FastQCFastQC Report
Mon 25 Sep 2023
Tp8_S8_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp8_S8_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25329418
Sequences flagged as poor quality0
Sequence length63-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAGCATTGGCTTAATCCCATTCAAGGTTGCTATCGTTTTCATTTTGA933960.36872540853485064No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG769550.3038166925114505No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT516620.20396046999579695No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC465420.18374681960714612No Hit
CTCATCCACAGCAAGCGGCATTGTGTCTATTGCTCATGGTAACGCCGCTAAAGCTACAGGTGATGGCTCTATTG399570.15774938058189888No Hit
TAATCACCTGCTACACGTACATTACCTAATTTGTAACCTACTGCAACACGTGGCTCAATTTTAGTTTTGCTTGAA380020.15003108243545116No Hit
CATGGATTCTGACGGTGATATTGTGAAGGCAGAACAACGTCTGAATTACAGTCGCCTTGCTCAAACTTTATTGAA271480.10717972280294794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)