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d}#T]#   ,b0	9	n	Mz[MRfl? W  ,NwH:3+_ - aK	<
N
qwn_5 (]*	
,
~<L(  DYi4H]qv>	y		O9|B^n#gxx C Fsw
{Dx /y3 -g	
V	6WhW)!WAc*{b  1n6 		

#Q+(Z'n   }

%./]d+W]'~  7 	{
v*+P`bLLD $~
lBxe%Bv	C   +NMv/	8	m			/
y+"t" B  Ev)		N8m}f^ 0z

"gVcY_ aKVb&&  #M
(	.		
MkfbN ' (           i[\	-	
Wj"#%&=}
"#<Y	y%XYZ[5      	`Q=mBKab'>X~$=$ OE\, )  Gj]	
X$mZ{
z&% 6 
2Rj/=b		


(Y18DF*    :C:7c?%->fP_L    a>L4
3eKby2lW]  Y.bnb%%!' AH 		n


:%.]['' * T   gkB^d	T


0 |&  %;Y		`

	,Y5GHIE;	'O  9mG


*naq{3 jz M3~#P>"


AZ29=x9FG)- 4F'7V5			'
K

aEH^?~SV 7 sk0c	
^r3nhUnjc  nISO!m2	8

)M [t(8@&*  % R 	W,;IY#8;E
R
K +`%`M  g  2;x!5DNBD.?T\^g`Q' u  	
F]dOhAg  (?U~	}&OUiY/+   ? ]z	FMs	5
4i9cz3"bLXr3^6 H n y   t4!J|/h(B"t| 	S_fl3V6-KezKm .=bqdo?	wLX  oPU	r

, SOt  -;	
(0&Aq  i  Zcd7c_	
pAtR&"6R$ 9`/p		|o%.*/+  B
<^pJLsV\q: *%
H	o



;	([9 IJ	
p+ 3		n&/-O<r*e a! + U l_e	
*
(U! y$}    !C9`l


1 ;{"t # w hWS	
\a  ! Y UG	U

&B A:QC( [ Z&+Z	W
<-HfIJ[<849	P 3<	
j(   PyV		a
e
[HZ
E  ?SzW	

 >6F;<p1  j:Iz


DCF\r^1I! *W
H


X52_Q+S	;>ouI  y.>Q| n	obi6 {A	M	

4ELf4:ZN**  kJ(\	>B3}U[k][{>6CgH
A* N$Q?#		w(FW56Xyg:G1{; 48R	#

sJDO3:g>~=Rhj4"  5Wu			L
K[b{`.  @8&	6	

wI/d9W_k@W\> l rDG%*3M-k6	P

QuZ;FISJTxKV    (		(
j

y9G 8 l:d	{2Uo4@V  

sXRod 2 N   =flw	_s4/B : a tnCW1/hi%k[  \"<fY
`i&pq{0dzBN$   JTAfTE	9

R#Z>91P27$ / ] OoPcf	3		>
LWHS  `n	A

"*B\7|ha;U Rl
 q )soD  |aG9 LQe* G\up{A  ~ |5
$u'  } A  *uLuyl	.
!dl|JaB$  &   _
<lJZ,		LVi	!; Nz
W" 5 1X-ai.9<6d	,axC^  S 
$F
S


+FD&pN 3y"6O	7
`qT>R(  Q wH'CE]_f-  > <y'Er>Tg}'A!s{			|k 8U5had"  h  8O	n/@+$*N 2
;oKqpZo` v  8_R		AT9#B >R
d
=GeiZ8 
1^Phnt5  @		
'2D}g0z+     $?)Lt			
PHY:8VjF M `   Vkv.Z%  N_@		
Q~!KV%A6zgO1G p {`FK~nPd+ 4 @  ^khtKtx-	5 Uchc:{
CBMoAV+   P v7GNS|&z	6
{mj7p4#c% Q
71Q	
c
<:n"oY7s4_, L    #^Kju		
  JUX@9f|NE, o u66JR_s{%y	z;JTbg9bm6	A!n@U I J K        5"][\}~i)#$X}~			{			


`a:;78IT`a45W78{LMD/  F]0*CBY|		<dIN.Lo" Z}	.  />pG"1nD\NOMi   e ^@+C		g
]maxMu" c%r		(l=r@eJw/p.u#Y [	s
eHjkjP E  RSpvyV	'T u
 
}-/% Q~		
 =Fmju( q2o[M<		1O'NjBzr]J  ?.b	)$ 0|HY3
@]1P1r   Uhx#1E	

Zc+ j HdA8di	h
^yS#2e7S~ <   _pEe"qnBv){  L#[ D
8bNus f,Y`			' 
 c &:>m>K q&/ ds	0q$Z A0.		q	}jAfx+.> a	)sp /&0,v Ce\	_  lj
t

H%8_|IT $>i			=Yk2l6uYPg( [
,fo2MIqsKMtFW*]kr;Q  S+I	
vu)Uy*  @W		|
@8Fv60#(C$}. F e [^Tq zp

r+f(^l# (w
<HD
O\/7: 
!Y}.yeh </	ym 	
 kAL~e,>  Jv<K 0$qH, Yh	0.Sssuw"f! R4Kq	X(3v3  	
b	o/=c>UP1=HG@On`Kk8 			
j'E2+t3>dbg! uNT!u
%{s:" V +		
2SZS*k\>^- , rCh`3p \=	

+
;
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a<|#  a	
2ekK%  @f8:c		_
m



/4-\` iL}4&
J


]2wb $  1:VX"ZMs	D

A*& x 3pcI%^2!Xq2 ,6T	
Q-.5Rps  $G	
mN;rU)9d    " TVF

%'[!@s Z Yy2VU		V
Z:3Do%  ViH	


CPBR[=HA 7I
'"	[	=rvICNKEfje\395BQfY	M@7 k qC2,j5	i

Jt.zcRI8JU_
 1  <eB9	
.gz1TW3?Q$A+ \ [ee	@
X
_h=c0:TQk    {4F#roC)|  	`
 kw = q=f r		_*) 0@SJm _     Ms		
1F 0E
b
On~6U'  \QzW	
I96HSc?@ $!	f
'\k[Mlqv--t QR	
d	G
<  Tg{0Z	a		
?Yb#q "Xh!7(d @--p= dk	
+n$L^5f*  d '?	{
?Eu 
"G'N]k#.x  ;;	xdlG6 XJp	
.RDr0G1N!2s_2=Jf' t}		
-)R)t
?v[v9 e	^

