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&=
]  'J p  L)N#	gy 0-			?@67 Ft_
}

U^D  4V>	
9V~g7 BgU	p	

mOR
 b Ez=M!

_Fp}#  i	 	l e   c.	[ S l  7		
-Q1 2 $\?Z	-
9
L L v  ,2WQ		
|/\"   C    wVqke / _      Su)-	6
Jd  & {  z5e`		
jv  xE}in	
+Lx  A Q V   *o/' X  ~lC					

|1IFg : [ T;v*(1)* s20		

vx> G ZQf		
A5   52
^vz  2+k ;U  =lUBW1  &!Ep Y
3N o /K(&,			x\  E3D<=	T	
^
T^l]U| d   bY		& 
 % u z   1Vjy4,			
i{c  9 @ P Z  }nuw			H0.)  1Pe		 


wy:T! %. D%k~<	S		
S]kw{[( 	 8 t    0|xv3+	X								-H 7 r s  {2}Yrss	t		

H~PQR\ij<=>?YZ   iv+EZtGu		

I:@># jwF:z?@ :C`au^}C	v			
J3_$  &N,[Hz	
H0A 	T
;Kc
 !Q5i9 k+	U xG/p*3{e 	>L';N
B9c@ A 8N_PoD			
AKCh ;V=&av	
4&H .b	Q` DxZC~w	
KTpaht% '-I	


BMH,  ] 9,O		+
}3 > b g~:{)	u.18+ < J\

.IK e	0<  Gi		 "  !2
 /B^(

d94 ?U
L#M 5	 2'<>js: *q"zXq/ hR

6qf ' n!
W *  
va5'D H[g]	<
o
XfqV
 ;/,[	
K
C,NV\ Hl2	
29	 @q>94|  uA0	
4
&+H AyB[U cH,OSf ?		

]_FQSB U(	F
a



=`a@ 
M4<	
{dh  #		O
v


xC$:"A7Gl 9AQ5	
D iy R#S`*hE	BuLk_ I d (k			[

e&  mO8pz	c

	nci W[I6	!+I9 <K\bo}9wc	.b'X\Bp 'HW9	
O;  }O>_s	
X

51UXH 	4;  X^	}		

?)t 5    #/c	8Za	 @	
Rs	 [	!	A7
 y{}x		
i  pD@	
Cu
& Ev	H



LUqb>eD  P4i $JNe	$
0
}m%-! o(.m		g
y



fwnI "	
%fI6 L
	8CNQ	"6  #[-6	,
~  ^     RPd :D		{EfKw	 r4u4j  D2<;T	2TM $ ? c ai	
Gvx9(   huC1qF*	W	r			O;, 9`y|B	S
b/? KP	o4d 7M%OB

oJ`sG = }(	*


|GJ	  ]>
/"L 1&=W ) G	f.
J

W	[ b
+N=% 		E




<gFP c"4j	

tdm%  |HGW

-:o  "?]		(   		@	dC !#df
x

eH$55  "3Czv  #  t0_xR

FNa:> |OA	'	)
n!rFI 	D



$O b		V
s9n ~ @&	Q

EM  	%	
N  TTTN  TTNT  TNTA  NTAG  _TCAN  `CANG  aANGC  bNGCC  kTGAN  lGANT  mANTC  nNTCA  CGAN  GANG  ANGC  NGCA  )  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                  	                                                                                       	            
   	      	               	                                                                                                              
                                                      K   J         :   @            &   >                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                        	                                 	    
 	  	     	                                      
                  K J   : @    & >   a0  a  a  a  dD  g  l		
K?".?)
+P>:b3	08_e
4 E
>Q8ZG
e
n
u

G84# :# :# :8>3:LN1j,0@ n;_
^G\t
nGYX ]sB	t
" 
}
}bZ
} 
ot
	bIt
5j	t
nrG2MG;,K
sOVN;,K
6f0H,bA 
}}M	=
%1 CZ/
C

S4y :s67	+6(o	 B	NU
y y  B	
}8,Z~p1HA A
nc2GM j+,5
\ Z	,67+7 :`u34#d
$I-blb
7P!bh`&
B
k1&= SDKTpa&
lb
bh7P!?g ZT|ml& 
b

7V;P!bh4#d
1&=$	
rTr{

D` 3h	lcj	d%P{O@	7MO
Jo(f H ag
Z
*Mc a7o(MPO
Jf& u

"R	D


O D#$\l@E]2	29S'e-_!D	
AKH_xR
a>=4rO


'g :SM	=
%1|~{.D@#E'g: $\]& 

V;u
& `K	"Qa_py<?		

