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ATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG  DS                     %               	         %         #      (   %   7      !   "   '   ,    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	
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Yja< 
,2e%bY<|) ~)L8ax/6AD [R<	;

%]#Hq J\d
}lV	k	s	`
f
?s3  $<&				3
n


(QoD1y<Fm)1}8Y>j L	B]th']~(   {t6gfT

.1j2G%G@* B{O-\	
(x0f(c9:ik,L   4|XO	u



8F^ovEdz8S7R\qK}PB./  -$



%%b:O ,HOSXUu"  V&Oj`@'W w { Idn/	f5b=Sf:"  d
s


#m=bOo:	  j"5  lmiawphyr0J & H v$db;
6 ErwZ0   $U7
WMz/Z  2<<rgW`{
w&   `'Q*=BwSA
m%   !F5

]G|# Ozyd	-3W6 Fy		}$7w.X;( ;U	
	\;li%  ,{#N57EW[09AT-  i%leae9  G vv	x   <@
1x,{"fz +$|V:~8@
   0u  .  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                     	                                                                                                                                                                                                                                            	                                                                                                   
         &         	      	                                                	                                                                     7                         x         B   X   5         J   n                                                                                                                                                                                                    
                                                                                                                                                                                                                                                                           
                                                   	                                                                                        	                                   
   &   	  	                	                       7         x   B X 5   J n   p  b  v    D  X  4> U		:Zz- j
#o R\b	 ww	 W  D0w\wc8dZ`(:vtV)
-V)
`=-V)
-!%	$+1.{i udEFy	m"$nvh
S|*@Qv?& BO  Br zra L# !JeL:2I

C tr zrF pp- h Lyo9pK ={r zru
CL6 1L	
<  Fr zr  Br Khzrae6%@/	:1 l7A#
h
IUA!lg T!zuk=!)YF# o=9pA#

