Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11402:25061:10778 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11402:25061:10778 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11408:24871:5965 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11408:24871:5965 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11409:6155:9206 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11409:6155:9206 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11412:13011:12049 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11412:13011:12049 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11506:18863:10444 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11506:18863:10444 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11606:15289:7782 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11606:15289:7782 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11607:15317:11133 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11607:15317:11133 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11608:1668:11174 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11608:1668:11174 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12409:3351:1573 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12409:3351:1573 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:15890:15964 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:15890:15964 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:21010:5121 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:21010:5121 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12412:17498:9519 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12412:17498:9519 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12412:21148:3659 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12412:21148:3659 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12508:6034:14336 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12508:6034:14336 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12601:3877:3147 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12601:3877:3147 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12611:23298:2696 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12611:23298:2696 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13408:13205:7727 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13408:13205:7727 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13503:6427:4883 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13503:6427:4883 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13506:12459:11660 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13506:12459:11660 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13509:7530:13742 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13509:7530:13742 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13601:14150:4862 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13601:14150:4862 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13601:21917:12110 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13601:21917:12110 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13605:23911:18218 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13605:23911:18218 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13609:18840:5600 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13609:18840:5600 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13609:8758:10497 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13609:8758:10497 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21410:13483:8400 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21410:13483:8400 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21505:21721:3336 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21505:21721:3336 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21508:21269:7495 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21508:21269:7495 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21602:26289:5832 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21602:26289:5832 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22401:25750:4152 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22401:25750:4152 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22409:13038:19983 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22409:13038:19983 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22411:20612:1289 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22411:20612:1289 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22509:4894:15093 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22509:4894:15093 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22510:8068:20397 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22510:8068:20397 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22611:17471:13265 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22611:17471:13265 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22612:14326:1136 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22612:14326:1136 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23402:17559:4311 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23402:17559:4311 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23408:4046:8790 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23408:4046:8790 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23501:20506:8328 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23501:20506:8328 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23510:22608:4498 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23510:22608:4498 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23510:5767:4874 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23510:5767:4874 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:1522:2238 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:1522:2238 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11201:9160:20283 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11201:9160:20283 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11204:7375:14211 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11204:7375:14211 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11209:3700:9543 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11209:3700:9543 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11307:18383:4200 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11307:18383:4200 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11307:18498:15813 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11307:18498:15813 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12101:13364:18623 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12101:13364:18623 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12105:16675:5419 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12105:16675:5419 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12111:24285:14165 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12111:24285:14165 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12303:19677:10126 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12303:19677:10126 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12306:10344:14123 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12306:10344:14123 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12307:24648:20289 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12307:24648:20289 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12308:9183:9681 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12308:9183:9681 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13206:15232:6139 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13206:15232:6139 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13301:8448:2202 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13301:8448:2202 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13311:20762:18000 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13311:20762:18000 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21201:12092:8613 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21201:12092:8613 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21202:12205:13195 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21202:12205:13195 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21202:26222:4443 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21202:26222:4443 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21203:5962:5682 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21203:5962:5682 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21205:3482:16840 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21205:3482:16840 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21210:23636:2933 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21210:23636:2933 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21212:16143:12028 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21212:16143:12028 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22104:10638:18631 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22104:10638:18631 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22201:4479:16355 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22201:4479:16355 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22202:10378:7268 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22202:10378:7268 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22211:23047:18649 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22211:23047:18649 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22302:4432:17140 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22302:4432:17140 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23101:22772:9601 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23101:22772:9601 