	   SnapGene      'tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaatcgatcaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgatctatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaacgatccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctctagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggctgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcagatatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgaacattcggatccccgggtaccaactatgtaaactcattaaacataaaatattcagatagtattgaagttttgtacaactggaattcacttgagtcaacaactattaccataaaatttgaggatggtagtaatgagcaaataagctatcaaggaaaatggtctatattaaaagcaattaaagacgcaaattgtgatcaaaacaatatttgtacctggataatcaagcacaaaggtaaaaaatatcctttaagcttcaaaatagaatcaaaattcttacaagtattaggatggcaaaaacaaggagaagtcaatgtacaagcggccgcatatactgatattgaaatgaatagattaggtaaataagaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacccgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac  2O               	          	                                          %                                                                $                                                          %                                                                      '   `   7   !      "   $   "                                 #            %                                 #            
                                       !            "   
                     	         ,                                    ,            !         .      M   !                                                                                                                 $               .                     	                                                                      "                                              7ZD	' \[E_f( 8	dd nq

><5kx=	 \Ab/)   H/F`2f$pg=` @ ]x0
b
D:>9e	 zB Z p  9	l90Gs1  9 O    h@	}iP{   j).%	JSe!1 9d3'q		;
ef W or?z-6tl.>t4S ,  _
	

K(~ ~ 'A+P/=N Wn s <JqT[r
Ty. M 7]oB3c,/ j5ic0K* 	 c  	B7s	+uH I QGo2eMrw k    07Gf	%^	h-a%  Mragk*U

	4GqLeP$oA{7 :3"T~>FY  I \	z

T  &`c2AipF		;:Ywf FOkQ
5?@H$;L A ^l	Hw;

1
c
 K  #+y]zD0Eo >P/D{	"  c	=W>  "%
k
a~C D	
XX} n q  F:xI:2 9	

m1Jy i	Hw [ 	^0tl T   	~j  : DK 			^	mQ_! P   iA	
)(8| Xd	,5&  /Y	/	

K"c  WLk&	k			rf'B$   *`u6		,
M


wTn3S0p2V e I	P	'6jx 4Z-fi=g eJ g+  :f	bV	r		<
 Z  (MV% X  Xp	o	b{n.7Ev,%/ry K N3ux5
@2mu5 X I%\4	6T  
s	
Y`vu[#?`n - 0 F  `]c	h
 ;})ak   S;		5
]t Mfp

Lnw2 I	T    ,QJ>x!Xm]o ]  'P/| (m(02	v		Od{OI   ABkl8DAQb%dp !$),r[ w/% & 3  KS|		7q]6?HO t uS;U\
		s

gzz =		AhU
 [m	=c4j ? w@a
C9a.d
A N 28g$		8O0 V ^IpZ'sSV- R H b  h*t1L+.   69j)eT
y

dX "kGj	XG#JK  b!	$
j
 m E
8	
  C			+'l d   _t			

v2A/ 
 a,YI* J  2 Hn	mu3+x / E   L	:|`s  l7I&C.N{lc 2  RR	p\f>	  ZvfH?#		`
&8`Ri    !8Fig		x
WJ l `		
@.)   1kK6_t
Bz*b'  u>h5"			_
w


7sQ?I`   Nstst#0189					V
^




!56HIJrfK%9EZ[\]bcdep\7 u$2{l:X	W



"KMg&Ffq]vBS|!   Oy,b&t+R=	 
@L:^8 A%	MmUo#C" v3#;U)			



NhwGT$ 6|4Yt
;gr?^Z}" {;I}X


#L'Ga JU\c&,AS
	M  <   -;]2	AD;_M x Pz'u
#>	7
{
_9P   
U9 BhN>	
<g hO
a BRqVpD R G	T	b		;%$e G'&9d3njiCZx kR


