAACACGTGGCTCAATTTTAGTTTTGCTTGAA230850.132541289324458No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC203160.11664322434116042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)