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j

y 8 l:d	{23  

s 2 N   =flw	_] : a tnCW1/h  \"<fY
`i   JTAfTE	9

R# / ] OoPcf	3		>
LW `n	A

"*u
 q )soz  |aG9 LRPV G\up{A@Y  ~ |5
$u| A  *uLuyl	.
!d9cG$  &   _
<lJZ,		LVi 0rdF  5 1X-ai.9<6d	LC S 
$F
S


+Fhn 3y"6O	7
`  Q wH'CE.>C(  > <y'Er>Tg}'A!s{			|kEn  h  8O	n'HA+ 2
;` v  8_R		AT4 >R
d
=Gew%" 
1^P  @		
'2D     $?)Lt			
Pq M `   Vkv\2  N_@		
Q~!KV p {`FK~nQO$ 4 @  ^khtKtx-	5 UchcbgF!   P v7GNS|&z	6
{mj&-=Gm Q
71Q	
c
<:v_!! L    #^Kju		
  JUp& o u66JR_s{%y	z;JTbg9bm%alI I J K        5"][\}~i)#$X}~			{			


`a:;78IT`a$_`k   F]0*CBY|		<d&Yf Z}	Zg />pG"1nD\N'3   e ^@+C		g
]max  c%r		(l=r@e
 [	s
e E  RSpvyV	[ u
 
 h Q~		
 q2o[M<		1O(>  ?.b	)(Cy- 0|HY3
@]  Uhx#1E	

Z4 j HdA8di	h
^y <   _pEe"qnJ)  L#[ D
8b+OW f,Y`		) 
 c &:>m> ds	7 A0.		q	}jAf" a	)sp Ce\	 lj
t

H%8 $>i			= [
,fo2  S+I	
  @W		|
@8FvK F e [^Tq zp

 (w
<8\ 
!Y </	yb 	
 k1i  Jv<K Yh	0 R4Kq	X
  	
b	o/ 			
j' uNT=)1 V +		
2SZoB , rCh`3p \=	

+
;
6T m~f	P).V*Z?  /D	7*Hz9   "
a<|)D;  a	
2  @f8:c		_
m



/4-j?. iL}4&
J


] $  1:VX"ZMs	D

A x 3pcI%^"]	 ,6T	
	  $G	
m    " TVF

%'[m Z Yy2VU		V
Z  ViH	


C5 7I
'"	[	=rvICNK$! k qC2,j5	i

Jt_ 1  <eB9	
.gz1 \ [ee	@
X
_h    {4F#roy~  	`
KmqB = q=f r		_* 0@S$ _     Ms		
1~1 0E
b
,PN  \QzW	
I96HScXe $!	f
'\k[Mlq QR	
d	  Tg{0Z	a		
?Y'x= "Xh!7*	 @- dk	
+n$  d '?	{
?Eu 7  ;;	x XJp	
.	 t}		
-)S e	^

63`i8 9+bC
I

  X
Y	l  B;1	
	
Bgs zq?Rl# /	r	
j~k5GRd !/w c		*t- 	,R pirk bo	q,   zTTTN   {TTNT   |TNTA   }NTAG  TCAN  CANG  ANGC  NGCC  TGAN  GANT  ANTC  NTCA  &CGAN  'GANG  (ANGC  )NGCA  (  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
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                                                                                          	            
   	      	               	                                                                                                        
                                                      K   J                  $   ?                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                        
                                  	    
 	  	     	                                    
                  K J      $ ?   `  a  a  a  d  g3  kwOxx5G,-V 	g	
hD	L
Lh(~A D

>X	Z 'P<q
7	x P G-Iu<
	
[
  `#5	MI	\SD		"SD	"Qc4JSD	"oG	s JRe
Shbk
>s	xp
`bcoj

,	p
SD		"3\"sq6|q(M}Jf\`b]	n	nz&:
Z YV
c	TgF6f 5	MP IS1Muf u5SD	:"f 2   AH0#3)s(d&f	.dQeuQs	x Cy
		A	]
~ F
N ]	
n&&_o	{t	B?
x
VgPcd	*$	uUh	
4x
V?
+!
@TwWo	?
Z
1cdt		*B5
 mHN l1	
s&Z	<HJ#	
Sl 
?Z	'=J_ + :_*
-f<5 ZE	JPN'_=, `;
Z YV
	- -nv4

