	   SnapGene      
ctagcaaccgtccgaaatattataaattatcacacacataaaaacagtgctgttaatgtgtctattaagtcgattttttgttataacagacactgcttgtccgatatctgatttaggatacatttntagccacctgtaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattcatatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagccccatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataatcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatctgcagttaaatcgatgtcgacctagctgatcaccgcggccatgatggcataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagtcgatcgtacgctagttcgctccctcactttctggctggatgatggggcgattcangcctggtatgantcagcaacaccttcttcacgangcagacctcactagcaaccgtccgaaatattataaattatcgcacacataaaaacagtgctgttaatgtgtctattaagtcgattttttgttataacagacactgcttgtccgatatttgatttaggatacacgcgatcaagctgacctcgaaccccacgcccctctttaatacgacgggcaatttgcacttcagaaaatgaagagtttgctttagccataacaaaagtccagtatgctttttcacagcataactggactgatttcagtttacaactattctgtctagtttaagactttattgtcatagtttagatctattttgttcagtttaagactttattgtccgcccacacccgcttacgcagggcatccatttattactcaaccgtaaccgattttgccaggttacgcggctggtcctctagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagcgcccaaacataacaggaagaaaaatgccccgctgtgggcggacaaaatagttgggaactgggaggggtggaaatggagtttttaaggattatttagggaagagtgacaaaatagatgggaactgggtgtagcgtcgtaagctaatacgaaaattaaaaatgacaaaatagtttggaactagatttcacttatctggttggcctgcaaggccttcgcgaggtaccgggcccaagcttctcgaggaattcctgcatcattcttctttaggcaaaacattacttaagttttcaaacatagttgattgtaaagccaaatatactttctcttcgaaaacggtgtcgataacttgacctttaggaagttgtccggctatctgtttagaagctgcaatcatagctttaactataggattgttactatttttagctgctgtaaaattacatgcatttttaccaaatagaactctatcagcatcacttacttgcttgtaaatttcattctcagcaatagccttgataaaggcatcaaccatactacttgtagatattaacattaaagtatgacccttaacgtatagtatactatcagaacctattgttgatctaattgcatttgtagctgctttaagtgcccagttgtcgcctaatttatctaggtcgtaaagtataatatcatcaatactagtttgatttagtaagtgtcttagtataggttttacagcttttttatttgatagtctgttaataatatttctagctgtagtaactttggtattagttctgatactgattaagctagcttcacaaattgatagagccgttcttggcgaagctatttttttctgaacactacttaagtcgcctatagtagttactgtatatgtaagtgtgttagcatcagcatatagtgaaactgctttagttgtatcactaccttgaagagacttaatagtctgaatagtcaagtagttagtgatatcgttaaattcaatggcagtagcggcatctttgctattgatgttatattgaccattccaaagaactactcttgcaccccagaacatatcaatgttgcttctgtagtctacatagttaagactagtagcagaatcccatgctgcatttaaatgtgataatactggcttagcaatatcattaaaatacgccttaggaccatttacagttatattttttacgatattaaactgagcgattttctcttcagcataagctttatcaccattttcgataccttttaggatatctattttagtgttaatactttttagtttagcttgttcagcaactactttaattctccagcctaatttcttttgactttcaagttttttgatgttgttattaatctcatctctttcagctctgtatttctcaagaccttgagcagcagttgcttgatctttcatatacatattttttggcttgatagagttcggtatcttaacagcaccattgttagttaagacaaaacctctagctaatttttctccatttgcttgaatattacttgcatcagctgctatgcttttgaatgcataaattggtgaatttgtattagtatcagcatctgtctcaacattattagaatatttagtaacagattcatttacaatttttgcatttaaatcatcagcgtaaactgaagttgttgcgatagttcctaaaagagcgatagttagtgtctttttcatttttgtctccttgacttgttttaaaaaaattattatttgttttaaaatttaacaaaattttacttaatattaattttaagtcaatatttaaattaatattttattcatttattttatattttattaatatttaaatatatatttaataaaaacttgtttgataacattagaaataaaaggattttggtgattaatatttaattaattttatgatgaatgcccgggggatccactagtgagctcatgcatgatatcgtccgggccgcaagctcctagcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctcaagctagagagtcattaccccaggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccctgccactcatcgcagtctagcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagctcagctaattaag  8u         
                                                   "      !         "   )   "                  
                               &                                    "      $      $                                                 .            %                     E   4          3   &         (   !                     ,   ,         -   N                                                #                                    8            #            $            '         -   ,            A                     -   &   !      A   (   "   0   F   s   ,      %   (                                                                                                                                       $      	               &                     $      /                                                                        ]

Q Df^

zFMq
 |B	N	R 	5X &u


C4{N\  EgK_k
  GVrv s lm]	 

?In&~gs )x
V9 RP$	C	uS`,z }w%%?j		)D O+@O		x> 	 	F6JO S	$

-Y 5ptK V9\
g3EP Tv
DO   v	1
Lc|? 'XJ	x	 
M
]vA"   6,		


-U5\? ~7		}

xAw!swM!   FhLPA`	'	
e~!$WZj
 9	3

d /V  AlX		
9GH]] 3"  mEH&NUr l1		!

