	   SnapGene      tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaatcgatcaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgatctatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaacgatccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctctagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggctgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagaggatccccgggtacCgagcaatgttataaagatggtgtaaatcaagattatgagaaagttttggagtcttgtaaaccatacttgaaaactgatgcacgtgccactggtcttctagcagaggcaaatattcagcttgattcaagtgataaattagctctagaggatgcgttatgggcagtaaatttttatcaaaaaaatggtgctccaaaagatccagaaggtgctagattgtattcttatttagtatatctgattggtgagttatatttctttgattctgacaatattaaggtagatcaaaaaaaaggtatagaatatattataaaatcagctaatttgggctatgatattgcacaaaatcagttaggaaacctctacgtcagagcaggaaaagttcctggcccaaattttgctaaggcttataaatggtataagttagctattgctaatggtagtctagatgcacgtaatgcttacctgatgaataatgaaagaagctttatagaaaactatccatactgtatatcgattggtagagcgctaattggcgatacatatttaaatggtttggcaggtttaccaaagagccctgattttgcaattcaatggtatcaaaaagcatacgagctagatcatatatctcctgtagaaactggcctagcaagagcatatatggcaaaaggagataaaaagcttgcttataagtatgcgcgtgaagcaatacaacagccatatgccccagcatttgttgtaattgcagatttaactgataacaaagtggctaaatatgcatatctagcggaagctgtcgaattgtataaaaatccagcacttaaattctggagtcagtttaatgagtattgtatgccagatatttctgataatggtctgaagcaagcgcagcgaaatcttgctaaaattaaattatcaaagcaagagttagaattagctaccaaagagcagcaaagtttgagaaatagctggcaacaaaatgcggtgacttctaaaGCggccgcatatactgatattgaaatgaatagattaggtaaataagaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacccgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac  ,  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                            ,                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
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                                                       
                                                  *                                                               	        	    
     #    	                 	         
            " !          k e 	  V X %    Q   5  6  7  7  9  =  Du \[\O"
a1 $U7OF			2	7Y%.n6?OmDtQ
}Q
}Q
}}} IX vkv3 y0FZ	Z  ArQB	{3I$ er	ulO4rqXV^ ru	{ArN  	ne}'U$wn0Of	=|f $Q		7}dwDv@"x	t463a N.  	9{ $dnk(x	
,6ME g  f"\cK!,0Mp AE$w Hl&lH l 	![./c	+ixD #zDi hp YZ5	}X`
XG*{(	V	- 0  >kxX(:<'q
Tx >~	l0~pO8'- 0  k>xX(:LF^	;%| -
[- 0  !p)r >kxX(:=N >~XG*{(Ln&c2iYwa) 6 ($8
m W:9 M _1B+cM ' ,	f	`aB+cD	( 5 tl>P	X
%Xbv	 :~%
+FQnOYh ;A
s~E fCmG%hB/:  k			r  7f	{>y	2)}r	 +FQ%
	:~n!p)r =N( 5 Dt	l R!bJ	Q	
V0U	MWWt

Xa  "L*s
WbRl4Q&ObX  Xp	o	b{n.7Ev%=DYXd	, l7I&CPx*7L. 