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23104:10308:6652 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23104:10308:6652 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23301:24399:18627 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23301:24399:18627 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23301:5189:10818 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23301:5189:10818 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23310:7558:17437 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23310:7558:17437 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23311:6437:18219 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23311:6437:18219 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11501:23328:10989 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11501:23328:10989 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11610:5787:10996 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11610:5787:10996 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12405:8327:8357 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12405:8327:8357 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12411:7121:9115 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12411:7121:9115 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12412:14105:4338 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12412:14105:4338 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12412:4063:11743 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12412:4063:11743 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12606:2361:3908 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12606:2361:3908 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13401:14144:5697 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13401:14144:5697 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13412:22247:9627 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13412:22247:9627 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13501:14711:10076 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13501:14711:10076 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13508:14002:16085 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13508:14002:16085 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13510:6802:17757 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13510:6802:17757 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13609:21309:17618 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13609:21309:17618 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21401:18798:11090 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21401:18798:11090 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21408:22468:15210 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21408:22468:15210 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21502:4836:3285 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21502:4836:3285 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21506:25708:16770 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21506:25708:16770 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22402:1406:12046 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22402:1406:12046 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22402:3003:2905 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22402:3003:2905 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22406:10393:9781 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22406:10393:9781 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22407:19315:17664 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22407:19315:17664 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22505:4037:3712 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22505:4037:3712 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22506:17891:7182 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22506:17891:7182 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22512:11268:11008 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22512:11268:11008 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23401:4635:2697 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23401:4635:2697 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23404:12130:19903 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23404:12130:19903 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23404:21325:2382 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23404:21325:2382 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23406:9323:14463 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23406:9323:14463 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23407:9722:6667 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23407:9722:6667 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23410:7187:6127 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23410:7187:6127 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23412:12088:13946 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23412:12088:13946 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23511:10147:10973 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23511:10147:10973 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23605:26050:8590 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23605:26050:8590 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23611:23063:8637 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23611:23063:8637 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11106:16853:18513 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11106:16853:18513 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11108:5648:13711 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11108:5648:13711 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11109:2633:13249 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11109:2633:13249 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11211:17188:5523 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11211:17188:5523 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12101:9525:11994 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12101:9525:11994 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12107:11186:19888 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12107:11186:19888 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12201:25205:1134 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12201:25205:1134 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12302:25078:10666 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12302:25078:10666 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12309:5215:6536 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12309:5215:6536 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13101:12076:10316 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13101:12076:10316 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13108:2806:2467 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13108:2806:2467 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13201:17833:13288 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13201:17833:13288 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13303:10841:4205 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13303:10841:4205 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13307:3744:16642 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13307:3744:16642 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21105:16295:19660 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21105:16295:19660 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21106:9775:11238 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21106:9775:11238 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21204:16199:1105 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21204:16199:1105 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21210:25160:10034 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21210:25160:10034 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22102:4681:10263 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22102:4681:10263 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22109:3118:17679 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22109:3118:17679 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22205:20584:2684 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22205:20584:2684 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22206:13055:8690 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22206:13055:8690 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22211:23696:2843 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22211:23696:2843 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22212:25808:9105 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22212:25808:9105 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23102:12733:18841 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23102:12733:18841 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23107:4997:2662 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23107:4997:2662 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23111:15892:10776 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23111:15892:10776 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23201:19887:14247 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23201:19887:14247 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23307:21644:12936 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23307:21644:12936 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23309:21999:18104 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23309:21999:18104 2:N:0:GTCTTAGT' because it was < 2 characters long 8429465 reads; of these: 8429465 (100.00%) were paired; of these: 1519531 (18.03%) aligned concordantly 0 times 847942 (10.06%) aligned concordantly exactly 1 time 6061992 (71.91%) aligned concordantly >1 times ---- 1519531 pairs aligned concordantly 0 times; of these: 6924 (0.46%) aligned discordantly 1 time ---- 1512607 pairs aligned 0 times concordantly or discordantly; of these: 3025214 mates make up the pairs; of these: 2175785 (71.92%) aligned 0 times 37305 (1.23%) aligned exactly 1 time 812124 (26.85%) aligned >1 times 87.09% overall alignment rate Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11404:12364:17923 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11404:12364:17923 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11404:17430:14212 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11404:17430:14212 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11408:10270:8587 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11408:10270:8587 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11408:9828:15664 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11408:9828:15664 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11611:8171:14634 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11611:8171:14634 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12408:23869:16361 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12408:23869:16361 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:16124:6106 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:16124:6106 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12506:5121:14889 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12506:5121:14889 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13404:13168:19189 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13404:13168:19189 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13407:18037:17627 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13407:18037:17627 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13408:11655:5964 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13408:11655:5964 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13503:12586:15790 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13503:12586:15790 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13509:17255:18141 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13509:17255:18141 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13608:11989:6141 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13608:11989:6141 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13612:16700:12051 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13612:16700:12051 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21401:19530:2100 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21401:19530:2100 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22401:18059:9263 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22401:18059:9263 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22402:22481:14814 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22402:22481:14814 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22408:2169:19947 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22408:2169:19947 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22506:5940:8498 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22506:5940:8498 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22509:17900:8733 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22509:17900:8733 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22609:21111:18022 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22609:21111:18022 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23510:2972:11121 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23510:2972:11121 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23512:26834:18930 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23512:26834:18930 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11102:7474:3272 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11102:7474:3272 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:4631:19399 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:4631:19399 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11108:16843:9824 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11108:16843:9824 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11304:15336:3254 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11304:15336:3254 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11307:16927:11936 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11307:16927:11936 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12105:11320:18516 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12105:11320:18516 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12106:5418:18184 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12106:5418:18184 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12107:24772:3472 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12107:24772:3472 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12111:25986:15838 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12111:25986:15838 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12212:18290:1644 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12212:18290:1644 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12305:9949:2965 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12305:9949:2965 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13103:19489:10980 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13103:19489:10980 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13109:24103:18549 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13109:24103:18549 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13111:15677:19490 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13111:15677:19490 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13207:22165:11959 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13207:22165:11959 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21104:10580:6731 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21104:10580:6731 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21112:4307:9443 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21112:4307:9443 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21205:23074:13779 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21205:23074:13779 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21305:19216:10712 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21305:19216:10712 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21310:2702:13190 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21310:2702:13190 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22103:10029:4687 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22103:10029:4687 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22107:25126:7186 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22107:25126:7186 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22203:25068:1946 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22203:25068:1946 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22205:5621:8193 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22205:5621:8193 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22304:2895:14126 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22304:2895:14126 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23104:19190:4946 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23104:19190:4946 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23201:21336:9827 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23201:21336:9827 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11404:22877:1844 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11404:22877:1844 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11410:17674:2531 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11410:17674:2531 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11610:17667:7493 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11610:17667:7493 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12402:12843:7084 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12402:12843:7084 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12505:20266:16282 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12505:20266:16282 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12604:9225:3805 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12604:9225:3805 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12612:21017:19758 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12612:21017:19758 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13404:7143:8956 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13404:7143:8956 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13405:23567:17702 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13405:23567:17702 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13505:11133:12888 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13505:11133:12888 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13606:17945:3728 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13606:17945:3728 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13609:25374:3817 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13609:25374:3817 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21403:16465:20375 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21403:16465:20375 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21406:13419:13025 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21406:13419:13025 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21504:4268:4823 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21504:4268:4823 