@	Y1) f t +47Q
J\Nf0;|`" G m  -\s n{.NRvh=%et} _c:	^	o	q


o{'i )	I	
 X  )
k

:z   *Ox	;YI )x-y
W4=nC
 QL		|3T %	O

vZxz-{ 9 mra	 . b;SeD	=
" 
tz-xtH^  )0+	k	-

*EA_  ~x&&@		?MR}z5; =^
 >?		
PC:zf?\ 3 O  Pjgw,P	}lwT_&  EZ5I^rAx	z		y:_-R\(R  m	?`D" D tux	cj wX2L
0C ; KGoDKG 	 b =i} #=ih		Z
G
17 Z]7K .
XjP g	aWi Bg*:
SRYa   7		

.,	'"  2KoU!5Lr	
%
$rp#Aw SUTF		
$+Z$ 0 p6~d!	4	?

qu
M0{m 	
/^+P;$ ^   
&L0p~/B PQg	cXu Wo		#wA  8a|B
]

k(F   :
	

4FQm;!4f 	h++v{ yPpW r %w

Umfur v*OtC UEN   w		0
,Tu>>@ }O			
2
d}#\2cUi   ,b0	9	n	Mz[M/d W  ,NwH:3X - aK	<
N
qw (]*	
,
>Q
s]:^.  DYi4H]qv>	y		O9|B^,9yvSQ C Fsw
iJB -g	
V	6Wh  1n6 		

#Q+`&@%   }

%./]z*.Ot:  7 	{
v*E@:  $~
lBxeqV   +NMv/	8	m			/
y+"tW B  Ev)		N8+x=hP 0z

"gD)N9  #M
(	.		
MkfC<pE ' (           i[\	-	
Wj"#%&=}[TUoklmn4      	`Q=mBKab'>X~I6v'}Eko  )  Gj]	
X$ 3\V 6 
2Rj/=b		


(Y<w 3    :C:7c?JW>FQl_&    a>L4
3en72?(0pp  Y.bnbee~ AH 		n


:%.]J( * T   gkB^d	T


0!46^4Lac!  %;Y		`

	,Y5GHIi!)Wb  9mG


 ^ jz M3~#P>"


AZ 4F'7V5			'
K

aA 7 sk0c	
^
=xZ  nISO!m2	8

)k!2 [t(8@7O<qQ,  % R 	W,;IY#8;E
R
K g}%Ram`,  g  2;x!5DNBDK17X 4?GIK u  	
F]dOM$T   (?U~	}&O>co8)-   ? ]z	FMs	5
4i$39&b7OR;f~FqN H n y   t4!J|/h(B"t| 	S_fltSr@|1Zjqy	"+/6AMu- .=bqdo?	w3f'  oPU	r

  -;	
(Y9= i  Zcd7c_	
pjfkC 9`/p		|of B
<^ *%
H	o



; IJ	
K 3		n&/t + U l_e	
*
(Ub    !C9`l


1 ;{4n # w hWS	
\"575OMd  ! Y UG	U

&B_ [ Z&+Z	W
<-HfIJ"zc 3<	
&Va"Z*#   PyV		a
e
[HZ'Oj)d-JX  ?SzW	

 >6%  j:Iz


D *W
H


X52_!\  y.>Qo_ n	D-I {A	M	

4EL  kJ(\	>B3}U[ N$Q?#		w(+y 48R	#

sJDOI  5Wu			L
K[b  @8&	6	

w\.8 l rDG%*3M-k6	P

Qu<h$:{X_|0   (		(
j

ymBH 8 l:d	{2y  

s@,w|Gy4# 2 N   =flw	_e>
![^[ : a tnCW1/hb|F \"<fY
`i   JTAfTE	9

R#q*o
"v$ / ] OoPcf	3		>
LW}Al `n	A

"*3e q )sot  |aG9 L.=Th=' G\up{A8P	ruIR\9]  ~ |5
$uy-_ A  *uLuyl	.
!d*(gVU-  &   _
<lJZ,		LViB;u&|DSnj, 5 1X-ai.9<6d	m~1>d=P S 
$F
S


+Fh5I`ba 3y"6O	7
`@  Q wH'CELNSp~3HJA  > <y'Er>Tg}'A!s{			|k2bRa{6QY!&.5:@L,  h  8O	nX8< 2
;s v  8_R		ATNm >R
d
=Ge&N%(U!cy$, 
1^PH  @		
'2DH*x-     $?)Lt			
P;lg9GW^/& M `   Vkv?{+dv ]3Z!  N_@		
Q~!KVp)@	u p {`FK~ns8S\gA 4 @  ^khtKtx-	5 Uchc)4:H'fUt0c{ <COTi(   P v7GNS|&z	6
{mj`P%-29+" Q
71Q	
c
<:%xlGr0 L    #^Kju		
  JUu FV2t]w.F o u66JR_s{%y	z;JTbg9bmrs/[kz$,18BN*Ss I J K        5"][\}~i)#$X}~			{			


`a:;78IT`a.TUVWESTUrs
#07v),  F]0*CBY|		<d/QXALp}$ Z}	W%% />pG"1nD\N01#Mqg((   e ^@+C		g
]maxR EYD4B~ c%r		(l=r@e [	s
e E  RSpvyV	Xd u
 