]_FQSB		E




<gFP&) 
~

Vpep.  1
]N_KPY:	.
#6T`
u

^_E`6Rk #[-6	,
~7<8-		L
a$,7`B
lY3<1&=W	4; N_z`rKPY:koL	[	"
.
B
d


#6l&ltY3<  A Q V    *	oX4xE}i^	n	}		


;+?)L/xt ?tZ 4V>	
9V~g7c"!4#dj	
f
x


1et&dHm%=W$55 P94Ai Q5$JNe		
$
0
1}&mD=W %iy-   Ts^vt*:	|OA	'	)
n!rFI   *@
D(E
 % u z   1Vjy4,			
i{c   ?N_t+KPY:<
w		
.
#6EZmEFk+	U  `f     q  H   	     '_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%e6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZS_/eiweWH"攱twngj     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>                  G   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  J<?xml version="1.0"?><Features nextValidID="66"><Feature recentID="0" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="465-497" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="cold shock protein" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="69" maxRunOn="267" maxFusedRunOn="267"><Segment range="261-261" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="261-261" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="500" maxFusedRunOn="500"><Segment range="261-455" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="261-455" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="437" maxFusedRunOn="437"><Segment range="455-455" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="455-455" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="747-841" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="8" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="553-650" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="9" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5180-530" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="10" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5147-5173" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="11" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5043-5072" color="#a020f0" type="standard"/></Feature><Feature recentID="12" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5006-5171" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="13" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5006-859" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4234-4234" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4233-4233" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4231-4231" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4230-4230" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4229-4229" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4228-4228" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4227-4227" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4225-4225" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4224-4224" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4214-4235" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="24" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4125-4792" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="25" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3977" maxFusedRunOn="3977"><Segment range="3732-3974" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3646-3646" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3645-3645" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="28" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3643-3643" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3641-3641" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3639-3639" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3638-3638" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3637-3637" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3636-3636" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3635-3635" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3625-3646" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="36" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3977" maxFusedRunOn="3977"><Segment range="3117-3731" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2841-2860" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="38" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2337-2535" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="39" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2326-2535" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="40" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2318-2321" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="41" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2318-2321" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="42" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2289-2313" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="43" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2250-2283" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="44" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2200-2227" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="45" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2034-2077" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1997-1997" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1996-1996" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1995-1995" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1993-1993" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1991-1991" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="51" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1989-1989" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1986-1986" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="53" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1984-1984" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1962-1997" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1168-1983" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="56" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1078-1078" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="57" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1070-1089" color="#a020f0" type="standard"/></Feature><Feature recentID="58" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1067-1067" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="59" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1043-1043" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="60" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1043-1078" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="61" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="957-981" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="62" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-951" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="63" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-894" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="64" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1042-1112" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="65" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-259" color="#00ff00" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290_rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="5191-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5191-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="5191-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5191-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="236-258" boundStrand="1" annealedBases="ccctctcacctcgcttaaatgcc" meltingTemperature="61"><Component bases="atatgaattc"/><Component hybridizedRange="236-258" bases="ccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="236-258" boundStrand="1" annealedBases="ccctctcacctcgcttaaatgcc" meltingTemperature="61"><Component bases="atatgaattc"/><Component hybridizedRange="236-258" bases="ccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="3" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="427-466" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="427-466" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="427-466" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="427-466" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="4" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="260-281" boundStrand="0" annealedBases="atgctaagtattagagtagatg" meltingTemperature="50"><Component bases="atatGAATTCaggaggactata"/><Component hybridizedRange="260-281" bases="atgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="260-281" boundStrand="0" annealedBases="atgctaagtattagagtagatg" meltingTemperature="50"><Component bases="atatGAATTCaggaggactata"/><Component hybridizedRange="260-281" bases="atgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>7ab4100a-3115-4fed-ad7c-e0b173353f97</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:32:24">2019.12.19</Created>
<LastModified UTC="21:14:35">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  `   
  n  Y   	     '_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LB ZAX&$a7Wچe*Q^r:1U8&N7=tUQQUSUQjDښ0fxfadi	YA퍆l5;LFUC>jSʔUi.NYUs_+սBx]NӖfZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>              X   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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terminator"/></Q></Feature><Feature recentID="9" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5197-547" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="10" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5164-5190" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="11" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5060-5089" color="#a020f0" type="standard"/></Feature><Feature recentID="12" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5023-5188" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="13" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5023-876" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4251-4251" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4250-4250" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4248-4248" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4247-4247" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4246-4246" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4245-4245" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4244-4244" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4242-4242" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4241-4241" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4231-4252" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="24" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4142-4809" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="25" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3994" maxFusedRunOn="3994"><Segment range="3749-3991" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3663-3663" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3662-3662" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="28" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3660-3660" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3658-3658" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3656-3656" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3655-3655" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3654-3654" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3653-3653" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3652-3652" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3642-3663" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="36" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3994" maxFusedRunOn="3994"><Segment range="3134-3748" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2858-2877" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="38" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2354-2552" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="39" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2343-2552" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="40" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2335-2338" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="41" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2335-2338" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="42" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2306-2330" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="43" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2267-2300" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="44" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2217-2244" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="45" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2051-2094" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2014-2014" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2013-2013" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2012-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2010-2010" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2008-2008" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="51" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2006-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2003-2003" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="53" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2001-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1979-2014" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1185-2000" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="56" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1095-1095" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="57" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1087-1106" color="#a020f0" type="standard"/></Feature><Feature