63`d~J 9+bC
I

1,-W%p  X
Y	l9qTZr(<n(  B;1	
	
Bgs-DybP/@?j zq?R" /	r	
j~k5GR,}5T !/c"$ c		*t-	!04jc1-kwFe 	,p'8 piri bo	q,j7d   zTTTN   {TTNT   |TNTA   }NTAG  TCAN  CANG  ANGC  NGCC  TGAN  GANT  ANTC  NTCA  &CGAN  'GANG  (ANGC  )NGCA  /  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                         &                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      	                                                                                                                                                                                                                                                           #                                        	                                           !                                                                                                                      	            7      
               	      	                  	                         
                                             ,   6   /   
      2      %            t   W      $            +   Q                                                                                                                                                                    &                                                                                                                                                                                                                                                                                                            	                                                                                                 #              	               !                                          	    7  
     	  	      	         
               , 6 / 
  2  %    t W  $    + Q         @  @    tOxq;x5G,-Pqc[V C	g		
h,uD	L
Lh(,5O[#u"c? aD

?>X	Z <%q'sPy^?q
7	xs>> P GIue&
NZzF_	
[
qW  `JV5	cMI	\eaSD		"*6SD	"6Qcw4SD	"6E*CoG	rs J8e
Sh;a>bbk
>NZzJBFj\@s	#p
bcqTd8oj