& Q~		
k  q2o[M<		1OZ$r>  ?.b	)1:NJ7 0|HY3
@] ih)D  Uhx#1E	

Z2 j HdA8di	h
^yZEwD <   _pEe"qnkL   L#[ D
8bUF'Cn f,Y`		S5glH 
 c &:>m>~ ds	7 A0.		q	}jAf a	)spg Ce\	 lj
t

H%8 $>i			=_ [
,fo2  S+I	
   @W		|
@8FvYL	 F e [^Tq zp

e (w
< 
!Y </	yL 	
 kC'Q  Jv<K Yh	0 R4Kq	X  	
b	o/ 			
j'luz uNTM V +		
2SZ[1bc  , rCh`3p \=	

+
;
  m~f	P).V*%9s?  /D	7*O8    "
a<|2H5  a	
2
;o^  @f8:c		_
m



/4-(K iL}4&
J


]j $  1:VX"ZMs	D

A6#6P$$ x 3pcI%^#8sqNix*eE ,6T	
jB	  $G	
m    " TVF

%'[3 Z Yy2VU		V
Z`  ViH	


C,[ 7I
'"	[	=rvICNK& k qC2,j5	i

Jt#[ 1  <eB9	
.gz1{FxE \ [ee	@
X
_h
|nd~    {4F#ro,[<  	`
l=  = q=f r		_WaQo 0@S'b#M+$ _     Ms		
1k*G(Y 0E
b
(Geh/  \QzW	
I96HScPq.DKo# $!	f
'\k[MlqV0C QR	
d	  Tg{0Z	a		
?YhI6 "Xh!7T&mv @-6 dk	
+n$  d '?	{
?Eu   ;;	xB XJp	
.y t}		
-)08L e	^

63`G] 9+bC
I

"i  X
Y	l  B;1	
	
Bgs	} zq?R`n<  /	r	
j~k5GRpF"g !/5 c		*t-x 	,/hK pirE| bo	q,.Jw   zTTTN   {TTNT   |TNTA   }NTAG  TCAN  CANG  ANGC  NGCC  TGAN  GANT  ANTC  NTCA  &CGAN  'GANG  (ANGC  )NGCA  *6  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    	                                                                                                                                                                              	                                           
                      	                                          
                                                                                         	                     
         
                                                                                                                           
                     &                     \   ]         &         A   M                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     	                                                                    	                 
        	              
                                 	       
   
                                           
       &       \ ]   &   A M   o1  o  o  p  r  v  {Oxx5G,-GV ?	g	
hD	L
Lh(r~ D

6q>X	Z 'PryAq
7	x P GIuRK
Y	
[
  `/5	MI	(e\ZSD		"SD	"Qc4b;~W+SD	"XoG	s Je
SoFhbk
>Y6s	6p
bcKoj

,	p
SD		"X3\R"Ts6|q(M+\b]	n	nz:
Z YV
c	TgszBF6f 5	M6P IS!1Muu5SD	:"RK   AH0#3)sd&f	.dQeuQs	(e6 CW>y
		A	]
~ F
N ]	
n&_o	{t	BLG?
{mx
Vgcd	*$	u,zUh	
x
V?
{m+!
@wAWo	?
Z
1{mcdt		*BL5
A mHN l1	
1&Z	<HJ	
l 
?Z	J + :_*
-f<^z ZE	J, `;
Z YV
	-x -nvdeA

 c j &>i	h
s
1>ZZ	jk}
AwPwP	


*Exw_~7		x!s# UA w

x-nvdeWo	Z
1, Z Y`;
V
7
/G\g(
:Yh	0XJp	
.y#=ih		Z
G
17=^
  }

%./]z*.Ot:g	aWi   {4F#ro,[<_c:	^	o	q


o{'iV9\
g3EPdk	
+n$iL}4&
J


]j   7V59} 	{\



%gpt.Kv*/]3EPEz*.@O:t : $ Q  13:iwyLV}"6HX"ZMOs'4		&
7
D
J



ACE`]6L#@NSp~6Pj$3HJIJ	
K  JTAfTE	9

R#q*o
"v   a>L4
3en72?(0pp!/5Tv
DO4 @  ^khtKtx-	5 Uchc)4:H'fUt0c{ <COTi   Ivy}.*JO	


