LOCUS       pKR147                 6850 bp    DNA     circular SYN 01-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(join(6615..6850,1..310))
                     /note="pLannotate"
                     /label="p15A ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(6226..6330)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      5018..5104
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(5365..6225)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1070..1717)
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      5196..5223
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             3505..4653
                     /note="pLannotate"
                     /label="xylR"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2175..2849
                     /note="pLannotate"
                     /label="REPX_STAAU"
                     /database="swissprot"
                     /identity="99.6"
                     /match_length="98.3"
                     /fragment="False"
                     /other="CDS"
     misc_feature    4784..4828
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="78.9"
                     /fragment="True"
                     /other="misc_feature"
     ncRNA           4899..5014
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     promoter        5242..5284
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="46.7"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(458..509)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      333..453
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="95.0"
                     /match_length="16.1"
                     /fragment="True"
                     /other="rep_origin"
     promoter        complement(5293..5312)
                     /note="pLannotate"
                     /label="tet promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="69.0"
                     /fragment="True"
                     /other="promoter"
     promoter        4696..4717
                     /note="pLannotate"
                     /label="T5 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="48.9"
                     /fragment="True"
                     /other="promoter"
     misc_feature    520..542
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.4"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      complement(6472..6527)
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="rep_origin"
     CDS             3057..3073
                     /note="pLannotate"
                     /label="MNase (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttatgcggcc gcttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
      481 cgcatctgtg cggtatttca caccgcatag gaagatccct cgacctgcag gcatgcaagc
      541 ttctgtaggt ttttaggcat aaaactatat gatttacccc taaatcttta aaatgcccct
      601 taaaattcaa aataaaggca tttaaaattt aaatatttct tgtgataaag tttgttaaaa
      661 aggagtggtt ttatgactgt tatgtggtta tcgattatag gtatgtggtt ttgtattgga
      721 atggcatttt ttgctatcaa ggttattaaa aataaaaatt agaccacgca tttatgccga
      781 gaaaatttat tgtgcgttga gaagaaccct taactaaact tgcagacgaa tgtcggcata
      841 gcgtgagcta ttaagccgac cattcgacaa gttttgggat tgttaagggt tccgaggctc
      901 aacgtcaata aagcaattgg aataaagaag cgaaaaagga gaagtcggtt cagaaaaaga
      961 aggatatgga tctggagctg taatataaaa accttcttca actaacgggg caggttagtg
     1021 acattagaaa accgactgta aaaagtacag tcggcattat ctcatattat aaaagccagt
     1081 cattaggcct atctgacaat tcctgaatag agttcataaa caatcctgca tgataaccat
     1141 cacaaacaga atgatgtacc tgtaaagata gcggtaaata tattgaatta cctttattaa
     1201 tgaattttcc tgctgtaata atgggtagaa ggtaattact attattattg atatttaagt
     1261 taaacccagt aaatgaagtc catggaataa tagaaagaga aaaagcattt tcaggtatag
     1321 gtgttttggg aaacaatttc cccgaaccat tatatttctc tacatcagaa aggtataaat
     1381 cataaaactc tttgaagtca ttctttacag gagtccaaat accagagaat gttttagata
     1441 caccatcaaa aattgtataa agtggctcta acttatccca ataacctaac tctccgtcgc
     1501 tattgtaacc agttctaaaa gctgtatttg agtttatcac ccttgtcact aagaaaataa
     1561 atgcagggta aaatttatat ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa
     1621 tttctgtggt tatactaaaa gtcgtttgtt ggttcaaata atgattaaat atctcttttc
     1681 tcttccaatt gtctaaatca attttattaa agttcatttg atatgcctcc taaattttta
     1741 tctaaagtga atttaggagg cttacttgtc tgctttcttc attagaatca atcctttttt
     1801 aaaagtcaat attactgtaa cataaatata tattttaaaa atatcccact ttatccaatt
     1861 ttcgtttgtt gaactaatgg gtgctttagt tgaagaataa aagaccacat taaaaaatgt
     1921 ggtcttttgt gtttttttaa aggatttgag cgtagcgaaa aatccttttc tttcttatct
     1981 tgataataag ggtaactatt gccggcgagg ctagttaccc ttaagttatt ggtatgactg
     2041 gttttaagcg caaaaaaagt tgctttttcg tacctattaa tgtatcgttt taaatgaata