recentID="58" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1084-1084" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="59" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1060-1060" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="60" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1060-1095" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="61" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="974-998" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="62" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="935-968" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="63" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="885-911" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="64" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1059-1129" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="65" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-259" color="#00ff00" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290_rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="5208-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5208-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="5208-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5208-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="236-265" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="236-265" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="236-265" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="236-265" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="3" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="444-483" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="444-483" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="444-483" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="444-483" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="4" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="259-298" boundStrand="0" annealedBases="GAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="259-298" bases="GAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="259-298" boundStrand="0" annealedBases="GAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="259-298" bases="GAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>7ab4100a-3115-4fed-ad7c-e0b173353f97</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:32:24">2019.12.19</Created>
<LastModified UTC="21:14:27">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  _   	  n  Z   	     '_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S95YVwvUt)b.iK)Gxfv
     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>              Y   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="65"><Feature recentID="0" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="483-515" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="cold shock protein" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="273-279" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="273-279" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="518" maxFusedRunOn="518"><Segment range="279-473" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="279-473" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="455" maxFusedRunOn="455"><Segment range="473-473" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="473-473" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-859" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="8" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="571-668" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="9" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5198-548" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="10" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5165-5191" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="11" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5061-5090" color="#a020f0" type="standard"/></Feature><Feature recentID="12" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5024-5189" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="13" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5024-877" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4252-4252" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4251-4251" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4249-4249" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4248-4248" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4247-4247" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4246-4246" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4245-4245" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4243-4243" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4242-4242" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4232-4253" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="24" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4143-4810" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="25" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3995" maxFusedRunOn="3995"><Segment range="3750-3992" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3664-3664" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3663-3663" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="28" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3661-3661" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3659-3659" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3657-3657" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3656-3656" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3655-3655" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3654-3654" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3653-3653" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3643-3664" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="36" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3995" maxFusedRunOn="3995"><Segment range="3135-3749" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2859-2878" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="38" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2355-2553" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="39" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2344-2553" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="40" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2336-2339" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="41" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2336-2339" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="42" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2307-2331" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="43" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2268-2301" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="44" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2218-2245" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="45" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2052-2095" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2015-2015" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2014-2014" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2013-2013" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2009-2009" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="51" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2007" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2004-2004" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="53" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2002-2002" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1980-2015" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1186-2001" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="56" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1096-1096" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="57" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1088-1107" color="#a020f0" type="standard"/></Feature><Feature recentID="58" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1085-1085" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="59" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1061-1061" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="60" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1061-1096" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="61" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="975-999" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="62" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="936-969" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="63" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="886-912" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="64" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1060-1130" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290_rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="5209-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5209-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="5209-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5209-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="rpsU2_promoter_primer_2" sequence="atatgaattcaccctctcacctcgcttaaatgccagtttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="229-266" boundStrand="1" annealedBases="tgaattcaccctctcacctcgcttaaatgccagtttat" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="229-266" bases="tgaattcaccctctcacctcgcttaaatgccagtttat"/></BindingSite><BindingSite simplified="1" location="229-266" boundStrand="1" annealedBases="tgaattcaccctctcacctcgcttaaatgccagtttat" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="229-266" bases="tgaattcaccctctcacctcgcttaaatgccagtttat"/></BindingSite></Primer><Primer recentID="3" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="445-484" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="445-484" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="445-484" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="445-484" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="4" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="259-299" boundStrand="0" annealedBases="tGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component bases="ata"/><Component hybridizedRange="259-299" bases="tGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="259-299" boundStrand="0" annealedBases="tGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component bases="ata"/><Component hybridizedRange="259-299" bases="tGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>7ab4100a-3115-4fed-ad7c-e0b173353f97</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:32:24">2019.12.19</Created>
<LastModified UTC="16:18:14">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        _          f	ٕ_Mn镝%)f$RyZy嘤YoyhVh$@v\WjS@&         -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="1"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-265" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  |<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="rpsU2_promoter_primer" sequence="atatggtaccaacctttaaccatataaatttctaaagaatcctta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="0-44" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaatcctta" meltingTemperature="60"><Component hybridizedRange="0-44" bases="atatggtaccaacctttaaccatataaatttctaaagaatcctta"/></BindingSite><BindingSite simplified="1" location="0-44" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaatcctta" meltingTemperature="60"><Component hybridizedRange="0-44" bases="atatggtaccaacctttaaccatataaatttctaaagaatcctta"/></BindingSite></Primer><Primer recentID="2" name="rpsU2_promoter_primer_2" sequence="atatgaattcaccctctcacctcgcttaaatgccagtttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T15:28:50Z"><BindingSite location="234-274" boundStrand="1" annealedBases="atatgaattcaccctctcacctcgcttaaatgccagtttat" meltingTemperature="66"><Component hybridizedRange="234-274" bases="atatgaattcaccctctcacctcgcttaaatgccagtttat"/></BindingSite><BindingSite simplified="1" location="234-274" boundStrand="1" annealedBases="atatgaattcaccctctcacctcgcttaaatgccagtttat" meltingTemperature="66"><Component hybridizedRange="234-274" bases="atatgaattcaccctctcacctcgcttaaatgccagtttat"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>2c917b51-3a79-485d-b42b-9c54a565b575</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:28:57">2019.12.19</Created>
