	   SnapGene      
GCTAGTGGTGCTAGCCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGGGATCCcaattgCAGCTGATaatATGTCGACGGTACCGCTCTTCTTAATACGACTCACTATAGGgagtatatgtgaatatttaaaaataggagtatctatatgaaaaaaataattaagcttagtcttttatctttatcaatcgcaggtttagcgagctgttctactctagggttaggtggctctgatgatgcaaaagcttcagctaaagatactgctgctgctcagacagctactactgagcaagctgctgctgtatctaagccaactgcaaaagtaagtttaaataaacttggtcaggataaaataaaagcaactgtatatacagcatacaataataacccacaaggaagtgtaagattacaatggcaggctccagaaggttctaagtgccatgatacaagcttcccaattactaagtatgctgagaagaacgataaaacttgggcaactgtaacagttaagcaaggtaataacttctgtagcggtaagtggacagctaatgtagtttatgacaaagaagtaatcgcttctgattcaataaatatttaatttcttcaaatatttataattttctagtttttgcttttctcaaaaaaaaaCCCGGGCCATGGCTCGAGGCTAGCGGTCCCGGGGGATCGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAAGCTTGGCACTGGCCGACCGGGGTCGAGCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGCTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACAGATCCGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGT     
   
   	                  
               	                                                                  	   	                                    
   
      
                                                                                                      4                                    
   	      	               	                                    	            
                                    	   
            	         
      	   
                           
            	   
   
      	                     
            
                                 
            
      
      
               
         
                                                	   	   	               
      
            
   
      	   
   	                        	               	   	         	         
              
 2 !Wi	

 > } _i	6
	 3  "Xj
~

 c	>
 ~  2` 5Alz	
7
G
 ? hrIj		
 *N
   =D 	
 ^ #,kW 4   #l

 cYrk @_|H	^		?
	 I^<#
  zd\ * Op ( 3;a 9		   ;#	O	  -]{

 DBmR  
q
  .CA		H


  ;	8
P

 6{	   		 P iXQZk	
 Ss6 @ JU]!v			 [ .p  +>JKB
 r dOPA	  SK	   (>xB  	!	
  LE@		  xw			 5N Q$G-l			   <{XB	
 _ %	 :*-Q		M
 5  
 #  U8$b (S

  v/m		
 X ZsIP d7G1	a	  6l&
 =
 !~F	

  A}}I		@
  `_	

 lz!<#t	
 J  _bx03>~	  /Ku,|%
$
.
  =	t
 J_	
 M  369+i{y	   	 : 	$/F6  MEe]	 d  n1p|sLgT		 P+
  DXe0	v	 #= + qZ	 1 = |   b5
=
}

  g14MqH	
   < ) ^;{"
 ' :z C@Q:		
 W5TP		  J  'wv?		   PF$	 T7~9P		  6<0	   |

 .t^aw/2}		
s
 L   #2.Dof  Cd 0 0Gz	!
4
<
  BVIPS	 OS&E5/		
  1BsCn.r
  NrA/UF	3

4           Mlq'(BCDE			

&
0
1
2



   Ym[l)'
I


  ]D		

 2~(
J

 S m    ZnX\m<	d	n	9
Q
h


 F  cv  I*	 |'s	 n

 G^w7/T			  E	A+		 Z
   <
	 V  3	K

 8  		D	)
	 z  
7=	{	
 Q  [joYtSRl	:

 T    }]n;i

 n Y[e	o	R
X

 G 3	n

	  t7k
  _dpw*v



 Tb4	  J+3U?  "
w	z
   >K^"l			 A =( V3		 \ g]g	E
 p N}Xt (	 /:qJ !,Ko9
	 ?%	
 y	

 LC
 *HxLa[h		
 w '8P0U _Q		 s  eB'			T
Z
 a TL	q		 [i,	
    F
B		 

 )ky	  bjCV	  HN?]o[ ,:"	8		

	   -O
p


 O   R\	 =co@	  	 ;z4MA		
	  ',	L
 W %F
 k}y "E	
 I  J+{	  9 58*x5	S			 </	Y	*

 < {    
 & O
   6}8>	   v|	  AR
%H
	;
  X\kpZT	
 R l  1Hbum	 .mS	 7 U   ;YC	
 y   |~s:R  ^o)j
m