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21509:12672:4900 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21509:12672:4900 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22403:11857:1785 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22403:11857:1785 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22405:20398:12275 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22405:20398:12275 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22406:17035:2132 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22406:17035:2132 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22501:2317:10352 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22501:2317:10352 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22509:15718:16587 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22509:15718:16587 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:3844:10631 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:3844:10631 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22605:14773:4509 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22605:14773:4509 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22608:17612:9720 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22608:17612:9720 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23411:25285:2657 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23411:25285:2657 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23502:17708:16179 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23502:17708:16179 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23502:25500:13992 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23502:25500:13992 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23601:16710:5352 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23601:16710:5352 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23603:23304:4408 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23603:23304:4408 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23609:24920:2468 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23609:24920:2468 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11202:1444:11895 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11202:1444:11895 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11204:14216:3469 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11204:14216:3469 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11206:7508:1604 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11206:7508:1604 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11210:16259:4450 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11210:16259:4450 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11211:16613:17252 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11211:16613:17252 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12109:19234:11563 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12109:19234:11563 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12110:24337:6337 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12110:24337:6337 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12206:20595:8547 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12206:20595:8547 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12207:24852:5603 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12207:24852:5603 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13111:5485:8951 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13111:5485:8951 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13312:24593:13939 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13312:24593:13939 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21104:12226:3317 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21104:12226:3317 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21111:4360:18089 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21111:4360:18089 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21205:1601:19163 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21205:1601:19163 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21308:1437:18657 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21308:1437:18657 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22106:17517:18883 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22106:17517:18883 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22108:2145:14450 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22108:2145:14450 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22206:6158:1147 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22206:6158:1147 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22301:26032:2422 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22301:26032:2422 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23108:20352:6685 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23108:20352:6685 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23109:11581:5802 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23109:11581:5802 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23111:5308:3761 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23111:5308:3761 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23304:17204:8547 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23304:17204:8547 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23312:14320:18238 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23312:14320:18238 2:N:0:GTCTTAGT' because it was < 2 characters long 6616423 reads; of these: 6616423 (100.00%) were paired; of these: 1092755 (16.52%) aligned concordantly 0 times 725468 (10.96%) aligned concordantly exactly 1 time 4798200 (72.52%) aligned concordantly >1 times ---- 1092755 pairs aligned concordantly 0 times; of these: 8967 (0.82%) aligned discordantly 1 time ---- 1083788 pairs aligned 0 times concordantly or discordantly; of these: 2167576 mates make up the pairs; of these: 1486245 (68.57%) aligned 0 times 30817 (1.42%) aligned exactly 1 time 650514 (30.01%) aligned >1 times 88.77% overall alignment rate Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11402:10080:19145 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11402:10080:19145 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11503:11305:17263 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11503:11305:17263 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11506:10621:7856 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11506:10621:7856 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11601:16398:17680 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11601:16398:17680 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11606:19957:13185 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11606:19957:13185 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11606:23639:13827 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11606:23639:13827 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12405:20553:20308 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12405:20553:20308 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:10888:18093 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12411:10888:18093 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12412:13805:10030 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12412:13805:10030 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12606:19087:14031 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12606:19087:14031 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13401:3025:19461 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13401:3025:19461 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13407:21114:19882 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13407:21114:19882 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13411:21555:2943 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13411:21555:2943 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13501:4674:4712 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13501:4674:4712 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13503:25941:17362 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13503:25941:17362 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13509:7062:7259 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13509:7062:7259 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13511:4343:7736 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13511:4343:7736 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13512:12549:16645 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13512:12549:16645 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13612:13999:14191 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13612:13999:14191 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21405:13408:3641 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21405:13408:3641 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21406:13921:5612 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21406:13921:5612 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21408:12811:8778 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21408:12811:8778 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21409:11194:3954 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21409:11194:3954 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21411:26582:3079 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21411:26582:3079 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21607:14935:15300 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21607:14935:15300 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21610:17440:10396 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21610:17440:10396 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22402:10549:10173 