<LastModified UTC="15:28:57">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  &g     u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  N<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="8" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="9" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="10" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="11" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="12" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="13" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="14" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="15" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="17" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL44 Seq_pF_1R" sequence="TAGCTCTGTTGAGCTGTGTTGTAAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="971-995" boundStrand="1" annealedBases="TAGCTCTGTTGAGCTGTGTTGTAAG" meltingTemperature="59"><Component hybridizedRange="971-995" bases="TAGCTCTGTTGAGCTGTGTTGTAAG"/></BindingSite><BindingSite simplified="1" location="971-995" boundStrand="1" annealedBases="TAGCTCTGTTGAGCTGTGTTGTAAG" meltingTemperature="59"><Component hybridizedRange="971-995" bases="TAGCTCTGTTGAGCTGTGTTGTAAG"/></BindingSite></Primer><Primer recentID="1" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="773-812" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="773-812" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="2" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="584-627" boundStrand="0" annealedBases="atatGAATTCaggaggactataatgctaagtattagagtagatg" meltingTemperature="61"><Component hybridizedRange="584-627" bases="atatGAATTCaggaggactataatgctaagtattagagtagatg"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="18:54:2">2019.2.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
ܒ?G
?r/rHWuꆦPƬ>h)Ua8j]UsuZ3P8ALrEOK8NxnPsS5DTГH9
ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
ZU{I_mXae+mw^ch&%jNZ.㟧^j%q*
uVohin8%^Vf%ꐕ[imlvuי:+>ZE%&[XU^.:T蒉"եM@Y*+=hHZ:)$Fѐg뺥5iJCQ&9ڝyngVzUמUU$\gUI*U!U$VTuTZUR:>YU[YQfTEUVQz[WRzjRvi_)izV%V/h
UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
         	   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:Akmv(h=Ypz(9[d^d)N8iکd^m蕫kOy葚]I\&y_(nZ6N:ҋ>ZTfYjIHItꩨdjIzi+xiNNVDS],JxHMْ(yGd$lkX4hbQyEٵ:Ky$ojV!x:MfUAJff1NjAYꤩ:+MyZOz*binۺ&~J:fvj:}u_9jfꅍIۋijItkju[m2W(+Q1.ڨz*UZUzYRjVjښjYYeWiAifa6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ         -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  m<?xml version="1.0"?><Features nextValidID="99"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4368-6043" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="RPp7" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4368-4377" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="2" name="RPp4" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5817-5843" color="#0080ff" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="3" name="PRP12" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5975-6004" color="#8080ff" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="4" name="200 bp max AT content (91%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5844-6043" color="#0080ff" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="5" name="150 bp max AT content (93.3%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5844-5993" color="#0080ff" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature recentID="6" name="100 bp max AT content (95%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5865-5964" color="#0080ff" type="standard"/><Q name="note"><V text="100 bp max AT content (95%)"/></Q></Feature><Feature recentID="7" name="Potential TSS" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5875-5875" color="#ff8080" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="8" name="Potential -10 element" directionality="2" type="-10_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5882-5887" color="#00ff00" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="9" name="Potential -35 element" directionality="2" type="-35_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5904-5909" color="#00ff00" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="10" name="P431 P_FTL0702_F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5975-6004" color="#50ff50" type="standard"/><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="11" name="P434 FTL0702cmp_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5843-5876" color="#50ff50" type="standard"/><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="12" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4368-5825" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="hypothetical protein" directionality="2" translationMW="53302.96" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4368-5825" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="P364 FTL0702cmp_F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5799-5825" color="#8080ff" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="15" name="P365 FTL0702cmp_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4371-4398" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="16" name="P367 FTL0702seqF1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5494-5518" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="17" name="P368 FTL0702seqR1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4650-4678" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="18" name="P411 FTL0702sp_2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4723-4747" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="19" name="P429 FTL0702_VSVG_2R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5395-5421" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="20" name="P428 FTL0702_VSVG_2F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4371-4398" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="21" name="P433 FTL0702cmp_R2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4368-4395" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="22" name="P435 RT_FTL0702-1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5214-5233" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="23" name="P436 RT_FTL0702-1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5114-5135" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="24" name="P437 RT_FTL0702-2F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4578-4600" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="25" name="P438 RT_FTL0702-2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4474-4495" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="26" name="P439 RT_FTL0702-3F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4995-5016" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="27" name="P440 RT_FTL0702-3R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4931-4950" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="28" name="P444 pKL75_F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4884-4910" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="29" name="P692 PriM_mpk_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5172-5198" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="30" name="P693 PriM_mpk_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5119-5147" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="31" name="P698 Ck_PriM_mpk1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4735-4757" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="32" name="P697 Ck_PriM_mpk1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5210-5233" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="33" name="P700 PriM_mtip1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5466-5493" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="34" name="P701 PriM_mtip1_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5416-5441" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="35" name="P702 PriM_mtip1_R2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4431-4455" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="36" name="P703 CkPriM_mtip1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5732-5761" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="37" name="P704 CkPriM_mtip1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5262-5288" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="38" name="P706 PriM_mtip2_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5466-5493" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="39" name="P705 PriM_mtip2_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5400-5426" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="40" name="P707 PriM_unst1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5179-5205" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="41" name="P708 PriM_unst1_F1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5144-5168" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="42" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="43" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="44" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="45" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="46" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="47" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="48" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="49" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="50" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="51" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="53" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="54" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="55" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="56" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="57" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="58" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="59" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="60" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="61" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="62" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="63" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="64" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="65" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="66" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="67" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="68" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="69" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="70" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="71" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="72" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="73" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="74" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="75" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="76" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="77" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="78" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="79" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="80" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="81" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="82" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="83" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="84" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="85" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="86" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="87" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="88" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="89" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="90" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="91" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="92" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-6409" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="93" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="94" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="95" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="96" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-6080" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="97" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6103-6200" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="98" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6297-6391" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers nextValidID="52"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P820 PriM_mtip1_F3" sequence="atatGGATCCtaaaacaaataataatttttttaaaacaagtc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5837-5868" boundStrand="1" annealedBases="taaaacaaataataatttttttaaaacaagtc" meltingTemperature="50"><Component bases="atatGGATCC"/><Component hybridizedRange="5837-5868" bases="taaaacaaataataatttttttaaaacaagtc"/></BindingSite><BindingSite simplified="1" location="5837-5868" boundStrand="1" annealedBases="taaaacaaataataatttttttaaaacaagtc" meltingTemperature="50"><Component bases="atatGGATCC"/><Component hybridizedRange="5837-5868" bases="taaaacaaataataatttttttaaaacaagtc"/></BindingSite></Primer><Primer recentID="1" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4875-4901" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="4875-4901" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="4875-4901" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="4875-4901" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="2" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5718-5747" boundStrand="1" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="5718-5747" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="5718-5747" boundStrand="1" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="5718-5747" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer><Primer recentID="3" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4416-4442" boundStrand="0" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4416-4442" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="4416-4442" boundStrand="0" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4416-4442" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="4" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5158-5204" boundStrand="0" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="5158-5167" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="5171-5176" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="5178-5204" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="5158-5204" boundStrand="0" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="5158-5167" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="5171-5176" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="5178-5204" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="5" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5143-5187" boundStrand="1" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="57"><Component hybridizedRange="5178-5187" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="5171-5176" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="5143-5167" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="5143-5187" boundStrand="1" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="57"><Component hybridizedRange="5178-5187" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="5171-5176" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="5143-5167" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="6" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="5465-5492" boundStrand="0" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="5465-5492" boundStrand="0" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="7" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="5399-5426" boundStrand="1" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="55"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="5399-5426" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="5399-5426" boundStrand="1" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="55"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="5399-5426" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="8" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="9" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="5442-5492" boundStrand="0" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="5442-5446" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="5447-5458" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="5447-5492" boundStrand="0" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="5447-5458" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="10" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="5118-5167" boundStrand="1" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="5162-5167" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="5150-5159" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="5118-5146" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="5118-5167" boundStrand="1" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="5162-5167" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="5150-5159" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="5118-5146" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="11" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="5415-5458" boundStrand="1" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="5447-5458" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="5443-5446" bases="AAGA"/><Component hybridizedRange="5415-5441" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="5415-5458" boundStrand="1" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="5447-5458" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="5415-5442" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="12" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="5150-5197" boundStrand="0" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="5150-5159" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="5162-5167" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="5171-5197" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="5150-5197" boundStrand="0" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="5150-5159" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="5162-5167" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="5171-5197" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="13" name="P434 FTL0702cmp_F2" sequence="atatCCCGGGtaaattttaaaacaaataataatttttttaaaac" description="Contains XmaI site"><BindingSite location="5842-5875" boundStrand="1" annealedBases="taaattttaaaacaaataataatttttttaaaac" meltingTemperature="48"><Component bases="atatCCCGGG"/><Component hybridizedRange="5842-5875" bases="taaattttaaaacaaataataatttttttaaaac"/></BindingSite><BindingSite simplified="1" location="5842-5875" boundStrand="1" annealedBases="taaattttaaaacaaataataatttttttaaaac" meltingTemperature="48"><Component bases="atatCCCGGG"/><Component hybridizedRange="5842-5875" bases="taaattttaaaacaaataataatttttttaaaac"/></BindingSite></Primer><Primer recentID="14" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="15" name="P431 compPFTL0702_F" sequence="atatCCCGGGcttttatttctaatgttatcaaacaagttt" description="Contains XmaI site"><BindingSite location="5974-6003" boundStrand="1" annealedBases="cttttatttctaatgttatcaaacaagttt" meltingTemperature="53"><Component bases="atatCCCGGG"/><Component hybridizedRange="5974-6003" bases="cttttatttctaatgttatcaaacaagttt"/></BindingSite><BindingSite simplified="1" location="5974-6003" boundStrand="1" annealedBases="cttttatttctaatgttatcaaacaagttt" meltingTemperature="53"><Component bases="atatCCCGGG"/><Component hybridizedRange="5974-6003" bases="cttttatttctaatgttatcaaacaagttt"/></BindingSite></Primer><Primer recentID="16" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="4367-4394" boundStrand="0" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="4367-4394" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="4367-4394" boundStrand="0" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="4367-4394" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="17" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="4363-4397" boundStrand="0" annealedBases="tGATCttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="ata"/><Component hybridizedRange="4363-4364" bases="tG"/><Component bases="G"/><Component hybridizedRange="4366-4368" bases="ATC"/><Component bases="C"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="4363-4397" boundStrand="0" annealedBases="tGATCttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="ata"/><Component hybridizedRange="4363-4364" bases="tG"/><Component bases="G"/><Component hybridizedRange="4366-4368" bases="ATC"/><Component bases="C"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="18" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="4883-4909" boundStrand="1" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4883-4909" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="4883-4909" boundStrand="1" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4883-4909" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="19" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="5008-5044" boundStrand="0" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="5008-5014" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="5018-5044" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="5008-5044" boundStrand="0" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="5008-5014" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="5018-5044" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="20" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="5394-5420" boundStrand="1" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="5394-5420" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="5394-5420" boundStrand="1" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="5394-5420" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="21" name="P364 FTL0702cmp_F" sequence="atatCCCGGGatgaaaaagacactaactatcgctctt" description="Contains XmaI site"><BindingSite location="5798-5824" boundStrand="1" annealedBases="atgaaaaagacactaactatcgctctt" meltingTemperature="58"><Component bases="atatCCCGGG"/><Component hybridizedRange="5798-5824" bases="atgaaaaagacactaactatcgctctt"/></BindingSite><BindingSite simplified="1" location="5798-5824" boundStrand="1" annealedBases="atgaaaaagacactaactatcgctctt" meltingTemperature="58"><Component bases="atatCCCGGG"/><Component hybridizedRange="5798-5824" bases="atgaaaaagacactaactatcgctctt"/></BindingSite></Primer><Primer recentID="22" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="4989-5014" boundStrand="1" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="57"><Component bases="ttcactaGCA"/><Component hybridizedRange="4989-5014" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="4989-5014" boundStrand="1" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="57"><Component bases="ttcactaGCA"/><Component hybridizedRange="4989-5014" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="23" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="4430-4454" boundStrand="0" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="59"><Component bases="atatGGTACC"/><Component hybridizedRange="4430-4454" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="4430-4454" boundStrand="0" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="59"><Component bases="atatGGTACC"/><Component hybridizedRange="4430-4454" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="24" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="4722-4746" boundStrand="0" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="62"><Component bases="atatGGTACC"/><Component hybridizedRange="4722-4746" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="4722-4746" boundStrand="0" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="62"><Component bases="atatGGTACC"/><Component hybridizedRange="4722-4746" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="25" name="P410 FTL0702sp30_2F" sequence="taaatTaatctgttactaaatattctaataatg" description="0"><BindingSite location="5710-5736" boundStrand="1" annealedBases="aatctgttactaaatattctaataatg" meltingTemperature="49"><Component bases="taaatT"/><Component hybridizedRange="5710-5736" bases="aatctgttactaaatattctaataatg"/></BindingSite><BindingSite simplified="1" location="5710-5736" boundStrand="1" annealedBases="aatctgttactaaatattctaataatg" meltingTemperature="49"><Component bases="taaatT"/><Component hybridizedRange="5710-5736" bases="aatctgttactaaatattctaataatg"/></BindingSite></Primer><Primer recentID="26" name="P409 FTL0702sp30_1R" sequence="acagattAatttacaatttttgcatttaaatc" description="0"><BindingSite location="5730-5761" boundStrand="0" annealedBases="acagattatttacaatttttgcatttaaatc" meltingTemperature="51"><Component hybridizedRange="5730-5736" bases="acagatt"/><Component bases="A"/><Component hybridizedRange="5738-5761" bases="atttacaatttttgcatttaaatc"/></BindingSite><BindingSite simplified="1" location="5730-5761" boundStrand="0" annealedBases="acagattatttacaatttttgcatttaaatc" meltingTemperature="51"><Component