    =<'2Ty
 o 9fW\f		 8

  &)&		S
Y

 ` Zhp		 a	GU		 9[	N
o

 &+
 H )*;!.4R	X	 %    u
 k   W	a	 6   :r&Sl
x

 8g		
 (%)Z T
 $     V9`q		w

  x%&'!"#$a		b
c
 eycb+	 

d


 w,		 Ui0_c	
e
 h  Ofzc	5		
 dn^


 px	 Y [,	 tv4-J@NQ	  KgV e8ud	{
   Vj3Nx)	b		f
 1` 	2			V


 u #H
  Pm#m	6	
    ?{d' f i   g7_V					\

 ~L	
 >)i	

 >4	 W_

 B erDo
 Vh^h		 @F

 ] "q+k yG	]	
  \~~N	 B~	
  Yu	
 Z q R-IOJ	A
 wKA	 
	 " ua.)R		
 5HO`	 ;s	
  =$-

 c Lhm0{ErK	 "Wu	  fL]p:
|
 9y4?	 vu	 K "-`c1e	
  / 04-Oy.		   L@k&

%
/

 E  W}c	g
	 Fv@&r*			 	
	  	6z	W

 2a3		u

 !2k>			 MID
 $x	

 v K`Zg	 	

 Mj\in	
 N  +Q,bn?7		  :y $		 47Ij|z
  
 ;  |
 
@0G x  9Q1(	  %(eV7
 `F8W		 j  %Rf	   Y`				a
 g Nh^		]
 fouM	  t 2Mt(			U

 e   }U		[
 qCh ?U*j\		 QHM	
 ! G		 b Dr		,

 \t	
  . D E? q-			 5j1		 Yfw		 i> 	 $X`
  t   GCF[	

 - C sp		  hs  , fgEr
  $  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                     	                                               	                        	                     
         
   
            	   3   '      
   "   $               !                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                     	                 	        	       
   
 
    	 3 '  
 " $     !   Dw  D  D  D  F  H  Ko.! A/c	J		S~    3P AP AP A>D
{	~ d ) R	rX >" ^ $	^ b 
'	N	^	]				 	? u	
sx 7* ? ^ pl
8 2M	. Uh>)i|2RqJ	]		
. e [
	  /,? IT		 V		`147X  CX (	 e>7N
E >K 	K X  28M	 \mh
K	p$	 ? I	@ U	UiN
e
o

\i
J 36i{_b0y* pU[	 	

J _b036i{yj	J 36i{~_b0yF	+
U\i[	 =	 	L[h	t	2	t+7	\ x +@Gt7	vuK`Zg.p_	Cr Sd;AZ -HO P8m6	 
pg-lG		Rf g		\
;r dAG	S~F	+
K`Zgvu 0_E
	;s	
b Dr		,