 c j &>i	h
s
1>	jk}
AwPwP	


*Ex~7		x!sq# 4 w

x-nvWo	Z
1, Z Y`;
V
7
/G\
:Yh	0XJp	
.	#=ih		Z
G
17=^
  }

%./]}|;@Ig	aWi   {4F#roy~_c:	^	o	q


o{''V9\
g3EPPdk	
+n$iL}4&
J


]   7V59} 	{\



%gpt.Kv*/]3EP|/}Pb|;@I $ Q  13:iwyLV}"6HX"ZMOs'4		&
7
D
J



ACE`].>CIJ	
a  JTAfTE	9

R#   a>L4
3et$D!/wTv
DO4 @  ^khtKtx-	5 UchcbgF   Ivy}.*JO	


%t.C/>Q]>a}|;@IE  RSpvyV	[  Y   	  o  \   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     24~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61DkAcYdU_Ej@TЛWQzDdtrUEVNUSi%UQMEc6ghVFHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i              [   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  H<?xml version="1.0"?><Features nextValidID="65"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: 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range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" 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recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5212" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-4883" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4906-5003" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5100-5194" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature recentID="60" name="30S ribosomal protein S21" directionality="1" translationMW="7816.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4853" maxFusedRunOn="4853"><Segment range="4611-4808" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="61" name="rpsU3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4611-4808" color="#00e600" type="standard"/></Feature><Feature recentID="62" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4605-4808" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="63" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4818-4850" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4599-4853" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="1,2" nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="3" name="KROL42 OE_rpsU3R" sequence="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4779-4819" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4779-4819" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4779-4819" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4779-4819" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="4" name="KROL41 OE_rpsU3F" sequence="atatGAATTCaggagggataatatgccaagaataatagttgacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4631" boundStrand="0" annealedBases="GAATTCaggagggataatatgccaagaataatagttgacc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="4592-4631" bases="GAATTCaggagggataatatgccaagaataatagttgacc"/></BindingSite><BindingSite simplified="1" location="4592-4631" boundStrand="0" annealedBases="GAATTCaggagggataatatgccaagaataatagttgacc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="4592-4631" bases="GAATTCaggagggataatatgccaagaataatagttgacc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="21:42:47">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  \     n  X   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     .4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61DkA	S)~bjUCd,zSנ75QUW8Q;eYJU6ghVFHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i              W   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" 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name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4851-5208" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4851-4879" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4902-4999" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5096-5190" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4849" maxFusedRunOn="4849"><Segment range="4610-4804" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="60" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4610-4804" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4814-4846" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="62" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="4569-4598" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="4569-4598" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4598" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="4569-4598" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="3" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4775-4815" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="4775-4815" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="4775-4815" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="4775-4815" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="4" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4628" boundStrand="0" annealedBases="GAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4592-4628" bases="GAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="4592-4628" boundStrand="0" annealedBases="GAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4592-4628" bases="GAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:4:46">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Hr        }   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     S4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:AkO6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="multiple cloning site"/></Q></Feature><Feature recentID="3" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="4" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="5" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="6" name="'mini-Tn7T" directionality="2" type="misc_signal" 