hybridizedRange="5730-5736" bases="acagatt"/><Component bases="A"/><Component hybridizedRange="5738-5761" bases="atttacaatttttgcatttaaatc"/></BindingSite></Primer><Primer recentID="27" name="P703 CkPriM_mtip1F" sequence="atttaaatgcaaaaattgtaaatgaatctg" description="0"><BindingSite location="5731-5760" boundStrand="1" annealedBases="atttaaatgcaaaaattgtaaatgaatctg" meltingTemperature="54"><Component hybridizedRange="5731-5760" bases="atttaaatgcaaaaattgtaaatgaatctg"/></BindingSite><BindingSite simplified="1" location="5731-5760" boundStrand="1" annealedBases="atttaaatgcaaaaattgtaaatgaatctg" meltingTemperature="54"><Component hybridizedRange="5731-5760" bases="atttaaatgcaaaaattgtaaatgaatctg"/></BindingSite></Primer><Primer recentID="28" name="P415 FTL0702sp17_2F" sequence="caacttAagtttacgctgatgatttaaatg" description="0"><BindingSite location="5752-5781" boundStrand="1" annealedBases="caacttagtttacgctgatgatttaaatg" meltingTemperature="53"><Component hybridizedRange="5776-5781" bases="caactt"/><Component bases="A"/><Component hybridizedRange="5752-5774" bases="agtttacgctgatgatttaaatg"/></BindingSite><BindingSite simplified="1" location="5752-5781" boundStrand="1" annealedBases="caacttagtttacgctgatgatttaaatg" meltingTemperature="53"><Component hybridizedRange="5776-5781" bases="caactt"/><Component bases="A"/><Component hybridizedRange="5752-5774" bases="agtttacgctgatgatttaaatg"/></BindingSite></Primer><Primer recentID="29" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="4649-4677" boundStrand="0" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="4649-4677" boundStrand="0" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="30" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="4649-4677" boundStrand="1" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="4649-4677" boundStrand="1" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="31" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="5261-5287" boundStrand="0" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="5261-5287" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="5261-5287" boundStrand="0" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="5261-5287" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="32" name="P414 FTL0702sp17_1R" sequence="gtaaactTaagttgttgcgatagttcc" description="0"><BindingSite location="5768-5794" boundStrand="0" annealedBases="gtaaactaagttgttgcgatagttcc" meltingTemperature="52"><Component hybridizedRange="5768-5774" bases="gtaaact"/><Component bases="T"/><Component hybridizedRange="5776-5794" bases="aagttgttgcgatagttcc"/></BindingSite><BindingSite simplified="1" location="5768-5794" boundStrand="0" annealedBases="gtaaactaagttgttgcgatagttcc" meltingTemperature="52"><Component hybridizedRange="5768-5774" bases="gtaaact"/><Component bases="T"/><Component hybridizedRange="5776-5794" bases="aagttgttgcgatagttcc"/></BindingSite></Primer><Primer recentID="33" name="P412 CkFTL0702stp30F" sequence="tcaaggagacaaaaatgaaaaagacac" description="0"><BindingSite location="5812-5838" boundStrand="1" annealedBases="tcaaggagacaaaaatgaaaaagacac" meltingTemperature="57"><Component hybridizedRange="5812-5838" bases="tcaaggagacaaaaatgaaaaagacac"/></BindingSite><BindingSite simplified="1" location="5812-5838" boundStrand="1" annealedBases="tcaaggagacaaaaatgaaaaagacac" meltingTemperature="57"><Component hybridizedRange="5812-5838" bases="tcaaggagacaaaaatgaaaaagacac"/></BindingSite></Primer><Primer recentID="34" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="5493-5517" boundStrand="1" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="5493-5517" boundStrand="1" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="35" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="5493-5517" boundStrand="0" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="5493-5517" boundStrand="0" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="36" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="5209-5232" boundStrand="1" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="5209-5232" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="5209-5232" boundStrand="1" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="5209-5232" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="37" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="4577-4599" boundStrand="1" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="4577-4599" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="4577-4599" boundStrand="1" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="4577-4599" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="38" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="4734-4756" boundStrand="0" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="64"><Component hybridizedRange="4734-4756" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="4734-4756" boundStrand="0" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="64"><Component hybridizedRange="4734-4756" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="39" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="4473-4494" boundStrand="0" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="4473-4494" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="4473-4494" boundStrand="0" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="4473-4494" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="40" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="4994-5015" boundStrand="1" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="56"><Component hybridizedRange="4994-5015" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="4994-5015" boundStrand="1" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="56"><Component hybridizedRange="4994-5015" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="41" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="5113-5134" boundStrand="0" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="5113-5134" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="5113-5134" boundStrand="0" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="5113-5134" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="42" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="4930-4949" boundStrand="0" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="4930-4949" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="4930-4949" boundStrand="0" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="4930-4949" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="43" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="5213-5232" boundStrand="1" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="5213-5232" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="5213-5232" boundStrand="1" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="5213-5232" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="44" name="KROL19 PriMnoCFR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5649-5675" boundStrand="1" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="5649-5675" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="5649-5675" boundStrand="1" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="5649-5675" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="45" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4919-4943" boundStrand="0" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="4919-4943" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="4919-4943" boundStrand="0" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="4919-4943" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="46" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4883-4916" boundStrand="1" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component bases="atatGC"/><Component hybridizedRange="4883-4916" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="4883-4916" boundStrand="1" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component bases="atatGC"/><Component hybridizedRange="4883-4916" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="47" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4525-4564" boundStrand="1" annealedBases="aaaaatgcataattttacagcagctaaaaatagtaaca" meltingTemperature="54"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GC"/><Component hybridizedRange="4525-4553" bases="taattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="4525-4564" boundStrand="1" annealedBases="aaaaatgcataattttacagcagctaaaaatagtaaca" meltingTemperature="54"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GC"/><Component hybridizedRange="4525-4553" bases="taattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="48" name="KROL149 Ck_pKR4_F" sequence="acggtgtcgataacttgacctttag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T14:03:34Z"><BindingSite location="4446-4470" boundStrand="0" annealedBases="acggtgtcgataacttgacctttag" meltingTemperature="59"><Component hybridizedRange="4446-4470" bases="acggtgtcgataacttgacctttag"/></BindingSite><BindingSite simplified="1" location="4446-4470" boundStrand="0" annealedBases="acggtgtcgataacttgacctttag" meltingTemperature="59"><Component hybridizedRange="4446-4470" bases="acggtgtcgataacttgacctttag"/></BindingSite></Primer><Primer recentID="49" name="KROL150 Ck_pKR4_R" sequence="gatatcctaaaaggtatcgaaaatggt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T14:09:25Z"><BindingSite location="5351-5377" boundStrand="1" annealedBases="gatatcctaaaaggtatcgaaaatggt" meltingTemperature="55"><Component hybridizedRange="5351-5377" bases="gatatcctaaaaggtatcgaaaatggt"/></BindingSite><BindingSite simplified="1" location="5351-5377" boundStrand="1" annealedBases="gatatcctaaaaggtatcgaaaatggt" meltingTemperature="55"><Component hybridizedRange="5351-5377" bases="gatatcctaaaaggtatcgaaaatggt"/></BindingSite></Primer><Primer recentID="50" name="KROL152 Ck_pKR5_R" sequence="taactacttgactattcagactattaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T14:38:00Z"><BindingSite location="5057-5084" boundStrand="1" annealedBases="taactacttgactattcagactattaag" meltingTemperature="53"><Component hybridizedRange="5057-5084" bases="taactacttgactattcagactattaag"/></BindingSite><BindingSite simplified="1" location="5057-5084" boundStrand="1" annealedBases="taactacttgactattcagactattaag" meltingTemperature="53"><Component hybridizedRange="5057-5084" bases="taactacttgactattcagactattaag"/></BindingSite></Primer><Primer recentID="51" name="KROL35 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T14:52:12Z"><BindingSite location="4525-4564" boundStrand="1" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="4525-4552" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="4525-4564" boundStrand="1" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="4525-4552" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>592d25e9-94f4-4455-bdb6-bd223d45f268</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="16:44:49">2019.10.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
  Ht       }   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     S4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:AkO6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="37" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="36" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="35" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="34" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="33" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="32" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="31" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="30" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="25" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="24" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="16" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="9" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="8" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="7" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="6" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4733" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="5" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="4" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="3" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="2" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4404" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="1" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4427-4524" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="0" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4621-4715" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>97da295a-ac06-4ba6-951b-43fb4ef45dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="16:44:49">2019.10.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
        ƮZTR