!~F	
_ %	L   #2.Doflz!<#t	
 
 ;  |@ JU]!v			* Op?U*j\		
 * L    #2C.DOzoddp\f7 U y     |~;s :	R6Y	C	z	W


(	I  J+{	?U\i*>tj\		
 f?MUL]j*\ipn:j	\	

|
F  cv t( 3;ak   W	a	L     #2.D<o(f	Y [,	          
         
9 O{y.Ee*^نF̦`Uc*W5ߥe!g=5ViK_pq%R2 '[`,ʘ]+L^&͛H1ڿOl&ůqt採L0oZIBAiB lo9uOAN=^M>p.7,M;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ.9Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^jFUAߑZUaJmnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7j  7zXZ  ִF !   t/6] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@PpS- 
bC+ h'it
^'
h&S.E.٢/ZǼ9Apx0|Н4	OU?kzr-D2Jy~P>vk237;,2Ox1ĚV¿fGU~HTC(ܓbt+ڪgA%R..dΫٹa%w{G.ɽ1MVC<޵){χ~JG,
1k0S L(6Uu@'q};d{@R/KvRr-0of,ðm&@)nyd"9xGpk<sN$~Ifcy!6	_^-SA[^{@<6>.@QoϬ_|u)@/~&r?H=tFF@W2ƃ^*Ūˮ¨T@7;k&?nԘI륺+_# [H`*DhIfD&SvcfDBc"j]=45.*"Y%܀d9ߛ7W41+6-<:*40{:?_Zrd-Z\0KLƜm{8}MM,&9H뢜L<ӌ>i{Lfq_Fx_qU0Ȗ =3RK prɫQՉ-%1]ܪkEm{>ς?{-@Iϑ8-s'#W¯T*zsv{4 {΀܋7SP$<D;CMD8sGR&c>HP:+Z'׾4v$j?GZN	l:;G@efJX(ТS)9Hw9!rW	7U_QA p&k.a0xQ.Jǫ {.s]+H4
Xy*[V p XM3Xdon?OKވ#WS5Hޔ΀[|)Rot
F*('
6)l=Ţį!Z*Z85_̽ekX[gܯIɷG^^n`O9ME{	6yrjqBH,)3`̥dC    # (  %g    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_90l   D   I   R   T   ~    7zXZ  ִF !   t/t] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ8hlFOlY.k(DW`!
 	KJ9rEPܤ$FcprMUq3+Xfg=Gj6F#5Ɇca>48r7Nn2h݊r3u>ɧ&[_ 9o)b`.5·QhxOAd)9"qOG`yPlqsLu	j3^񋘢UnMM]=*a/aY P Vc(8Z/tMA-3"Ietih7eewH2Uj" V򋷹bg*xV<e﷍iYL bw&r,<K?G-#3iU
)K WqijcR.Z-уVtڡgۖXj%üx"]|pF+epbm<Ө7#<=5­ְ~zAw7pᙇ⭷5-oq2iM:%9*ݬOqcX8rjhWҟGs\/&Gݡ/x:lSQƈS7ĕӟrz<3V(9yiVpjfIWZॗ-KǗܹ~(M*wixɻ41Goy6@QAp>WeFzʅy[fHCtFXEV_[yuG"|+`V\q0Nw
z>Fg%jlzae.hSUɃBGR+nDsL'wmc}u)4sgT_|~tr1#K$$XX֝\!ɲLr.rw
Oإ(<ɞ!M	/	`P2&jμNi8y{N.-o]7bXY]"'Db.XdZX$Uۋo{giOE|kXF}hsdkcuKJ.a%J=}'l~z+{PUX +jrzU -	5" Ա80wU˨ePvZ4};yr{p{%
%[uctIU#(W	^W?_0x{/]w`[MIB LIolY.W5R]3"$p@^$!?& )ʲa:lê>$dp2d]$Sfv{y7-BkV  /8 /  qWg    YZ                  9=UGC@)@o֍8e|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    D   I   R   T   ~    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="81-87" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="15-62" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="108-126" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-125" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-612" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="163-612" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="163-612" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-253" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="314-334" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-127" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-662" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="233-252" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="233-252" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="313-333" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="313-333" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:54">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4b	gdYUIUVZSmlЩ18{);NFxӞxMN8nS׍TY^eYT׉gYeF`E
#gS֞RcQqz^%Ҕ%$RNXQIl놵fZJUU;   ;   @   I   K   u    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-78" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="99-117" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="116-116" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-603" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="154-603" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="154-603" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="225-244" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="305-325" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="118-118" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-653" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="224-243" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="224-243" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="304-324" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="304-324" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:47:10">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᙋ'륜{yf!fVA&aUYiNꛩB@ cU:ӕ;GNy58㉹O^5RRezAeS^&gMePR%uC+aNZyIEUxJSP^	H9bJuE%VjZ՚j*UU`C$   ;   @   I   J   t    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-77" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-116" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-602" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-602" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="153-602" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="224-243" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="304-324" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="117-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-652" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="223-242" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="223-242" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="303-323" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="303-323" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:33">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4ᘱ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   