s	
Y`vu[ 
+9| $(vz" #+y]zD0Eo-}s <JqT[r
Ty  %?-xmVgFgS	
/2 s ~   '<AJRlRq+PT/7I[	r
&p=N\Tf >CWyPZPx*7k9L.Q gFj>aNU8gq=QS	a			[




*ARhBx7S;BG]	0r7f /4etUdT
f Heo\b:	4
g

jce[   %?-P]VLGX		}			8rGx&*W{S@j?m(@KV] * 7 >   %1?-)Rd%Y9
<~"Hxb+r-|UnBNmr~)V6|4Yt
T%^2J%HYT<		
E
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%5GYN   U28g ld,7IT$			
&08
Cf(8PyCx*7L.\5[mn
.>_/  =R     y  ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>          @  C  :   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4673" color="#00e600" type="standard"/></Feature><Feature recentID="25" name="hypothetical protein" directionality="1" translationMW="12027.40" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="4718" maxFusedRunOn="4718"><Segment range="4375-4673" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4668-4673" color="#a6acb3" type="standard"/></Feature><Feature recentID="23" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" 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range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="19" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin 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recentID="10" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="9" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="8" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="7" name="P001 FTL_1743fragR" directionality="2" 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consecutiveTranslationNumbering="1"><Segment range="4690-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="3" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4739-4764" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="2" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4761-4780" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="1" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4899-4922" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5135-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL240" sequence="gattacgaattcttatttacctaatctat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-23T19:43:04Z"><BindingSite location="4701-4729" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="4701-4729" bases="gattacgaattcttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="4701-4729" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="4701-4729" bases="gattacgaattcttatttacctaatctat"/></BindingSite></Primer><Primer recentID="1" name="KROL213" sequence="ctagaggatccccgggtacCaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:11:36Z"><BindingSite location="4354-4377" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="4354-4377" bases="ctagaggatccccgggtacCaagg"/></BindingSite><BindingSite simplified="1" location="4354-4377" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="4354-4377" bases="ctagaggatccccgggtacCaagg"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="19:43:10">2019.7.23</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  +                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	ZaB%*-QI)%zUW%U&i`&멹-:Q#:WVZAQV+F}4'QTKNUUc!EfᩲQ[k[TQW;-j<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="27" name="/dnas_title=pKL02 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5635" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL02 Verified"/></Q></Feature><Feature recentID="26" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="25" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="24" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="23" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="note"><V text="P261 groESpCheck1"/></Q><Q name="label"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="22" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="note"><V text="P049 groES promoter F"/></Q><Q name="label"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="21" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="20" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q><Q name="label"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="note"><V text="P442 pEX_kanR_F"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="note"><V text="P443 pEX_kanR_R"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="17" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="16" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="note"><V text="P441 pEX_ckR"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="15" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q><Q name="label"><V text="sacB"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q><Q name="label"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="10" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q><Q name="label"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="9" name="FTL_1743 fragment" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4873" color="#993366" type="standard"/><Q name="note"><V text="FTL_1743 fragment"/></Q><Q name="label"><V text="FTL_1743 fragment"/></Q></Feature><Feature recentID="8" name="P002 FTL_1743fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4401" color="#a020f0" type="standard"/><Q name="note"><V text="P002 FTL_1743fragF"/></Q><Q name="label"><V text="P002 FTL_1743fragF"/></Q></Feature><Feature recentID="7" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4848-4918" color="#a020f0" type="standard"/><Q name="note"><V