et,dXL8=0	p
D\/gmSD		"*6CE3\ae"s6|q(M
eMat	PTqTdB\ab]	n	nzPes:
3Z YV
c	TgF6GR*f 5	j\c@MPJV IS1M&uBu5SD	:"B8 y6	e   AH0#3)sx e,ved&f	.0NJ\dGQe\uQs	# Cf'y
		A	:]
X~ F
N ]	
n&?P_o	{)t	BDt?9?
)(lx
Vg	cd	*4$	uvZUh	
x
V)(l?
+,-!
@<8EpJLsV\q:wWo	?
Z
1)QrE)M (l<cdt		*BDt4?95
4Qo mHN l1	
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recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7927" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7598" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7621-7718" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7909" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4464" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="61" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7568" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4773-4796" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5565-5594" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6447-6471" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="66" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7091-7115" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="67" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="68" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4497-7568" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="69" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4514-4530" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="72" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="73" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#00e600" type="standard"/></Feature><Feature recentID="74" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7568" maxFusedRunOn="7568"><Segment range="4488-4496" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="75" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#a6acb3" type="standard"/></Feature><Feature recentID="76" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="77" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="81" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="82" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  7<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4530-4549" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4530-4549" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4530-4549" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4530-4549" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7542-7567" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7542-7567" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7542-7567" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7542-7567" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4733-4754" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4733-4754" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4733-4754" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4733-4754" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5255-5279" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5255-5279" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5255-5279" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5255-5279" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5781-5801" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5781-5801" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5781-5801" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5781-5801" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6303-6325" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6303-6325" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6303-6325" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6303-6325" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6827-6848" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6827-6848" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6827-6848" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6827-6848" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7357-7380" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7357-7380" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7357-7380" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7357-7380" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4472-4517" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="62"><Component hybridizedRange="4472-4495" bases="ctaattaagtagacaatgatagca"/><Component bases="ATG"/><Component hybridizedRange="4496-4517" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4472-4517" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="62"><Component hybridizedRange="4472-4495" bases="ctaattaagtagacaatgatagca"/><Component bases="ATG"/><Component hybridizedRange="4496-4517" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" 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  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="18:1:53">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  8          	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFyRZRGXda*rx_^ϣM=82Y1D?7Ҍ`Ń:STH/WF7
q\[*+[{;vTACZj){n p3Aʏ.LکE_>!8)mbL6r죍um1أΥ88Rb0ģ!]*`!\г>0i <lw\W^ L[n
<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"YA퍆l5;LFUC>jSʔUi.NYUs_+սBx]NӖ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7624-7721" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7818-7912" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4464" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="61" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-7571" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4776-4799" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5568-5597" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6450-6474" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="66" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7094-7118" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="67" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7571" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="68" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4497-7571" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="69" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4517-4533" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7571" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7571" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="72" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="73" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#00e600" type="standard"/></Feature><Feature recentID="74" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7571" maxFusedRunOn="7571"><Segment range="4488-4496" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="75" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#a6acb3" type="standard"/></Feature><Feature recentID="76" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="77" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="81" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="82" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  6<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4533-4552" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4533-4552" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4533-4552" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4533-4552" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7545-7570" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7545-7570" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7545-7570" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7545-7570" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4736-4757" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4736-4757" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4736-4757" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4736-4757" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5258-5282" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5258-5282" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5258-5282" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5258-5282" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5784-5804" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5784-5804" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5784-5804" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5784-5804" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6306-6328" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6306-6328" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6306-6328" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6306-6328" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6830-6851" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6830-6851" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6830-6851" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6830-6851" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7360-7383" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7360-7383" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7360-7383" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7360-7383" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4472-4520" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4472-4520" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4472-4520" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4472-4520" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7546-7577" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7546-7577" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7546-7577" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7546-7577" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="18:1:45">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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)ږ(aSG1i25=K*<Z3j3|084Q0a0گ!˲6WǝմƯb{:jxJш	t@sBL7 -rr_x3oŻ#(^7|s4SB0hU8\5<MжG#Au\3ͦ}	1>& =fp#ˇL\s1[!ೀ&ո?aC}́O<VUX:ᾣs1K¬gC*IʝVa\5ç!g<\l&<|oLMW}4Ҫ*oW~+d\N3SL!H;BWJ<3׸;t#_1`SݴσM ˃_s2o͸}`jlNYL֥U]x8m+#|eh2Rh>0=M24Fw0q26_WH-X1H11o^=83BMWiE^oϧ>*GbgLU8s1hZ`H3zvt^n8E8Y¯f h l0Z3}҂Ue6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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hitsStopCodon="1"><Segment range="4372-7446" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="76" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4392-4408" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="77" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4372-7446" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="78" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4372-7446" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="79" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4363-4371" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="80" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4363-4371" color="#00e600" type="standard"/></Feature><Feature recentID="81" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7446" maxFusedRunOn="7446"><Segment range="4363-4371" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="82" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4363-4371" color="#a6acb3" type="standard"/></Feature></Features>
  1r<?xml version="1.0"?><Primers recycledIDs="7,8,9,10,13,14,15" nextValidID="26"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="11" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="12" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="16" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4408-4427" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4408-4427" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4408-4427" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4408-4427" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="17" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7420-7445" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7420-7445" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7420-7445" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7420-7445" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="18" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4611-4632" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4611-4632" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4611-4632" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4611-4632" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="19" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5133-5157" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5133-5157" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5133-5157" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5133-5157" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="20" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5659-5679" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5659-5679" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5659-5679" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5659-5679" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="21" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6181-6203" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6181-6203" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6181-6203" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6181-6203" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="22" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6705-6726" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6705-6726" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6705-6726" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6705-6726" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="23" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7235-7258" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7235-7258" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7235-7258" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7235-7258" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="24" name="Primer XXXX f2" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4347-4395" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4347-4395" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4347-4395" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4347-4395" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="25" name="Primer XXX r1" sequence="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T21:48:04Z"><BindingSite location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite><BindingSite simplified="1" location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite simplified="1" location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="2:36:3">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
