	   SnapGene      AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGTACcttgtatggattagtcgagctaaaaagctcatattttttatattcaaactatatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgaacattcgtccattacaagatagagtattagttcgtcgtgcaggtacCatatgaattcaggaggactataatgctaagtattagagtagatgagcacaaaccatttgatataagtttaagaaacttcaaacgtgcttgcgaaaaagctggtatcaagcaagagcttagagataggcagcactacgtaaagccaacagaaaaaagaaagatagcaaaacgccaagctgttaaaagagcaagaatttctcagagaagagcatttatttaaGGATCCACTAGCTCGTTTCAAATTACCGATGATATCGGACCGTTCCAACTTACCGACCAGTTCGGCAGGTATGTATTTGCGTGCATTCCTATCCAAAAAAACATCAAGCCAAAAGCTTGAAAAAACTTACAACACAGCTCAACAGAGCTAGATTGTAAAACCCTGCTTTGTTAAGCAGAACGCAAAAATTGAATGACTTATAGTCATATCGCTTCGACCCTCGTAGATTAGTAGCCTTGAGCTATTAACTGGTTGAAACACTTACCAAATAAAGATTAAAAGCGATAAAAATGAAAGATAAAGCAGCTAAAAACAGAGATTTTAGAAAGACTATTTTATCAGTGTTACAACGCAATAAAGATGGCTCTTTTGCTACGCAAGCAAATAGAAAGTCTATTCTGTTGCAGGCAACTAAAGACCTTAAAAAGGTAGGGTTTAGCAAGGTTACAGCCGAAAACTTCGGTAATAAGCATTGCTATGCACTTAGAGACCATTGGAGAGCCCAAGGATTAGCTACAGCAACGATAAAAAATCGTTTAGCTTGTCTAAGGTGGTTAGGCGAGAAAATGGGCAAAGAACTACCCGATAATCGAAAATTAGAGATTGAGAACAGGAAGTATAGCGATAATTCAATCAATAAAGCCCAAGAAATCGATTTTAAGGCGATTTCTGCCTTAACTGATAGGCAAGCCCTAGCAATACAATTACAGCGCGAATTTGGGCTTCGTAGAGAAGAAAGTTTGAAGTTTCAGCCTAGTTATGCAATCAAAGAGCATAAAATCGAGCTTAAAAGCTCTTGGACAAAGGGTGGAAGACCACGAGAAATCCCAATTTTGAATGAAAAACAGAGAGAATTGTTAGAAAAAGTAAAAGAGGTAGCAGGTAAAGGCTCTCTAATTGAGAGCGAAAAGTCTTATAAGCAAGCAATGGAACATTTCACGACTCGCTGTCAAAGAGCAGGGATTAAGAATGTTCATGGCTTTAGACATGCGTATGCTCAAGATAGATATAGGCAATTAACAGGGCGTGAGTGTCCTAAAAATGGTGGATTAACATCTAAGCAGCTAACACCTGAGCAAAAGCAACAAGACTATGAAGCTAGAATGACTATTAGTGAAGAGTTAGGTCATGGTAGAGAAGATGTAACAGTCAACTACTTAGGCAGATAAAAAGCAATATAGCTATAGAAGAAAAGAAAGCTATTTTACATAGTAGATCGACTCTTCTTAGGGATTTTATATTTTTTGATAAATCATCTATTTTGCTAGTTAAATCATCAAATTTATCATCTTGTTGTTTGACTAAATCTAAGAATCTATTCTCTTTTTTAAAATCGTTCATGCAAACCGCCTATAGCTTTCTTCTTTTTCTGAAATTATTTGTCTTCACACCATAATTAAATTCCCATTTTTATAAGTAAAGTCTTTTAAAAGCTTGTCAGTCTCTTCTCTAGAAATGTACCAAATTTTACCTATTTTAGGATACTTTTCATGAAGCTCTTCTATTTTTCCCCAGTCCTTTAATAGTCTACCTTTAGAGTCTCGTAAATAGTTATCTTTGTGACAGGGGCCTCTTTTATCTTTTTTAATGTAACTATATGTTATTCCAACGTCACTATTACTATTATCCAAATCTTTTTTAGCATGCCAGTAAGAACTTTCATAACTTAACTCTATCTTTCGACCTCTTTGATATACAACAATAAAGCTATAGCCAGTAGTAACAACCTGTTTTACTTTTGTTAAATCTATTAACTTCTTATTTATTTTTTTATGTTTTTTTGAAAATTTAAATATTTCTATATTCATTCCTACACTTCCTCAAATCCAAATGGTAGCTTATGATTCTCTTCTGGTTTCTTTTCTAATTTTTTTATATTTGCAATAAAAACTCTTTTTCTATCTTTGATTTTTTTATTGTCCCAATTCCTCCAAGTATCATCACAAACCCTTTCAATATCATGTAAATGATGATGTCTAAATATTGATCTGACATAATACAGATCTAGGTCTAGTTCATCACTTAACACAACTTCTCTAAGTCTTTCAGATGCTTCGATTGGTATGTAATCCTCTTTATTTTTAGTATCTAAAAGCTTTTGCTTAAATTCTTCTTCTGTCTCTGCTACCTTACTAACTGTAAACTTGATATTTGTAATCTTACGACCATGTTTTCTGTGATGATCCTTGTCATCATAGGTTACAAAAATATCCGATAATTGATTAATCTCTTCTAGTGCTGGTAATAGGAACTTATTTTTAAAATTTGAATATCTGTTGCTGTAACTTTTAGGTAAATCAAAATCATTAATCATATCATCGACATACAATACGCAATCAACTATATTAGCATACCCTGCTTGTTCGCCTAATTTGCTTTTGAGAAGTAAGTATAATCTGCTTGAATACTTACTTTTAAATGAAAATAGTAACTGTCTTTCTGCTTTAGTAAAGTACTCTTGTAGTTGTATCATGTGTGGCATTAATGACCAATGAAACTCGCAAATTAAAGCACTGCTTTTAGGGTCTGCTTCAATATATGCAAACCAGTTAGCTATCTTCGTTTGTTCTTTATTCAGCCATACTGGCTTAGACATTATTGAGTGCATTAATTGCTTCAATCTCACTCTGTTATGCTTAACCCCTGTAGCTTTTTCAAGATCAGATAGGCTTATCTTATACCTGTGAAACTCTTTATCTTCTCTTTTAACCATTGAGGCAACTAAGAATATTAAGTTTTGTTCTTCTTTTGTAAGGCTATACTTTCCTGCAACAAGAGTATTAGACATAGCTATTTCTTTGCCAGCATTTACATTTTTAACTTCTTTCATAGAACTAGAGTCATTATCTCGATATACAAATTCTATAAAACTTCTATTAGTAAAACAACTACTTCATAAAAAAAAGTAGTTTTAACGATACAAAAAGTAGTTTTAAATTCAAAAAGTGATACAAAAAGTAGTTTTAAATTCAAAAAGTGATACAAAAAGTAGTTTTAAATTTTTTAAAAAAGTGCTTCAAAGCCTTATGTAGCAATACTTACAGAGGATTAAAAAAAAATCTGACAATATATAAAGAGAATATATAAAGAGAATATCTTAGGGGATTTTAAAAAAATCCCACAGACTCAAAGACTTTTTTGACTTTTTAAATCCTAGAAACTATACTTTAAGTACTTATTTAAGTACATGGATTTAGATTATGCAAACCGTTAATTATTCAACTTTTAGAAATGAACTATCTGATTCAATGGATAGAGTAACAAAAAATCATAGTCCTATGATTGTAACTAGAGGTTCAAAAAAAGAAGCAGTTGTTATGATGTCGTTAGAGGATTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCG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7!(B}^Nq] b ,k	kE% =aLU*   $[o?N_		g
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 c  >l@FMV ?   \'p>	
T66Q*O|(  &w~D`			
9Khax#X' @
5Di^z   %O8
|	Mi K {:%y|:~8cBTop #X*meev8\	q	w	wG5k{1@.  AS'				:
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Tsl> ['PeFht  M   AL93{/eiZF  K6K
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\(h63E B+

