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GTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAAGgatccactagtgagctcatgcatgatatcgtccgggccgcaagctcctagcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctcaagctagagagtcattaccccaggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccctgccactcatcgcagtctagcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagctcagctaattaag  E                  &   +   
   #            3   &                  .   %   2      "   ;   (   /   %                                     
   #      ,                  /   /   "   2   +       #      G            )   '   )                                    )      3                           N   8   ,   &   -          '   (   )      5   .            .   !         .   7                $             +   !            (            1   !            ,   *   ;   "   /   A                       $   &   !   
               (   $   #         -   "                  .      '      ,   -   %   -   -   X   0             "            %   (             +                                  (      *   .                  ,   !   3   8   !   "   -   +             %                        %   A   7      +                     (                     *      6   '            (      "      $                 ]

QL Df^

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M0Vy"'pYyIpx*&*hS[s 	
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]

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	

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O5`@3 -g	
V	6WhaV8v$#lME*d :  1n6 		

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v*@,Mm $~
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(	.		
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2Rj/=b		


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:%.^o ,1XeT( * T   gkB^d	T


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	,Y5GHIu2niKl?  9mG


"xg\'kI5 jz M3~#P>"


AZ.9HF7Dp. 4F'7V5			'
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) D [t(8@&_.  % R 	W,;IY#8;E
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8t1J*u`Qz
  -;	
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pA9AW3$ 9`/p		|oG(7O%T.zS7  B
<0HT0]*~ *%
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&BR50
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sJDO'X{MTgS\c9%DUoq"  5Wu			L
K[bT6>	k$/  @8&	6	

wIt*\^RB IuA l rDG%*3M-k6	P

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j

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<lJZ,		LVi	!;l{v`jQ$ 5 1X-ai.9<6d	,a6]k.dN# S 
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71Q	
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k+rs% Q~		
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b f,Y`		i 
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ZS%  ViH	


Co26&1@A 7I
'"	[	=rvICNK&Wz(;CJyfRb+]iTn/7 k qC2,j5	i

Jt[Z+'HAtj# 1  <eB9	
.gz1pzqu+ \ [ee	@
X
_h#q*WMY\    {4F#roC  	`
 kc_VM = q=f r		_&5 0@S~~U1/ _     Ms		
1=R 0E
b
Ow4(  \QzW	
I96HScM k1cp $!	f
'\k[Mlqz QR	
d	_jr  Tg{0Z	a		
?Ycn2H "Xh!7( @-z dk	
+n$UB9*  d '?	{
?Eu qQzGjz5)B  ;;	x<a s~Mz!6 XJp	
.l9(3~(K3~.' t}		
-)S!8Gj~PK^ e	^

63`0~C 9+bC
I

+^xOU  X
Y	lF[?(  B;1	
	
Bgs%VtQ\G zq?R%U." /	r	
j~k5GR<Ly0o !/'m$ c		*t-ki4'}} J 	,/}*E>] pir$KxT bo	q,IJ||   zTTTN   {TTNT   |TNTA   }NTAG  TCAN  CANG  ANGC  NGCC  TGAN  GANT  ANTC  NTCA  &CGAN  'GANG  (ANGC  )NGCA  /6  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                         &                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
                                                                                                                                                                                                                                                           #                                        	                                           !                                                                                                                      	            6      
               	      	                  	                         
                                              ,   6   /   
      2      %            s   ]      $             ,   R                                                                                                                                                                    &                                                                                                                                                                                                                                                                                                            
                                                                                                 #              	               !                                          	    6  
     	  	      	         
                , 6 / 
  2  %    s ]  $     , R     >  J  p  v  "  Ox1?x5G,-'?~x2	V L;L	g	"i"
hD	))L
Lh(nYw
U5FWL   D

2_	>X	Z G'APGq
7	x> P GxIeu	Y
Uc	
[
  `J5	MI	e]	#\SD		"#SD	"#Qc44USD	"D#o1G	xs <J]e
S~1hv_#Mbk
>(s	p
rH(bc$F[\oj