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>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfy)$uM`pkx<,Ul4ݿӃ%C|l](>X3*E0Nn,x4cp۝~oppEp(jXNt=Ŧ2ʶ8
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-&*t:ˀxK8iYp01qҸ\.sͽ35]JҼ_lRLY-q;x 	O850#,u	](h^>4D^s$|\͂Ov>4.~!<=68	e2ZKCtbUu<1HS͡\80C4(7ގ8i4@~O_	 `Ɵ(b wͼ3x	4L	^5y><8-r1Wši} 1ҵO9Mx`W=s7`f
{OIkӄ9J-UHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ]<?xml version="1.0"?><Features nextValidID="83"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7595-7952" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7595-7623" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning 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terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4485" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="61" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4485" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="62" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4485" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="63" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="64" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="65" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="66" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4509" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="67" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4486" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="68" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4509" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7589-7593" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="70" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4798-4821" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="71" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5590-5619" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="72" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6472-6496" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="73" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7140" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="74" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4519-7593" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="75" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4519-7593" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="76" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4539-4555" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="77" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4519-7593" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="78" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4519-7593" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="79" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4518" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="80" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4510-4518" color="#00e600" type="standard"/></Feature><Feature recentID="81" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7593" maxFusedRunOn="7593"><Segment range="4510-4518" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="82" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4518" color="#a6acb3" type="standard"/></Feature></Features>
  K|<?xml version="1.0"?><Primers nextValidID="26"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="8" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="9" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="10" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="11" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="12" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="13" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="16" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4555-4574" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4555-4574" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4555-4574" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4555-4574" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="17" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7567-7592" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7567-7592" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7567-7592" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7567-7592" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="18" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4758-4779" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4758-4779" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4758-4779" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4758-4779" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="19" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5280-5304" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5280-5304" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5280-5304" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5280-5304" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="20" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5806-5826" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5806-5826" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5806-5826" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5806-5826" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="21" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6328-6350" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6328-6350" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6328-6350" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6328-6350" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="22" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6852-6873" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6852-6873" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6852-6873" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6852-6873" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="23" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7382-7405" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7382-7405" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7382-7405" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7382-7405" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="24" name="Primer XXXX f2" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4494-4542" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4494-4542" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4494-4542" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4494-4542" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="25" name="Primer XXX r1" sequence="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T21:48:04Z"><BindingSite location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite><BindingSite location="4454-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="58"><Component hybridizedRange="4454-4502" bases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite location="4455-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaattt" meltingTemperature="52"><Component hybridizedRange="4465-4502" bases="cttaattagaacataactagtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="4459-4464" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="4455-4457" bases="ttt"/></BindingSite><BindingSite simplified="1" location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite simplified="1" location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite><BindingSite simplified="1" location="4454-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="58"><Component hybridizedRange="4454-4502" bases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite simplified="1" location="4455-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaattt" meltingTemperature="52"><Component hybridizedRange="4465-4502" bases="cttaattagaacataactagtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="4459-4464" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="4455-4457" bases="ttt"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="2:35:51">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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)ږ(aSG1i25=K*<Z3j3|084Q0a0گ!˲6WǝմƯb{:jxJш	t@sBL7 -rr_x3oŻ#(^7|s4SB0hU8\5<MжG#Au\3ͦ}	1>& =fp#ˇL\s1[!ೀ&ո?aC}́O<VUX:ᾣs1K¬gC*IʝVa\5ç!g<\l&<|oLMW}4Ҫ*oW~+d\N3SL!H;BWJ<3׸;t#_1`SݴσM ˃_s2o͸}`jlNYL֥U]x8m+#|eh2Rh>0=M24Fw0q26_WH-X1H11o^=83BMWiE^oϧ>*GbgLU8s1hZ`H3zvt^n8E8Y¯f h l0Z3}҂Ue6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      .  3     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="76" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6609-6633" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="77" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7253-7277" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="78" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4656-7730" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="79" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4656-7730" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="80" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4676-4692" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="81" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4656-7730" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="82" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4656-7730" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="83" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4647-4655" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="84" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4647-4655" color="#00e600" type="standard"/></Feature><Feature recentID="85" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7730" maxFusedRunOn="7730"><Segment range="4647-4655" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="86" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4647-4655" color="#a6acb3" type="standard"/></Feature></Features>
