FastQCFastQC Report
Mon 25 Sep 2023
Tp9_S9_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp9_S9_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15135131
Sequences flagged as poor quality0
Sequence length63-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATCCACAGCAAGCGGCATTGTGTCTATTGCTCATGGTAACGCCGCTAAAGCTACAGGTGATGGCTCTATTG444930.29397168746012176No Hit
CAACAGCATTGGCTTAATCCCATTCAAGGTTGCTATCGTTTTCATTTTGA403980.26691543006796575No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG388800.2568857844705804No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC308050.20353309132243388No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT293720.19406505302134483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)