,IK \	p
rH(0gSD		"D#3\"sd6|q(MRVjy&is'OS^UF[\b]	n	nz1CP:
XZ YV
c	TgF6Nd#8f Q5	(MP9<VWYJ IS1Mu6u5SD	:"#i6=   AH0#3)s:Cg3d&Pf	.T((QdQeTQuQs	 Cy
		A	]
~ F
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consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7656-8013" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7656-7684" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7707-7804" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7901-7995" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="61" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7650-7654" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4859-4882" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5651-5680" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6533-6557" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7177-7201" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="66" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4583-7654" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="67" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4583-7654" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="68" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4600-4616" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4583-7654" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4583-7654" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="75" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="76" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="77" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="78" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="81" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4582" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4491" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="72" name="P575 Pbfr_1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4552-4579" color="#50ff50" type="standard"/><Q name="note"><V text="P575 Pbfr_1R"/></Q></Feature></Features>
  ><?xml version="1.0"?><Primers nextValidID="25"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4616-4635" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4616-4635" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4616-4635" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4616-4635" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7628-7653" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7628-7653" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7628-7653" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7628-7653" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4819-4840" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4819-4840" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4819-4840" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4819-4840" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5341-5365" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5341-5365" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5341-5365" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5341-5365" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5867-5887" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5867-5887" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5867-5887" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5867-5887" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6389-6411" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6389-6411" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6389-6411" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6389-6411" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6913-6934" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6913-6934" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6913-6934" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6913-6934" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7443-7466" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7443-7466" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7443-7466" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7443-7466" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7629-7660" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7629-7660" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7629-7660" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7629-7660" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="20" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4582-4603" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4582-4603" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4582-4603" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4582-4603" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="17" name="P575 Pbfr_1R" sequence="ATATCATATGgttacctccattatttaaaactctaatc" description="Contains NdeI site"><BindingSite location="4551-4578" boundStrand="1" annealedBases="gttacctccattatttaaaactctaatc" meltingTemperature="53"><Component bases="ATATCATATG"/><Component hybridizedRange="4551-4578" bases="gttacctccattatttaaaactctaatc"/></BindingSite><BindingSite simplified="1" location="4551-4578" boundStrand="1" annealedBases="gttacctccattatttaaaactctaatc" meltingTemperature="53"><Component bases="ATATCATATG"/><Component hybridizedRange="4551-4578" bases="gttacctccattatttaaaactctaatc"/></BindingSite></Primer><Primer recentID="22" name="P296 Pbfr_R" sequence="atatGGTACCaaaatttaattctttataagttatcaatttag" description="0"><BindingSite location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatGGTACC"/><Component hybridizedRange="4459-4490" 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bases="tgttacctccattatttaaaactctaatc"/></BindingSite></Primer><Primer recentID="24" name="P546 Pbfr-R" sequence="atatCATATGttacctcctaaaatttaattctttataagttatcaatttag" description="&quot;Contains NdeI site, adds optimal RBS&quot;"><BindingSite location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatCATATGttacctcct"/><Component hybridizedRange="4459-4490" bases="aaaatttaattctttataagttatcaatttag"/></BindingSite><BindingSite simplified="1" location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatCATATGttacctcct"/><Component hybridizedRange="4459-4490" bases="aaaatttaattctttataagttatcaatttag"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="16:58:5">2020.2.4</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c          	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFyOa*rx_^ϣM=82Y1D?7Ҍ`Ń:STH/WF7
q\[*+[{;vTACZj){n p3Aʏ.LکE_>!8)mbL6r죍um1أΥ88Rb0ģ!]*`!\г>0i <lw\W^ L[n