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mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="17" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="18" name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="24" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="25" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="30" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="31" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="32" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="33" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="34" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="35" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="36" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="37" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="38" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="48" name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="49" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="50" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="51" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="52" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="53" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="54" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="55" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="56" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>97da295a-ac06-4ba6-951b-43fb4ef45dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="4:0:0">2016.10.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
        _          f	ٕ_Mn镝%)f$RyZy嘤YoyhVh$@v\WjS@         -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="1"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-264" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  A<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="0-39" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component hybridizedRange="0-39" bases="atatggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component hybridizedRange="0-39" bases="atatggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="241-273" boundStrand="1" annealedBases="atatgaattcccctctcacctcgcttaaatgcc" meltingTemperature="64"><Component hybridizedRange="241-273" bases="atatgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="241-273" boundStrand="1" annealedBases="atatgaattcccctctcacctcgcttaaatgcc" meltingTemperature="64"><Component hybridizedRange="241-273" bases="atatgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>60404d3f-5659-4a91-8a87-2be68693a367</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:25:13">2019.12.19</Created>
<LastModified UTC="16:25:13">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       u  *        *Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf@2iQQ,FQ1T8I^rgU=zsUUxUQ5dP0fxfadi	YAVfz|}+	U]=TU^wNDhsUhXzdl쑤Nix(Wz}YQSUFTӕ]EG)5;9MY.k45GU	)^Sh7GONtUl:.PU[iAIk[YSPƮ>CS5i1jTR>Jc[TMlNNP Q[]ETD$ЕZ1Te55vǳTM+`Gc&;QitV]M9w5S]GSaJB$EK^zCFUMfNdQTT$l{jem9)[mZn*V滪[+cWϙ:wiZIRNKEb}Zz;jD%d!b^f)\yncQz^fuTOI%V旮UY[ՂNk૪媩eWjhZԛmfؕYvYѹޗuKF:Ƃb[ITꎕ9yxf[bamUf񖆖#Y骕jn(^	[UٶgYmyM㢳RReYꕅY%%I(b X}a^ZT:_JNnnNm~!^n_hC^Qf+9uiMکz-fukWi%]yeURJuTZRRJkUHgUI*U#S5iUUufeDY@UoEy''`jFתEaykX]UmyUQM(rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]V9PSܑSEpabӞ\Q4$`NG©Sh(?RWPn3@QmDtAh]F
:CØxG[;<+ËL+Q#X^4CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H    (  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="14"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7466" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4716-4765" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="810-842" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="971-993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="4" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="5" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5666-6523" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="6" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4854-5645" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="7" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2659-3675" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="8" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2653" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="9" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7840.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="845" maxFusedRunOn="845"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="16:32:46">2019.1.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:52:23">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
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߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <?xml version="1.0"?><HistoryTree><Node name="pKR61 Tn7_rpsU3-V.dna" type="DNA" seqLen="5195" ID="10" circular="1" operation="replace"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4592..4592" colors="red"/></TopStrand><BottomStrand><ColorRange range="4592..4592" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="4592" val2="4609"/><Node name="pKR61 Tn7_rpsU3-V.dna" type="DNA" seqLen="5212" ID="9" circular="1" resurrectable="1" operation="replace"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4855-5212" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4605-4808" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4818-4850" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4592..4609" colors="white"/></TopStrand><BottomStrand><ColorRange range="4592..4609" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4598..4852" colors="red"/></TopStrand><BottomStrand><ColorRange range="4598..4852" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="4598" val2="4848"/><Node name="pKR61 Tn7_rpsU3.dna" type="DNA" seqLen="5208" ID="8" circular="1" resurrectable="1" operation="insertFragments"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4851-5208" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0"><Segment range="4610-4804" color="#ff0000" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKR"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4814-4846" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><RegeneratedSite name="KpnI" pos="4337" siteCount="1"/><RegeneratedSite name="EcoRI" pos="4593" siteCount="1"/><RegeneratedSite