6%Hj ?w4$rGV	\



SIZxUG# V<Pl	

+@Ay!#4< b*		BOi6M 
ICZu2
*>~: B mx$!
<TL%  _75O<en	
@
d


{!s*hs_ .X}


KR@[  # M	F-MpH p<H~>	Y


NYpb~  ,z^


1n(  $|!NT{JE`$2 | 5]0'-o

  KEB|N UdT
 ?QV		u!# = `  
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[Z; OV e		Bi \g }Z	
Z[   )  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                %                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
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               !                                    
                           	                  	                                    "                              `   Q         $         !   E                                                                                                                                                                           %                                                                                                                                                                                                                                                                                                                                                                       
                                                     '                                                                                
     !            
         	      	            "          ` Q   $   ! E   f(  f  g  g(  i  mD  r>"u \[\"
a1 $U7OF	)		2	7Y%.n6?Om6ytQ
~ IX, v9kvA y0Z	Z  rw	-{3fq>]Y r	ulirV^ ru>Ar  	E&n}\$w-n-e4%f	=|Bf $Q		7}dwDvu"x	t63 .  	9{ >YdnJk(x	
,6	J g  f"\cK!,Ik Az$w Hl
[lH
 l 	E&[.dc	+4%ixD #zDXG*{P]	V	- 0  >kxX]o<'q
Tx >~	l0~pO8'- 0  k>xX]oLF^	;f| -
[- 0  !p)r >kxX,/]or >~XG*{P]Ln&c2iYw) 6 ($8
: W:9e M _1B+cM ' ,	f	`B+cD	( 5 tT>P	"X
ZXbv%	 :~%
+~HOYh! ;A
!,z fCG%h:  k			r!  7f	{>y	2)}C	 +H%
~	:~!p)r ,/r( 5 Dt	T RD!bJ	Q	
V0U	MWWt

Xa "$ "L*s
WbRl40G[X  Xp	o	b{n.7Ev,%/ryXd	,5& l7I&C.N{lc 
s	
Y`vu[#?`n $(0 #+y]zD0Eos <JqT[r
Ty.  %?-xgFgS	
d2 s ~   '<AJRlRq+PT/7I[	r
&p=N\Tf >CWy.N{.lnc	Q gFj>aNU8gq=QS	a			[