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22402:10549:10173 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22403:17440:10033 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22403:17440:10033 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22406:12829:20213 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22406:12829:20213 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22406:18578:16534 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22406:18578:16534 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22406:23488:9918 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22406:23488:9918 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22410:22789:8604 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22410:22789:8604 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22410:24122:11743 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22410:24122:11743 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22602:13812:3427 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22602:13812:3427 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23406:25085:2722 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23406:25085:2722 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23408:9789:11548 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23408:9789:11548 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:19828:7153 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:19828:7153 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11204:14221:2726 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11204:14221:2726 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11205:3041:15166 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11205:3041:15166 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12105:10886:17878 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12105:10886:17878 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12112:9973:13781 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12112:9973:13781 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12208:7095:4558 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12208:7095:4558 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12211:11013:8270 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12211:11013:8270 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12211:24432:9574 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12211:24432:9574 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12307:2631:13323 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12307:2631:13323 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13110:16245:4247 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13110:16245:4247 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13208:19064:2488 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13208:19064:2488 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13209:5400:1556 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13209:5400:1556 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13310:25372:6405 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13310:25372:6405 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21104:13617:19257 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21104:13617:19257 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21105:8182:18326 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21105:8182:18326 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21111:9422:12697 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21111:9422:12697 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21207:2798:9618 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21207:2798:9618 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21210:16794:20355 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21210:16794:20355 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21312:2889:11560 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21312:2889:11560 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21312:8068:5168 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21312:8068:5168 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22103:24710:8788 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22103:24710:8788 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22103:9366:8922 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22103:9366:8922 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22104:6184:3156 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22104:6184:3156 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22108:5504:6328 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22108:5504:6328 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22109:15774:6077 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22109:15774:6077 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22202:21811:4960 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22202:21811:4960 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22204:26186:7099 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22204:26186:7099 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22210:25355:16712 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22210:25355:16712 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22212:25269:3909 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22212:25269:3909 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22308:20170:6979 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22308:20170:6979 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22312:10586:19012 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22312:10586:19012 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23312:7760:20271 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23312:7760:20271 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11408:25676:3901 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11408:25676:3901 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11409:22708:15745 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11409:22708:15745 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11501:13217:8459 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11501:13217:8459 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11503:7313:15553 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11503:7313:15553 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11601:17633:3860 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11601:17633:3860 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11607:3921:12591 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11607:3921:12591 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11610:7774:15233 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11610:7774:15233 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11612:4588:13816 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11612:4588:13816 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12403:24921:7938 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12403:24921:7938 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12404:6619:3403 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12404:6619:3403 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12406:1392:8679 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12406:1392:8679 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12408:7581:18080 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12408:7581:18080 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12412:18343:1233 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12412:18343:1233 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12602:16654:12722 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:12602:16654:12722 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13406:11083:2072 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13406:11083:2072 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13411:17373:17498 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13411:17373:17498 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13507:25265:15141 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13507:25265:15141 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13509:1046:15321 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13509:1046:15321 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13601:2886:7767 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13601:2886:7767 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13601:8148:2430 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13601:8148:2430 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13604:10038:14740 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13604:10038:14740 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13606:2048:11472 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:13606:2048:11472 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21401:24836:11480 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21401:24836:11480 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21405:1409:2603 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21405:1409:2603 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21408:9660:16055 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21408:9660:16055 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21410:1113:6408 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21410:1113:6408 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21412:20008:4294 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21412:20008:4294 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21503:14826:20369 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21503:14826:20369 