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	|UCFJSu}>cYBњJ:mcqϻ     H$  ĭhZg    YZ   <AlignableSequences trimStringency="Medium"><Sequence name="HT06_Rerun_200721" ID="0" isTrace="1" sortOrder="0" trimmedRange="129..1063"/><Sequence name="HT07_Rerun_200721" ID="1" isTrace="1" sortOrder="1" trimmedRange="92..1049"/></AlignableSequences>        BC N sre```pp23*+/*IMbqqQjZjQj^r* ͐=6         BC g핽5>"M1^[Frw2X. Q<cm16y yy ^eNq:3瞀hH/vgY3kOBdg'E<ܻ:m^/Cx룜ͫ?<j^[τe{<Ki^XK!ڔ]6y-kt@FT)^%M!R
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*F'.upeS0*Ikb(8H}"h)bWJ6\FD5%ݠU Úr*58*xk @NADcƅ#uPROj"(Hͽ+1Ĵ1b!bNi2JnzXl-F!عJdQgߣv#_=s)^S:yƆr+o6đ-yUk+xE|{\CgxOpxx?pO|o!7+X}se~u-ە̾r9O[˩s3_{S|LzPO{?ݿ}V:t7e        BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3641-3641" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3639-3639" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3638-3638" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3637-3637" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3636-3636" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3635-3635" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3625-3646" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="36" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3977" maxFusedRunOn="3977"><Segment range="3117-3731" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature recentID="37" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2841-2860" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="38" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2337-2535" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="39" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2326-2535" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="40" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2318-2321" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="41" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2318-2321" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="42" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2289-2313" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="43" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2250-2283" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="44" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2200-2227" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="45" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2034-2077" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1997-1997" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1996-1996" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1995-1995" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1993-1993" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1991-1991" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="51" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1989-1989" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1986-1986" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="53" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1984-1984" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1962-1997" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1168-1983" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="56" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1078-1078" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="57" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1070-1089" color="#a020f0" type="standard"/></Feature><Feature recentID="58" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1067-1067" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="59" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1043-1043" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="60" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1043-1078" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="61" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="957-981" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="62" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-951" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="63" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="868-894" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="64" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1042-1112" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="65" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6-260" color="#00ff00" type="standard"/></Feature></Features>
 {<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="167-195" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="167-195" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL38 OE_rpsU1R" sequence="atatGCGGCCGCaataaatgctcttctctgagaaattctt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="427-466" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="427-466" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite><BindingSite simplified="1" location="427-466" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattctt" meltingTemperature="67"><Component hybridizedRange="427-466" bases="atatGCGGCCGCaataaatgctcttctctgagaaattctt"/></BindingSite></Primer><Primer recentID="2" name="KROL37 OE_rpsU1F" sequence="atatGAATTCaggaggactataatgctaagtattagagtagatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="260-281" boundStrand="0" annealedBases="atgctaagtattagagtagatg" meltingTemperature="50"><Component bases="atatGAATTCaggaggactata"/><Component hybridizedRange="260-281" bases="atgctaagtattagagtagatg"/></BindingSite><BindingSite simplified="1" location="260-281" boundStrand="0" annealedBases="atgctaagtattagagtagatg" meltingTemperature="50"><Component bases="atatGAATTCaggaggactata"/><Component hybridizedRange="260-281" bases="atgctaagtattagagtagatg"/></BindingSite></Primer><Primer recentID="3" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:17:38Z"><BindingSite location="5191-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5191-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="5191-34" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="5191-34" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="4" name="KROL291  VSVG_bamHI_r" sequence="atatggatcCTTATTTACCTAATCTATTCATT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:21:47Z"><BindingSite location="478-506" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="478-506" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite><BindingSite simplified="1" location="478-506" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="478-506" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite></Primer><Primer recentID="5" name="KROL292 rpsU1_p2_F" sequence="gtgagagggtatgctaagtattagagtagatgagcac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:24:26Z"><BindingSite location="250-286" boundStrand="0" annealedBases="gtgagagggtatgctaagtattagagtagatgagcac" meltingTemperature="63"><Component hybridizedRange="250-286" bases="gtgagagggtatgctaagtattagagtagatgagcac"/></BindingSite><BindingSite simplified="1" location="250-286" boundStrand="0" annealedBases="gtgagagggtatgctaagtattagagtagatgagcac" meltingTemperature="63"><Component hybridizedRange="250-286" bases="gtgagagggtatgctaagtattagagtagatgagcac"/></BindingSite></Primer><Primer recentID="6" name="KROL293 rpsU1_p2_R" sequence="tacttagcataccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:26:51Z"><BindingSite location="236-269" boundStrand="1" annealedBases="tacttagcataccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component hybridizedRange="236-269" bases="tacttagcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite location="673-682" boundStrand="1" annealedBases="cttaaagcc" meltingTemperature="22"><Component bases="tacttagcataccctctcacctcg"/><Component hybridizedRange="677-682" bases="cttaaa"/><Component bases="t"/><Component hybridizedRange="673-675" bases="gcc"/></BindingSite><BindingSite simplified="1" location="236-269" boundStrand="1" annealedBases="tacttagcataccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component hybridizedRange="236-269" bases="tacttagcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="673-682" boundStrand="1" annealedBases="cttaaagcc" meltingTemperature="22"><Component bases="tacttagcataccctctcacctcg"/><Component hybridizedRange="677-682" bases="cttaaa"/><Component bases="t"/><Component hybridizedRange="673-675" bases="gcc"/></BindingSite></Primer><Primer recentID="7" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="429-466" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattc" meltingTemperature="61"><Component hybridizedRange="455-466" bases="atatGCGGCCGC"/><Component bases="tta"/><Component hybridizedRange="429-454" bases="aataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="429-466" boundStrand="1" annealedBases="atatGCGGCCGCaataaatgctcttctctgagaaattc" meltingTemperature="61"><Component hybridizedRange="455-466" bases="atatGCGGCCGC"/><Component bases="tta"/><Component hybridizedRange="429-454" bases="aataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="8" name="KROL214 ChaC-V_FR1_R" sequence="atatCAATTGaattcttatttacctaatctattcatt" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="478-500" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="478-500" bases="cttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="478-500" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="478-500" bases="cttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="9" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="1078-1103" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="1078-1103" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="1078-1103" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="1078-1103" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="10" name="KROL120 ?rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="246-267" boundStrand="1" annealedBases="cttgcataccctctcacctcg" meltingTemperature="56"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="265-267" bases="ctt"/><Component bases="g"/><Component hybridizedRange="246-263" bases="gcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="246-267" boundStrand="1" annealedBases="cttgcataccctctcacctcg" meltingTemperature="56"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="265-267" bases="ctt"/><Component bases="g"/><Component hybridizedRange="246-263" bases="gcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="11" name="KROL87 Arb1" sequence="GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="16-31" boundStrand="1" annealedBases="CNNNNNNNNNNATAT" meltingTemperature="27"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="21-31" bases="CNNNNNNNNNN"/><Component bases="G"/><Component hybridizedRange="16-19" bases="ATAT"/></BindingSite><BindingSite location="49-63" boundStrand="1" annealedBases="NNNNNNNNNNGTAT" meltingTemperature="28"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="53-63" bases="NNNNNNNNNNG"/><Component bases="A"/><Component hybridizedRange="49-51" bases="TAT"/></BindingSite><BindingSite location="69-83" boundStrand="0" annealedBases="NNNNNNNNNNGTAT" meltingTemperature="25"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="69-79" bases="NNNNNNNNNNG"/><Component bases="A"/><Component hybridizedRange="81-83" bases="TAT"/></BindingSite><BindingSite location="76-90" boundStrand="0" annealedBases="NNNNNNNNNNATAT" meltingTemperature="20"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="76-85" bases="NNNNNNNNNN"/><Component bases="G"/><Component hybridizedRange="87-90" bases="ATAT"/></BindingSite><BindingSite location="87-101" boundStrand="1" annealedBases="NNNNNNNNNNATAT" meltingTemperature="24"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="92-101" bases="NNNNNNNNNN"/><Component bases="G"/><Component hybridizedRange="87-90" bases="ATAT"/></BindingSite><BindingSite location="139-165" boundStrand="0" annealedBases="ACTAGCNNNNNNNNNNATAT" meltingTemperature="16"><Component bases="GGCCACGCGTCG"/><Component hybridizedRange="139-143" bases="ACTAG"/><Component bases="TA"/><Component hybridizedRange="145-145" bases="C"/><Component hybridizedRange="151-160" bases="NNNNNNNNNN"/><Component bases="G"/><Component hybridizedRange="162-165" bases="ATAT"/></BindingSite><BindingSite location="143-157" boundStrand="0" annealedBases="NNNNNNNNNNATAT" meltingTemperature="35"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="143-152" bases="NNNNNNNNNN"/><Component bases="G"/><Component hybridizedRange="154-157" bases="ATAT"/></BindingSite><BindingSite location="154-168" boundStrand="1" annealedBases="NNNNNNNNNNATAT" meltingTemperature="25"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="159-168" bases="NNNNNNNNNN"/><Component bases="G"/><Component hybridizedRange="154-157" bases="ATAT"/></BindingSite><BindingSite location="162-176" boundStrand="1" 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hybridizedRange="3623-3625" bases="GCC"/></BindingSite><BindingSite simplified="1" location="3643-3657" boundStrand="0" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="37"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="3643-3653" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="3655-3657" bases="GCC"/></BindingSite><BindingSite