This is cutadapt 2.8 with Python 3.7.4 Command line parameters: -a C{9} -m 20 -o TP20.trim.fastq TP20.fastq Processing reads on 1 core in single-end mode ... Finished in 55.66 s (11 us/read; 5.56 M reads/minute). === Summary === Total reads processed: 5,161,948 Reads with adapters: 468,973 (9.1%) Reads that were too short: 18,484 (0.4%) Reads written (passing filters): 5,143,464 (99.6%) Total basepairs processed: 306,939,042 bp Total written (filtered): 300,245,172 bp (97.8%) === Adapter 1 === Sequence: CCCCCCCCC; Type: regular 3'; Length: 9; Trimmed: 468973 times; Reverse-complemented: 0 times No. of allowed errors: 0-9 bp: 0 Bases preceding removed adapters: A: 54.2% C: 0.0% G: 16.2% T: 29.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 93809 80655.4 0 93809 4 46395 20163.9 0 46395 5 29350 5041.0 0 29350 6 20825 1260.2 0 20825 7 18098 315.1 0 18098 8 14278 78.8 0 14278 9 13569 19.7 0 13569 10 15321 19.7 0 15321 11 17547 19.7 0 17547 12 11029 19.7 0 11029 13 9491 19.7 0 9491 14 9271 19.7 0 9271 15 8474 19.7 0 8474 16 8997 19.7 0 8997 17 8736 19.7 0 8736 18 8560 19.7 0 8560 19 9107 19.7 0 9107 20 8405 19.7 0 8405 21 7457 19.7 0 7457 22 6853 19.7 0 6853 23 6128 19.7 0 6128 24 6273 19.7 0 6273 25 6811 19.7 0 6811 26 6610 19.7 0 6610 27 6335 19.7 0 6335 28 5916 19.7 0 5916 29 5915 19.7 0 5915 30 5349 19.7 0 5349 31 5277 19.7 0 5277 32 5224 19.7 0 5224 33 4925 19.7 0 4925 34 4345 19.7 0 4345 35 3439 19.7 0 3439 36 2682 19.7 0 2682 37 2617 19.7 0 2617 38 2477 19.7 0 2477 39 2735 19.7 0 2735 40 2499 19.7 0 2499 41 2078 19.7 0 2078 42 1957 19.7 0 1957 43 1992 19.7 0 1992 44 1968 19.7 0 1968 45 1798 19.7 0 1798 46 1693 19.7 0 1693 47 1637 19.7 0 1637 48 1363 19.7 0 1363 49 1026 19.7 0 1026 50 861 19.7 0 861 51 560 19.7 0 560 52 337 19.7 0 337 53 232 19.7 0 232 54 143 19.7 0 143 55 81 19.7 0 81 56 43 19.7 0 43 57 21 19.7 0 21 58 24 19.7 0 24 59 17 19.7 0 17 60 13 19.7 0 13