#	$6 S ;l-`q -1gFv7#$	K)(W 170y

Fmtb iKAL2GA	 1
w|*A	@pF tP0	
iT$w;v4lg4 7saV:4 s6/	%:@J	
A(Orq
KtLm*.6[G/?$-*& 
G
M13 Myo	K`CU
oiwlvx0rcTow3 Myo	U
K`CG0o
DY3  #Myo	KVZ]`LI.CU
 lvx0
ejKSR*7SK Ba)L6_ 2<WnBf')In=Gz	Yq	ax{InG-lk	z		
f
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  <?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7975" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5225-5274" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1480-1502" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="4" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6175-7032" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="5" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5363-6154" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="6" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3168-4184" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="7" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2743-3162" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1645-2520" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="10" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="836" maxFusedRunOn="836"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="TEV site" directionality="1" translationMW="869.93" type="CDS" allowSegmentOverlaps="0" cleavageArrows="931" consecutiveTranslationNumbering="1" maxRunOn="1354" maxFusedRunOn="1354" detectionMode="exactProteinMatch"><Segment range="914-934" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;tobacco etch virus (TEV) protease recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="CBP" directionality="1" translationMW="2964.51" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1354" maxFusedRunOn="1354" detectionMode="exactProteinMatch"><Segment range="809-886" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;derived from skeletal muscle myosin light chain kinase; binds calmodulin with nanomolar affinity in the presence of calcium&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;calmodulin-binding peptide&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="ProtA" directionality="1" translationMW="6483.18" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="1354" maxFusedRunOn="1354"><Segment range="1151-1321" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;IgG-binding unit of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; protein A&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="ProtA" directionality="1" translationMW="6640.34" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1354" maxFusedRunOn="1354"><Segment range="974-1147" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;IgG-binding unit of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; protein A&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="rpoC-TAP" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="809-1351" color="#993366" type="standard"/><Q name="label"><V text="rpoC-TAP"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-808" boundStrand="1" annealedBases="tGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="66"><Component bases="ata"/><Component hybridizedRange="771-808" bases="tGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-808" boundStrand="1" annealedBases="tGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="66"><Component bases="ata"/><Component hybridizedRange="771-808" bases="tGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer><Primer recentID="2" name="KROL218 rpoC-TAP-ckR" sequence="GTGTTGCGCAAGAGCCGCGGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T18:57:40Z"><BindingSite location="942-963" boundStrand="1" annealedBases="GTGTTGCGCAAGAGCCGCGGTT" meltingTemperature="68"><Component hybridizedRange="942-963" bases="GTGTTGCGCAAGAGCCGCGGTT"/></BindingSite><BindingSite simplified="1" location="942-963" boundStrand="1" annealedBases="GTGTTGCGCAAGAGCCGCGGTT" meltingTemperature="68"><Component hybridizedRange="942-963" bases="GTGTTGCGCAAGAGCCGCGGTT"/></BindingSite></Primer><Primer recentID="3" name="KROL333  TAP_tag_forward_notI" sequence="atatgcGGCCGCAAGAGAAGATGGAAAAAGAATTTCATAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-08T17:44:52Z"><BindingSite location="800-835" boundStrand="0" annealedBases="gcGGCCGCAAGAGAAGATGGAAAAAGAATTTCATAG" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="800-835" bases="gcGGCCGCAAGAGAAGATGGAAAAAGAATTTCATAG"/></BindingSite><BindingSite simplified="1" location="800-835" boundStrand="0" annealedBases="gcGGCCGCAAGAGAAGATGGAAAAAGAATTTCATAG" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="800-835" bases="gcGGCCGCAAGAGAAGATGGAAAAAGAATTTCATAG"/></BindingSite></Primer><Primer recentID="4" name="KROL334  TAP_tag_reverse_bamHI" sequence="ATATGGATCCTCAGGTTGACTTCCCCGCGGAAT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-08T18:03:47Z"><BindingSite location="1331-1360" boundStrand="1" annealedBases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT" meltingTemperature="70"><Component bases="ATA"/><Component hybridizedRange="1331-1360" bases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT"/></BindingSite><BindingSite simplified="1" location="1331-1360" boundStrand="1" annealedBases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT" meltingTemperature="70"><Component bases="ATA"/><Component hybridizedRange="1331-1360" bases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="18:50:52">2020.9.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  M<?xml version="1.0"?><Features recycledIDs="2" nextValidID="14"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7429" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4679-4728" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-956" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="4" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="5" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5629-6486" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="6" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4817-5608" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="7" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2622-3638" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="8" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2197-2616" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="9" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1099-1974" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="959" maxFusedRunOn="959"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-807" boundStrand="1" annealedBases="GCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="771-807" bases="GCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-807" boundStrand="1" annealedBases="GCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="771-807" bases="GCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="18:8:28">2020.9.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
  -     u  *        *Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf@2iQQ,FQ1T8I^rgU=zsUUxUQ5dP0fxfadi	YAVfz|}+	U]=TU^wNDhsUhXzdl쑤Nix(Wz}YQSUFTӕ]EG)5;9MY.k45GU	)^Sh7GONtUl:.PU[iAIk[YSPƮ>CS5i1jTR>Jc[TMlNNP Q[]ETD$ЕZ1Te55vǳTM+`Gc&;QitV]M9w5S]GSaJB$EK^zCFUMfNdQTT$l{jem9)[mZn*V滪[+cWϙ:wiZIRNKEb}Zz;jD%d!b^f)\yncQz^fuTOI%V旮UY[ՂNk૪媩eWjhZԛmfؕYvYѹޗuKF:Ƃb[ITꎕ9yxf[bamUf񖆖#Y骕jn(^	[UٶgYmyM㢳RReYꕅY%%I(b X}a^ZT:_JNnnNm~!^n_hC^Qf+9uiMکz-fukWi%]yeURJuTZRRJkUHgUI*U#S5iUUufeDY@UoEy''`jFתEaykX]UmyUQM(rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]V9PSܑSEpabӞ\Q4$`NG©Sh(?RWPn3@QmDtAh]F
:CØxG[;<+ËL+Q#X^4CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H    (  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="14"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7466" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4716-4765" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="810-842" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="971-993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="4" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="5" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5666-6523" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="6" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4854-5645" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="7" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2659-3675" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="8" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2653" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="9" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7840.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="845" maxFusedRunOn="845"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="18:7:51">2020.9.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