r    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="10" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-75" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="96-114" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-113" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-600" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="151-600" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="151-600" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="222-241" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="302-322" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="115-115" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-650" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="221-240" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="221-240" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="301-321" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="301-321" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:28">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Uʑ 9Z4|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   n    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  X<?xml version="1.0"?><Features recycledIDs="10,11,12,13,14,15,16,17,18,19,20" nextValidID="21"><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-110" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-109" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-596" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-596" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="147-596" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="218-237" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="298-318" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="111-111" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="597-646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,2,1,0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="217-236" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="217-236" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="297-317" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="297-317" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:46:14">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  13     Q          Uʑ 9Z4JHVYaUjUgꩶTZjzbފj*E($4Ji{Yjߩ%Qj֔e-ehijbD%&aaeھZ&QOdI}VƄ냦ZQje:][)}TzzAJFe؋G&MjfrީiJ(IVY:fS>U*.JQ%IEDnվ$Z&m:th`a&[H)RݴvU*i4ednjqKinud=mYVM;RUVU^$VV: ɪiEUTf۔ꚕ9F
/T -h{Ev	YkTY*=`UnUNmYe
dKmT<떪aR%)f(gUJjC%4flCNZ*yJIGmj@NZWJZZ1.JYeknb[sYj>Rnj(IibZYfNijM^EBڙzJ^Zb虩[Jf9v^ɤ|YǷ&bjUdnfU.[4*LN+
B5S9Q8tS8U%*VWVU5ftYu"QX4;BH\e^׉t5	e0Ӗ$TR[:efY:U_9    ;   @   G  
/  
j    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2608" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="FTL_0126" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="hypothetical protein" directionality="2" translationMW="95341.69" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="122-2584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2608-2608" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="16" name="pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-2584" color="#a6acb3" type="standard"/></Feature><Feature recentID="15" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2609-2627" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2610-2610" color="#a6acb3" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="FTL_0125" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="hypothetical protein" directionality="2" translationMW="1785.21" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="28" maxFusedRunOn="28"><Segment range="72-117" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="pdpB" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-117" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-53" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2666" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2665-2665" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-3152" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2703-3152" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="1" translationMW="15772.37" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2703-3152" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2774-2793" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="2" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2854-2874" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="1" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2667-2667" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3202" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="2574-2602" boundStrand="0" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="2574-2602" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="1" name="KROL311  