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O	o~-;l`y"J-  _&Wn	XiXm}&	w9Ze.s~ 	9I.pR+h<I  O
>m8& ,infy~o"	A17v#"`'s   \4{	0		
Fa~z t X,"=m		N
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nJf'D%w!    |Az.19		
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D\ZS] P# + | )]	0


#*/.<q#4g x  o	
b<-4@! |^

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 z-	4
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9s30 h  F ]	
P.MgP T `
6?  GCOFoL$= +Qm=2d  bGB";47JB#SY uS1Vrvp ro>e~="g_m ' >>.k+Eu<" = I Y	3dZ8c <s%4o
 H  *	|   1L9Z$ @d' 9m	Q2   mW  _ d ?Em	,- A'TG04    g`;DU1MN @   @Qe ?	 GZ{5 ]q
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6u!J aVE/DL9?k

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;keXY(H^  
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s04i	-\ny  E		>

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QT?S +
Uu/hWlr
   		tN  +r  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           	                                                                                                                                                                                                                         
                                                                                                                            	         "               	                                                                                                               4                     |   s         0         D   f                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                       	                                                                                   
                                            	   "     	                                     4       | s   0   D f   z  {  {  {  ~  s  a .3S\nCo +5k; PP W  Pa5P<=39O//9/
k
e.TBNd
R{GOA	
^,	*O?d B(  BSKn: %# #>L"
\Mep{y\tSKswkIkI- h %R[okI$cTnSKN	%
	%j	w}z  SK
  B$A	dpSKn:>e
z vAk".E@SND=2scowkIZ
{6 S r	E9Ju
@Ot	$
jW 
a}R
Fm{Mm;By\twLG 1P	~ukaIF tP	B]-YPOh	E@4 L:/}4 L#

a=Fh(	mKJg$M%F i&	i
3 RH$9f.HB	P}OQ	K<-HP}3 RH.$9f }Hg23 RH$/369%"f.X OQ	in>$,+X,K :%y8co\			0|GfS2q
J{:QTcaEDS??<2qX wb}A S ( C$	V
9W(19F|
!s)Xe,0-h{;	Y	n		w3QlN{^s[
`c +$	9W;	n	3( CV
(1/36%"XX wb}AJFihx* \	/
-p3E  mW  f0&S3S  *36N"n<1<X C})5 u   2Yy-T#>3;Jln	O
Q{VWMLUV  ^ TDL+~,[+n

6u!J%

Fa-N$ C	C$?Zlw FB:PlIARK	# ^	
$Mq$ 5 u     2Yy-T#>3;JlnC			O
Q4={HVdCWMj #<'8K$?ChLUr8QV@Zlw     %'KMOGIZi )g^k^	`					
+
S
u


