FastQCFastQC Report
Thu 11 May 2023
Tp3_S3_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp3_S3_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10832584
Sequences flagged as poor quality0
Sequence length64-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC2385792.202420032007137No Hit
CCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAA317800.2933741386173419No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC254860.2352716581750024No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG156010.14401919246598965No Hit
CTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTG146250.13500933849209018No Hit
CCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA128580.11869744097991763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)