<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"YA퍆l5;LFUC>jSʔUi.NYUs_+սBx]NӖ      n  p     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7537-7565" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7588-7685" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7782-7876" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="61" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7531-7535" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4740-4763" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5532-5561" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6414-6438" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7058-7082" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="66" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4464-7535" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="67" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4464-7535" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="68" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4481-4497" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4464-7535" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4464-7535" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="75" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="76" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="77" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="78" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="81" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  3<?xml version="1.0"?><Primers recycledIDs="17" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4497-4516" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4497-4516" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4497-4516" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4497-4516" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7509-7534" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7509-7534" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7509-7534" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7509-7534" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4700-4721" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4700-4721" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4700-4721" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4700-4721" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5222-5246" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5222-5246" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5222-5246" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5222-5246" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5748-5768" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5748-5768" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5748-5768" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5748-5768" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6270-6292" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6270-6292" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6270-6292" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6270-6292" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6794-6815" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6794-6815" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6794-6815" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6794-6815" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7324-7347" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7324-7347" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7324-7347" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7324-7347" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7510-7541" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7510-7541" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7510-7541" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7510-7541" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="20" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4462-4484" boundStrand="0" annealedBases="aGCCATGATTACGGATTCACTGG" meltingTemperature="60"><Component bases="gttctaattaagtagacaatgatagc"/><Component hybridizedRange="4462-4484" bases="aGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4462-4484" boundStrand="0" annealedBases="aGCCATGATTACGGATTCACTGG" meltingTemperature="60"><Component bases="gttctaattaagtagacaatgatagc"/><Component hybridizedRange="4462-4484" bases="aGCCATGATTACGGATTCACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="16:53:58">2020.2.4</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="77" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="78" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="81" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature><Feature recentID="60" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4486" color="#a6acb3" type="standard"/></Feature><Feature recentID="73" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7567" maxFusedRunOn="7567"><Segment range="4487-4495" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  3<?xml version="1.0"?><Primers recycledIDs="17" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4529-4548" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4529-4548" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4529-4548" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4529-4548" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7541-7566" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7541-7566" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7541-7566" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7541-7566" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4732-4753" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4732-4753" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4732-4753" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4732-4753" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5254-5278" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5254-5278" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5254-5278" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5254-5278" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5780-5800" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5780-5800" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5780-5800" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5780-5800" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6302-6324" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6302-6324" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6302-6324" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6302-6324" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6826-6847" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6826-6847" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6826-6847" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6826-6847" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7356-7379" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7356-7379" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7356-7379" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7356-7379" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7542-7573" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7542-7573" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7542-7573" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7542-7573" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="20" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4468-4516" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4468-4516" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4468-4516" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4468-4516" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="16:52:37">2020.2.4</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFyRZRGXdiʧ\W2ץvNLQt4X1`Σ}<881Cxэc?nw!iʊݩb89Ps`^*ۈ#\,)t˅~6s<Wϳ`3!JnA(e|y[Lj(a<'1(yJ4W4,|ϫ!Zla2)2.,UW<V[0w78hSE>1
