FastQCFastQC Report
Fri 20 Oct 2023
TP1_trimmed.trim.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP1_trimmed.trim.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423654
Sequences flagged as poor quality0
Sequence length20-61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT194151.3637442805625524No Hit
TCATGCGCGTATTAAAATTTGGTGGCACTTCATTAGCCAACCCTGAGCGTTTCTCGCAAGC32230.22638927716987414No Hit
TTTATTGGTGGCATTTTTTATGCCTTTTTTAATCCCTATTATCGTCGGATTATCTTTGCAT29470.20700254415749894No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACC28670.2013832012553612No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC28280.19864377159056903No Hit
TCATCAAAAGTGGCACGATCAACCAAGGTAAAAATGCAGAAAGCAGTAAAGCAATTGGAAT24960.17532349854669746No Hit
GGTAAGCTATTTAAGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCAC22300.15663918339708946No Hit
GATAGGTTTTAAATAACGCCATCAGTGCAATTAATACA15710.11034984624072985No Hit
TGCGGCAAGATGCCCTGTGAGTTTAATTTCAATCCCTTTCCCTGCCTTTAACCAATCAAAA15660.10999863730934623No Hit
CTTGGAAGCATGACGCCGATTTCTTGCAAGTTAGGTGCGATGTTATCAGCAATATTGGGTA14560.10227204081890684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)