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21602:3292:16628 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21602:3292:16628 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22410:18625:19371 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22410:18625:19371 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22509:15218:17854 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22509:15218:17854 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:21184:6621 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:21184:6621 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:21384:8096 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:21384:8096 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:6086:18496 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22510:6086:18496 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22602:5116:10513 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22602:5116:10513 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22605:7684:11350 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22605:7684:11350 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23409:24981:2233 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23409:24981:2233 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23508:16735:11105 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23508:16735:11105 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23511:25494:2727 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23511:25494:2727 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23604:14156:13133 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23604:14156:13133 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23604:5818:6070 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23604:5818:6070 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23607:13515:14979 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23607:13515:14979 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23610:23808:4218 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23610:23808:4218 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11101:22493:5621 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11101:22493:5621 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11108:12189:16839 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11108:12189:16839 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11112:7492:3788 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11112:7492:3788 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11205:20232:19255 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:11205:20232:19255 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12102:12934:6864 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12102:12934:6864 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12108:11530:1372 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12108:11530:1372 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12112:23154:10552 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12112:23154:10552 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12205:1373:14691 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12205:1373:14691 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12306:17838:8269 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12306:17838:8269 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12309:16410:2125 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12309:16410:2125 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13112:19939:7259 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13112:19939:7259 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13206:23703:15810 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13206:23703:15810 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21202:7979:13301 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21202:7979:13301 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21309:2853:19669 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21309:2853:19669 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21312:3535:13870 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21312:3535:13870 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22103:3501:6589 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22103:3501:6589 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22312:14544:13146 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:22312:14544:13146 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23104:10896:2398 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23104:10896:2398 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23107:16298:18505 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23107:16298:18505 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23206:5813:1768 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23206:5813:1768 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23304:23690:4340 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23304:23690:4340 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23306:21317:13847 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23306:21317:13847 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23311:7219:16005 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23311:7219:16005 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23312:7238:6903 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23312:7238:6903 2:N:0:GTCTTAGT' because it was < 2 characters long 8616949 reads; of these: 8616949 (100.00%) were paired; of these: 2519439 (29.24%) aligned concordantly 0 times 461197 (5.35%) aligned concordantly exactly 1 time 5636313 (65.41%) aligned concordantly >1 times ---- 2519439 pairs aligned concordantly 0 times; of these: 4091 (0.16%) aligned discordantly 1 time ---- 2515348 pairs aligned 0 times concordantly or discordantly; of these: 5030696 mates make up the pairs; of these: 4259950 (84.68%) aligned 0 times 23574 (0.47%) aligned exactly 1 time 747172 (14.85%) aligned >1 times 75.28% overall alignment rate Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11401:15591:15042 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11401:15591:15042 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11404:19685:15092 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11404:19685:15092 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11509:3926:1789 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:11509:3926:1789 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12405:15724:12480 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12405:15724:12480 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12508:18680:14014 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12508:18680:14014 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12511:4805:10087 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:12511:4805:10087 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13609:14068:5940 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:13609:14068:5940 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21507:18990:18354 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21507:18990:18354 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21512:11475:5761 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:21512:11475:5761 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22402:8870:15817 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22402:8870:15817 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22405:23017:19949 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22405:23017:19949 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22504:11002:17292 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:22504:11002:17292 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23405:2704:5968 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23405:2704:5968 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23511:18956:5751 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23511:18956:5751 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23603:4736:15054 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:3:23603:4736:15054 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:21512:13274 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:11107:21512:13274 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12205:17392:15091 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12205:17392:15091 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12206:2575:14948 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:12206:2575:14948 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13109:12833:9457 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13109:12833:9457 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13208:14561:5557 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13208:14561:5557 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13305:9526:15387 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:13305:9526:15387 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21207:22893:12226 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21207:22893:12226 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21211:16166:1700 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21211:16166:1700 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21303:26232:19334 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:21303:26232:19334 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22301:19401:14405 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:22301:19401:14405 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23308:26281:2687 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:1:23308:26281:2687 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11405:3380:9340 