#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S              -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ]<?xml version="1.0"?><Features nextValidID="83"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" 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name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7927" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7598" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning 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terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4464" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="61" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7568" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4773-4796" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5565-5594" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6447-6471" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="66" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7091-7115" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="67" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="68" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4497-7568" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="69" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4514-4530" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="72" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="73" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#00e600" type="standard"/></Feature><Feature recentID="74" name="hypothetical protein" directionality="1" translationMW="333.45" 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type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="81" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="82" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  7<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4530-4549" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4530-4549" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4530-4549" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4530-4549" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7542-7567" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7542-7567" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7542-7567" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7542-7567" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4733-4754" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4733-4754" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4733-4754" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4733-4754" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5255-5279" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5255-5279" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5255-5279" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5255-5279" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5781-5801" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5781-5801" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5781-5801" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5781-5801" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6303-6325" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6303-6325" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6303-6325" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6303-6325" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6827-6848" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6827-6848" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6827-6848" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6827-6848" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7357-7380" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7357-7380" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7357-7380" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7357-7380" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4472-4517" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="62"><Component hybridizedRange="4472-4495" bases="ctaattaagtagacaatgatagca"/><Component bases="ATG"/><Component hybridizedRange="4496-4517" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4472-4517" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="62"><Component hybridizedRange="4472-4495" bases="ctaattaagtagacaatgatagca"/><Component bases="ATG"/><Component hybridizedRange="4496-4517" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7543-7574" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7543-7574" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7543-7574" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7543-7574" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="18:1:53">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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-z<#K!*S)֣&!c0{VXXlrVpb1^A/9 y$͎|M3~=$Gڐ8&$֖-bI	l93=40&)=8@r +yz;oM2=1s|zm]:1^VwUHvdTvfζepz(#:r^T1I|!SR6ԞT9D\:?N1YY-Z8͔9gj)WH>GG2*rU0j|VbխޫFMzUx2_7~D?f2*eGtHkҩoɟMu`t}e.2*$#;#TXw3\EeѤG~׼~; Q5wnˍKn9	ϕ/XPL+U5KZAf#܎r%hUD*(KR䞸{uY}X`(9P\܈Å9uJ]^0Avt/CYhۻ܁%a>ҧ.q:·qV*Q!Du:}U4꓏}zJ}ʎlB乗LB7^=xYD    3 ժ pg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  dv<?xml version="1.0"?