	   SnapGene      atatGGATCCttatgtctaaatactataacctcttcttttcataaaaaattatacttattaaaatctacctgattatttacttattttgataaatatagttaaggtcaagagtcataaattgattataatattatcgagctttatcataccgaataattacgatgtcaatgaaacaaaaatttattatttatttaccagctttatttataatttgttttatcactacaatatatatttactgtaataatggaatagcatatcaagagataatgttagatacttactaacagcaatagctataatatatggatttaatataaatgctacaatgaaaattaattcctcaagagaatcctctagtgacgaatccaaatatttaaaatacttagtgtctttagcaaatattattacattccttgtattttttatgtgtttagtaatcttattatctacaaatgtagaagtatctcatcttacagcttttacaacttgttttttaagtattttaagtgctggttttgtaccaacacttttgataatactaagagattattaaaagaaaacagctacttgattatcaacttcaaaagttgtgatagctttatgacttgaaTTAAGCATAATCTGGAACATCGTATGGGTATGCGGCCGCatat    GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               	   	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    	 	                     !s{   O  W  xi XpW t  Ga m ^mh m h^m I& 	  '5Ve   '5V   ( 2 ^ q |      */1<[k|D  q/ u    NRd Vb: c 8Py)f C f   '5V   7zXZ  ִF !   t/^] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!ǚ^!8?>H=}tLg>.r_*_F?]bAaV$ 	|7,)W[=mV2Vd>},{Ϥ.G=ϥBT#bx+8;+k+ųݪ/!bĸ& ?/fd>f_b+j%r[/4
) Z;![)"4۽TJf [^gbP7GܾשJ~N:ciu5~hAQ.A]RfBזBh˿3:ޕO=KH쒕=,g\%w _`(Tg)HD M9	{W[	(2Г5ڰK=kA@ug}GbDcIy$fq+
6E0D.L71n{$1|lQ}ya˺L5LMJt#3CھXk!YR1Юqr|p|!ƍ%sHo9G+]GBgIeV	eA"QWx
]I([󎐇+NU<.mͶN>,(4ǔrLzВ>Ec=4630r>t<uGg=.Bum5"
~*9881a$.a|V4Ipɿ aÊ6G0Sx4H!+8}	|(7@K>5}fD    kR_	   ug    YZ   -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="2"><Feature recentID="0" name="bS21-2-HA" directionality="2" translationMW="1102.17" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="615-644" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA*"/></Q></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  _<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P584 Dnmr1_FR2_F" sequence="ATATGCGGCCGCatatttccaactattcataatctgtgag" description="Contains NotI site"><BindingSite location="642-656" boundStrand="1" annealedBases="ATATGCGGCCGCata" meltingTemperature="53"><Component hybridizedRange="642-656" bases="ATATGCGGCCGCata"/><Component bases="tttccaactattcataatctgtgag"/></BindingSite><BindingSite simplified="1" location="642-656" boundStrand="1" annealedBases="ATATGCGGCCGCata" meltingTemperature="53"><Component hybridizedRange="642-656" bases="ATATGCGGCCGCata"/><Component bases="tttccaactattcataatctgtgag"/></BindingSite></Primer><Primer recentID="1" name="KROL687 rpsU2_HA_FR2_F" sequence="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-02T17:37:03Z"><BindingSite location="612-656" boundStrand="1" annealedBases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAtt" meltingTemperature="69"><Component hybridizedRange="612-656" bases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAtt"/><Component bases="aaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="612-656" boundStrand="1" annealedBases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAtt" meltingTemperature="69"><Component hybridizedRange="612-656" bases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAtt"/><Component bases="aaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL692 rpsU3_HA_FR2_R" sequence="atatGGATCCttatgtctaaatactataacctcttcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-04T18:31:38Z"><BindingSite location="0-38" boundStrand="0" annealedBases="atatGGATCCttatgtctaaatactataacctcttcttt" meltingTemperature="58"><Component hybridizedRange="0-38" bases="atatGGATCCttatgtctaaatactataacctcttcttt"/></BindingSite><BindingSite simplified="1" location="0-38" boundStrand="0" annealedBases="atatGGATCCttatgtctaaatactataacctcttcttt" meltingTemperature="58"><Component hybridizedRange="0-38" bases="atatGGATCCttatgtctaaatactataacctcttcttt"/></BindingSite></Primer><Primer recentID="3" name="KROL691 rpsU3_HA_FR2_F" sequence="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttcaagtcataaagctatcacaactttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-04-04T18:31:37Z"><BindingSite location="586-656" boundStrand="1" annealedBases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttcaagtcataaagctatcacaactttt" meltingTemperature="71"><Component hybridizedRange="586-656" bases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttcaagtcataaagctatcacaactttt"/></BindingSite><BindingSite simplified="1" location="586-656" boundStrand="1" annealedBases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttcaagtcataaagctatcacaactttt" meltingTemperature="71"><Component hybridizedRange="586-656" bases="atatGCGGCCGCATACCCATACGATGTTCCAGATTATGCTTAAttcaagtcataaagctatcacaactttt"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>6483c371-3dfe-4efc-bfed-c7de19414c5f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="18:31:41">2024.4.4</Created>
<LastModified UTC="18:31:41">2024.4.4</LastModified>
<CreatedBy>Trial User</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         6+ Cutters                                                                                                  d     CHO  d               E             Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