pdpA_qPCR_R1" sequence="cacttgatctttgtatcgcactatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="0" annealedBases="cacttgatctttgtatcgcactatc" meltingTemperature="57"><Component hybridizedRange="2133-2157" bases="cacttgatctttgtatcgcactatc"/></BindingSite></Primer><Primer recentID="2" name="KROL310  pdpA_qPCR_F1" sequence="cttgagctccctcatgattaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite><BindingSite simplified="1" location="2190-2211" boundStrand="1" annealedBases="cttgagctccctcatgattaca" meltingTemperature="57"><Component hybridizedRange="2190-2211" bases="cttgagctccctcatgattaca"/></BindingSite></Primer><Primer recentID="3" name="KROL312  pdpA_qPCR_F2" sequence="aatcatgagggagctcaagaatta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite><BindingSite simplified="1" location="2193-2216" boundStrand="0" annealedBases="aatcatgagggagctcaagaatta" meltingTemperature="55"><Component hybridizedRange="2193-2216" bases="aatcatgagggagctcaagaatta"/></BindingSite></Primer><Primer recentID="4" name="KROL313  pdpA_qPCR_R2" sequence="cgacaaactataaagactct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite><BindingSite simplified="1" location="2269-2288" boundStrand="1" annealedBases="cgacaaactataaagactct" meltingTemperature="50"><Component hybridizedRange="2269-2288" bases="cgacaaactataaagactct"/></BindingSite></Primer><Primer recentID="5" name="KROL314  pdpA_qPCR_F3" sequence="acccaatatatcgtgaaaggct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite><BindingSite simplified="1" location="2067-2088" boundStrand="0" annealedBases="acccaatatatcgtgaaaggct" meltingTemperature="56"><Component hybridizedRange="2067-2088" bases="acccaatatatcgtgaaaggct"/></BindingSite></Primer><Primer recentID="6" name="KROL315  pdpA_qPCR_R3" sequence="gatagtgcgatacaaagatcaagtg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite><BindingSite simplified="1" location="2133-2157" boundStrand="1" annealedBases="gatagtgcgatacaaagatcaagtg" meltingTemperature="56"><Component hybridizedRange="2133-2157" bases="gatagtgcgatacaaagatcaagtg"/></BindingSite></Primer><Primer recentID="7" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="2773-2792" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="2773-2792" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="8" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="2853-2873" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="54"><Component hybridizedRange="2853-2873" bases="ttgcagttggcttagatacag"/></BindingSite></Primer></Primers>
  4<Notes>
<UUID>20eab8b0-684b-4583-b974-410b9c00303b</UUID>
<Type>Natural</Type>
<Created UTC="15:44:19">2020.11.11</Created>
<LastModified UTC="15:44:19">2020.11.11</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  7zXZ  ִF !   t/G] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!n	AQAi5\+.+Z83!Wz9|L&jغ:E䖻E"g>0R p2)&W>jt,t1BxATz
uրȦb6dtq	`mf,y,|zn?{ݽ_/eˏHjB;cRRhE
׈^"ڱdjW* QH(Ib^N[x̹v]PGGM/7ػe-;u&$Z;&S:'ȨBlJܦ++6t7TѶ6M
RoŸioy˫,*/ݻ7B)ck7:O!b_1z
O\؎58(52"*xY;gʉ($XVǯ(,PaX
yT_ VeCk*׶q8>rrr1O60iD{R|Ï##zڞ̉!ZvtEISlXƫ|saJ|,^%*0Tps~}nJ,Raqmz]o/ۇ܇_;rAXNsKtک6W~>ó%K1;"DñA" 5AJy}p"Yȼ:PޮCHko=mư/@ԟ"U}O{bP:!D:ʠhe8q^)E
,Ĵ"RvA3823Ovs6+]$AڙD&n4\mK,XcktE(S'bX2	1UpD^PQw{?N-f|tr;dRօ h?d?vYwf9ïKc"HlדmP%6^eI@q9y6#B\SZa	(._PM{lgp+t82P(%	64݊sy>/:Isa+A/f4G"6)>i3E4mfLϖzkB3;O/Xe`y2[6y c.|5RuMҙ.Rqi^~3/"!MǊ7UVN龥M&߸FeGJ;ؗZ;[}9'Xlv::p~P/$n䷁{j+$>CDOsp9eBK`Po޹H]%C1to,ajZ SnK<@hXp6&09)ʇg߮S
r|&"DB1=YWO:zac(mQS}Z6۴8|cM@-eJ$dmVDNQcvg*.aBJ[zX-vP+CӇr:zRyOæbsjO_ @6+ۂʑNG[OO9Y&R!3;A'^WH\CEuߦ&fgOGYaw
ɼl[h&>QŎx6k!qBX4uĞgM}<R'GfOS]1:l܀aOVxw?6)jWg+Yұ^SL^6rRꭵ'd!AXʺ7FtWJS!@x26S|0]%;	ښA`$	KyS-Nஐ80F!*rW|,P1~f2kxeD<ޏ%Hc$y%vM
0pt;(B!dc?*Dܙ=&K"H7"Y t-]mЅij[6z#.X$\/Ɖ"YJi1׻`"2CDW𬋀A=$SIʷ)PR <iy?PD|4GvбNcKLD,dNG)H)ߧ!sMLK)qv ^~|[bxˍ93P   {縡^x~  
g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  {<?xml version="1.0"?><Features nextValidID="13"><Feature recentID="0" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2581" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="1790-2581" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="2582-2650" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="1" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1030-1618" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2651-2755" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="T7 terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-812" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Possible TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="128-128" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="P345 JCOL221B AROL56 RT-PCR primer for" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="315-335" color="#8080ff" type="standard"/><Q name="note"><V text="P345 JCOL221B AROL56 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="6" name="P344 JCOL220B AROL55 RT-PCR primer for" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-254" color="#8080ff" type="standard"/><Q name="note"><V text="P344 JCOL220B AROL55 RT-PCR primer for tul4"/></Q></Feature><Feature recentID="7" name="lipoprotein" directionality="1" translationMW="15771.