FastQCFastQC Report
Thu 4 May 2023
TP2_S2_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP2_S2_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187457
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT116410.980330235115882No Hit
TGTGGCATCGTAGGAAGTTTGCTTTCTACTGCTCGAGGTGGATGGGTGGCATTACCTGTCT27830.2343663812668585No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACC18830.15857416310653777No Hit
TTTATTGGTGGCATTTTTTATGCCTTTTTTAATCCCTATTATCGTCGGATTATCTTTGCAT18730.15773202734920083No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC18700.15747938662199978No Hit
TTCTTCTAAGGTCTCAATCGCTTCTAAACATTTTTGACGACGCGTTAAATAAATTGCGGCT17360.14619476747368534No Hit
AATCAAAAAAGCCCTAACACAGGTAAACCAATTAACTGTGTAAACTGTCACGGTAATATTT13890.11697265669409501No Hit
TCATGCGCGTATTAAAATTTGGTGGCACTTCATTAGCCAACCCTGAGCGTTTCTCGCAAGC13400.11284619148314423No Hit
ACTGTGTAAACTGTCACGGTAATATTTCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA12820.10796180409059022TruSeq Adapter, Index 7 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)