><Features nextValidID="82"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7665-8022" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7665-7693" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7716-7813" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7910-8004" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7659-7663" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="60" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4868-4891" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="61" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5660-5689" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-6566" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7186-7210" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="64" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-7663" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="65" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4592-7663" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature recentID="66" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4609-4625" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="67" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-7663" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="68" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-7663" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="69" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="70" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="71" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="72" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="73" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="74" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="75" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature><Feature recentID="76" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4582" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="77" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4491" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="P575 Pbfr_1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4552-4579" color="#50ff50" type="standard"/><Q name="note"><V text="P575 Pbfr_1R"/></Q></Feature><Feature recentID="79" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4583-4591" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="80" name="FTL_0617" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4583-4591" color="#00e600" type="standard"/></Feature><Feature recentID="81" name="hypothetical protein (Bfr)" directionality="1" translationMW="391.49" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7663" maxFusedRunOn="7663"><Segment range="4583-4591" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MEL"/></Q></Feature></Features>
  Tj<?xml version="1.0"?><Primers nextValidID="33"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4625-4644" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4625-4644" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4625-4644" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4625-4644" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7637-7662" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7637-7662" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7637-7662" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7637-7662" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4828-4849" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4828-4849" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4828-4849" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4828-4849" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5350-5374" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5350-5374" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5350-5374" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5350-5374" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5876-5896" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5876-5896" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5876-5896" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5876-5896" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6398-6420" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6398-6420" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6398-6420" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6398-6420" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6922-6943" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6922-6943" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6922-6943" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6922-6943" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7452-7475" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7452-7475" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7452-7475" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7452-7475" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="18" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="19" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7638-7669" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7638-7669" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7638-7669" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7638-7669" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="20" name="P575 Pbfr_1R" sequence="ATATCATATGgttacctccattatttaaaactctaatc" description="Contains NdeI site"><BindingSite location="4551-4584" boundStrand="1" annealedBases="CATATgttacctccattatttaaaactctaatc" meltingTemperature="52"><Component bases="ATAT"/><Component hybridizedRange="4580-4584" bases="CATAT"/><Component bases="G"/><Component hybridizedRange="4551-4578" bases="gttacctccattatttaaaactctaatc"/></BindingSite><BindingSite simplified="1" location="4551-4584" boundStrand="1" annealedBases="CATATgttacctccattatttaaaactctaatc" meltingTemperature="52"><Component bases="ATAT"/><Component hybridizedRange="4580-4584" bases="CATAT"/><Component bases="G"/><Component hybridizedRange="4551-4578" bases="gttacctccattatttaaaactctaatc"/></BindingSite></Primer><Primer recentID="21" name="P296 Pbfr_R" sequence="atatGGTACCaaaatttaattctttataagttatcaatttag" description="0"><BindingSite location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatGGTACC"/><Component hybridizedRange="4459-4490" bases="aaaatttaattctttataagttatcaatttag"/></BindingSite><BindingSite simplified="1" location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatGGTACC"/><Component hybridizedRange="4459-4490" bases="aaaatttaattctttataagttatcaatttag"/></BindingSite></Primer><Primer recentID="22" name="P576 Pbfr_2R" sequence="ATATCATATGttacctcctgttacctccattatttaaaactctaatc" description="&quot;Contains NdeI site, adds optimal RBS&quot;"><BindingSite location="4551-4579" boundStrand="1" annealedBases="tgttacctccattatttaaaactctaatc" meltingTemperature="53"><Component bases="ATATCATATGttacctcc"/><Component hybridizedRange="4551-4579" bases="tgttacctccattatttaaaactctaatc"/></BindingSite><BindingSite simplified="1" location="4551-4579" boundStrand="1" annealedBases="tgttacctccattatttaaaactctaatc" meltingTemperature="53"><Component bases="ATATCATATGttacctcc"/><Component hybridizedRange="4551-4579" bases="tgttacctccattatttaaaactctaatc"/></BindingSite></Primer><Primer recentID="23" name="P546 Pbfr-R" sequence="atatCATATGttacctcctaaaatttaattctttataagttatcaatttag" description="&quot;Contains NdeI site, adds optimal RBS&quot;"><BindingSite