FastQCFastQC Report
Thu 11 May 2023
Tp1_S1_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp1_S1_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3531888
Sequences flagged as poor quality0
Sequence length63-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC689501.9522136602293165No Hit
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGC112260.31784699854582027No Hit
CCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAA96700.27379124139836825No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG74230.2101708774457174No Hit
ATTAAAAATGAAAATTGGTCAGCTAGCCAATATTGTTGGCTGTACGGTGGAAGCCGTACGTTTCTATGAAAAACA59590.1687199594098114No Hit
CTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTG39830.1127725454487798No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACCATTGGTGAGTGTGT38390.10869540596983823No Hit
CCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA37830.1071098517280276No Hit
TTTATTGGTGGCATTTTTTATGCCTTTTTTAATCCCTATTATCGTCGGATTATCTTTGCATTTTGTTGATTT36710.1039387432444064No Hit
TCATGCGCGTATTAAAATTTGGTGGCACTTCATTAGCCAACCCTGAGCGTTTCTCGCAAGCGGCTCAATTAATCG35610.10082426169799269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)