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:11405:3380:9340 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21407:18250:3329 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21407:18250:3329 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21601:20570:2372 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:21601:20570:2372 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22509:16003:14987 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:22509:16003:14987 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23509:9829:19182 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23509:9829:19182 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23510:20683:15136 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:4:23510:20683:15136 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12105:6742:19591 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12105:6742:19591 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12302:3655:7000 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12302:3655:7000 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12309:19345:17562 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:12309:19345:17562 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13104:4376:9425 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13104:4376:9425 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13207:10676:15230 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13207:10676:15230 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13309:9904:17778 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13309:9904:17778 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13310:15583:12194 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:13310:15583:12194 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21105:18235:4191 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21105:18235:4191 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21112:16759:16509 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21112:16759:16509 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21203:24446:8318 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21203:24446:8318 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21204:26744:12547 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21204:26744:12547 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21302:15629:12361 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:21302:15629:12361 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23101:12084:5324 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23101:12084:5324 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23102:14527:6131 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23102:14527:6131 2:N:0:GTCTTAGT' because it was < 2 characters long Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23103:13882:14968 2:N:0:GTCTTAGT' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SL-NXC:HHNFTBGX5180320:HHNFTBGX5:2:23103:13882:14968 2:N:0:GTCTTAGT' because it was < 2 characters long 3357688 reads; of these: 3357688 (100.00%) were paired; of these: 989828 (29.48%) aligned concordantly 0 times 126293 (3.76%) aligned concordantly exactly 1 time 2241567 (66.76%) aligned concordantly >1 times ---- 989828 pairs aligned concordantly 0 times; of these: 1147 (0.12%) aligned discordantly 1 time ---- 988681 pairs aligned 0 times concordantly or discordantly; of these: 1977362 mates make up the pairs; of these: 1660955 (84.00%) aligned 0 times 7570 (0.38%) aligned exactly 1 time 308837 (15.62%) aligned >1 times 75.27% overall alignment rate 1985 GFF lines processed. 100000 SAM alignment record pairs processed. 200000 SAM alignment record pairs processed. 300000 SAM alignment record pairs processed. 400000 SAM alignment record pairs processed. 500000 SAM alignment record pairs processed. 600000 SAM alignment record pairs processed. 700000 SAM alignment record pairs processed. 800000 SAM alignment record pairs processed. 900000 SAM alignment record pairs processed. 1000000 SAM alignment record pairs processed. 1100000 SAM alignment record pairs processed. 1200000 SAM alignment record pairs processed. 1300000 SAM alignment record pairs processed. 1400000 SAM alignment record pairs processed. 1500000 SAM alignment record pairs processed. 1600000 SAM alignment record pairs processed. 1700000 SAM alignment record pairs processed. 1800000 SAM alignment record pairs processed. 1900000 SAM alignment record pairs processed. 2000000 SAM alignment record pairs processed. 2100000 SAM alignment record pairs processed. 2200000 SAM alignment record pairs processed. 2300000 SAM alignment record pairs processed. 2400000 SAM alignment record pairs processed. 2500000 SAM alignment record pairs processed. 2600000 SAM alignment record pairs processed. 2700000 SAM alignment record pairs processed. 2800000 SAM alignment record pairs processed. 2900000 SAM alignment record pairs processed. 3000000 SAM alignment record pairs processed. 3100000 SAM alignment record pairs processed. 3200000 SAM alignment record pairs processed. 3300000 SAM alignment record pairs processed. 3400000 SAM alignment record pairs processed. 3500000 SAM alignment record pairs processed. 3600000 SAM alignment record pairs processed. 3700000 SAM alignment record pairs processed. 3800000 SAM alignment record pairs processed. 3900000 SAM alignment record pairs processed. 4000000 SAM alignment record pairs processed. 4100000 SAM alignment record pairs processed. 4200000 SAM alignment record pairs processed. 4300000 SAM alignment record pairs processed. 4400000 SAM alignment record pairs processed. 4500000 SAM alignment record pairs processed. 4600000 SAM alignment record pairs processed. 4700000 SAM alignment record pairs processed. 4800000 SAM alignment record pairs processed. 4900000 SAM alignment record pairs processed. 5000000 SAM alignment record pairs processed. 5100000 SAM alignment record pairs processed. 5200000 SAM alignment record pairs processed. 5300000 SAM alignment record pairs processed. 5400000 SAM alignment record pairs processed. 5500000 SAM alignment record pairs processed. 5600000 SAM alignment record pairs processed. 5700000 SAM alignment record pairs processed. 5800000 SAM alignment record pairs processed. 5900000 SAM alignment record pairs processed. 6000000 SAM alignment record pairs processed. 6100000 SAM alignment record pairs processed. 6200000 SAM alignment record pairs processed. 6300000 SAM alignment record pairs processed. 6400000 SAM alignment record pairs processed. 6500000 SAM alignment record pairs processed. 6600000 SAM alignment record pairs processed. 6700000 SAM alignment record pairs processed. 6800000 SAM alignment record pairs processed. 6900000 SAM alignment record pairs processed. 7000000 SAM alignment record pairs processed. 7100000 SAM alignment record pairs processed. 7200000 SAM alignment record pairs processed. 7300000 SAM alignment record pairs processed. 7400000 SAM alignment record pairs processed. 7500000 SAM alignment record pairs processed. 7600000 SAM alignment record pairs processed. 7700000 SAM alignment record pairs processed. 7800000 SAM alignment record pairs processed. 7900000 SAM alignment record pairs processed. 8000000 SAM alignment record pairs processed. 8100000 SAM alignment record pairs processed. 8200000 SAM alignment record pairs processed. 8300000 SAM alignment record pairs processed. 8400000 SAM alignment record pairs processed. 8429465 SAM alignment pairs processed. 1985 GFF lines processed. 100000 SAM alignment record pairs processed. 200000 SAM alignment record pairs processed. 300000 SAM alignment record pairs processed. 400000 SAM alignment record pairs processed. 500000 SAM alignment record pairs processed. 600000 SAM alignment record pairs processed. 700000 SAM alignment record pairs processed. 800000 SAM alignment record pairs processed. 900000 SAM alignment record pairs processed. 1000000 SAM alignment record pairs processed. 1100000 SAM alignment record pairs processed. 1200000 SAM alignment record pairs processed. 1300000 SAM alignment record pairs processed. 1400000 SAM alignment record pairs processed. 1500000 SAM alignment record pairs processed. 1600000 SAM alignment record pairs processed. 1700000 SAM alignment record pairs processed. 1800000 SAM alignment record pairs processed. 1900000 SAM alignment record pairs processed. 2000000 SAM alignment record pairs processed. 2100000 SAM alignment record pairs processed. 2200000 SAM alignment record pairs processed. 2300000 SAM alignment record pairs processed. 2400000 SAM alignment record pairs processed. 2500000 SAM alignment record pairs processed. 2600000 SAM alignment record pairs processed. 2700000 SAM alignment record pairs processed. 2800000 SAM alignment record pairs processed. 2900000 SAM alignment record pairs processed. 3000000 SAM alignment record pairs processed. 3100000 SAM alignment record pairs processed. 3200000 SAM alignment record pairs processed. 3300000 SAM alignment record pairs processed. 3400000 SAM alignment record pairs processed. 3500000 SAM alignment record pairs processed. 3600000 SAM alignment record pairs processed. 3700000 SAM alignment record pairs processed. 3800000 SAM alignment record pairs processed. 3900000 SAM alignment record pairs processed. 4000000 SAM alignment record pairs processed. 4100000 SAM alignment record pairs processed. 4200000 SAM alignment record pairs processed. 4300000 SAM alignment record pairs processed. 4400000 SAM alignment record pairs processed. 4500000 SAM alignment record pairs processed. 4600000 SAM alignment record pairs processed. 4700000 SAM alignment record pairs processed. 4800000 SAM alignment record pairs processed. 4900000 SAM alignment record pairs processed. 