       u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:52:23">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
ܒ?G
?r/rHWuꆦPƬ>h)Ua8j]UsuZ3P8ALrEOK8NxnPsS5DTГH9
ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
ܒ?G
?r/rHWuꆦPƬ>h)Ua8j]UsuZ3P8ALrEOK8NxnPsS5DTГH9
ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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- 'MU67kΡPh=_x/[͗JX*qѧ 1|}ndd^M$!v{u!=(N 1<KA0x1X:iz__|;\Ĉ+;KdΖRoCCr[ԀR\Em*     5MG&l  )njıg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  F<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="TAP" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="810-1352" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="rpoC-TAP"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="ProtA" directionality="1" translationMW="6640.34" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1355" maxFusedRunOn="1355"><Segment range="975-1148" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;IgG-binding unit of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; protein A&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPK"/></Q></Feature><Feature recentID="2" name="ProtA" directionality="1" translationMW="6483.18" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="1355" maxFusedRunOn="1355"><Segment range="1152-1322" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;IgG-binding unit of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; protein A&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNGAQAPK"/></Q></Feature><Feature recentID="3" name="CBP" directionality="1" translationMW="2964.51" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1355" maxFusedRunOn="1355" detectionMode="exactProteinMatch"><Segment range="810-887" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;derived from skeletal muscle myosin light chain kinase; binds calmodulin with nanomolar affinity in the presence of calcium&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;calmodulin-binding peptide&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KRRWKKNFIAVSAANRFKKISSSGAL"/></Q></Feature><Feature recentID="4" name="TEV site" directionality="1" translationMW="869.93" type="CDS" allowSegmentOverlaps="0" cleavageArrows="932" consecutiveTranslationNumbering="1" maxRunOn="1355" maxFusedRunOn="1355" detectionMode="exactProteinMatch"><Segment range="915-935" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;tobacco etch virus (TEV) protease recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="ENLYFQG"/></Q></Feature><Feature recentID="5" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1355" maxFusedRunOn="1355"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKR"/></Q></Feature><Feature recentID="6" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="9" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1646-2521" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="10" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2744-3163" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="11" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3169-4185" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="12" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5364-6155" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6176-7033" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="14" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="15" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1503" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5226-5275" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7976" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="18" name="bS21-2-CBP" directionality="1" translationMW="12758.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1355" maxFusedRunOn="1355"><Segment range="606-932" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKRAAAKRRWKKNFIAVSAANRFKKISSSGALDYDIPTTASENLYFQ"/></Q></Feature><Feature recentID="19" name="bS21-2-TAP" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="606-1355" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKRAAAKRRWKKNFIAVSAANRFKKISSSGALDYDIPTTASENLYFQGELKTAALAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNGAQAPKVDANSAGKST*"/></Q></Feature></Features>
  ,<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-807" boundStrand="1" annealedBases="GCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="771-807" bases="GCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-807" boundStrand="1" annealedBases="GCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="771-807" bases="GCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer><Primer recentID="2" name="KROL218 rpoC-TAP-ckR" sequence="GTGTTGCGCAAGAGCCGCGGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="943-964" boundStrand="1" annealedBases="GTGTTGCGCAAGAGCCGCGGTT" meltingTemperature="68"><Component hybridizedRange="943-964" bases="GTGTTGCGCAAGAGCCGCGGTT"/></BindingSite><BindingSite simplified="1" location="943-964" boundStrand="1" annealedBases="GTGTTGCGCAAGAGCCGCGGTT" meltingTemperature="68"><Component hybridizedRange="943-964" bases="GTGTTGCGCAAGAGCCGCGGTT"/></BindingSite></Primer><Primer recentID="3" name="KROL333  TAP_tag_forward_notI" sequence="atatgcGGCCGCCAAGAGAAGATGGAAAAAGAATTTCATAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="800-836" boundStrand="0" annealedBases="gcGGCCGCCAAGAGAAGATGGAAAAAGAATTTCATAG" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="800-836" bases="gcGGCCGCCAAGAGAAGATGGAAAAAGAATTTCATAG"/></BindingSite><BindingSite simplified="1" location="800-836" boundStrand="0" annealedBases="gcGGCCGCCAAGAGAAGATGGAAAAAGAATTTCATAG" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="800-836" bases="gcGGCCGCCAAGAGAAGATGGAAAAAGAATTTCATAG"/></BindingSite></Primer><Primer recentID="4" name="KROL334  TAP_tag_reverse_bamHI" sequence="ATATGGATCCTCAGGTTGACTTCCCCGCGGAAT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1332-1361" boundStrand="1" annealedBases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT" meltingTemperature="70"><Component bases="ATA"/><Component hybridizedRange="1332-1361" bases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT"/></BindingSite><BindingSite simplified="1" location="1332-1361" boundStrand="1" annealedBases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT" meltingTemperature="70"><Component bases="ATA"/><Component hybridizedRange="1332-1361" bases="TGGATCCTCAGGTTGACTTCCCCGCGGAAT"/></BindingSite></Primer><Primer recentID="5" name="KROL335 pF_seq_NEW1" sequence="ttgactcaatatctagacttgcaagag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="423-449" boundStrand="0" annealedBases="ttgactcaatatctagacttgcaagag" meltingTemperature="57"><Component hybridizedRange="423-449" bases="ttgactcaatatctagacttgcaagag"/></BindingSite><BindingSite simplified="1" location="423-449" boundStrand="0" annealedBases="ttgactcaatatctagacttgcaagag" meltingTemperature="57"><Component hybridizedRange="423-449" bases="ttgactcaatatctagacttgcaagag"/></BindingSite></Primer><Primer recentID="6" name="KROL336 pF_seq_NEW2" sequence="ttaaagttaaaatcgagagcttgtttga" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="360-387" boundStrand="0" annealedBases="ttaaagttaaaatcgagagcttgtttga" meltingTemperature="54"><Component hybridizedRange="360-387" bases="ttaaagttaaaatcgagagcttgtttga"/></BindingSite><BindingSite simplified="1" location="360-387" boundStrand="0" annealedBases="ttaaagttaaaatcgagagcttgtttga" meltingTemperature="54"><Component hybridizedRange="360-387" bases="ttaaagttaaaatcgagagcttgtttga"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="20:21:35">2023.1.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unique 6+ Cutters                                                                                           d     CHF  d              E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
