FastQCFastQC Report
Thu 4 May 2023
TP1_S1_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTP1_S1_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494394
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGAT202921.357874830867897No Hit
TCATGCGCGTATTAAAATTTGGTGGCACTTCATTAGCCAACCCTGAGCGTTTCTCGCAAGC32940.22042379720475322No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAAC30100.2014194382472092No Hit
TTTATTGGTGGCATTTTTTATGCCTTTTTTAATCCCTATTATCGTCGGATTATCTTTGCAT30000.20075027067828163No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACC29000.19405859498900557No Hit
TCATCAAAAGTGGCACGATCAACCAAGGTAAAAATGCAGAAAGCAGTAAAGCAATTGGAAT25270.1690986446680059No Hit
GGTAAGCTATTTAAGTCCGCAAGTTTGGTGGTTATTTTCTCTTTATAGATCGGAAGAGCAC22630.1514326208483171No Hit
TGCGGCAAGATGCCCTGTGAGTTTAATTTCAATCCCTTTCCCTGCCTTTAACCAATCAAAA16050.10740139481288068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)