2+6IQz7k/HSr!,.}-1$=_M^kqhjlDl	?{&2 >u`{
O	o~-;l`y"Jg   EfM8vax#
m

]eUNfq|F[Z E		>

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S喛fj3NVB9|g3oˉ 92۶	s.(6q&Đf>G[tBX,eyG奀Nu6svh+a`ͽg4x=Z GXFKvi&$s?.u̽\v@6q    $ <  p]g    YZ       D7zXZ  ִF !   t/] ˇf{3(1v%f M*	mj)p80*jBJA>f"AvӒp&s{3Th$h|=27|r6
ͽ;29SNx*mq:Mҁ-76)_g+TST~6n]JHbG>8O< #dr]GA/lYkLS4gp_1\9e`mP%	".J{iZ~   `   Gg    YZ   /       &   -         -0fxfadi	Y         /    mseylab/Library/Caches/SnapGene/3100_97.tm  7zXZ  ִF !   t/!] 3D].?I*ә_.ZvќP,Vj,ު~mC<ґ?vd;!U''yzYl.vU[Qec <L3cGq~=X?r+<ɵؿĊG]1|=ty?izd@Pp7z0ݽ<,c^D2)=0(w+}[)0|cK7tDi|6?i6J$MEԎ.j7ȡdw}fHAKnm/<`!H|r,7\LD'jY̖ǯYx/v\y0xu Bi8|2f=Uf'C(0s!A	Q&-Ԅ9y{. Y;H#B M`C>H/	¾ ?d:2Ĭc,엋 { q7<7Tǯiv`8./ZDYApM^M/n*GH*\*Oa4]FO-ؗYGBU؜¼@$LD[-,6𼻝p
7ɚm?TaPOǯj> 	gy3˺ai|ti胪pv/T% 0`5MIfY6s5~iUU<y6rm$fg|)ADj$G-xL{q.nӖ6v"V$2V^e}b%բmϒ+.܋]*7 ]_ކp:?d$nߣOv}<֥fe!W Ɏ'8ZI~8OP E.9oFAW:-v';!d.C?Z7/<ʜIXܦK3jɬ.@EڸQsЃM75_\Y&Fv@٢֤	HPi&
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߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
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UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
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߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  87zXZ  ִF !   t/6m] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!n략W7ܿ׵C/jpk e  9.6b:6l#% Xzh){=\kϣ*va52Ա[l."aᰨd:,N储H=zM) 9)OLpFo@o·R΃[3}㍊oFtz5"Uf9mjT0yGxT;ܛ9~>Deٷ\	@vɄ2UhMIӺ]eVSֻKUO VRisr}TCjjwlA˵IYXWTw|2mJ8LѫQ_؟RV2usUb=['oI`6F6	ʩf@3hW^JC 	@ukd&Gʽ E q9|R_x!ۑM>xfu݃+q4l夝h#w_ܲDػ9Vk%m@L{H=eb
*31d
 sөXݠ ova.Cg)}»ɍlM{x52t~;	05jq,$q$Y2OqilMo6DH}JԺ[7oo)~JT̚/N0L,L^C
+%IzfofLBםSOc9DrNIǇW z7rrۼT5E2#.J;}:lM3̳x`o8$ 1:y0[h47@yiHKCsbh2K0\N4<Txbu떒_+]O	=2}4Quh1"e&ǌ;9kQًjNQÆisZ(y9y4^&MdxdSTeL	Vb[wW~{rJ~G4PK<5wJA$uQhlVt3DxtE2ԑ> 	ӭŧA&N+buŨ @v[V-Q&U\^[k.ɾFcJK\%#*GWh#y}"#D7F$[]i_*ΒQ?E1u/owD&/gQϖfYzú7z~Fɝ$=2_2;7ޭQQBG)Rd=6zhsGj<GhMqORR6]@s-@[K/(09P5b-`t>?3jZnu{ UekȽp^'ice}$ԧ3ggԭd<&FPcic*=ͦ|q Ҙ=rg      U, l  ug    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="17"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V/></Q></Feature><Feature recentID="3" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="804" maxFusedRunOn="804"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MLSIRVDEHKPFDISLRNFKRACEKAGIKQELRDRQHYVKPTEKRKIAKRQAVKRARISQRRAFI"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="L*"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1095-1970" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2193-2612" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2618-3634" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4813-5604" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5625-6482" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-952" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4675-4724" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7425" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL44 Seq_pF_1R" sequence="TAGCTCTGTTGAGCTGTGTTGTAAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="929-953" boundStrand="1" annealedBases="TAGCTCTGTTGAGCTGTGTTGTAAG" meltingTemperature="59"><Component hybridizedRange="929-953" bases="TAGCTCTGTTGAGCTGTGTTGTAAG"/></BindingSite><BindingSite simplified="1" location="929-953" boundStrand="1" annealedBases="TAGCTCTGTTGAGCTGTGTTGTAAG" meltingTemperature="59"><Component hybridizedRange="929-953" bases="TAGCTCTGTTGAGCTGTGTTGTAAG"/></BindingSite></Primer><Primer recentID="1" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-800" boundStrand="1" annealedBases="aataaatgctcttctctgagaaattctt" meltingTemperature="56"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="773-800" bases="aataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-800" boundStrand="1" annealedBases="aataaatgctcttctctgagaaattctt" meltingTemperature="56"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="773-800" bases="aataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="2" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer><Primer recentID="3" name="KROL354  pF_rpsU1_R" sequence="atatGGATCCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-11-05T15:58:35Z"><BindingSite location="775-810" boundStrand="1" annealedBases="tGGATCCttaaataaatgctcttctctgagaaattc" meltingTemperature="60"><Component bases="ata"/><Component hybridizedRange="775-810" bases="tGGATCCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="775-810" boundStrand="1" annealedBases="tGGATCCttaaataaatgctcttctctgagaaattc" meltingTemperature="60"><Component bases="ata"/><Component hybridizedRange="775-810" bases="tGGATCCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="16:23:57">2020.11.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                                     HO  d             E             Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