*ARhBx "$Y35diFUdT
 Heo\b:	4
g

j6|4Yt
;gr?^Z}T<		
E
!j{MhM 7]oB3c,/X}*9yr'	
9=CN   U28g ld,7IT$			
&08
C.N{Ol0c5[mn
.>_I   <       (        (.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#љ:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk}y^WeY&*ׁkz^iUAIa5e9X.fMiQZ!]f~[SuBUY:d[UI5WPE-bu6ghVFZmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ      '   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  +r<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="hypothetical protein" directionality="1" translationMW="11507.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="995" maxRunOn="4938" maxFusedRunOn="4938"><Segment range="4612-4908" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="FTL_0125" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4612-4908" color="#00e600" type="standard"/></Feature><Feature recentID="27" name="hypothetical protein" directionality="1" translationMW="312.39" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4927" maxFusedRunOn="4927"><Segment range="4901-4908" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="FTL_0124" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4901-4908" color="#00e600" type="standard"/></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4612-4908" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5378-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5003-5022" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4981-5006" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="21" name="VSVG peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4920-4955" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="VSVG"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="FTL_1606" directionality="1" translationMW="89.09" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="169" maxRunOn="4955" maxFusedRunOn="4955"><Segment range="4909-4915" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256883" predef="GI"/><V text="3951956" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="FTL_1606"/></Q><Q name="locus_tag"><V text="FTL_1606"/></Q><Q name="note"><V text="/dnas_title=stringent starvation protein A fragment, regulator of transcription"/></Q><Q name="product"><V text="stringent starvation protein A, regulator of transcription"/></Q><Q name="protein_id"><V text="YP_514245.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="groES" directionality="1" translateFirstCodonAsMet="1" translationMW="357.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4621" maxFusedRunOn="4621"><Segment range="4592-4601" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256984" predef="GI"/><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms&#10;homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/><V text="/dnas_title=co-chaperonin GroES"/></Q><Q name="product"><V text="co-chaperonin GroES"/></Q><Q name="protein_id"><V text="YP_514346.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-4601" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q></Feature><Feature recentID="17" name="P051 groESpromoR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4574-4601" color="#a6acb3" type="standard"/><Q name="label"><V text="P051 groESpromoR"/></Q><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="16" name="P052 groESpromoF" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4381" color="#a6acb3" type="standard"/><Q name="label"><V text="P052 groESpromoF"/></Q><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="15" name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter"/></Q></Feature><Feature recentID="14" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4295-4314" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="13" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4271-4294" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3395" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="10" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="9" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="8" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="7" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="6" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="4" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="3" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="2" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="1" name="/dnas_title=pKL13 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="note"><V text="/dnas_title=pKL13 Verified"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="sub_species"><V text="holarctica"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5672" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="KROL219 pKR40_F" sequence="atatggtaccaactatgtaaactcattaaacataaaatatt" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-01T22:58:27Z"><BindingSite location="4605-4641" boundStrand="0" annealedBases="ggtaccaactatgtaaactcattaaacataaaatatt" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="4605-4641" bases="ggtaccaactatgtaaactcattaaacataaaatatt"/></BindingSite><BindingSite simplified="1" location="4605-4641" boundStrand="0" annealedBases="ggtaccaactatgtaaactcattaaacataaaatatt" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="4605-4641" bases="ggtaccaactatgtaaactcattaaacataaaatatt"/></BindingSite></Primer><Primer recentID="1" name="KROL220 pKR40_R" sequence="atatgcggccGCttgtacattgacttctccttgtttttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-01T23:30:21Z"><BindingSite location="4881-4921" boundStrand="1" annealedBases="atatgcggccGCttgtacattgacttctccttgtttttg" meltingTemperature="64"><Component hybridizedRange="4909-4921" bases="atatgcggccGCt"/><Component hybridizedRange="4881-4906" bases="tgtacattgacttctccttgtttttg"/></BindingSite><BindingSite simplified="1" location="4881-4921" boundStrand="1" annealedBases="atatgcggccGCttgtacattgacttctccttgtttttg" meltingTemperature="64"><Component hybridizedRange="4909-4921" bases="atatgcggccGCt"/><Component hybridizedRange="4881-4906" bases="tgtacattgacttctccttgtttttg"/></BindingSite></Primer><Primer recentID="0" name="P035 pEX18integrationVSVG" sequence="tttacctaatctattcatttcaatatcag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4923-4951" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="52"><Component hybridizedRange="4923-4951" bases="tttacctaatctattcatttcaatatcag"/></BindingSite><BindingSite simplified="1" location="4923-4951" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="52"><Component hybridizedRange="4923-4951" bases="tttacctaatctattcatttcaatatcag"/></BindingSite></Primer></Primers>
  p<Notes>