location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatCATATGttacctcct"/><Component hybridizedRange="4459-4490" bases="aaaatttaattctttataagttatcaatttag"/></BindingSite><BindingSite simplified="1" location="4459-4490" boundStrand="1" annealedBases="aaaatttaattctttataagttatcaatttag" meltingTemperature="50"><Component bases="atatCATATGttacctcct"/><Component hybridizedRange="4459-4490" bases="aaaatttaattctttataagttatcaatttag"/></BindingSite></Primer><Primer recentID="24" name="KROL320 Bfr_UTR_r" sequence="CGTAATCATGGCaagttccatattgttacctccattattt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-02-04T17:01:06Z"><BindingSite location="4563-4602" boundStrand="1" annealedBases="CGTAATCATGGCaagttccatattgttacctccattattt" meltingTemperature="63"><Component hybridizedRange="4563-4602" bases="CGTAATCATGGCaagttccatattgttacctccattattt"/></BindingSite><BindingSite simplified="1" location="4563-4602" boundStrand="1" annealedBases="CGTAATCATGGCaagttccatattgttacctccattattt" meltingTemperature="63"><Component hybridizedRange="4563-4602" bases="CGTAATCATGGCaagttccatattgttacctccattattt"/></BindingSite></Primer><Primer recentID="25" name="KROL321  Bfr_UTR_OE_f" sequence="aatatggaacttGCCATGATTACGGATTCACTGGCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-02-04T17:03:25Z"><BindingSite location="4579-4614" boundStrand="0" annealedBases="aatatggaacttGCCATGATTACGGATTCACTGGCC" meltingTemperature="66"><Component hybridizedRange="4579-4614" bases="aatatggaacttGCCATGATTACGGATTCACTGGCC"/></BindingSite><BindingSite simplified="1" location="4579-4614" boundStrand="0" annealedBases="aatatggaacttGCCATGATTACGGATTCACTGGCC" meltingTemperature="66"><Component hybridizedRange="4579-4614" bases="aatatggaacttGCCATGATTACGGATTCACTGGCC"/></BindingSite></Primer><Primer recentID="26" name="KROL395  lacZ_qPCR_F1" sequence="ATTCACTGGCCGTCGTTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T00:48:51Z"><BindingSite location="4604-4621" boundStrand="0" annealedBases="ATTCACTGGCCGTCGTTT" meltingTemperature="57"><Component hybridizedRange="4604-4621" bases="ATTCACTGGCCGTCGTTT"/></BindingSite><BindingSite simplified="1" location="4604-4621" boundStrand="0" annealedBases="ATTCACTGGCCGTCGTTT" meltingTemperature="57"><Component hybridizedRange="4604-4621" bases="ATTCACTGGCCGTCGTTT"/></BindingSite></Primer><Primer recentID="27" name="KROL396  lacZ_qPCR_R1" sequence="TGTGCTGCAAGGCGATTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T00:50:16Z"><BindingSite location="4662-4679" boundStrand="1" annealedBases="TGTGCTGCAAGGCGATTA" meltingTemperature="56"><Component hybridizedRange="4662-4679" bases="TGTGCTGCAAGGCGATTA"/></BindingSite><BindingSite simplified="1" location="4662-4679" boundStrand="1" annealedBases="TGTGCTGCAAGGCGATTA" meltingTemperature="56"><Component hybridizedRange="4662-4679" bases="TGTGCTGCAAGGCGATTA"/></BindingSite></Primer><Primer recentID="28" name="KROL397  lacZ_qPCR_F2" sequence="CCTGGCGTTACCCAACTTAATC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T01:02:38Z"><BindingSite location="4645-4666" boundStrand="0" annealedBases="CCTGGCGTTACCCAACTTAATC" meltingTemperature="57"><Component hybridizedRange="4645-4666" bases="CCTGGCGTTACCCAACTTAATC"/></BindingSite><BindingSite simplified="1" location="4645-4666" boundStrand="0" annealedBases="CCTGGCGTTACCCAACTTAATC" meltingTemperature="57"><Component hybridizedRange="4645-4666" bases="CCTGGCGTTACCCAACTTAATC"/></BindingSite></Primer><Primer recentID="29" name="KROL398  lacZ_qPCR_R2" sequence="AGCGCCATTCGCCATTC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T01:03:24Z"><BindingSite location="1806-1821" boundStrand="1" annealedBases="GCGCCATTCGCCATTC" meltingTemperature="57"><Component bases="A"/><Component hybridizedRange="1806-1821" bases="GCGCCATTCGCCATTC"/></BindingSite><BindingSite location="4752-4768" boundStrand="1" annealedBases="AGCGCCATTCGCCATTC" meltingTemperature="59"><Component hybridizedRange="4752-4768" bases="AGCGCCATTCGCCATTC"/></BindingSite><BindingSite simplified="1" location="1806-1821" boundStrand="1" annealedBases="GCGCCATTCGCCATTC" meltingTemperature="57"><Component bases="A"/><Component hybridizedRange="1806-1821" bases="GCGCCATTCGCCATTC"/></BindingSite><BindingSite simplified="1" location="4752-4768" boundStrand="1" annealedBases="AGCGCCATTCGCCATTC" meltingTemperature="59"><Component hybridizedRange="4752-4768" bases="AGCGCCATTCGCCATTC"/></BindingSite></Primer><Primer recentID="31" name="Primer 1" sequence="CGGGCCTCTTCGCTATTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T01:05:37Z"><BindingSite location="1754-1772" boundStrand="1" annealedBases="CGGGCCTCTTCGCTATTAC" meltingTemperature="56"><Component hybridizedRange="1754-1772" bases="CGGGCCTCTTCGCTATTAC"/></BindingSite><BindingSite location="4700-4718" boundStrand="1" annealedBases="CGGGCCTCTTCGCTATTAC" meltingTemperature="56"><Component hybridizedRange="4700-4718" bases="CGGGCCTCTTCGCTATTAC"/></BindingSite><BindingSite simplified="1" location="1754-1772" boundStrand="1" annealedBases="CGGGCCTCTTCGCTATTAC" meltingTemperature="56"><Component hybridizedRange="1754-1772" bases="CGGGCCTCTTCGCTATTAC"/></BindingSite><BindingSite simplified="1" location="4700-4718" boundStrand="1" annealedBases="CGGGCCTCTTCGCTATTAC" meltingTemperature="56"><Component hybridizedRange="4700-4718" bases="CGGGCCTCTTCGCTATTAC"/></BindingSite></Primer><Primer recentID="30" name="KROL399  lacZ_qPCR_F3" sequence="CCAACGTGACCTATCCCATTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T01:07:55Z"><BindingSite location="4892-4913" boundStrand="0" annealedBases="CCAACGTGACCTATCCCATTAC" meltingTemperature="56"><Component hybridizedRange="4892-4913" bases="CCAACGTGACCTATCCCATTAC"/></BindingSite><BindingSite simplified="1" location="4892-4913" boundStrand="0" annealedBases="CCAACGTGACCTATCCCATTAC" meltingTemperature="56"><Component hybridizedRange="4892-4913" bases="CCAACGTGACCTATCCCATTAC"/></BindingSite></Primer><Primer recentID="32" name="KROL400  lacZ_qPCR_R3" sequence="TTCCTGTAGCCAGCTTTCATC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-01-12T01:08:25Z"><BindingSite location="4978-4998" boundStrand="1" annealedBases="TTCCTGTAGCCAGCTTTCATC" meltingTemperature="57"><Component hybridizedRange="4978-4998" bases="TTCCTGTAGCCAGCTTTCATC"/></BindingSite><BindingSite simplified="1" location="4978-4998" boundStrand="1" annealedBases="TTCCTGTAGCCAGCTTTCATC" meltingTemperature="57"><Component hybridizedRange="4978-4998" bases="TTCCTGTAGCCAGCTTTCATC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="1:8:25">2021.1.12</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unique 6+ Cutters                                                                                            d      HO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
