FastQCFastQC Report
Fri 20 Oct 2023
TP1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428588
Sequences flagged as poor quality0
Sequence length30-61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT194151.3590342352028717No Hit
TCATGCGCGTATTAAAATTTGGTGGCACTTCATTAGCCAACCCTGAGCGTTTCTCGCAAGC32230.22560738295435775No Hit
TTTATTGGTGGCATTTTTTATGCCTTTTTTAATCCCTATTATCGTCGGATTATCTTTGCAT29470.2062876070637581No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACC28670.20068767202300453No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC28280.19795770369063717No Hit
TCATCAAAAGTGGCACGATCAACCAAGGTAAAAATGCAGAAAGCAGTAAAGCAATTGGAAT24960.17471797327151006No Hit
GGTAAGCTATTTAAGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCAC22300.15609818926100458No Hit
TGCGGCAAGATGCCCTGTGAGTTTAATTTCAATCCCTTTCCCTGCCTTTAACCAATCAAAA15660.1096187284227503No Hit
CTTGGAAGCATGACGCCGATTTCTTGCAAGTTAGGTGCGATGTTATCAGCAATATTGGGTA14560.1019188177417142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)