  S<?xml version="1.0"?><Primers nextValidID="28"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="8" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="9" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="10" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="11" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="12" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="13" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="14" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="16" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="17" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4660" boundStrand="1" annealedBases="GGCCtgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGC"/><Component hybridizedRange="4657-4660" bases="GGCC"/><Component bases="GC"/><Component hybridizedRange="4627-4654" bases="tgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4627-4660" boundStrand="1" annealedBases="GGCCtgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGC"/><Component hybridizedRange="4657-4660" bases="GGCC"/><Component bases="GC"/><Component hybridizedRange="4627-4654" bases="tgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="18" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4692-4711" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4692-4711" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4692-4711" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4692-4711" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="19" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7704-7729" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7704-7729" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7704-7729" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7704-7729" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="20" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4895-4916" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4895-4916" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4895-4916" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4895-4916" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="21" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5417-5441" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5417-5441" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5417-5441" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5417-5441" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="22" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5943-5963" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5943-5963" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5943-5963" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5943-5963" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="23" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6465-6487" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6465-6487" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6465-6487" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6465-6487" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="24" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6989-7010" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6989-7010" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6989-7010" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6989-7010" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="25" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7519-7542" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7519-7542" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7519-7542" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7519-7542" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="26" name="Primer XXXX f2" sequence="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4632-4679" boundStrand="0" annealedBases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4632-4679" bases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4632-4679" boundStrand="0" annealedBases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4632-4679" bases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="27" name="Primer XXXX r1" sequence="gtctacttaattagaacataactctgcacgacgaactaatactctatct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T21:00:15Z"><BindingSite location="4596-4644" boundStrand="1" annealedBases="gtctacttaattagaacataactctgcacgacgaactaatactctatct" meltingTemperature="64"><Component hybridizedRange="4596-4644" bases="gtctacttaattagaacataactctgcacgacgaactaatactctatct"/></BindingSite><BindingSite simplified="1" location="4596-4644" boundStrand="1" annealedBases="gtctacttaattagaacataactctgcacgacgaactaatactctatct" meltingTemperature="64"><Component hybridizedRange="4596-4644" bases="gtctacttaattagaacataactctgcacgacgaactaatactctatct"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="21:45:9">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       4     	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     f4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk/idI4JbiI`pkx<,Ul4ݿӃ%C|l](>X3*E0Nn,x4cp۝~oppEp(jXNt=Ŧ2ʶ8
p,jͪU#HpқFf j.8_wV=XaO3C+F#J2~p%Ү=03VL
c̷y˅l xOb¯1V8p2q66BM`oSEשs+6'sp`X[.o3a1s%X#LmHۂ X[C3s88V#I0O|f'32}?ÈX/mU`~G3Y].
-&*t:ˀxK8iYp01qҸ\.sͽ35]JҼ_lRLY-q;x 	O850#,u	](h^>4D^s$|\͂Ov>4.~!<=68	e2ZKCtbUu<1HS͡\80C4(7ގ8i4@~O_	 `Ɵ(b wͼ3x	4L	^5y><8-r1Wši} 1ҵO9Mx`W=s7`f
{OIkӄ9J-UHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i      %  *     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" 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recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7723-8080" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7723-7751" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7774-7871" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7968-8062" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4577-4622" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="co-chaperonin GroES" directionality="1" translationMW="1837.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4639" maxFusedRunOn="4639"><Segment range="4577-4622" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="63" name="P051 groESpromoR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4586" color="#0080ff" type="standard"/><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="64" name="P225 SP_p1715_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4588-4614" color="#8080ff" type="standard"/><Q name="note"><V text="P225 SP_p1715_R"/></Q></Feature><Feature recentID="65" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4521" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="66" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="67" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="68" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="70" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4623" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="72" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature><Feature recentID="73" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7717-7721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="74" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4926-4949" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="75" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5718-5747" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="76" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6600-6624" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="77" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7244-7268" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="78" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4647-7721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="79" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4647-7721" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="80" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4667-4683" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="81" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4647-7721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="82" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4647-7721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature></Features>
  Mc<?xml version="1.0"?><Primers nextValidID="26"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="11" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="1" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="3" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="4" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="5" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="6" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="13" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4648" boundStrand="1" annealedBases="cattgtctacttaattagaaca" meltingTemperature="48"><Component bases="atatGCGGCCGCtgctat"/><Component hybridizedRange="4627-4648" bases="cattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4627-4648" boundStrand="1" annealedBases="cattgtctacttaattagaaca" meltingTemperature="48"><Component bases="atatGCGGCCGCtgctat"/><Component hybridizedRange="4627-4648" bases="cattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="16" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4683-4702" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4683-4702" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4683-4702" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4683-4702" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="17" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7695-7720" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7695-7720" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7695-7720" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7695-7720" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="12" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4886-4907" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4886-4907" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4886-4907" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4886-4907" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="0" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5408-5432" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5408-5432" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5408-5432" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5408-5432" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="22" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5934-5954" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5934-5954" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5934-5954" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5934-5954" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="23" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6456-6478" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6456-6478" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6456-6478" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6456-6478" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="24" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6980-7001" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6980-7001" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6980-7001" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6980-7001" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="25" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7510-7533" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7510-7533" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7510-7533" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7510-7533" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:42:56">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       3     	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     c4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk/idI4JbiI6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      %  '     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5005" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-4676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4699-4796" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4893-4987" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4577-4622" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="co-chaperonin GroES" directionality="1" translationMW="1837.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4639" maxFusedRunOn="4639"><Segment range="4577-4622" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="63" name="P051 groESpromoR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4586" color="#0080ff" type="standard"/><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="64" name="P225 SP_p1715_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4588-4614" color="#8080ff" type="standard"/><Q name="note"><V text="P225 SP_p1715_R"/></Q></Feature><Feature recentID="65" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4521" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="66" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="67" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="68" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="70" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4623" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="72" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature></Features>
  :<?xml version="1.0"?><Primers recycledIDs="16,17,12,0" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="11" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="1" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="3" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="4" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="5" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="6" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="13" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4687" boundStrand="1" annealedBases="tGCGGCCtatcattgtctacttaattagaaca" meltingTemperature="44"><Component bases="ata"/><Component hybridizedRange="4681-4687" bases="tGCGGCC"/><Component bases="GCtgc"/><Component hybridizedRange="4668-4673" bases="tatcat"/><Component hybridizedRange="4627-4645" bases="tgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4627-4645" boundStrand="1" annealedBases="tgtctacttaattagaaca" meltingTemperature="44"><Component bases="atatGCGGCCGCtgctatcat"/><Component hybridizedRange="4627-4645" bases="tgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:41:25">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       -  u   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     K4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk/idI46<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          t   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4624-4652" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4675-4772" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4869-4963" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4577-4622" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="co-chaperonin GroES" directionality="1" translationMW="1837.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4633" maxFusedRunOn="4633"><Segment range="4577-4622" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="63" name="P051 groESpromoR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4586" color="#0080ff" type="standard"/><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="64" name="P225 SP_p1715_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4588-4614" color="#8080ff" type="standard"/><Q name="note"><V text="P225 SP_p1715_R"/></Q></Feature><Feature recentID="65" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4521" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="66" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="67" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="68" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature></Features>