name="BamHI" pos="4850" siteCount="1"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4598..4848" colors="white"/></TopStrand><BottomStrand><ColorRange range="4598..4848" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4337..4592" colors="red"/><ColorRange range="4593..4849" colors="blue"/></TopStrand><BottomStrand><ColorRange range="4333..4596" colors="red"/><ColorRange range="4597..4853" colors="blue"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" name1="BamHI" name2="KpnI" val1="4375" val2="4337" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="KpnI" name2="EcoRI" val1="9" val2="265" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="EcoRI" name2="BamHI" val1="589" val2="846" siteCount1="1" siteCount2="1"/><Node name="pMP749_(modified (1).dna" type="DNA" seqLen="4733" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4733" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4337..4374" colors="white"/></TopStrand><BottomStrand><ColorRange range="4333..4378" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node><Node name="pcr_rpsU2_promoter.dna" type="DNA" seqLen="274" ID="2" circular="0" upstreamModification="Unmodified" downstreamModification="Unmodified" resurrectable="1" operation="amplifyFragment"><Features><Feature name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0"><Segment range="11-264" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features><Oligo name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"/><Oligo name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"/><Parameter name="polymerase" val="polymerase that creates blunt ends"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="9..264" colors="red"/><ColorRange range="0..8" colors="white"/><ColorRange range="265..273" colors="white"/></TopStrand><BottomStrand><ColorRange range="5..268" colors="red"/><ColorRange range="0..4" colors="white"/><ColorRange range="269..273" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="0..9" colors="red"/><ColorRange range="264..273" colors="red"/></TopStrand><BottomStrand><ColorRange range="0..9" colors="red"/><ColorRange range="264..273" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="amplify" val1="1002702" val2="1002955"/><Node name="LVSgenomeWithPrimers.dna" type="DNA" seqLen="1895994" ID="1" circular="1" operation="invalid"><Primers><HybridizationParams minContinuousMatchLen="15" allowMismatch="0" minMeltingTemperature="40"/><Primer recentID="0" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1002702-1002731" boundStrand="0" annealedBases="aacctttaaccatataaatttctaaagaat" meltingTemperature="53"><Component bases="atatggtacc"/><Component hybridizedRange="1002702-1002731" bases="aacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="1" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1002933-1002955" boundStrand="1" annealedBases="ccctctcacctcgcttaaatgcc" meltingTemperature="61"><Component bases="atatgaattc"/><Component hybridizedRange="1002933-1002955" bases="ccctctcacctcgcttaaatgcc"/></BindingSite></Primer></Primers><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1002702..1002955" colors="black"/><ColorRange range="1002956..1002701" colors="white"/></TopStrand><BottomStrand><ColorRange range="1002702..1002955" colors="black"/><ColorRange range="1002956..1002701" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node><Node name="pKR7 pF-rpsU2-V.dna" type="DNA" seqLen="7466" ID="7" circular="1" resurrectable="1" operation="replace"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2659-3675" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1136-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5666-6523" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4854-5645" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2234-2653" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4716-4765" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="810-842" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="971-993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="589..845" colors="blue"/><ColorRange range="0..588" colors="white"/><ColorRange range="846..7465" colors="white"/></TopStrand><BottomStrand><ColorRange range="593..849" colors="blue"/><ColorRange range="0..592" colors="white"/><ColorRange range="850..7465" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="600..799" colors="red"/></TopStrand><BottomStrand><ColorRange range="600..799" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="600" val2="800"/><Node name="pKR7 pF-rpsU2-V.dna" type="DNA" seqLen="7467" ID="6" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="600..800" colors="white"/></TopStrand><BottomStrand><ColorRange range="600..800" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="594..599" colors="red"/></TopStrand><BottomStrand><ColorRange range="594..599" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="594"/><Node name="pKR6 pF-rpsU1-V.dna" type="DNA" seqLen="7461" ID="5" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="594..794" colors="red"/></TopStrand><BottomStrand><ColorRange range="594..794" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="594"/><Node name="pKR6 pF-rpsU1-V.dna" type="DNA" seqLen="7260" ID="4" circular="1" resurrectable="1" operation="remove" hideHistory="1"><Features><Feature name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="594..1500" colors="blue-black"/><ColorRange range="6947..593" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="594..1500" colors="blue-black"/><ColorRange range="6947..593" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="594" val2="1298"/><Node name="pKR6 pF-rpsU1-V.dna" type="DNA" seqLen="7965" ID="3" circular="1" resurrectable="1" operation="invalid"><Features><Feature 