LOCUS       pKR147                 8186 bp    DNA     circular SYN 01-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(join(7951..8186,1..310))
                     /note="pLannotate"
                     /label="p15A ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(7562..7666)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      6354..6440
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(6701..7561)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      6532..6559
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             4841..5989
                     /note="pLannotate"
                     /label="xylR"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2333..2695)
                     /note="pLannotate"
                     /label="INC10_ECOLI"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1470..2372)
                     /note="pLannotate"
                     /label="insD"
                     /database="swissprot"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3511..4185
                     /note="pLannotate"
                     /label="REPX_STAAU"
                     /database="swissprot"
                     /identity="99.6"
                     /match_length="98.3"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1070..1456)
                     /note="pLannotate"
                     /label="cat (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="59.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    6120..6164
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="78.9"
                     /fragment="True"
                     /other="misc_feature"
     ncRNA           6235..6350
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     promoter        6578..6620
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="46.7"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(458..509)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      333..453
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="95.0"
                     /match_length="16.1"
                     /fragment="True"
                     /other="rep_origin"
     promoter        complement(6629..6648)
                     /note="pLannotate"
                     /label="tet promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="69.0"
                     /fragment="True"
                     /other="promoter"
     promoter        6032..6053
                     /note="pLannotate"
                     /label="T5 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="48.9"
                     /fragment="True"
                     /other="promoter"
     misc_feature    520..542
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.4"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      complement(7808..7863)
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="rep_origin"
     CDS             4393..4409
                     /note="pLannotate"
                     /label="MNase (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttatgcggcc gcttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
      481 cgcatctgtg cggtatttca caccgcatag gaagatccct cgacctgcag gcatgcaagc
      541 ttctgtaggt ttttaggcat aaaactatat gatttacccc taaatcttta aaatgcccct
      601 taaaattcaa aataaaggca tttaaaattt aaatatttct tgtgataaag tttgttaaaa
      661 aggagtggtt ttatgactgt tatgtggtta tcgattatag gtatgtggtt ttgtattgga
      721 atggcatttt ttgctatcaa ggttattaaa aataaaaatt agaccacgca tttatgccga
      781 gaaaatttat tgtgcgttga gaagaaccct taactaaact tgcagacgaa tgtcggcata
      841 gcgtgagcta ttaagccgac cattcgacaa gttttgggat tgttaagggt tccgaggctc
      901 aacgtcaata aagcaattgg aataaagaag cgaaaaagga gaagtcggtt cagaaaaaga
      961 aggatatgga tctggagctg taatataaaa accttcttca actaacgggg caggttagtg
     1021 acattagaaa accgactgta aaaagtacag tcggcattat ctcatattat aaaagccagt
     1081 cattaggcct atctgacaat tcctgaatag agttcataaa caatcctgca tgataaccat
     1141 cacaaacaga atgatgtacc tgtaaagata gcggtaaata tattgaatta cctttattaa
     1201 tgaattttcc tgctgtaata atgggtagaa ggtaattact attattattg atatttaagt