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="164-613" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKIIKLSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIKATVYTAYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKEVIASDSINI*"/></Q></Feature><Feature recentID="8" name="FTL_0421" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="164-613" color="#00e600" type="standard"/></Feature><Feature recentID="9" name="TSS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="126-126" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="109-127" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="16-63" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="to be cut out" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="82-88" color="#a6acb3" type="standard"/></Feature></Features>
  _<?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P344 RT-tul4_F" sequence="gttaggtggctctgatgatg" description="0"><BindingSite location="234-253" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="234-253" bases="gttaggtggctctgatgatg"/></BindingSite><BindingSite simplified="1" location="234-253" boundStrand="0" annealedBases="gttaggtggctctgatgatg" meltingTemperature="54"><Component hybridizedRange="234-253" bases="gttaggtggctctgatgatg"/></BindingSite></Primer><Primer recentID="1" name="P345 RT-tul4_R" sequence="ttgcagttggcttagatacag" description="0"><BindingSite location="314-334" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="55"><Component hybridizedRange="314-334" bases="ttgcagttggcttagatacag"/></BindingSite><BindingSite simplified="1" location="314-334" boundStrand="1" annealedBases="ttgcagttggcttagatacag" meltingTemperature="55"><Component hybridizedRange="314-334" bases="ttgcagttggcttagatacag"/></BindingSite></Primer><Primer recentID="2" name="KROL358  T7_amp_F" sequence="CTAGTGGTGCTAGCCAAAAAACCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:52:01Z"><BindingSite location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite><BindingSite simplified="1" location="1-24" boundStrand="0" annealedBases="CTAGTGGTGCTAGCCAAAAAACCC" meltingTemperature="60"><Component hybridizedRange="1-24" bases="CTAGTGGTGCTAGCCAAAAAACCC"/></BindingSite></Primer><Primer recentID="3" name="KROL359  T7_amp_R" sequence="GATCGATCCGCTAGCCTCGAGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T18:58:35Z"><BindingSite location="674-704" boundStrand="1" annealedBases="GATCGATCCGCTAGCCTCGAGC" meltingTemperature="53"><Component hybridizedRange="698-704" bases="GATCGAT"/><Component hybridizedRange="674-688" bases="CCGCTAGCCTCGAGC"/></BindingSite><BindingSite simplified="1" location="674-688" boundStrand="1" annealedBases="CCGCTAGCCTCGAGC" meltingTemperature="53"><Component bases="GATCGAT"/><Component hybridizedRange="674-688" bases="CCGCTAGCCTCGAGC"/></BindingSite></Primer><Primer recentID="4" name="KROL363  T7_seq_1" sequence="GTTATTGTCTCATGAGCGGATACATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:32:18Z"><BindingSite location="2688-2713" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="2688-2713" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite><BindingSite simplified="1" location="2688-2713" boundStrand="0" annealedBases="GTTATTGTCTCATGAGCGGATACATA" meltingTemperature="56"><Component hybridizedRange="2688-2713" bases="GTTATTGTCTCATGAGCGGATACATA"/></BindingSite></Primer><Primer recentID="5" name="KROL364  T7_seq_2" sequence="aacttgggcaactgtaacagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-12T19:33:45Z"><BindingSite location="501-526" boundStrand="0" annealedBases="aacttgggcaactgtaacagttaagc" meltingTemperature="60"><Component hybridizedRange="501-526" bases="aacttgggcaactgtaacagttaagc"/></BindingSite><BindingSite simplified="1" location="501-526" boundStrand="0" annealedBases="aacttgggcaactgtaacagttaagc" meltingTemperature="60"><Component hybridizedRange="501-526" bases="aacttgggcaactgtaacagttaagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>29ed4c85-fedb-49ab-8534-eab1650477d4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:16:25">2020.11.12</Created>
<LastModified UTC="19:33:45">2020.11.12</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO                E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