simplified="1" location="3695-3709" boundStrand="1" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="38"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="3700-3709" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="3695-3698" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="3745-3759" boundStrand="1" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="44"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="3750-3759" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="3745-3748" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="3859-3873" boundStrand="0" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="42"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="3859-3869" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="3871-3873" bases="GCC"/></BindingSite><BindingSite simplified="1" location="4262-4276" boundStrand="1" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="48"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4266-4276" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="4262-4264" bases="GCC"/></BindingSite><BindingSite simplified="1" location="4298-4319" boundStrand="0" annealedBases="CTAGTNNNNNNNNNNAGCC" meltingTemperature="39"><Component bases="GGCCACGCGTCGA"/><Component hybridizedRange="4298-4302" bases="CTAGT"/><Component bases="AC"/><Component hybridizedRange="4305-4315" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="4317-4319" bases="GCC"/></BindingSite><BindingSite simplified="1" location="4333-4347" boundStrand="0" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="45"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4333-4342" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4344-4347" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4394-4408" boundStrand="1" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="44"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4399-4408" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4394-4397" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4447-4461" boundStrand="1" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="53"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4452-4461" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4447-4450" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4538-4552" boundStrand="0" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="36"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4538-4547" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4549-4552" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4573-4588" boundStrand="1" annealedBases="CNNNNNNNNNNCGCC" meltingTemperature="52"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="4578-4588" bases="CNNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4573-4576" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4631-4645" boundStrand="0" annealedBases="NNNNNNNNNNACGCC" meltingTemperature="59"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4631-4645" bases="NNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="4661-4676" boundStrand="0" annealedBases="CNNNNNNNNNNCGCC" meltingTemperature="54"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="4661-4671" bases="CNNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4673-4676" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4719-4733" boundStrand="1" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="45"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4724-4733" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4719-4722" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4726-4741" boundStrand="0" annealedBases="CNNNNNNNNNNACGCC" meltingTemperature="51"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="4726-4741" bases="CNNNNNNNNNNACGCC"/></BindingSite><BindingSite simplified="1" location="4750-4764" boundStrand="1" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="33"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4754-4764" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="4750-4752" bases="GCC"/></BindingSite><BindingSite simplified="1" location="4768-4782" boundStrand="1" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="43"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4772-4782" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="4768-4770" bases="GCC"/></BindingSite><BindingSite simplified="1" location="4779-4793" boundStrand="1" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="37"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4783-4793" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="4779-4781" bases="GCC"/></BindingSite><BindingSite simplified="1" location="4829-4843" boundStrand="0" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="44"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4829-4838" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4840-4843" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4854-4868" boundStrand="0" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="46"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4854-4863" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4865-4868" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4908-4922" boundStrand="0" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="46"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4908-4917" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4919-4922" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4923-4938" boundStrand="0" annealedBases="CNNNNNNNNNNCGCC" meltingTemperature="49"><Component bases="GGCCACGCGTCGACTAGTA"/><Component hybridizedRange="4923-4933" bases="CNNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4935-4938" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4961-4975" boundStrand="0" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="41"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="4961-4970" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="4972-4975" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="4984-5000" boundStrand="0" annealedBases="ACNNNNNNNNNNAGCC" meltingTemperature="42"><Component bases="GGCCACGCGTCGACTAGT"/><Component hybridizedRange="4984-4996" bases="ACNNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="4998-5000" bases="GCC"/></BindingSite><BindingSite simplified="1" location="5009-5023" boundStrand="1" annealedBases="NNNNNNNNNNCGCC" meltingTemperature="35"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5014-5023" bases="NNNNNNNNNN"/><Component bases="A"/><Component hybridizedRange="5009-5012" bases="CGCC"/></BindingSite><BindingSite simplified="1" location="5169-5183" boundStrand="0" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="43"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5169-5179" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="5181-5183" bases="GCC"/></BindingSite><BindingSite simplified="1" location="5171-5185" boundStrand="1" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="46"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5175-5185" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="5171-5173" bases="GCC"/></BindingSite><BindingSite simplified="1" location="5180-2" boundStrand="1" annealedBases="NNNNNNNNNNAGCC" meltingTemperature="41"><Component bases="GGCCACGCGTCGACTAGTAC"/><Component hybridizedRange="5184-2" bases="NNNNNNNNNNA"/><Component bases="C"/><Component hybridizedRange="5180-5182" bases="GCC"/></BindingSite></Primer><Primer recentID="13" name="KROL295 rpsU2_p2_R" sequence="cgcttggcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="236-267" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="cg"/><Component hybridizedRange="265-267" bases="ctt"/><Component bases="g"/><Component hybridizedRange="236-263" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite location="673-682" boundStrand="1" annealedBases="cttaaagcc" meltingTemperature="22"><Component bases="cgcttggcataccctctcacctcg"/><Component hybridizedRange="677-682" bases="cttaaa"/><Component bases="t"/><Component hybridizedRange="673-675" bases="gcc"/></BindingSite><BindingSite simplified="1" location="236-267" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="cg"/><Component hybridizedRange="265-267" bases="ctt"/><Component bases="g"/><Component hybridizedRange="236-263" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="673-682" boundStrand="1" annealedBases="cttaaagcc" meltingTemperature="22"><Component bases="cgcttggcataccctctcacctcg"/><Component hybridizedRange="677-682" bases="cttaaa"/><Component bases="t"/><Component hybridizedRange="673-675" bases="gcc"/></BindingSite></Primer><Primer recentID="14" name="KROL297 rpsU3_p2_R" sequence="ttcttggcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="236-267" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="tt"/><Component hybridizedRange="265-267" bases="ctt"/><Component bases="g"/><Component hybridizedRange="236-263" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite location="673-682" boundStrand="1" annealedBases="cttaaagcc" meltingTemperature="22"><Component bases="ttcttggcataccctctcacctcg"/><Component hybridizedRange="677-682" bases="cttaaa"/><Component bases="t"/><Component hybridizedRange="673-675" bases="gcc"/></BindingSite><BindingSite simplified="1" location="236-267" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="tt"/><Component hybridizedRange="265-267" bases="ctt"/><Component bases="g"/><Component hybridizedRange="236-263" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="673-682" boundStrand="1" annealedBases="cttaaagcc" meltingTemperature="22"><Component bases="ttcttggcataccctctcacctcg"/><Component hybridizedRange="677-682" bases="cttaaa"/><Component bases="t"/><Component hybridizedRange="673-675" bases="gcc"/></BindingSite></Primer><Primer recentID="15" name="KROL164 P035 INT_VSVG" sequence="TTTACCTAATCTATTCATTTCAATATCAG" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="468-496" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="468-496" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite><BindingSite simplified="1" location="468-496" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="468-496" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite></Primer><Primer recentID="16" name="KROL240 katg-ck-1" sequence="gattacgaattcttatttacctaatctat" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="483-500" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="483-500" bases="cttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="483-500" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="483-500" bases="cttatttacctaatctat"/></BindingSite></Primer><Primer recentID="17" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="999-1025" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="999-1025" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="999-1025" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="999-1025" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="18" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="1091-1117" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="1091-1117" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="1091-1117" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="1091-1117" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="19" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="1067-1091" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="1067-1091" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="1067-1091" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="1067-1091" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="20" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:43:15Z"><BindingSite location="4854-4874" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="4854-4874" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="4854-4874" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="4854-4874" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="21" name="KROL324 Tn7_insert_seq" sequence="cctttcgttttatctgttgtttgtcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-07-22T18:39:49Z"><BindingSite location="584-609" boundStrand="1" annealedBases="cctttcgttttatctgttgtttgtcg" meltingTemperature="57"><Component hybridizedRange="584-609" bases="cctttcgttttatctgttgtttgtcg"/></BindingSite><BindingSite simplified="1" location="584-609" boundStrand="1" annealedBases="cctttcgttttatctgttgtttgtcg" meltingTemperature="57"><Component hybridizedRange="584-609" bases="cctttcgttttatctgttgtttgtcg"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>7ab4100a-3115-4fed-ad7c-e0b173353f97</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="15:32:24">2019.12.19</Created>
<LastModified UTC="18:40:50">2020.7.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unsaved Enzyme Set                                                                                         d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI EcoRI KpnI"/></CustomEnzymeSets>