     1261 taaacccagt aaatgaagtc catggaataa tagaaagaga aaaagcattt tcaggtatag
     1321 gtgttttggg aaacaatttc cccgaaccat tatatttctc tacatcagaa aggtataaat
     1381 cataaaactc tttgaagtca ttctttacag gagtccaaat accagagaat gttttagata
     1441 caccatcaaa aattgtggat ttgcccctat atttccagac atctgttatc acttaaccca
     1501 ttacaagccc gctgccgcag atattcccgt ggcgagcgat aacccagcgc actatgcgga
     1561 tgccattcgt tataatgctc gaacgcctct gcaaggttct ttgctgccgt taacccgtct
     1621 ggtttgggca tgatactgat gtagtcacgc tttatcgttt tcacgaagct ctctgctatt
     1681 ccgttactct ccggactccg caccgccgtg ttcttcggtt caagtcccaa catccgggcg
     1741 aactggcgtg tttcattagc ccggtagcat gaaccattat ccgtcagcca ctccactgga
     1801 gacgacggaa gatcgttgcc gaagcggcgt tccaccgctc ccagcatgac gtcctgtact
     1861 gtttcactgt tgaagccgcc ggtagtgacc gcccagtgca gtgcctcacg atcacagcag
     1921 tccagcgcga acgtgacacg cagtctctct ccgttatcac agcagaactc gaacccgtca
     1981 gagcaccatc gctgattgct ttctttcacg gccactctgc ctgtatgtgc ccgtttcgat
     2041 ggcggtacag caggttttcg ctcaagcaac agcgcattct ggcgcatgat ccggtaaaca
     2101 cgtttggcat tgatcgcagg cataccatca agttctgcct gtctgcgaag cagcgcccat
     2161 acccgacgat aaccatacgt tggcagctct ccgataacat ggtgtatacg gagaagcaca
     2221 tccgtatcat cagtgtgacg actgcggcgg ccatccatcc agtcatcggt tcgtctgaga
     2281 atgacgtgca actgcgcacg cgacacccgg agacaacggc tgactaagct tactccccat
     2341 ccccgggcaa taagggcgcg tgcgctatcc acttttttgc ccgtccatat tcaacggctt
     2401 ctttgaggag ttcattttcc atcgttttct tgccgagcag gcgctggagt tctttaatct
     2461 gcttcatggc ggcagcaagt tcagaggcag gaacaacctg ttctccggcg gcgacagcag
     2521 taagacttcc ttcctggtat tgcttacgcc agagaaataa ctggctggct gctacaccat
     2581 gttgccgggc aacgagggag accgtcatcc ccggttcaaa gctctgctga acaattgcga
     2641 tcttttcctg tgtggtacgc cgtctgcgtt tctccggccc taagacatca atcatctgtt
     2701 ctccaatgac tagtctaaaa actagtatta agactatcac ttatttaagt gatattggtt
     2761 gtctggagat tcagggggcc agtctaaatt gtataaagtg gctctaactt atcccaataa
     2821 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
     2881 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
     2941 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
     3001 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
     3061 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
     3121 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     3181 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga
     3241 ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc
     3301 cttttctttc ttatcttgat aataagggta actattgccg gcgaggctag ttacccttaa
     3361 gttattggta tgactggttt taagcgcaaa aaaagttgct ttttcgtacc tattaatgta
     3421 tcgttttaaa tgaatagtaa aaaacataca tagaaagggg aaaaagcaac tttttttatt
     3481 gtcatagttt gtgaaaacta agttgttttt atgtgttata acatggaaaa gtatactgag
     3541 aaaaaacaaa gaaatcaagt atttcagaaa tttattaaac gtcatattgg agagaatcaa
     3601 atggatttag ttgaagattg caatacattt ctgtcttttg tagctgataa aactttagaa
     3661 aaacagaaat tatataaagc taattcttgt aaaaatcgat tttgtcctgt ctgtgcttgg
     3721 agaaaagcta gaaaagatgc attgggttta tctttgatga tgcaatatat taagcagcaa
     3781 gagaaaaagg agtttatctt tttaactttg actacaccta atgtaatgag tgatgaatta
     3841 gaaaatgaaa taaaacgtta taataattct tttagaaaac ttataaagag aaaaaaagta
     3901 ggtagtgtta taaagggata tgttcgtaag ttagagatta catataataa aaaaagagat
     3961 gattataatc ctcattttca tgtgttaatt gcagtaaata aatcgtattt cacagataaa
     4021 agatattata ttagccaaca agaatggtta gatttatggc gtgatgtaac gggcatttca
     4081 gaaataacac aagttcaagt tcaaaaaata agacaaaata ataataaaga attatatgaa
     4141 atggctaagt attctggtaa agatagtgat tatttaataa atcaaaaagt ctttgatgca
     4201 ttttataaat cacttaaagg taaacaggta ttagtttatt caggattatt taaagaggct
     4261 aaaaagaaat taaaaaatgg ggatttagat tacttaaaag aaattgatcc aaccgaatat
     4321 atctatcaaa ttttttatat ttggaaacaa aaagagtatt tagctagtga actttatgac
     4381 ttaacagaac aagaaaaaag agaaattaat cacaaaatga tagacgaaat cgaggaagaa
     4441 caataacaaa atataagtgc taacagtcgt ctgcaagttt agttaagggt tcttctcaac
     4501 gcacaataaa ttttctcggc ataaatgcgt ggtctaattt ttatttttaa taaccttgat
     4561 agcaaaaaat gccattccaa tacaaaacca catacctata atcgataacc acataacagt