<UUID>947206a8-50b3-4290-b0d9-81a52f903fc9</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="16:39:3">2019.9.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  0                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#љ:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk}EY;*dYdU6ZF:9bJXLQ鳡heoImZ]ѴmcmbGQRXSx;Dn:ѢbSEaf ֦eUGDkKUSeP8!-^D͙%e[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%         -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %C<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5583-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5208-5227" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5186-5211" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="21" name="VSVG peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5125-5160" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="VSVG"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="FTL_1606" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5160" maxFusedRunOn="5160"><Segment range="4612-5120" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256883" predef="GI"/><V text="3951956" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="FTL_1606"/></Q><Q name="locus_tag"><V text="FTL_1606"/></Q><Q name="note"><V text="/dnas_title=stringent starvation protein A fragment, regulator of transcription"/></Q><Q name="product"><V text="stringent starvation protein A, regulator of transcription"/></Q><Q name="protein_id"><V text="YP_514245.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="groES" directionality="1" translateFirstCodonAsMet="1" translationMW="357.33" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4621" maxFusedRunOn="4621"><Segment range="4592-4601" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256984" predef="GI"/><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms&#10;homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/><V text="/dnas_title=co-chaperonin GroES"/></Q><Q name="product"><V text="co-chaperonin GroES"/></Q><Q name="protein_id"><V text="YP_514346.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-4601" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q></Feature><Feature recentID="17" name="P051 groESpromoR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4574-4601" color="#a6acb3" type="standard"/><Q name="label"><V text="P051 groESpromoR"/></Q><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="16" name="P052 groESpromoF" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4381" color="#a6acb3" type="standard"/><Q name="label"><V text="P052 groESpromoF"/></Q><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="15" name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter"/></Q></Feature><Feature recentID="14" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4295-4314" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="13" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4271-4294" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3395" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="10" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="9" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="8" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="7" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="6" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="4" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="3" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="2" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="1" name="/dnas_title=pKL13 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="note"><V text="/dnas_title=pKL13 Verified"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="sub_species"><V text="holarctica"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5877" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P035 pEX18integrationVSVG" sequence="tttacctaatctattcatttcaatatcag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5128-5156" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="51"><Component hybridizedRange="5128-5156" bases="tttacctaatctattcatttcaatatcag"/></BindingSite><BindingSite simplified="1" location="5128-5156" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="51"><Component hybridizedRange="5128-5156" bases="tttacctaatctattcatttcaatatcag"/></BindingSite></Primer></Primers>
  q<Notes>
<UUID>947206a8-50b3-4290-b0d9-81a52f903fc9</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="16:38:57">2019.9.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *<?xml version="1.0"?><HistoryTree><Node name="pKR48 pEX3_FTL_0125-V.dna" type="DNA" seqLen="5670" ID="2" circular="1" operation="remove"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4908..5615" colors="blue-black"/><ColorRange range="4200..4907" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4908..5615" colors="blue-black"/><ColorRange range="4200..4907" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4908" val2="4909"/><Node name="pKR48 pEX3_FTL_0125-V.dna" type="DNA" seqLen="5672" ID="1" circular="1" resurrectable="1" operation="replace"><Features><Feature name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature name="FTL_1606" directionality="1" translationMW="89.09" type="CDS" allowSegmentOverlaps="0"><Segment range="4909-4915" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256883" predef="GI"/><V text="3951956" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="FTL_1606"/></Q><Q name="locus_tag"><V text="FTL_1606"/></Q><Q name="note"><V text="/dnas_title=stringent starvation protein A fragment, regulator of transcription"/></Q><Q name="product"><V text="stringent starvation protein A, regulator of transcription"/></Q><Q name="protein_id"><V text="YP_514245.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="A"/></Q></Feature><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4295-4314" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4271-4294" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3371-3395" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4981-5006" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="VSVG peptide" type="sig_peptide" allowSegmentOverlaps="0"><Segment range="4920-4955" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="VSVG"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4612-4908" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5378-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4910..5618" colors="blue-black"/><ColorRange range="4199..4907" colors="black-red"/><ColorRange range="4908..4909" colors="white"/></TopStrand><BottomStrand><ColorRange range="4910..5618" colors="blue-black"/><ColorRange range="4199..4907" colors="black-red"/><ColorRange range="4908..4909" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4611..4907" colors="red"/></TopStrand><BottomStrand><ColorRange range="4611..4907" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="4611" val2="5112"/><Node name="pKR48 pEX3_FTL_0125-V.dna" type="DNA" seqLen="5877" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4295-4314" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4271-4294" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3371-3395" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5186-5211" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="VSVG peptide" type="sig_peptide" allowSegmentOverlaps="0"><Segment range="5125-5160" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="VSVG"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="FTL_1606" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4612-5120" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256883" predef="GI"/><V text="3951956" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="FTL_1606"/></Q><Q name="locus_tag"><V text="FTL_1606"/></Q><Q name="note"><V text="/dnas_title=stringent starvation protein A fragment, regulator of transcription"/></Q><Q name="product"><V text="stringent starvation protein A, regulator of transcription"/></Q><Q name="protein_id"><V text="YP_514245.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KKIAPNGVFPILMEKDYSINNRKALLIYIDERFPAPSLLPNVVNERIKIRLSLDKIDNEWYPVLDQIRKHRSDQKMLESMFKDLKESLLAMEKAFTGSEFFISSGFTLADCYIAALIICLEAEGFIIDDEYGAIYEYKKRLFARDSVKKANIKGGAGESLLKTLRTHRA"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5583-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4611..5112" colors="white"/></TopStrand><BottomStrand><ColorRange range="4611..5112" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,b<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="hypothetical protein" directionality="1" translationMW="11507.