  7<?xml version="1.0"?><Primers recycledIDs="15,16,17,12,0" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="11" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="1" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="3" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="4" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="5" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="6" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="13" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:38:57">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  U       Y   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     /4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          X   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  B/<?xml version="1.0"?><Features recycledIDs="59,60,61,62,63,64" nextValidID="65"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" 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  <?xml version="1.0"?><Primers recycledIDs="11,12,0,1,2,3,4,5,6,7,8,9,13,14,15,16,17" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:37:13">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  V       \   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     24~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>HNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i          [   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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consecutiveTranslationNumbering="1"><Segment range="4588-4682" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4339-4341" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="11,12,0,1,2,3,4,5,6,7,8,9,13,14,15,16,17" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:35:0">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  p   
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     !4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61D	QeU~fUSBm]EiWtQY9UN99UE5.<ٞXYBVPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S               J   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q 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color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4838-4866" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning 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recentID="62" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4594-4791" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="63" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4801-4833" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4594-4836" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  2R<?xml version="1.0"?><Primers recycledIDs="11,12" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL42 OE_rpsU3R" sequence="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4762-4802" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4762-4802" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="2" name="KROL41 OE_rpsU3F" sequence="atatGAATTCaggagggataatatgccaagaataatagttgacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4587-4614" boundStrand="0" annealedBases="gagggtatgccaagaataatagttgacc" meltingTemperature="52"><Component bases="atatGAATTCag"/><Component hybridizedRange="4587-4591" bases="gaggg"/><Component bases="ataa"/><Component hybridizedRange="4592-4614" bases="tatgccaagaataatagttgacc"/></BindingSite><BindingSite simplified="1" location="4592-4614" boundStrand="0" annealedBases="tatgccaagaataatagttgacc" meltingTemperature="52"><Component bases="atatGAATTCaggagggataa"/><Component hybridizedRange="4592-4614" bases="tatgccaagaataatagttgacc"/></BindingSite></Primer><Primer recentID="3" name="KROL290 rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" dateAdded="2019-12-19T21:43:09Z"><BindingSite location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="4" name="KROL291 VSVG_bamHI_r" sequence="atatggatcCTTATTTACCTAATCTATTCATT" dateAdded="2019-12-19T21:43:09Z"><BindingSite location="4814-4842" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="4814-4842" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite><BindingSite simplified="1" location="4814-4842" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="4814-4842" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite></Primer><Primer recentID="5" name="KROL296 rpsU3_p2_F" sequence="gtgagagggtatgccaagaataatagttgaccctaaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:44:40Z"><BindingSite location="4583-4619" boundStrand="0" annealedBases="gtgagagggtatgccaagaataatagttgaccctaaa" meltingTemperature="63"><Component hybridizedRange="4583-4619" bases="gtgagagggtatgccaagaataatagttgaccctaaa"/></BindingSite><BindingSite simplified="1" location="4583-4619" boundStrand="0" annealedBases="gtgagagggtatgccaagaataatagttgaccctaaa" meltingTemperature="63"><Component hybridizedRange="4583-4619" bases="gtgagagggtatgccaagaataatagttgaccctaaa"/></BindingSite></Primer><Primer recentID="6" name="KROL297 rpsU3_p2_R" sequence="ttcttggcataccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:45:37Z"><BindingSite location="4569-4602" boundStrand="1" annealedBases="ttcttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component hybridizedRange="4569-4602" bases="ttcttggcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4602" boundStrand="1" annealedBases="ttcttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component hybridizedRange="4569-4602" bases="ttcttggcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="7" name="KROL263 pKR38_FR1R" sequence="atatgcggccgcctaataagaataagaccttcttgcttcttttttag" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4759-4802" boundStrand="1" annealedBases="atatgcggccgcataagaataagaccttcttgcttcttttttag" meltingTemperature="62"><Component hybridizedRange="4791-4802" bases="atatgcggccgc"/><Component bases="cta"/><Component hybridizedRange="4759-4790" bases="ataagaataagaccttcttgcttcttttttag"/></BindingSite><BindingSite simplified="1" location="4759-4802" boundStrand="1" annealedBases="atatgcggccgcataagaataagaccttcttgcttcttttttag" meltingTemperature="62"><Component hybridizedRange="4791-4802" bases="atatgcggccgc"/><Component bases="cta"/><Component hybridizedRange="4759-4790" bases="ataagaataagaccttcttgcttcttttttag"/></BindingSite></Primer><Primer recentID="8" name="KROL42 OE_rpsU3Ra" sequence="atatGCGGCCGCataagaataagaccttcttgcttcttttt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4762-4802" boundStrand="1" annealedBases="atatGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="atatGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4762-4802" boundStrand="1" annealedBases="atatGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="atatGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="9" name="KROL214 ChaC-V_FR1_R" sequence="atatCAATTGaattcttatttacctaatctattcatt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4814-4836" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="4814-4836" bases="cttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="4814-4836" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="4814-4836" bases="cttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="13" name="KROL120 ?rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4579-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcg" meltingTemperature="62"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="4579-4600" bases="cttggcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="4579-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcg" meltingTemperature="62"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="4579-4600" bases="cttggcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="14" name="KROL293 rpsU1_p2_R" sequence="tacttagcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4569-4600" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="ta"/><Component hybridizedRange="4598-4600" bases="ctt"/><Component bases="a"/><Component hybridizedRange="4569-4596" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4600" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="ta"/><Component hybridizedRange="4598-4600" bases="ctt"/><Component bases="a"/><Component hybridizedRange="4569-4596" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="15" name="KROL295 rpsU2_p2_R" sequence="cgcttggcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4569-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component bases="cg"/><Component hybridizedRange="4569-4600" bases="cttggcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component bases="cg"/><Component hybridizedRange="4569-4600" bases="cttggcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="16" name="KROL164 P035 INT_VSVG" sequence="TTTACCTAATCTATTCATTTCAATATCAG" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4804-4832" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="4804-4832" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite><BindingSite simplified="1" location="4804-4832" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="4804-4832" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite></Primer><Primer recentID="17" name="KROL240 katg-ck-1" sequence="gattacgaattcttatttacctaatctat" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4819-4836" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="4819-4836" bases="cttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="4819-4836" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="4819-4836" bases="cttatttacctaatctat"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:34:51">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y   	  o  \   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     24~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61DkAcYdU_Ej@TЛWQzDdtrUEVNUSi%UQMEc6ghVFHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i              [   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-4883" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning 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terminator"/></Q></Feature><Feature recentID="59" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature recentID="60" name="30S ribosomal protein S21" directionality="1" translationMW="7816.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4853" maxFusedRunOn="4853"><Segment range="4611-4808" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="61" name="rpsU3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4611-4808" color="#00e600" type="standard"/></Feature><Feature 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  <?xml version="1.0"?