%t.C/>Q]oK_z*.Ot:E  RSpvyV	Xd  Ht        }   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     S4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:AkO6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="57"><Feature recentID="56" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="55" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="54" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="53" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="52" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="51" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="50" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="49" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="48" name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="47" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="37" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="36" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="35" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="34" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="33" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="32" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="31" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="30" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="25" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="24" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="16" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="9" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="8" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="7" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="6" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4733" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="5" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="4" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="3" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="2" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4404" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="1" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4427-4524" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="0" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4621-4715" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>97da295a-ac06-4ba6-951b-43fb4ef45dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="16:44:49">2019.10.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
  x<?xml version="1.0"?><HistoryTree><Node name="pMP749_(modified (1).dna" type="DNA" seqLen="6409" ID="1" circular="1" operation="insert"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4367..6042" colors="red"/></TopStrand><BottomStrand><ColorRange range="4367..6042" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4367"/><Node name="pMP749_(modified (1).dna" type="DNA" seqLen="4733" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="3" type="misc_feature" allowSegmentOverlaps="0"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="1" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="2" type="misc_feature" allowSegmentOverlaps="0"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0"><Segment range="2873-3115" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4147-4733" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0"><Segment range="2258-2872" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0"><Segment range="309-1124" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature></Features></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  r<?xml version="1.0"?><Features nextValidID="99"><Feature recentID="98" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4368-6043" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="97" name="RPp7" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4368-4377" color="#0080ff" type="standard"/><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="96" name="RPp4" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5817-5843" color="#0080ff" type="standard"/><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="95" name="PRP12" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5975-6004" color="#8080ff" type="standard"/><Q name="note"><V text="PRP12"/></Q></Feature><Feature recentID="94" name="200 bp max AT content (91%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5844-6043" color="#0080ff" type="standard"/><Q name="note"><V text="200 bp max AT content (91%)"/></Q></Feature><Feature recentID="93" name="150 bp max AT content (93.3%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5844-5993" color="#0080ff" type="standard"/><Q name="note"><V text="150 bp max AT content (93.3%)"/></Q></Feature><Feature recentID="92" name="100 bp max AT content (95%)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5865-5964" color="#0080ff" type="standard"/><Q name="note"><V text="100 bp max AT content (95%)"/></Q></Feature><Feature recentID="91" name="Potential TSS" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5875-5875" color="#ff8080" type="standard"/><Q name="note"><V text="Potential TSS"/></Q></Feature><Feature recentID="90" name="Potential -10 element" directionality="2" type="-10_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5882-5887" color="#00ff00" type="standard"/><Q name="note"><V text="Potential -10 element"/></Q></Feature><Feature recentID="89" name="Potential -35 element" directionality="2" type="-35_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5904-5909" color="#00ff00" type="standard"/><Q name="note"><V text="Potential -35 element"/></Q></Feature><Feature recentID="88" name="P431 P_FTL0702_F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5975-6004" color="#50ff50" type="standard"/><Q name="note"><V text="P431 P_FTL0702_F"/></Q></Feature><Feature recentID="87" name="P434 FTL0702cmp_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5843-5876" color="#50ff50" type="standard"/><Q name="note"><V text="P434 FTL0702cmp_F2"/></Q></Feature><Feature recentID="86" name="FTL_0702" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4368-5825" color="#00e600" type="standard"/></Feature><Feature recentID="85" name="hypothetical protein" directionality="2" translationMW="53302.96" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4368-5825" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKTLTIALLGTIATTSVYADDLNAKIVNESVTKYSNNVETDADTNTNSPIYAFKSIAADASNIQANGEKLARGFVLTNNGAVKIPNSIKPKNMYMKDQATAAQGLEKYRAERDEINNNIKKLESQKKLGWRIKVVAEQAKLKSINTKIDILKGIENGDKAYAEEKIAQFNIVKNITVNGPKAYFNDIAKPVLSHLNAAWDSATSLNYVDYRSNIDMFWGARVVLWNGQYNINSKDAATAIEFNDITNYLTIQTIKSLQGSDTTKAVSLYADANTLTYTVTTIGDLSSVQKKIASPRTALSICEASLISIRTNTKVTTARNIINRLSNKKAVKPILRHLLNQTSIDDIILYDLDKLGDNWALKAATNAIRSTIGSDSILYVKGHTLMLISTSSMVDAFIKAIAENEIYKQVSDADRVLFGKNACNFTAAKNSNNPIVKAMIAASKQIAGQLPKGQVIDTVFEEKVYLALQSTMFENLSNVLPKEE*"/></Q></Feature><Feature recentID="84" name="P364 FTL0702cmp_F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5799-5825" color="#8080ff" type="standard"/><Q name="note"><V text="P364 FTL0702cmp_F"/></Q></Feature><Feature recentID="83" name="P365 FTL0702cmp_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4371-4398" color="#8080ff" type="standard"/><Q name="note"><V text="P365 FTL0702cmp_R"/></Q></Feature><Feature recentID="82" name="P367 FTL0702seqF1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5494-5518" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="81" name="P368 FTL0702seqR1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4650-4678" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="80" name="P411 FTL0702sp_2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4723-4747" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="79" name="P429 FTL0702_VSVG_2R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5395-5421" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="78" name="P428 