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text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MRILLAEDDLHLGEGLLEALQKEGLIVNLVSDGEAAQTFIESGLYDIVVLDIGMPIKTGLEVLRNIRNRGIKVPIILLTARDGLEDRIKGLDLGADDYLTKPFELKELVARIKAISRRIDTRSGKSVNEEIRFGDYSFNPSSETVTKDGVIIPVSKKELALLAILVQNAGRVVPKTQLLEEIYSTDKEMDTNTLEVHMHNLRKKINIPNFIQTIRGVGYFVQKDKVIK"/></Q></Feature><Feature name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1299..2293" colors="blue-black"/><ColorRange range="7564..593" colors="black-red"/><ColorRange range="594..1298" colors="white"/></TopStrand><BottomStrand><ColorRange range="1299..2293" colors="blue-black"/><ColorRange range="7564..593" colors="black-red"/><ColorRange range="594..1298" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></Node></Node></Node></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4889-4986" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5083-5177" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4593" color="#00ff00" type="standard"/></Feature><Feature recentID="60" name="30S ribosomal protein S21" directionality="1" translationMW="7816.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4836" maxFusedRunOn="4836"><Segment range="4594-4791" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPRIIVDPKKPFDISLRNFKRACEKAGIKQELRDRQHYVKPTQKRKIAKKAAISKAKKEARRSYSY"/></Q></Feature><Feature recentID="61" name="rpsU3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4594-4791" color="#00e600" type="standard"/></Feature><Feature recentID="62" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4594-4791" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="63" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4801-4833" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4594-4836" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL42 OE_rpsU3R" sequence="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4762-4802" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4762-4802" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="2" name="KROL41 OE_rpsU3F" sequence="atatGAATTCaggagggataatatgccaagaataatagttgacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4587-4614" boundStrand="0" annealedBases="gagggtatgccaagaataatagttgacc" meltingTemperature="52"><Component bases="atatGAATTCag"/><Component hybridizedRange="4587-4591" bases="gaggg"/><Component bases="ataa"/><Component hybridizedRange="4592-4614" bases="tatgccaagaataatagttgacc"/></BindingSite><BindingSite simplified="1" location="4592-4614" boundStrand="0" annealedBases="tatgccaagaataatagttgacc" meltingTemperature="52"><Component bases="atatGAATTCaggagggataa"/><Component hybridizedRange="4592-4614" bases="tatgccaagaataatagttgacc"/></BindingSite></Primer><Primer recentID="3" name="KROL290 rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" dateAdded="2019-12-19T21:43:09Z"><BindingSite location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="4" name="KROL291 VSVG_bamHI_r" sequence="atatggatcCTTATTTACCTAATCTATTCATT" dateAdded="2019-12-19T21:43:09Z"><BindingSite location="4814-4842" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="4814-4842" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite><BindingSite simplified="1" location="4814-4842" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="4814-4842" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite></Primer><Primer recentID="5" name="KROL296 rpsU3_p2_F" sequence="gtgagagggtatgccaagaataatagttgaccctaaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:44:40Z"><BindingSite location="4583-4619" boundStrand="0" annealedBases="gtgagagggtatgccaagaataatagttgaccctaaa" meltingTemperature="63"><Component hybridizedRange="4583-4619" bases="gtgagagggtatgccaagaataatagttgaccctaaa"/></BindingSite><BindingSite simplified="1" location="4583-4619" boundStrand="0" annealedBases="gtgagagggtatgccaagaataatagttgaccctaaa" meltingTemperature="63"><Component hybridizedRange="4583-4619" bases="gtgagagggtatgccaagaataatagttgaccctaaa"/></BindingSite></Primer><Primer recentID="6" name="KROL297 rpsU3_p2_R" sequence="ttcttggcataccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:45:37Z"><BindingSite location="4569-4602" boundStrand="1" annealedBases="ttcttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component hybridizedRange="4569-4602" bases="ttcttggcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4602" boundStrand="1" annealedBases="ttcttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component hybridizedRange="4569-4602" bases="ttcttggcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="7" name="KROL263 pKR38_FR1R" sequence="atatgcggccgcctaataagaataagaccttcttgcttcttttttag" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4759-4802" boundStrand="1" annealedBases="atatgcggccgcataagaataagaccttcttgcttcttttttag" meltingTemperature="62"><Component hybridizedRange="4791-4802" bases="atatgcggccgc"/><Component bases="cta"/><Component hybridizedRange="4759-4790" bases="ataagaataagaccttcttgcttcttttttag"/></BindingSite><BindingSite simplified="1" location="4759-4802" boundStrand="1" annealedBases="atatgcggccgcataagaataagaccttcttgcttcttttttag" meltingTemperature="62"><Component hybridizedRange="4791-4802" bases="atatgcggccgc"/><Component bases="cta"/><Component hybridizedRange="4759-4790" bases="ataagaataagaccttcttgcttcttttttag"/></BindingSite></Primer><Primer recentID="8" name="KROL42 OE_rpsU3Ra" sequence="atatGCGGCCGCataagaataagaccttcttgcttcttttt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4762-4802" boundStrand="1" annealedBases="atatGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="atatGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4762-4802" boundStrand="1" annealedBases="atatGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="atatGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="9" name="KROL214 ChaC-V_FR1_R" sequence="atatCAATTGaattcttatttacctaatctattcatt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4814-4836" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="4814-4836" bases="cttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="4814-4836" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="4814-4836" bases="cttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="11" name="KROL87 