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="995" maxRunOn="4953" maxFusedRunOn="4953"><Segment range="4612-4908" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="NYVNSLNIKYSDSIEVLYNWNSLESTTITIKFEDGSNEQISYQGKWSILKAIKDANCDQNNICTWIIKHKGKKYPLSFKIESKFLQVLGWQKQGEVNVQ"/></Q></Feature><Feature recentID="28" name="FTL_0125" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4612-4908" color="#00e600" type="standard"/></Feature><Feature recentID="27" name="hypothetical protein" directionality="1" translationMW="312.39" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4936" maxFusedRunOn="4936"><Segment range="4901-4908" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MY"/></Q></Feature><Feature recentID="26" name="FTL_0124" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4901-4908" color="#00e600" type="standard"/></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4612-4908" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5376-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5001-5020" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4979-5004" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="21" name="VSVG peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4918-4953" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="VSVG"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="FTL_1606" directionality="1" translationMW="89.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="169" maxRunOn="4953" maxFusedRunOn="4953"><Segment range="4909-4913" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256883" predef="GI"/><V text="3951956" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="FTL_1606"/></Q><Q name="locus_tag"><V text="FTL_1606"/></Q><Q name="note"><V text="/dnas_title=stringent starvation protein A fragment, regulator of transcription"/></Q><Q name="product"><V text="stringent starvation protein A, regulator of transcription"/></Q><Q name="protein_id"><V text="YP_514245.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="A"/></Q></Feature><Feature recentID="19" name="groES" directionality="1" translateFirstCodonAsMet="1" translationMW="357.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4621" maxFusedRunOn="4621"><Segment range="4592-4601" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256984" predef="GI"/><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms&#10;homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/><V text="/dnas_title=co-chaperonin GroES"/></Q><Q name="product"><V text="co-chaperonin GroES"/></Q><Q name="protein_id"><V text="YP_514346.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HSD"/></Q></Feature><Feature recentID="18" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4592-4601" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3951567" predef="GeneID"/></Q><Q name="gene"><V text="groES"/></Q><Q name="label"><V text="groES"/></Q><Q name="locus_tag"><V text="FTL_1715"/></Q></Feature><Feature recentID="17" name="P051 groESpromoR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4574-4601" color="#a6acb3" type="standard"/><Q name="label"><V text="P051 groESpromoR"/></Q><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="16" name="P052 groESpromoF" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4381" color="#a6acb3" type="standard"/><Q name="label"><V text="P052 groESpromoF"/></Q><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="15" name="groES promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="note"><V text="groES promoter"/></Q></Feature><Feature recentID="14" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4295-4314" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="13" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4271-4294" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3395" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="10" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="9" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="8" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="7" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="6" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="4" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="3" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="2" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="1" name="/dnas_title=pKL13 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4353-4601" color="#ffffff" type="standard"/><Q name="db_xref"><V text="119857" predef="taxon"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="note"><V text="/dnas_title=pKL13 Verified"/></Q><Q name="organism"><V text="Francisella tularensis subsp. holarctica"/></Q><Q name="strain"><V text="LVS"/></Q><Q name="sub_species"><V text="holarctica"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5670" color="#ffffff" type="standard"/><Q name="mol_type"><V predef="other DNA"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL240 katg-ck-1" sequence="gattacgaattcttatttacctaatctat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-05T16:40:40Z"><BindingSite location="4936-4964" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="4936-4964" bases="gattacgaattcttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="4936-4964" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="4936-4964" bases="gattacgaattcttatttacctaatctat"/></BindingSite></Primer><Primer recentID="2" name="KROL219 pKR40_F" sequence="atatggtaccaactatgtaaactcattaaacataaaatatt" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-01T22:58:27Z"><BindingSite location="4605-4641" boundStrand="0" annealedBases="ggtaccaactatgtaaactcattaaacataaaatatt" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="4605-4641" bases="ggtaccaactatgtaaactcattaaacataaaatatt"/></BindingSite><BindingSite simplified="1" location="4605-4641" boundStrand="0" annealedBases="ggtaccaactatgtaaactcattaaacataaaatatt" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="4605-4641" bases="ggtaccaactatgtaaactcattaaacataaaatatt"/></BindingSite></Primer><Primer recentID="1" name="KROL220 pKR40_R" sequence="atatgcggccGCttgtacattgacttctccttgtttttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-01T23:30:21Z"><BindingSite location="4881-4919" boundStrand="1" annealedBases="atatgcggccGCttgtacattgacttctccttgtttttg" meltingTemperature="67"><Component hybridizedRange="4881-4919" bases="atatgcggccGCttgtacattgacttctccttgtttttg"/></BindingSite><BindingSite simplified="1" location="4881-4919" boundStrand="1" annealedBases="atatgcggccGCttgtacattgacttctccttgtttttg" meltingTemperature="67"><Component hybridizedRange="4881-4919" bases="atatgcggccGCttgtacattgacttctccttgtttttg"/></BindingSite></Primer><Primer recentID="0" name="P035 pEX18integrationVSVG" sequence="tttacctaatctattcatttcaatatcag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4921-4949" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="52"><Component hybridizedRange="4921-4949" bases="tttacctaatctattcatttcaatatcag"/></BindingSite><BindingSite simplified="1" location="4921-4949" boundStrand="1" annealedBases="tttacctaatctattcatttcaatatcag" meltingTemperature="52"><Component hybridizedRange="4921-4949" bases="tttacctaatctattcatttcaatatcag"/></BindingSite></Primer></Primers>
  q<Notes>
<UUID>947206a8-50b3-4290-b0d9-81a52f903fc9</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="16:40:41">2019.9.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO  d              E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