FTL0702_VSVG_2F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4371-4398" color="#50ff50" type="standard"/><Q name="note"><V text="P428 FTL0702_VSVG_2F"/></Q></Feature><Feature recentID="77" name="P433 FTL0702cmp_R2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4368-4395" color="#50ff50" type="standard"/><Q name="note"><V text="P433 FTL0702cmp_R2"/></Q></Feature><Feature recentID="76" name="P435 RT_FTL0702-1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5214-5233" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="75" name="P436 RT_FTL0702-1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5114-5135" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="74" name="P437 RT_FTL0702-2F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4578-4600" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="73" name="P438 RT_FTL0702-2R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4474-4495" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="72" name="P439 RT_FTL0702-3F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4995-5016" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="71" name="P440 RT_FTL0702-3R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4931-4950" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="70" name="P444 pKL75_F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4884-4910" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="69" name="P692 PriM_mpk_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5172-5198" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="68" name="P693 PriM_mpk_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5119-5147" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="67" name="P698 Ck_PriM_mpk1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4735-4757" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="66" name="P697 Ck_PriM_mpk1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5210-5233" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="65" name="P700 PriM_mtip1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5466-5493" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="64" name="P701 PriM_mtip1_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5416-5441" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="63" name="P702 PriM_mtip1_R2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4431-4455" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="62" name="P703 CkPriM_mtip1F" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5732-5761" color="#50ff50" type="standard"/><Q name="note"><V text="P703 CkPriM_mtip1F"/></Q></Feature><Feature recentID="61" name="P704 CkPriM_mtip1R" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5262-5288" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="60" name="P706 PriM_mtip2_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5466-5493" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="59" name="P705 PriM_mtip2_F2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5400-5426" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="58" name="P707 PriM_unst1_R1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5179-5205" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="57" name="P708 PriM_unst1_F1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5144-5168" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="56" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="55" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="54" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="53" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="52" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="51" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="50" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="49" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="48" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="47" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="37" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="36" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="35" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="34" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="33" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="32" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="31" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="30" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQL"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="25" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="24" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"/></Q></Feature><Feature recentID="17" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="16" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="9" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="8" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="7" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="6" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-6409" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="5" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="4" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="3" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="2" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-6080" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="1" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6103-6200" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="0" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6297-6391" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature></Features>
  a<?xml version="1.0"?><Primers recycledIDs="43" nextValidID="53"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="52" name="P820 PriM_mtip1_F3" sequence="atatGGATCCtaaaacaaataataatttttttaaaacaagtc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5837-5868" boundStrand="1" annealedBases="taaaacaaataataatttttttaaaacaagtc" meltingTemperature="50"><Component bases="atatGGATCC"/><Component hybridizedRange="5837-5868" bases="taaaacaaataataatttttttaaaacaagtc"/></BindingSite><BindingSite simplified="1" location="5837-5868" boundStrand="1" annealedBases="taaaacaaataataatttttttaaaacaagtc" meltingTemperature="50"><Component bases="atatGGATCC"/><Component hybridizedRange="5837-5868" bases="taaaacaaataataatttttttaaaacaagtc"/></BindingSite></Primer><Primer recentID="51" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4875-4901" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="4875-4901" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="4875-4901" boundStrand="0" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="4875-4901" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="50" name="P818 PriM_mpk_F2" sequence="atatGGATCCaattgtaaatgaatctgttactaaatattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5718-5747" boundStrand="1" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="5718-5747" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite><BindingSite simplified="1" location="5718-5747" boundStrand="1" annealedBases="aattgtaaatgaatctgttactaaatattc" meltingTemperature="52"><Component bases="atatGGATCC"/><Component hybridizedRange="5718-5747" bases="aattgtaaatgaatctgttactaaatattc"/></BindingSite></Primer><Primer recentID="49" name="P819 PriM_mpk_R2" sequence="atatGGTACCtgtaaagccaaatatactttctcttcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4416-4442" boundStrand="0" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4416-4442" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite><BindingSite simplified="1" location="4416-4442" boundStrand="0" annealedBases="tgtaaagccaaatatactttctcttcg" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4416-4442" bases="tgtaaagccaaatatactttctcttcg"/></BindingSite></Primer><Primer