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UbH a {  F`   IR&		
\m6:I P}.M+		
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d

6h  L9c~fI	 R%Xs ) N		hb2 s 
1 f y"	|   f 

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6Ttd0 RZ] y :R	
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v*E[tv MM
m

#WDq GR?g  Z`rg=W	"	I wdme		\+@  yF	u	 Aj	
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2*L"  	z\kp0
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Ox VD/S :0:	[	n		
1oxO H0]B	
OL 2:@ % '4q					
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p61 8 E RA?:

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r m1B		
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. SYVB?A	  it
sJ# S
T'I	<

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Ju	


]47;  eP CN	I6i Y			C
e

 JpTZJi 6 n~/29,		
7.YfJ  Q		
Wd    *p/i~6MJ	  0%	g   #L,:5V	 d#dJ   .r7Tb?kt;    _}Yr   'V"FS1Yrg&	&D 	   H^&H\,	 ?3L]		gHc *   O	i-b _ } -OWVZ3 ; w P		  5}L	 c vBIN H fa33	
 / t <gTgH2  FTDZ\Ft@G	$	1	TB g<8b			 g  ,im   7ZWCkz#B		K}!   Ly*n:gjV5 !  &
t%%- 3 [  pl'
iBQ   C '*i>od	^	u
K J
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7o&ze$6tSW{ T			
u5B^  	a	US[  x

b-< v;>	g	


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5g9 	$ Q	
fQ 2>   v@Y_fE!	d	 W .&vv% > oJ`
^Yj }77M   4DOR
 #[ i /Z	m	

qwUwN 3D
i
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!4> oZ3UNn
8EN :q		N


$=rK( 7 D  ~<-	X/Zg<+  .0

   %RN{A>e 	  H
d@ HX			oXeSh   .}
"       ^-q06[a>X KN
		f ;K	   +CYf;Ejs7h	#	0	lJA# 2    Kx&)m%n&hP,  	]
  =	f		A8 1?#
eD 	
 V -%6L wl		Mv C ;M*    $Mz|	GW	c :]Be/	e 1  >$
 k	LK	   9\=KJ  +  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                #                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  	                                                                                                                                          
                                                                                                                                                                                                                                                   
                                                                                          	                     %   !            	                                                	         	                                                       !                      b   a         "         6   J                                                                                                                                                                           #                                                                                                                                                                                                                                                                                                  	                                                        
                                                                                               
                                	       % !    	                	   	                   !        b a   "   6 J   f  g  g  g  k!  n  t3L&>z#)0[!	

B} ` JO744   -)<JlH	%	<	3L4O#47 	
\
K
\

\
!	5)
  mH&m "i'G
E -p	0u p   2W]_l	  H&H>(	3
<-mg  e0{v	~~e
_7dMV~|{  u   G$1  }   ,2W] s|_H
3NH_    $\-Cz#EKa#	fMA /WZM H _ VSHYg	c vV~
M/@9=T.	uM C#E#	Al 
 N,7|2 CE#	A	Cj7pi 
'iO [(vl u 
W9l u ~

p \2I

dpG~B!  CEod#	AJ u 3HNzf^ 	&:		z

p N,| 8}eV Kx)m-%  J

!dB)h=Q"c	]	/   (#J 8p-	H' ]-q6a> -;E  (#JpB)h=c	]	Q"8 &
%  BG)X]h:=DGQ"c		+	]	k@V  K]x-q)6am->% @
fR la\
>u$2  &hPK> RX
> 
NKrK'2{fSGBe/	 SGPk{F	b
q
3Q4fGt <PT|~MIsi
a] *cTM	Q.}1Ou>j'*/ /	3FQfM	.OGPk{b
q
4G  GX]:DG	+	k@2'y
w][.

