FastQCFastQC Report
Thu 11 May 2023
Tp2_S2_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp2_S2_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7940212
Sequences flagged as poor quality0
Sequence length63-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC1276191.607249277475211No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC261100.3288325299122996No Hit
CCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAA251150.316301378351107No Hit
CTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTG126400.15918970425474788No Hit
CCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA108590.13675957266632174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)