recentID="48" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5158-5204" boundStrand="0" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="5158-5167" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="5171-5176" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="5178-5204" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="5158-5204" boundStrand="0" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="5158-5167" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="5171-5176" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="5178-5204" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="47" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5143-5187" boundStrand="1" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="57"><Component hybridizedRange="5178-5187" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="5171-5176" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="5143-5167" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="5143-5187" boundStrand="1" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="57"><Component hybridizedRange="5178-5187" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="5171-5176" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="5143-5167" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="46" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="5465-5492" boundStrand="0" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="5465-5492" boundStrand="0" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="45" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="5399-5426" boundStrand="1" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="55"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="5399-5426" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="5399-5426" boundStrand="1" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="55"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="5399-5426" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="44" name="P428 FTL0702_VSVG_2F" sequence="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGCttcttctttaggcaaaacattacttaag" description="0"><BindingSite location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="TAATCTATTCATTTCAATATCAGTATAtGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="42" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="5442-5492" boundStrand="0" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="5442-5446" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="5447-5458" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="5447-5492" boundStrand="0" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="5447-5458" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="5465-5492" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="41" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="5118-5167" boundStrand="1" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="5162-5167" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="5150-5159" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="5118-5146" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="5118-5167" boundStrand="1" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="5162-5167" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="5150-5159" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="5118-5146" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="40" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="5415-5458" boundStrand="1" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="5447-5458" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="5443-5446" bases="AAGA"/><Component hybridizedRange="5415-5441" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="5415-5458" boundStrand="1" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="5447-5458" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="5415-5442" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="39" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="5150-5197" boundStrand="0" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="5150-5159" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="5162-5167" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="5171-5197" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="5150-5197" boundStrand="0" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="5150-5159" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="5162-5167" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="5171-5197" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="38" name="P434 FTL0702cmp_F2" sequence="atatCCCGGGtaaattttaaaacaaataataatttttttaaaac" description="Contains XmaI site"><BindingSite location="5842-5875" boundStrand="1" annealedBases="taaattttaaaacaaataataatttttttaaaac" meltingTemperature="48"><Component bases="atatCCCGGG"/><Component hybridizedRange="5842-5875" bases="taaattttaaaacaaataataatttttttaaaac"/></BindingSite><BindingSite simplified="1" location="5842-5875" boundStrand="1" annealedBases="taaattttaaaacaaataataatttttttaaaac" meltingTemperature="48"><Component bases="atatCCCGGG"/><Component hybridizedRange="5842-5875" bases="taaattttaaaacaaataataatttttttaaaac"/></BindingSite></Primer><Primer recentID="37" name="P365 FTL0702cmp_R1" sequence="atatGCGGCCGCttcttctttaggcaaaacattacttaag" description="Contains NotI site"><BindingSite location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="4370-4397" boundStrand="0" annealedBases="ttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="36" name="P431 compPFTL0702_F" sequence="atatCCCGGGcttttatttctaatgttatcaaacaagttt" description="Contains XmaI site"><BindingSite location="5974-6003" boundStrand="1" annealedBases="cttttatttctaatgttatcaaacaagttt" meltingTemperature="53"><Component bases="atatCCCGGG"/><Component hybridizedRange="5974-6003" bases="cttttatttctaatgttatcaaacaagttt"/></BindingSite><BindingSite simplified="1" location="5974-6003" boundStrand="1" annealedBases="cttttatttctaatgttatcaaacaagttt" meltingTemperature="53"><Component bases="atatCCCGGG"/><Component hybridizedRange="5974-6003" bases="cttttatttctaatgttatcaaacaagttt"/></BindingSite></Primer><Primer recentID="35" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="4367-4394" boundStrand="0" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="4367-4394" bases="tcattcttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="4367-4394" boundStrand="0" annealedBases="tcattcttctttaggcaaaacattactt" meltingTemperature="55"><Component bases="atatGGATCC"/><Component hybridizedRange="4367-4394" bases="tcattcttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="34" name="P366 FTL0702cmp_R2" sequence="atatGGATCCttcttctttaggcaaaacattacttaag" description="&quot;Contains BamHI site, could use to make vector lacking VSVG tag&quot;"><BindingSite location="4363-4397" boundStrand="0" annealedBases="tGATCttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="ata"/><Component hybridizedRange="4363-4364" bases="tG"/><Component bases="G"/><Component hybridizedRange="4366-4368" bases="ATC"/><Component bases="C"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite><BindingSite simplified="1" location="4363-4397" boundStrand="0" annealedBases="tGATCttcttctttaggcaaaacattacttaag" meltingTemperature="54"><Component bases="ata"/><Component hybridizedRange="4363-4364" bases="tG"/><Component bases="G"/><Component hybridizedRange="4366-4368" bases="ATC"/><Component bases="C"/><Component hybridizedRange="4370-4397" bases="ttcttctttaggcaaaacattacttaag"/></BindingSite></Primer><Primer