J	 FTDZ\Ft@G	$	1	TB  +CYf;Ejs7h	#	0	lJA    GU!E]GSqfq%	+	%Cd w	?1X|D	o	

2p:<LP  6E[Fa		Si /Z	m	

qwUwN `   O%-8}eH
  /  I!'9I-	jH(?     ^-q06[a>X x

b-<
  / A   I{+;!',9?I <1X|mu	-	;	D	o		

2;jNWp:<H(?LP vx;=?dox0y8	>	@	g						

!
T
x






bLhj]_jl8C\ T~:BU`Y8`|+- -4<s 
1  ntW@<|+)$E			4
Q


=6&.n
h   $Mz|	GW	c 6MPh'QMFm  "&G			O




>`lGDg8e1k7x8ss   
7y?1X|FD	o		

2",1^pu:<nLP: ^ v |   >O\V	GYa   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="16"><Feature recentID="0" name="ORF2" directionality="1" translationMW="16700.57" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4956-5378" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="ORF2"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIEIFKFSKKHKKINKKLIDLTKVKQVVTTGYSFIVVYQRGRKIELSYESSYWHAKKDLDNSNSDVGITYSYIKKDKRGPCHKDNYLRDSKGRLLKDWGKIEELHEKYPKIGKIWYISREETDKLLKDFTYKNGNLIMV*"/></Q></Feature><Feature recentID="1" name="repA" directionality="1" translationMW="40263.13" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3934-4953" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="repA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKEVKNVNAGKEIAMSNTLVAGKYSLTKEEQNLIFLVASMVKREDKEFHRYKISLSDLEKATGVKHNRVRLKQLMHSIMSKPVWLNKEQTKIANWFAYIEADPKSSALICEFHWSLMPHMIQLQEYFTKAERQLLFSFKSKYSSRLYLLLKSKLGEQAGYANIVDCVLYVDDMINDFDLPKSYSNRYSNFKNKFLLPALEEINQLSDIFVTYDDKDHHRKHGRKITNIKFTVSKVAETEEEFKQKLLDTKNKEDYIPIEASERLREVVLSDELDLDLYYVRSIFRHHHLHDIERVCDDTWRNWDNKKIKDRKRVFIANIKKLEKKPEENHKLPFGFEEV*"/></Q></Feature><Feature recentID="2" name="ori" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3668-3847" color="#a6acb3" type="standard"/><Q name="gene"><V text="ori"/></Q><Q name="product"><V text="pFNL10 origin of replication"/></Q></Feature><Feature recentID="3" name="'''mcs'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3500-3553" color="#a6acb3" type="standard"/><Q name="gene"><V text="'''mcs'"/></Q><Q name="product"><V text="ks polylinker"/></Q></Feature><Feature recentID="4" name="tnpR" directionality="1" translationMW="20362.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2947-3498" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="tnpR"/></Q><Q name="product"><V text="100 pct identical to gp:ECOTNPA_2[gamma delta resolvase(Tn1000)]"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MRLFGYARVSTSQQSLDIQVRALKDAGVKANRIFTDKASGSSSDRKGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDGISTDGEMGKMVVTILSAVAQAERQRILERTNEGRQEAMAKGVVFGRKRKIDRDAVLNMWQQGLGASHISKTMNIARSTVYKVINESN*"/></Q></Feature><Feature recentID="5" name="''mcs'''" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2923-2941" color="#a6acb3" type="standard"/><Q name="gene"><V text="''mcs'''"/></Q><Q name="product"><V text="ks polylinker"/></Q></Feature><Feature recentID="6" name="blaB'" directionality="1" translationMW="1259.64" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2933" maxFusedRunOn="2933"><Segment range="2886-2918" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="blaB'"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MRLLVTTLSLI"/></Q></Feature><Feature recentID="7" name="ORF-3'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2358-2450" color="#993366" type="standard"/><Q name="gene"><V text="ORF-3'"/></Q><Q name="product"><V text="Hypothetical Protein"/></Q></Feature><Feature recentID="8" name="(null)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1521-1921" color="#993366" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="ColE1 direction"/></Q></Feature><Feature recentID="9" name="oriE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1521-1921" color="#a6acb3" type="standard"/><Q name="gene"><V text="oriE"/></Q><Q name="product"><V text="oriE"/></Q></Feature><Feature recentID="10" name="(null)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1265-1270" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="11" name="(null)" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1241-1246" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="12" name="PgroESL" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1235-1270" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="13" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1235-1235" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="14" name="hyg" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-1145" color="#993366" type="standard"/><Q name="gene"><V text="hyg"/></Q><Q name="product"><V text="hygromycin phosphotransferase"/></Q></Feature><Feature recentID="15" name="rrnABt1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="25-95" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnABt1"/></Q><Q name="product"><V text="rrnAB transcriptional terminator"/></Q></Feature></Features>
  	<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL405  Hyg_PCR_F" sequence="catcgatttaactgcaggtccgct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-02-01T21:39:40Z"><BindingSite location="122-145" boundStrand="0" annealedBases="catcgatttaactgcaggtccgct" meltingTemperature="61"><Component hybridizedRange="122-145" bases="catcgatttaactgcaggtccgct"/></BindingSite><BindingSite simplified="1" location="122-145" boundStrand="0" annealedBases="catcgatttaactgcaggtccgct" meltingTemperature="61"><Component hybridizedRange="122-145" bases="catcgatttaactgcaggtccgct"/></BindingSite></Primer><Primer recentID="1" name="KROL406  Hyg_PCR_R" sequence="caggaattctgggagccgctgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-02-01T21:40:45Z"><BindingSite location="1051-1072" boundStrand="1" annealedBases="caggaattctgggagccgctgg" meltingTemperature="63"><Component hybridizedRange="1051-1072" bases="caggaattctgggagccgctgg"/></BindingSite><BindingSite simplified="1" location="1051-1072" boundStrand="1" annealedBases="caggaattctgggagccgctgg" meltingTemperature="63"><Component hybridizedRange="1051-1072" bases="caggaattctgggagccgctgg"/></BindingSite></Primer><Primer recentID="2" name="KROL409  Hyg_PCR_F2" sequence="tgcaccaggctgtagcgggagt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-02-17T21:36:37Z"><BindingSite location="372-393" boundStrand="0" annealedBases="tgcaccaggctgtagcgggagt" meltingTemperature="67"><Component hybridizedRange="372-393" bases="tgcaccaggctgtagcgggagt"/></BindingSite><BindingSite simplified="1" location="372-393" boundStrand="0" annealedBases="tgcaccaggctgtagcgggagt" meltingTemperature="67"><Component hybridizedRange="372-393" bases="tgcaccaggctgtagcgggagt"/></BindingSite></Primer><Primer recentID="3" name="KROL410  Hyg_PCR_R2" sequence="agagcctcgcgtcggagtcgga" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2021-02-17T21:38:26Z"><BindingSite location="894-915" boundStrand="1" annealedBases="agagcctcgcgtcggagtcgga" meltingTemperature="67"><Component hybridizedRange="894-915" bases="agagcctcgcgtcggagtcgga"/></BindingSite><BindingSite simplified="1" location="894-915" boundStrand="1" annealedBases="agagcctcgcgtcggagtcgga" meltingTemperature="67"><Component hybridizedRange="894-915" bases="agagcctcgcgtcggagtcgga"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>304688ef-354b-43ef-9c86-126a27925187</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Ligation of Fragment 2 into Fragment 2.</Description>
<Created UTC="4:0:0">2016.3.14</Created>
<LastModified UTC="21:38:26">2021.2.17</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
  Y    K         Unsaved Enzyme Set                                                                                           d     CHO               E              Y? P                                                                                                                                                              <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="EcoRI"/></CustomEnzymeSets>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