5000000 SAM alignment record pairs processed. 5100000 SAM alignment record pairs processed. 5200000 SAM alignment record pairs processed. 5300000 SAM alignment record pairs processed. 5400000 SAM alignment record pairs processed. 5500000 SAM alignment record pairs processed. 5600000 SAM alignment record pairs processed. 5700000 SAM alignment record pairs processed. 5800000 SAM alignment record pairs processed. 5900000 SAM alignment record pairs processed. 6000000 SAM alignment record pairs processed. 6100000 SAM alignment record pairs processed. 6200000 SAM alignment record pairs processed. 6300000 SAM alignment record pairs processed. 6400000 SAM alignment record pairs processed. 6500000 SAM alignment record pairs processed. 6600000 SAM alignment record pairs processed. 6616423 SAM alignment pairs processed. 1985 GFF lines processed. 100000 SAM alignment record pairs processed. 200000 SAM alignment record pairs processed. 300000 SAM alignment record pairs processed. 400000 SAM alignment record pairs processed. 500000 SAM alignment record pairs processed. 600000 SAM alignment record pairs processed. 700000 SAM alignment record pairs processed. 800000 SAM alignment record pairs processed. 900000 SAM alignment record pairs processed. 1000000 SAM alignment record pairs processed. 1100000 SAM alignment record pairs processed. 1200000 SAM alignment record pairs processed. 1300000 SAM alignment record pairs processed. 1400000 SAM alignment record pairs processed. 1500000 SAM alignment record pairs processed. 1600000 SAM alignment record pairs processed. 1700000 SAM alignment record pairs processed. 1800000 SAM alignment record pairs processed. 1900000 SAM alignment record pairs processed. 2000000 SAM alignment record pairs processed. 2100000 SAM alignment record pairs processed. 2200000 SAM alignment record pairs processed. 2300000 SAM alignment record pairs processed. 2400000 SAM alignment record pairs processed. 2500000 SAM alignment record pairs processed. 2600000 SAM alignment record pairs processed. 2700000 SAM alignment record pairs processed. 2800000 SAM alignment record pairs processed. 2900000 SAM alignment record pairs processed. 3000000 SAM alignment record pairs processed. 3100000 SAM alignment record pairs processed. 3200000 SAM alignment record pairs processed. 3300000 SAM alignment record pairs processed. 3400000 SAM alignment record pairs processed. 3500000 SAM alignment record pairs processed. 3600000 SAM alignment record pairs processed. 3700000 SAM alignment record pairs processed. 3800000 SAM alignment record pairs processed. 3900000 SAM alignment record pairs processed. 4000000 SAM alignment record pairs processed. 4100000 SAM alignment record pairs processed. 4200000 SAM alignment record pairs processed. 4300000 SAM alignment record pairs processed. 4400000 SAM alignment record pairs processed. 4500000 SAM alignment record pairs processed. 4600000 SAM alignment record pairs processed. 4700000 SAM alignment record pairs processed. 4800000 SAM alignment record pairs processed. 4900000 SAM alignment record pairs processed. 5000000 SAM alignment record pairs processed. 5100000 SAM alignment record pairs processed. 5200000 SAM alignment record pairs processed. 5300000 SAM alignment record pairs processed. 5400000 SAM alignment record pairs processed. 5500000 SAM alignment record pairs processed. 5600000 SAM alignment record pairs processed. 5700000 SAM alignment record pairs processed. 5800000 SAM alignment record pairs processed. 5900000 SAM alignment record pairs processed. 6000000 SAM alignment record pairs processed. 6100000 SAM alignment record pairs processed. 6200000 SAM alignment record pairs processed. 6300000 SAM alignment record pairs processed. 6400000 SAM alignment record pairs processed. 6500000 SAM alignment record pairs processed. 6600000 SAM alignment record pairs processed. 6700000 SAM alignment record pairs processed. 6800000 SAM alignment record pairs processed. 6900000 SAM alignment record pairs processed. 7000000 SAM alignment record pairs processed. 7100000 SAM alignment record pairs processed. 7200000 SAM alignment record pairs processed. 7300000 SAM alignment record pairs processed. 7400000 SAM alignment record pairs processed. 7500000 SAM alignment record pairs processed. 7600000 SAM alignment record pairs processed. 7700000 SAM alignment record pairs processed. 7800000 SAM alignment record pairs processed. 7900000 SAM alignment record pairs processed. 8000000 SAM alignment record pairs processed. 8100000 SAM alignment record pairs processed. 8200000 SAM alignment record pairs processed. 8300000 SAM alignment record pairs processed. 8400000 SAM alignment record pairs processed. 8500000 SAM alignment record pairs processed. 8600000 SAM alignment record pairs processed. 8616949 SAM alignment pairs processed. 1985 GFF lines processed. 100000 SAM alignment record pairs processed. 200000 SAM alignment record pairs processed. 300000 SAM alignment record pairs processed. 400000 SAM alignment record pairs processed. 500000 SAM alignment record pairs processed. 600000 SAM alignment record pairs processed. 700000 SAM alignment record pairs processed. 800000 SAM alignment record pairs processed. 900000 SAM alignment record pairs processed. 1000000 SAM alignment record pairs processed. 1100000 SAM alignment record pairs processed. 1200000 SAM alignment record pairs processed. 1300000 SAM alignment record pairs processed. 1400000 SAM alignment record pairs processed. 1500000 SAM alignment record pairs processed. 1600000 SAM alignment record pairs processed. 1700000 SAM alignment record pairs processed. 1800000 SAM alignment record pairs processed. 1900000 SAM alignment record pairs processed. 2000000 SAM alignment record pairs processed. 2100000 SAM alignment record pairs processed. 2200000 SAM alignment record pairs processed. 2300000 SAM alignment record pairs processed. 2400000 SAM alignment record pairs processed. 2500000 SAM alignment record pairs processed. 2600000 SAM alignment record pairs processed. 2700000 SAM alignment record pairs processed. 2800000 SAM alignment record pairs processed. 2900000 SAM alignment record pairs processed. 3000000 SAM alignment record pairs processed. 3100000 SAM alignment record pairs processed. 3200000 SAM alignment record pairs processed. 3300000 SAM alignment record pairs processed. 3357688 SAM alignment pairs processed. Loading required package: S4Vectors Loading required package: stats4 Loading required package: BiocGenerics Loading required package: parallel Attaching package: ‘BiocGenerics’ The following objects are masked from ‘package:parallel’: clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport, clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply, parSapply, parSapplyLB The following objects are masked from ‘package:stats’: IQR, mad, sd, var, xtabs The following objects are masked from ‘package:base’: anyDuplicated, append, as.data.frame, basename, cbind, colnames, dirname, do.call, duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply, union, unique, unsplit, which, which.max, which.min Attaching package: ‘S4Vectors’ The following object is masked from ‘package:base’: expand.grid Loading required package: IRanges Loading required package: GenomicRanges Loading required package: GenomeInfoDb Loading required package: SummarizedExperiment Loading required package: Biobase Welcome to Bioconductor Vignettes contain introductory material; view with 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")', and for packages 'citation("pkgname")'. Loading required package: DelayedArray Loading required package: matrixStats Attaching package: ‘matrixStats’ The following objects are masked from ‘package:Biobase’: anyMissing, rowMedians Loading required package: BiocParallel Attaching package: ‘DelayedArray’ The following objects are masked from ‘package:matrixStats’: colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges The following objects are masked from ‘package:base’: aperm, apply, rowsum estimating size factors estimating dispersions gene-wise dispersion estimates mean-dispersion relationship final dispersion estimates fitting model and testing null device 1 Loading required package: ggplot2 Loading required package: ggrepel null device 1 Attaching package: ‘dplyr’ The following object is masked from ‘package:matrixStats’: count The following object is masked from ‘package:Biobase’: combine The following objects are masked from ‘package:GenomicRanges’: intersect, setdiff, union The following object is masked from ‘package:GenomeInfoDb’: intersect The following objects are masked from ‘package:IRanges’: collapse, desc, intersect, setdiff, slice, union The following objects are masked from ‘package:S4Vectors’: first, intersect, rename, setdiff, setequal, union The following objects are masked from ‘package:BiocGenerics’: combine, intersect, setdiff, union The following objects are masked from ‘package:stats’: filter, lag The following objects are masked from ‘package:base’: intersect, setdiff, setequal, union Warning message: Column `id` joining factors with different levels, coercing to character vector [1] "fold_change" "id" "pvalue" "padj" [5] "log2FoldChange" "gff_annotation" Warning message: Column `ko` joining factors with different levels, coercing to character vector Warning message: Column `id` joining factor and character vector, coercing into character vector null device 1 null device 1 null device 1 null device 1 Attaching package: ‘plyr’ The following object is masked from ‘package:matrixStats’: count The following object is masked from ‘package:IRanges’: desc The following object is masked from ‘package:S4Vectors’: rename slurmstepd: error: Exceeded step memory limit at some point.