recentID="33" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="4883-4909" boundStrand="1" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4883-4909" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="4883-4909" boundStrand="1" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="4883-4909" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="32" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="5008-5044" boundStrand="0" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="5008-5014" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="5018-5044" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="5008-5044" boundStrand="0" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="5008-5014" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="5018-5044" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="31" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="5394-5420" boundStrand="1" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="5394-5420" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="5394-5420" boundStrand="1" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="5394-5420" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="30" name="P364 FTL0702cmp_F" sequence="atatCCCGGGatgaaaaagacactaactatcgctctt" description="Contains XmaI site"><BindingSite location="5798-5824" boundStrand="1" annealedBases="atgaaaaagacactaactatcgctctt" meltingTemperature="58"><Component bases="atatCCCGGG"/><Component hybridizedRange="5798-5824" bases="atgaaaaagacactaactatcgctctt"/></BindingSite><BindingSite simplified="1" location="5798-5824" boundStrand="1" annealedBases="atgaaaaagacactaactatcgctctt" meltingTemperature="58"><Component bases="atatCCCGGG"/><Component hybridizedRange="5798-5824" bases="atgaaaaagacactaactatcgctctt"/></BindingSite></Primer><Primer recentID="29" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="4989-5014" boundStrand="1" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="57"><Component bases="ttcactaGCA"/><Component hybridizedRange="4989-5014" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="4989-5014" boundStrand="1" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="57"><Component bases="ttcactaGCA"/><Component hybridizedRange="4989-5014" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="28" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="4430-4454" boundStrand="0" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="59"><Component bases="atatGGTACC"/><Component hybridizedRange="4430-4454" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="4430-4454" boundStrand="0" annealedBases="tactttctcttcgaaaacggtgtcg" meltingTemperature="59"><Component bases="atatGGTACC"/><Component hybridizedRange="4430-4454" bases="tactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="27" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="4722-4746" boundStrand="0" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="62"><Component bases="atatGGTACC"/><Component hybridizedRange="4722-4746" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="4722-4746" boundStrand="0" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="62"><Component bases="atatGGTACC"/><Component hybridizedRange="4722-4746" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="26" name="P410 FTL0702sp30_2F" sequence="taaatTaatctgttactaaatattctaataatg" description="0"><BindingSite location="5710-5736" boundStrand="1" annealedBases="aatctgttactaaatattctaataatg" meltingTemperature="49"><Component bases="taaatT"/><Component hybridizedRange="5710-5736" bases="aatctgttactaaatattctaataatg"/></BindingSite><BindingSite simplified="1" location="5710-5736" boundStrand="1" annealedBases="aatctgttactaaatattctaataatg" meltingTemperature="49"><Component bases="taaatT"/><Component hybridizedRange="5710-5736" bases="aatctgttactaaatattctaataatg"/></BindingSite></Primer><Primer recentID="25" name="P409 FTL0702sp30_1R" sequence="acagattAatttacaatttttgcatttaaatc" description="0"><BindingSite location="5730-5761" boundStrand="0" annealedBases="acagattatttacaatttttgcatttaaatc" meltingTemperature="51"><Component hybridizedRange="5730-5736" bases="acagatt"/><Component bases="A"/><Component hybridizedRange="5738-5761" bases="atttacaatttttgcatttaaatc"/></BindingSite><BindingSite simplified="1" location="5730-5761" boundStrand="0" annealedBases="acagattatttacaatttttgcatttaaatc" meltingTemperature="51"><Component hybridizedRange="5730-5736" bases="acagatt"/><Component bases="A"/><Component hybridizedRange="5738-5761" bases="atttacaatttttgcatttaaatc"/></BindingSite></Primer><Primer recentID="24" name="P703 CkPriM_mtip1F" sequence="atttaaatgcaaaaattgtaaatgaatctg" description="0"><BindingSite location="5731-5760" boundStrand="1" annealedBases="atttaaatgcaaaaattgtaaatgaatctg" meltingTemperature="54"><Component hybridizedRange="5731-5760" bases="atttaaatgcaaaaattgtaaatgaatctg"/></BindingSite><BindingSite simplified="1" location="5731-5760" boundStrand="1" annealedBases="atttaaatgcaaaaattgtaaatgaatctg" meltingTemperature="54"><Component hybridizedRange="5731-5760" bases="atttaaatgcaaaaattgtaaatgaatctg"/></BindingSite></Primer><Primer recentID="23" name="P415 FTL0702sp17_2F" sequence="caacttAagtttacgctgatgatttaaatg" description="0"><BindingSite location="5752-5781" boundStrand="1" annealedBases="caacttagtttacgctgatgatttaaatg" meltingTemperature="53"><Component hybridizedRange="5776-5781" bases="caactt"/><Component bases="A"/><Component hybridizedRange="5752-5774" bases="agtttacgctgatgatttaaatg"/></BindingSite><BindingSite simplified="1" location="5752-5781" boundStrand="1" annealedBases="caacttagtttacgctgatgatttaaatg" meltingTemperature="53"><Component hybridizedRange="5776-5781" bases="caactt"/><Component bases="A"/><Component hybridizedRange="5752-5774" bases="agtttacgctgatgatttaaatg"/></BindingSite></Primer><Primer recentID="22" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="4649-4677" boundStrand="0" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="4649-4677" boundStrand="0" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="21" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="4649-4677" boundStrand="1" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="4649-4677" boundStrand="1" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="4649-4677" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="20" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="5261-5287" boundStrand="0" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="5261-5287" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="5261-5287" boundStrand="0" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="5261-5287" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="19" name="P414 FTL0702sp17_1R" sequence="gtaaactTaagttgttgcgatagttcc" description="0"><BindingSite location="5768-5794" boundStrand="0" annealedBases="gtaaactaagttgttgcgatagttcc" meltingTemperature="52"><Component hybridizedRange="5768-5774" bases="gtaaact"/><Component bases="T"/><Component hybridizedRange="5776-5794" bases="aagttgttgcgatagttcc"/></BindingSite><BindingSite simplified="1" location="5768-5794" boundStrand="0" annealedBases="gtaaactaagttgttgcgatagttcc" meltingTemperature="52"><Component hybridizedRange="5768-5774" bases="gtaaact"/><Component bases="T"/><Component hybridizedRange="5776-5794" bases="aagttgttgcgatagttcc"/></BindingSite></Primer><Primer recentID="18" name="P412 CkFTL0702stp30F" sequence="tcaaggagacaaaaatgaaaaagacac" description="0"><BindingSite location="5812-5838" boundStrand="1" annealedBases="tcaaggagacaaaaatgaaaaagacac" meltingTemperature="57"><Component hybridizedRange="5812-5838" bases="tcaaggagacaaaaatgaaaaagacac"/></BindingSite><BindingSite simplified="1" location="5812-5838" boundStrand="1" annealedBases="tcaaggagacaaaaatgaaaaagacac" meltingTemperature="57"><Component hybridizedRange="5812-5838" bases="tcaaggagacaaaaatgaaaaagacac"/></BindingSite></Primer><Primer