Loading required package: S4Vectors
Loading required package: stats4
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Loading required package: parallel

Attaching package: ‘BiocGenerics’

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Attaching package: ‘S4Vectors’

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Loading required package: IRanges
Loading required package: GenomicRanges
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Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: DelayedArray
Loading required package: matrixStats

Attaching package: ‘matrixStats’

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Loading required package: BiocParallel

Attaching package: ‘DelayedArray’

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estimating size factors
estimating dispersions
gene-wise dispersion estimates
mean-dispersion relationship
final dispersion estimates
fitting model and testing
null device 
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Loading required package: ggplot2
Loading required package: ggrepel
null device 
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Attaching package: ‘dplyr’

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Warning message:
Column `id` joining factors with different levels, coercing to character vector 
[1] "fold_change"    "id"             "pvalue"         "padj"          
[5] "log2FoldChange" "gff_annotation"
Error in file(file, "rt") : invalid 'description' argument
Calls: read.csv -> read.table -> file
Execution halted