><Primers recycledIDs="1,2" nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="3" name="KROL42 OE_rpsU3R" sequence="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4779-4819" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4779-4819" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4779-4819" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4779-4819" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="4" name="KROL41 OE_rpsU3F" sequence="atatGAATTCaggagggataatatgccaagaataatagttgacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4631" boundStrand="0" annealedBases="GAATTCaggagggataatatgccaagaataatagttgacc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="4592-4631" bases="GAATTCaggagggataatatgccaagaataatagttgacc"/></BindingSite><BindingSite simplified="1" location="4592-4631" boundStrand="0" annealedBases="GAATTCaggagggataatatgccaagaataatagttgacc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="4592-4631" bases="GAATTCaggagggataatatgccaagaataatagttgacc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="21:42:47">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  \     n  X   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     .4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61DkA	S)~bjUCd,zSנ75QUW8Q;eYJU6ghVFHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i              W   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" 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recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4851-5208" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4851-4879" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4902-4999" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5096-5190" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional 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text="VSV-G"/></Q></Feature><Feature recentID="62" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="4569-4598" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="4569-4598" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4598" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="4569-4598" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="3" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4775-4815" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="4775-4815" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="4775-4815" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="4775-4815" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="4" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4628" boundStrand="0" annealedBases="GAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4592-4628" bases="GAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="4592-4628" boundStrand="0" annealedBases="GAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4592-4628" bases="GAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:4:46">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Hr        }   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     S4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:AkO6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="31" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="32" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="33" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="34" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="35" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="36" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="37" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="38" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="48" name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="49" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="50" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="51" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="52" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="53" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="54" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="55" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="56" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>97da295a-ac06-4ba6-951b-43fb4ef45dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="4:0:0">2016.10.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
        _          f	ٕ_Mn镝%)f$RyZy嘤YoyhVh$@v\WjS@         -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="1"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-264" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  A<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="0-39" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component hybridizedRange="0-39" bases="atatggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component hybridizedRange="0-39" bases="atatggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="241-273" boundStrand="1" annealedBases="atatgaattcccctctcacctcgcttaaatgcc" meltingTemperature="64"><Component hybridizedRange="241-273" bases="atatgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="241-273" boundStrand="1" annealedBases="atatgaattcccctctcacctcgcttaaatgcc" meltingTemperature="64"><Component hybridizedRange="241-273" bases="atatgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>60404d3f-5659-4a91-8a87-2be68693a367</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:25:13">2019.12.19</Created>
<LastModified UTC="16:25:13">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       u  *        *Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf@2iQQ,FQ1T8I^rgU=zsUUxUQ5dP0fxfadi	YAVfz|}+	U]=TU^wNDhsUhXzdl쑤Nix(Wz}YQSUFTӕ]EG)5;9MY.k45GU	)^Sh7GONtUl:.PU[iAIk[YSPƮ>CS5i1jTR>Jc[TMlNNP Q[]ETD$ЕZ1Te55vǳTM+`Gc&;QitV]M9w5S]GSaJB$EK^zCFUMfNdQTT$l{jem9)[mZn*V滪[+cWϙ:wiZIRNKEb}Zz;jD%d!b^f)\yncQz^fuTOI%V旮UY[ՂNk૪媩eWjhZԛmfؕYvYѹޗuKF:Ƃb[ITꎕ9yxf[bamUf񖆖#Y骕jn(^	[UٶgYmyM㢳RReYꕅY%%I(b X}a^ZT:_JNnnNm~!^n_hC^Qf+9uiMکz-fukWi%]yeURJuTZRRJkUHgUI*U#S5iUUufeDY@UoEy''`jFתEaykX]UmyUQM(rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]V9PSܑSEpabӞ\Q4$`NG©Sh(?RWPn3@QmDtAh]F
:CØxG[;<+ËL+Q#X^4CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H    (  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="14"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7466" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4716-4765" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="810-842" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="971-993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="4" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="5" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5666-6523" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="6" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4854-5645" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="7" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2659-3675" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="8" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2653" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="9" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7840.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="845" maxFusedRunOn="845"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="16:32:46">2019.1.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:52:23">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
 <?xml version="1.0"?><HistoryTree><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="7926" ID="21" circular="1" operation="remove"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4463..5452" colors="blue-black"/><ColorRange range="3473..4462" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4463..5452" colors="blue-black"/><ColorRange range="3473..4462" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4463" val2="4463"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="7927" ID="20" circular="1" resurrectable="1" operation="remove"><Features><Feature name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0"><Segment range="4497-7568" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7570-7927" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4464..5453" colors="blue-black"/><ColorRange range="3473..4462" colors="black-red"/><ColorRange range="4463..4463" colors="white"/></TopStrand><BottomStrand><ColorRange range="4464..5453" colors="blue-black"/><ColorRange range="3473..4462" colors="black-red"/><ColorRange range="4463..4463" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4496..5485" colors="blue-black"/><ColorRange range="3506..4495" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4496..5485" colors="blue-black"/><ColorRange range="3506..4495" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4496" val2="4498"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="7930" ID="19" circular="1" resurrectable="1" operation="insert"><Features><Feature name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0"><Segment range="4497-7571" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7573-7930" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4499..5489" colors="blue-black"/><ColorRange range="3505..4495" colors="black-red"/><ColorRange range="4496..4498" colors="white"/></TopStrand><BottomStrand><ColorRange range="4499..5489" colors="blue-black"/><ColorRange range="3505..4495" colors="black-red"/><ColorRange range="4496..4498" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4338..4462" colors="red"/></TopStrand><BottomStrand><ColorRange range="4338..4462" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4338"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="7805" ID="18" circular="1" resurrectable="1" operation="remove"><Features><Feature name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0"><Segment range="4372-7446" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7448-7805" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4362" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4363-4371" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4338..5312" colors="blue-black"/><ColorRange range="3363..4337" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4338..5312" colors="blue-black"/><ColorRange range="3363..4337" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4338" val2="4484"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="7952" ID="17" circular="1" resurrectable="1" operation="remove"><Features><Feature name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0"><Segment range="4519-7593" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7595-7952" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4339-4485" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4486-4509" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4510-4518" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4485..5478" colors="blue-black"/><ColorRange range="3344..4337" colors="black-red"/><ColorRange range="4338..4484" colors="white"/></TopStrand><BottomStrand><ColorRange range="4485..5478" colors="blue-black"/><ColorRange range="3344..4337" colors="black-red"/><ColorRange range="4338..4484" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4485..5478" colors="blue-black"/><ColorRange range="3491..4484" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4485..5478" colors="blue-black"/><ColorRange range="3491..4484" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4485" val2="4621"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="8089" ID="16" circular="1" resurrectable="1" operation="insert"><Features><Feature name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0"><Segment range="4656-7730" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7732-8089" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4647-4655" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4622..5632" colors="blue-black"/><ColorRange range="3474..4484" colors="black-red"/><ColorRange range="4485..4621" colors="white"/></TopStrand><BottomStrand><ColorRange range="4622..5632" colors="blue-black"/><ColorRange range="3474..4484" colors="black-red"/><ColorRange range="4485..4621" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4646..4654" colors="red"/></TopStrand><BottomStrand><ColorRange range="4646..4654" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4646"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="8080" ID="15" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0"><Segment range="4647-7721" color="#993366" type="standard"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7723-8080" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4646..7720" colors="red"/></TopStrand><BottomStrand><ColorRange range="4646..7720" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4646"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="5005" ID="14" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4648-5005" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4622..4645" colors="red"/></TopStrand><BottomStrand><ColorRange range="4622..4645" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4622"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="4981" ID="13" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4624-4981" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4338..4621" colors="red"/></TopStrand><BottomStrand><ColorRange range="4338..4621" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4338"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="4697" ID="12" circular="1" resurrectable="1" operation="remove" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4340-4697" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4338..227" colors="blue-black"/><ColorRange range="3751..4337" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4338..227" colors="blue-black"/><ColorRange range="3751..4337" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4338" val2="4340"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="4700" ID="11" circular="1" resurrectable="1" operation="remove" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4343-4700" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4341..227" colors="blue-black"/><ColorRange range="3751..4337" colors="black-red"/><ColorRange