     4621 cataaaacca ctccttttta acaaacttta tcacaagaaa tattttggca ttctacgact
     4681 ataacttaaa tttatatttt ttactttata atatataatt gattatagaa taatgttgct
     4741 catatcgttt gccaacatct agtactcaaa ttacactatg ttacacttgg taatattaac
     4801 cgaacttccc ctgtccaaat tagataagag gtaataataa atggaaaata attttatagt
     4861 aaatgaaaat gagaagcgtg tattaaaaca aattttcaat aacagcaata tttcacgaac
     4921 acaaatatcg aagaatttag aacttaataa agctactatt tctaacattc tgaacaactt
     4981 aaaacacaag agtttagtta atgaagtagg agaaggtaat agtactaaaa gtggtggacg
     5041 aaagcctatt ttactcgaaa ttaaccaaaa atatggctac tatatttcta tggatttaac
     5101 atatgattcc gttgaattaa tgtacaacta ctttgatgct actatattaa agcaagattc
     5161 ctacgaatta aatgataaaa atgtaagcag tatattacaa attttaaaat ctaatataaa
     5221 cgtctcagaa aaatatgata cgttatatgg gttacttggt atatctatat ccatacacgg
     5281 tatcgttgac gatgagcaaa acataatcaa tcttcctttt cataaaaatg agaaacgcac
     5341 atttaccgat gaattaaagt cattcacaaa tgttcctgtc gttatagaaa atgaagcaaa
     5401 tttatcagcg ctatatgaaa aaagtttata tattaattca aacataaata atttgattac
     5461 tttaagtatt cacaagggta taggcgctgg catcctaata aataaaaaac tttatcgtgg
     5521 ctcaaatgga gaggctggag agataggtaa gacattggtt ttggaatcta taaataacaa
     5581 tgacaacaaa tattataaaa tcgaagatat atgctcccaa gacgctttaa tacagaaaat
     5641 aaataatagg ttgggcgtca cattgacgtt tacagaacta atccaatatt acaacgaagg
     5701 aaattcaatt gttgctcatg aaattaaaca atttattaat aaaatgacag ttctgattca
     5761 taatttgaat acacaattta acccagacgc tatttatatt aactgtcctt taattaatga
     5821 attaccaaat attttaaatg aaattaaaga gcaattctcc tgtttttctc aaggcagtcc
     5881 agttcaatta catttaacta ctaatgtaaa acaagctact ttattgggtg gcactttagc
     5941 aataatgcaa aaaacattaa atataaataa cattcaaatg aatattaaat aattacagca
     6001 gtctgagtta taaaatagat atctcggacc gtcataaaaa atttatttgc tttcaggaaa
     6061 atttttctgt ataatagatt caagttagtt tgtttattaa attaaccaac taaaatgtag
     6121 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcagccaagc ttgggctttt
     6181 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     6241 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     6301 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     6361 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     6421 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     6481 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
     6541 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
     6601 tcaaatatgt atccgctcat ccccatccta tcgatgataa gctgtcaaac atgagaatta
     6661 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6721 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6781 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6841 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6901 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6961 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7021 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7081 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7141 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7201 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7261 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     7321 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgctctt
     7381 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7441 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7501 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7561 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7621 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7681 aagtgccacc tggcggccgc actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     7741 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     7801 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     7861 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     7921 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     7981 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     8041 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     8101 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     8161 aggcagttcg ctccaagctg gactgt
//
