FastQCFastQC Report
Thu 11 May 2023
Tp4_S4_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp4_S4_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12211771
Sequences flagged as poor quality0
Sequence length46-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATCCACAGCAAGCGGCATTGTGTCTATTGCTCATGGTAACGCCGCTAAAGCTACAGGTGATGGCTCTATTG295680.24212704283432762No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG260610.2134088495436084No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC191900.15714346428540135No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT176890.14485204480169175No Hit
GACATTGCTTGGTTCTCCTTGGGTATTTAATGGGGTATGACATTTAACTTGTCGATTTCCTACTTGGCGTAATTC148350.12148115125971491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)