range="4338..4340" colors="white"/></TopStrand><BottomStrand><ColorRange range="4341..227" colors="blue-black"/><ColorRange range="3751..4337" colors="black-red"/><ColorRange range="4338..4340" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4338..224" colors="blue-black"/><ColorRange range="3751..4337" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4338..224" colors="blue-black"/><ColorRange range="3751..4337" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4338" val2="4832"/><Node name="pKR62 Tn7_pdpA_UTR_LacZ.dna" type="DNA" seqLen="5195" ID="10" circular="1" resurrectable="1" operation="replace" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4838-5195" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4593" color="#00ff00" type="standard"/></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="30S ribosomal protein S21" directionality="1" translationMW="7816.28" type="CDS" allowSegmentOverlaps="0"><Segment range="4594-4791" color="#ff0000" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPRIIVDPKKPFDISLRNFKRACEKAGIKQELRDRQHYVKPTQKRKIAKKAAISKAKKEARRSYSY"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4801-4833" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4833..286" colors="blue-black"/><ColorRange range="3689..4337" colors="black-red"/><ColorRange range="4338..4832" colors="white"/></TopStrand><BottomStrand><ColorRange range="4833..286" colors="blue-black"/><ColorRange range="3689..4337" colors="black-red"/><ColorRange range="4338..4832" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4592..4592" colors="red"/></TopStrand><BottomStrand><ColorRange range="4592..4592" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="4592" val2="4609"/><Node name="pKR61 Tn7_rpsU3-V.dna" type="DNA" seqLen="5212" ID="9" circular="1" resurrectable="1" operation="replace" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4855-5212" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4605-4808" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4818-4850" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4592..4609" colors="white"/></TopStrand><BottomStrand><ColorRange range="4592..4609" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4598..4852" colors="red"/></TopStrand><BottomStrand><ColorRange range="4598..4852" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="4598" val2="4848"/><Node name="pKR61 Tn7_rpsU3.dna" type="DNA" seqLen="5208" ID="8" circular="1" resurrectable="1" operation="insertFragments" hideHistory="1"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4851-5208" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0"><Segment range="4610-4804" color="#ff0000" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKR"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4814-4846" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><RegeneratedSite name="KpnI" pos="4337" siteCount="1"/><RegeneratedSite name="EcoRI" pos="4593" siteCount="1"/><RegeneratedSite name="BamHI" pos="4850" siteCount="1"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4598..4848" colors="white"/></TopStrand><BottomStrand><ColorRange range="4598..4848" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4337..4592" colors="red"/><ColorRange range="4593..4849" colors="blue"/></TopStrand><BottomStrand><ColorRange range="4333..4596" colors="red"/><ColorRange range="4597..4853" colors="blue"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" name1="BamHI" name2="KpnI" val1="4375" val2="4337" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="KpnI" name2="EcoRI" val1="9" val2="265" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="EcoRI" name2="BamHI" val1="589" val2="846" siteCount1="1" siteCount2="1"/><Node name="pMP749_(modified (1).dna" type="DNA" seqLen="4733" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4733" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4337..4374" colors="white"/></TopStrand><BottomStrand><ColorRange range="4333..4378" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node><Node name="pcr_rpsU2_promoter.dna" type="DNA" seqLen="274" ID="2" circular="0" upstreamModification="Unmodified" downstreamModification="Unmodified" resurrectable="1" operation="amplifyFragment" hideHistory="1"><Features><Feature name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0"><Segment range="11-264" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features><Oligo name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"/><Oligo name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"/><Parameter name="polymerase" val="polymerase that creates blunt ends"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="9..264" colors="red"/><ColorRange range="0..8" colors="white"/><ColorRange range="265..273" colors="white"/></TopStrand><BottomStrand><ColorRange range="5..268" colors="red"/><ColorRange range="0..4" colors="white"/><ColorRange range="269..273" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="0..9" colors="red"/><ColorRange range="264..273" colors="red"/></TopStrand><BottomStrand><ColorRange range="0..9" colors="red"/><ColorRange range="264..273" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="amplify" val1="1002702" val2="1002955"/><Node name="LVSgenomeWithPrimers.dna" type="DNA" seqLen="1895994" ID="1" circular="1" operation="invalid"><Primers><HybridizationParams minContinuousMatchLen="15" allowMismatch="0" minMeltingTemperature="40"/><Primer recentID="0" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1002702-1002731" boundStrand="0" annealedBases="aacctttaaccatataaatttctaaagaat" meltingTemperature="53"><Component bases="atatggtacc"/><Component hybridizedRange="1002702-1002731" bases="aacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="1" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1002933-1002955" boundStrand="1" annealedBases="ccctctcacctcgcttaaatgcc" meltingTemperature="61"><Component bases="atatgaattc"/><Component hybridizedRange="1002933-1002955" bases="ccctctcacctcgcttaaatgcc"/></BindingSite></Primer></Primers><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1002702..1002955" colors="black"/><ColorRange range="1002956..1002701" colors="white"/></TopStrand><BottomStrand><ColorRange range="1002702..1002955" colors="black"/><ColorRange range="1002956..1002701" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node><Node name="pKR7 pF-rpsU2-V.dna" type="DNA" seqLen="7466" ID="7" circular="1" resurrectable="1" operation="replace" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2659-3675" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1136-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5666-6523" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4854-5645" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2234-2653" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4716-4765" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="810-842" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="971-993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="589..845" colors="blue"/><ColorRange range="0..588" colors="white"/><ColorRange range="846..7465" colors="white"/></TopStrand><BottomStrand><ColorRange range="593..849" colors="blue"/><ColorRange range="0..592" colors="white"/><ColorRange range="850..7465" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="600..799" colors="red"/></TopStrand><BottomStrand><ColorRange range="600..799" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="600" val2="800"/><Node name="pKR7 pF-rpsU2-V.dna" type="DNA" seqLen="7467" ID="6" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="600..800" colors="white"/></TopStrand><BottomStrand><ColorRange range="600..800" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="594..599" colors="red"/></TopStrand><BottomStrand><ColorRange range="594..599" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="594"/><Node name="pKR6 pF-rpsU1-V.dna" type="DNA" seqLen="7461" ID="5" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="594..794" colors="red"/></TopStrand><BottomStrand><ColorRange range="594..794" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="594"/><Node name="pKR6 pF-rpsU1-V.dna" type="DNA" seqLen="7260" ID="4" circular="1" resurrectable="1" operation="remove" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="594..1500" colors="blue-black"/><ColorRange range="6947..593" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="594..1500" colors="blue-black"/><ColorRange range="6947..593" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="594" val2="1298"/><Node name="pKR6 pF-rpsU1-V.dna" type="DNA" seqLen="7965" ID="3" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0"><Segment range="616-1299" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MRILLAEDDLHLGEGLLEALQKEGLIVNLVSDGEAAQTFIESGLYDIVVLDIGMPIKTGLEVLRNIRNRGIKVPIILLTARDGLEDRIKGLDLGADDYLTKPFELKELVARIKAISRRIDTRSGKSVNEEIRFGDYSFNPSSETVTKDGVIIPVSKKELALLAILVQNAGRVVPKTQLLEEIYSTDKEMDTNTLEVHMHNLRKKINIPNFIQTIRGVGYFVQKDKVIK"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1299..2293" colors="blue-black"/><ColorRange range="7564..593" colors="black-red"/><ColorRange range="594..1298" colors="white"/></TopStrand><BottomStrand><ColorRange range="1299..2293" colors="blue-black"/><ColorRange range="7564..593" colors="black-red"/><ColorRange range="594..1298" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4487-4495" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="73" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4487-4495" color="#00e600" type="standard"/></Feature><Feature recentID="74" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7567" maxFusedRunOn="7567"><Segment range="4487-4495" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIA"/></Q></Feature><Feature recentID="75" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4487-4495" color="#a6acb3" type="standard"/></Feature><Feature recentID="76" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="77" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="81" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="82" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  6<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4529-4548" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4529-4548" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4529-4548" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4529-4548" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7541-7566" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7541-7566" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7541-7566" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7541-7566" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4732-4753" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4732-4753" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4732-4753" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4732-4753" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5254-5278" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5254-5278" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5254-5278" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5254-5278" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5780-5800" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5780-5800" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5780-5800" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5780-5800" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6302-6324" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6302-6324" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6302-6324" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6302-6324" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6826-6847" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6826-6847" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6826-6847" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6826-6847" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7356-7379" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7356-7379" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7356-7379" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7356-7379" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4468-4516" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4468-4516" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4468-4516" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4468-4516" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4479" boundStrand="1" annealedBases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7542-7573" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7542-7573" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7542-7573" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7542-7573" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="18:4:31">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                            d      HO  d               E             Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