recentID="17" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="5493-5517" boundStrand="1" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="5493-5517" boundStrand="1" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="16" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="5493-5517" boundStrand="0" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="5493-5517" boundStrand="0" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="5493-5517" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="15" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="5209-5232" boundStrand="1" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="5209-5232" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="5209-5232" boundStrand="1" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="5209-5232" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="14" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="4577-4599" boundStrand="1" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="4577-4599" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="4577-4599" boundStrand="1" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="4577-4599" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="13" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="4734-4756" boundStrand="0" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="64"><Component hybridizedRange="4734-4756" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="4734-4756" boundStrand="0" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="64"><Component hybridizedRange="4734-4756" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="12" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="4473-4494" boundStrand="0" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="4473-4494" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="4473-4494" boundStrand="0" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="4473-4494" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="11" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="4994-5015" boundStrand="1" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="56"><Component hybridizedRange="4994-5015" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="4994-5015" boundStrand="1" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="56"><Component hybridizedRange="4994-5015" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="10" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="5113-5134" boundStrand="0" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="5113-5134" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="5113-5134" boundStrand="0" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="5113-5134" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="9" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="4930-4949" boundStrand="0" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="4930-4949" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="4930-4949" boundStrand="0" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="4930-4949" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="8" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="5213-5232" boundStrand="1" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="5213-5232" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="5213-5232" boundStrand="1" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="5213-5232" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="7" name="KROL19 PriMnoCFR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5649-5675" boundStrand="1" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="5649-5675" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="5649-5675" boundStrand="1" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="5649-5675" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="6" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4919-4943" boundStrand="0" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="4919-4943" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="4919-4943" boundStrand="0" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="4919-4943" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer><Primer recentID="5" name="KROL21 PriMnoC1FR2F" sequence="atatGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4883-4916" boundStrand="1" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component bases="atatGC"/><Component hybridizedRange="4883-4916" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="4883-4916" boundStrand="1" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component bases="atatGC"/><Component hybridizedRange="4883-4916" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="4" name="KROL23 PriMnoC2FR2F" sequence="aaaaatgcaGCtaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4525-4564" boundStrand="1" annealedBases="aaaaatgcataattttacagcagctaaaaatagtaaca" meltingTemperature="54"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GC"/><Component hybridizedRange="4525-4553" bases="taattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="4525-4564" boundStrand="1" annealedBases="aaaaatgcataattttacagcagctaaaaatagtaaca" meltingTemperature="54"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GC"/><Component hybridizedRange="4525-4553" bases="taattttacagcagctaaaaatagtaaca"/></BindingSite></Primer><Primer recentID="3" name="KROL149 Ck_pKR4_F" sequence="acggtgtcgataacttgacctttag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T14:03:34Z"><BindingSite location="4446-4470" boundStrand="0" annealedBases="acggtgtcgataacttgacctttag" meltingTemperature="59"><Component hybridizedRange="4446-4470" bases="acggtgtcgataacttgacctttag"/></BindingSite><BindingSite simplified="1" location="4446-4470" boundStrand="0" annealedBases="acggtgtcgataacttgacctttag" meltingTemperature="59"><Component hybridizedRange="4446-4470" bases="acggtgtcgataacttgacctttag"/></BindingSite></Primer><Primer recentID="2" name="KROL150 Ck_pKR4_R" sequence="gatatcctaaaaggtatcgaaaatggt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T14:09:25Z"><BindingSite location="5351-5377" boundStrand="1" annealedBases="gatatcctaaaaggtatcgaaaatggt" meltingTemperature="55"><Component hybridizedRange="5351-5377" bases="gatatcctaaaaggtatcgaaaatggt"/></BindingSite><BindingSite simplified="1" location="5351-5377" boundStrand="1" annealedBases="gatatcctaaaaggtatcgaaaatggt" meltingTemperature="55"><Component hybridizedRange="5351-5377" bases="gatatcctaaaaggtatcgaaaatggt"/></BindingSite></Primer><Primer recentID="1" name="KROL152 Ck_pKR5_R" sequence="taactacttgactattcagactattaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T14:38:00Z"><BindingSite location="5057-5084" boundStrand="1" annealedBases="taactacttgactattcagactattaag" meltingTemperature="53"><Component hybridizedRange="5057-5084" bases="taactacttgactattcagactattaag"/></BindingSite><BindingSite simplified="1" location="5057-5084" boundStrand="1" annealedBases="taactacttgactattcagactattaag" meltingTemperature="53"><Component hybridizedRange="5057-5084" bases="taactacttgactattcagactattaag"/></BindingSite></Primer><Primer recentID="0" name="KROL35 PriMnoC2FR2F" sequence="aaaaatgcaGCAaattttacagcagctaaaaatagtaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T14:52:12Z"><BindingSite location="4525-4564" boundStrand="1" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="4525-4552" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite><BindingSite simplified="1" location="4525-4564" boundStrand="1" annealedBases="aaaaatgcaaattttacagcagctaaaaatagtaaca" meltingTemperature="53"><Component hybridizedRange="4556-4564" bases="aaaaatgca"/><Component bases="GCA"/><Component hybridizedRange="4525-4552" bases="aattttacagcagctaaaaatagtaaca"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>592d25e9-94f4-4455-bdb6-bd223d45f268</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="16:44:49">2019.10.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                    HO  d               E              Y?                                                                                                                                                                <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI EcoRI"/></CustomEnzymeSets>