     2101 gtaaaaaaca tacatagaaa ggggaaaaag caactttttt tattgtcata gtttgtgaaa
     2161 actaagttgt ttttatgtgt tataacatgg aaaagtatac tgagaaaaaa caaagaaatc
     2221 aagtatttca gaaatttatt aaacgtcata ttggagagaa tcaaatggat ttagttgaag
     2281 attgcaatac atttctgtct tttgtagctg ataaaacttt agaaaaacag aaattatata
     2341 aagctaattc ttgtaaaaat cgattttgtc ctgtctgtgc ttggagaaaa gctagaaaag
     2401 atgcattggg tttatctttg atgatgcaat atattaagca gcaagagaaa aaggagttta
     2461 tctttttaac tttgactaca cctaatgtaa tgagtgatga attagaaaat gaaataaaac
     2521 gttataataa ttcttttaga aaacttataa agagaaaaaa agtaggtagt gttataaagg
     2581 gatatgttcg taagttagag attacatata ataaaaaaag agatgattat aatcctcatt
     2641 ttcatgtgtt aattgcagta aataaatcgt atttcacaga taaaagatat tatattagcc
     2701 aacaagaatg gttagattta tggcgtgatg taacgggcat ttcagaaata acacaagttc
     2761 aagttcaaaa aataagacaa aataataata aagaattata tgaaatggct aagtattctg
     2821 gtaaagatag tgattattta ataaatcaaa aagtctttga tgcattttat aaatcactta
     2881 aaggtaaaca ggtattagtt tattcaggat tatttaaaga ggctaaaaag aaattaaaaa
     2941 atggggattt agattactta aaagaaattg atccaaccga atatatctat caaatttttt
     3001 atatttggaa acaaaaagag tatttagcta gtgaacttta tgacttaaca gaacaagaaa
     3061 aaagagaaat taatcacaaa atgatagacg aaatcgagga agaacaataa caaaatataa
     3121 gtgctaacag tcgtctgcaa gtttagttaa gggttcttct caacgcacaa taaattttct
     3181 cggcataaat gcgtggtcta atttttattt ttaataacct tgatagcaaa aaatgccatt
     3241 ccaatacaaa accacatacc tataatcgat aaccacataa cagtcataaa accactcctt
     3301 tttaacaaac tttatcacaa gaaatatttt ggcattctac gactataact taaatttata
     3361 ttttttactt tataatatat aattgattat agaataatgt tgctcatatc gtttgccaac
     3421 atctagtact caaattacac tatgttacac ttggtaatat taaccgaact tcccctgtcc
     3481 aaattagata agaggtaata ataaatggaa aataatttta tagtaaatga aaatgagaag
     3541 cgtgtattaa aacaaatttt caataacagc aatatttcac gaacacaaat atcgaagaat
     3601 ttagaactta ataaagctac tatttctaac attctgaaca acttaaaaca caagagttta
     3661 gttaatgaag taggagaagg taatagtact aaaagtggtg gacgaaagcc tattttactc
     3721 gaaattaacc aaaaatatgg ctactatatt tctatggatt taacatatga ttccgttgaa
     3781 ttaatgtaca actactttga tgctactata ttaaagcaag attcctacga attaaatgat
     3841 aaaaatgtaa gcagtatatt acaaatttta aaatctaata taaacgtctc agaaaaatat
     3901 gatacgttat atgggttact tggtatatct atatccatac acggtatcgt tgacgatgag
     3961 caaaacataa tcaatcttcc ttttcataaa aatgagaaac gcacatttac cgatgaatta
     4021 aagtcattca caaatgttcc tgtcgttata gaaaatgaag caaatttatc agcgctatat
     4081 gaaaaaagtt tatatattaa ttcaaacata aataatttga ttactttaag tattcacaag
     4141 ggtataggcg ctggcatcct aataaataaa aaactttatc gtggctcaaa tggagaggct
     4201 ggagagatag gtaagacatt ggttttggaa tctataaata acaatgacaa caaatattat
     4261 aaaatcgaag atatatgctc ccaagacgct ttaatacaga aaataaataa taggttgggc
     4321 gtcacattga cgtttacaga actaatccaa tattacaacg aaggaaattc aattgttgct
     4381 catgaaatta aacaatttat taataaaatg acagttctga ttcataattt gaatacacaa
     4441 tttaacccag acgctattta tattaactgt cctttaatta atgaattacc aaatatttta
     4501 aatgaaatta aagagcaatt ctcctgtttt tctcaaggca gtccagttca attacattta
     4561 actactaatg taaaacaagc tactttattg ggtggcactt tagcaataat gcaaaaaaca
     4621 ttaaatataa ataacattca aatgaatatt aaataattac agcagtctga gttataaaat
     4681 agatatctcg gaccgtcata aaaaatttat ttgctttcag gaaaattttt ctgtataata
     4741 gattcaagtt agtttgttta ttaaattaac caactaaaat gtagaattcg agctcggtac
     4801 ccggggatcc tctagagtcg acctgcagcc aagcttgggc ttttcagcct gatacagatt
     4861 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     4921 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     4981 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     5041 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     5101 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     5161 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     5221 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
     5281 tcatccccat cctatcgatg ataagctgtc aaacatgaga attaaatcaa tctaaagtat
     5341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     6001 acatagcaga actttaaaag tgctcatcat tggaaaacgc tcttcggggc gaaaactctc
     6061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctggcgg
     6361 ccgcactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     6421 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     6481 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     6541 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     6601 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     6661 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     6721 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     6781 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     6841 gctggactgt
//