Arb1" sequence="GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="92-106" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="47"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="92-106" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="1066-1080" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="36"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="1066-1080" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="1424-1438" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="47"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="1424-1438" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="4619-4633" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="37"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4619-4633" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="4795-4810" boundStrand="0" annealedBases="CNNNNNNNNNNGATAT" meltingTemperature="45"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="4795-4810" bases="CNNNNNNNNNNGATAT"/></BindingSite><BindingSite location="4842-4864" boundStrand="0" annealedBases="ACTAGTNNNNNNNNNNGATAT" meltingTemperature="45"><Component bases="GGCCACGCGTCG"/><Component hybridizedRange="4842-4847" bases="ACTAGT"/><Component bases="AC"/><Component hybridizedRange="4850-4864" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="103-117" boundStrand="1" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="36"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="103-117" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="206-220" boundStrand="1" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="40"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="206-220" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="643-681" boundStrand="1" annealedBases="GCCACGCTCACNNNNNNNNNNGATAT" meltingTemperature="41"><Component bases="G"/><Component hybridizedRange="678-681" bases="GCCA"/><Component hybridizedRange="674-676" bases="CGC"/><Component bases="G"/><Component hybridizedRange="671-672" bases="TC"/><Component bases="G"/><Component hybridizedRange="668-669" bases="AC"/><Component bases="TAGTAC"/><Component hybridizedRange="643-657" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite location="4861-4875" boundStrand="1" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="56"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4861-4875" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="92-106" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="47"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="92-106" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="1066-1080" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="36"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="1066-1080" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="1424-1438" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="47"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="1424-1438" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="4619-4633" boundStrand="0" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="37"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4619-4633" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="4795-4810" boundStrand="0" annealedBases="CNNNNNNNNNNGATAT" meltingTemperature="45"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="4795-4810" bases="CNNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="4842-4864" boundStrand="0" annealedBases="ACTAGTNNNNNNNNNNGATAT" meltingTemperature="45"><Component bases="GGCCACGCGTCG"/><Component hybridizedRange="4842-4847" bases="ACTAGT"/><Component bases="AC"/><Component hybridizedRange="4850-4864" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="103-117" boundStrand="1" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="36"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="103-117" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="206-220" boundStrand="1" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="40"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="206-220" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="643-681" boundStrand="1" annealedBases="GCCACGCTCACNNNNNNNNNNGATAT" meltingTemperature="41"><Component bases="G"/><Component hybridizedRange="678-681" bases="GCCA"/><Component hybridizedRange="674-676" bases="CGC"/><Component bases="G"/><Component hybridizedRange="671-672" bases="TC"/><Component bases="G"/><Component hybridizedRange="668-669" bases="AC"/><Component bases="TAGTAC"/><Component hybridizedRange="643-657" bases="NNNNNNNNNNGATAT"/></BindingSite><BindingSite simplified="1" location="4861-4875" boundStrand="1" annealedBases="NNNNNNNNNNGATAT" meltingTemperature="56"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4861-4875" bases="NNNNNNNNNNGATAT"/></BindingSite></Primer><Primer recentID="12" name="KROL89 Arb6" sequence="GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="1452-1483" boundStrand="0" annealedBases="CACGCGACNNNNNNNNNNACGCC" meltingTemperature="57"><Component bases="GGC"/><Component hybridizedRange="1452-1457" bases="CACGCG"/><Component bases="TCGACTAGT"/><Component hybridizedRange="1467-1483" bases="ACNNNNNNNNNNACGCC"/></BindingSite><BindingSite location="2049-2080" boundStrand="0" annealedBases="ACGCGCGANNNNNNNNNNACGCC" meltingTemperature="50"><Component bases="GGCC"/><Component hybridizedRange="2049-2053" bases="ACGCG"/><Component bases="T"/><Component hybridizedRange="2054-2056" bases="CGA"/><Component bases="CTAGTAC"/><Component hybridizedRange="2066-2080" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite location="2494-2508" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="52"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="2494-2508" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite location="3772-3786" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="59"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="3772-3786" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite location="3867-3882" boundStrand="0" annealedBases="CNNNNNNNNNNACGCC" meltingTemperature="51"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="3867-3882" bases="CNNNNNNNNNNACGCC"/></BindingSite><BindingSite location="5036-5050" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="40"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5036-5050" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite location="5058-5103" boundStrand="0" annealedBases="GCCACTCGNNNNNNNNNNACGCC" meltingTemperature="43"><Component bases="G"/><Component hybridizedRange="5058-5062" bases="GCCAC"/><Component bases="GCG"/><Component hybridizedRange="5066-5068" bases="TCG"/><Component bases="ACTAGTAC"/><Component hybridizedRange="5089-5103" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite location="1751-1765" boundStrand="1" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="51"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="1751-1765" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite location="5009-5023" boundStrand="1" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="52"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5009-5023" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="1452-1483" boundStrand="0" annealedBases="CACGCGACNNNNNNNNNNACGCC" meltingTemperature="57"><Component bases="GGC"/><Component hybridizedRange="1452-1457" bases="CACGCG"/><Component bases="TCGACTAGT"/><Component hybridizedRange="1467-1483" bases="ACNNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="2066-2080" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="50"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="2066-2080" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="2494-2508" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="52"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="2494-2508" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="3772-3786" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="59"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="3772-3786" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="3867-3882" boundStrand="0" annealedBases="CNNNNNNNNNNACGCC" meltingTemperature="51"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="3867-3882" bases="CNNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="5036-5050" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="40"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5036-5050" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="5089-5103" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="43"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5089-5103" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="1751-1765" boundStrand="1" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="51"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="1751-1765" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="5009-5023" boundStrand="1" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="52"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5009-5023" bases="NNNNNNNNNNACGCC"/></BindingSite></Primer><Primer recentID="13" name="KROL120 ?rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4579-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcg" meltingTemperature="62"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="4579-4600" bases="cttggcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="4579-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcg" meltingTemperature="62"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="4579-4600" bases="cttggcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="14" name="KROL293 rpsU1_p2_R" sequence="tacttagcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4569-4600" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="ta"/><Component hybridizedRange="4598-4600" bases="ctt"/><Component bases="a"/><Component hybridizedRange="4569-4596" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4600" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="ta"/><Component hybridizedRange="4598-4600" bases="ctt"/><Component bases="a"/><Component hybridizedRange="4569-4596" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="15" name="KROL295 rpsU2_p2_R" sequence="cgcttggcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4569-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component bases="cg"/><Component hybridizedRange="4569-4600" bases="cttggcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component bases="cg"/><Component hybridizedRange="4569-4600" bases="cttggcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="16" name="KROL164 P035 INT_VSVG" sequence="TTTACCTAATCTATTCATTTCAATATCAG" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4804-4832" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="4804-4832" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite><BindingSite simplified="1" location="4804-4832" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="4804-4832" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite></Primer><Primer recentID="17" name="KROL240 katg-ck-1" sequence="gattacgaattcttatttacctaatctat" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4819-4836" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="4819-4836" bases="cttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="4819-4836" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="4819-4836" bases="cttatttacctaatctat"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:47:9">2020.1.7</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                            d      HO  d             E             Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