text="P001 FTL_1743fragR"/></Q><Q name="label"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="6" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4883-4918" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="5" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4887-4915" color="#a020f0" type="standard"/><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="4" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4890-4919" color="#a020f0" type="standard"/><Q name="note"><V text="P226 SP_pKL02_F"/></Q><Q name="label"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="3" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4939-4964" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q><Q name="label"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="2" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4961-4980" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q><Q name="label"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="1" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5099-5122" color="#a020f0" type="standard"/><Q name="note"><V text="P227 SP_pKL02_R"/></Q><Q name="label"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5335-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q><Q name="label"><V text="ColE origin"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  q<Notes>
<UUID>ab50ccb3-a294-49b1-80d4-d64540352dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="19:11:25">2019.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        {  A        Af	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<          
  4  =  @  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  ^<?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-309" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="321" maxFusedRunOn="321"><Segment range="11-309" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="304-309" color="#a6acb3" type="standard"/></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="1" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="2" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="3" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>395d1b84-d57b-4633-89b7-4c0e12448ebb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:10:51">2019.4.9</Created>
<LastModified UTC="19:12:24">2019.4.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       (          f0ͪ(8{ejQ8Ύ}Y6uW#ֱӯ9ޢJVZuՎ ك&3gSSf;fa;&:gV;%~=_jVblgISДr6yY9zgڙOj^rD*xjmi8jfkYmQYљeu7끺MZ?e;NZzf9} ܍Ԙ*u^n}v}&                   -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="13-1005" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_1306" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="13-1005" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="hypothetical protein" directionality="2" translationMW="37464.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="23" maxRunOn="1" maxFusedRunOn="1"><Segment range="13-1005" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL250 pkr47_F" sequence="atatGCGGCCGCtttagaagtcaccgcattttgttgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-29T18:25:59Z"><BindingSite location="0-37" boundStrand="0" annealedBases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc" meltingTemperature="70"><Component hybridizedRange="0-37" bases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc"/></BindingSite><BindingSite simplified="1" location="0-37" boundStrand="0" annealedBases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc" meltingTemperature="70"><Component hybridizedRange="0-37" bases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc"/></BindingSite></Primer><Primer recentID="1" name="KROL251" sequence="atatGGTACCgagcaatgttataaagatggtgtaaatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-29T18:25:59Z"><BindingSite location="977-1014" boundStrand="1" annealedBases="atatGGTACCgagcaatgttataaagatggtgtaaatc" meltingTemperature="61"><Component hybridizedRange="977-1014" bases="atatGGTACCgagcaatgttataaagatggtgtaaatc"/></BindingSite><BindingSite simplified="1" location="977-1014" boundStrand="1" annealedBases="atatGGTACCgagcaatgttataaagatggtgtaaatc" meltingTemperature="61"><Component hybridizedRange="977-1014" bases="atatGGTACCgagcaatgttataaagatggtgtaaatc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>051dd41f-1fd8-4040-adb3-7c866c917344</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="18:26:4">2019.7.29</Created>
<LastModified UTC="18:26:4">2019.7.29</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	7zXZ  ִF !   t/E] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!n<'d,Qޟ3_i8{KEE~]|Q+f`3ky[Zi%4%l_*xk"͔"j_tD]#D¥UʠN,;\\ފ*t²~4l)NH Zv$ xZXF;9\}Hl-^36):6	ᦌkj}P>	콵gg~µ蛂cU-OMrRLFb3(MZ	^FAűf$4ib`ў+/X(6^JtW儵!.G^j~ɯP<x>,_zF@DÁ*X+'e@ s~ώ#,s89`QhsU;-lgiޥ/`0yW#hl=oUdgh,l|9HK蔦hGp?1\ũ*uGv19XC;	+CA"mA\~Ïu@\iv&"{"FvTmf?2d]!@12hvvΑ4E|A"+&$<wXx\5aM%WǕuX71Nꓘ5*%mt/P d"dw!Sz,Q5
CGqvvr70oB76<"Z|$re}gq"kmxWHEJ~iut:A8%'QvO5+}HP_0s(M>+9<z*t5Q_cpQ&dyk_CId{tھ>uO9:WrԢ3/ʞ(e/^BP8g
H<dp\/d;eH&C}J,M=^τ܆SqtECJsJ{ |L1-dw7>
ԖWvX%-88&ơP5>3ȩpZڬZJ@/g	պ"xpDWT	eG9:~JD"`}?9_ϪHF͆,FlZv1A;(36[0vڞOK[eCMKu!ԩ1(vZdͤ\nF.Ns.~D׉{Y )	16,kU0PM%689DB\kex̡4~
ǲ&vT't^m_"?3~c9Wnzݤ`\7AƔFx nvmǒOtC~Nσ1T"%ڈ;\UrK̚(Ӟ&Sw+('"MyӇufBmKIX
˰`(kQ$8.^)ؠ?13"HHJJ(}l޺gox_Gg
ZcD[h
m' N}w<7{pfRbyhMP^Ζp@>{'>QaxrO-g!"cݭW=tڭt
۰j	&Cy:0{7a5tx >7l=.* -kb}LDe)001Td7c*p%̮8SQ_T^jj v;gG\CN5yjOkv:'DDwhULܗ
qlk'AIaSՇaGS&/ q0[X>9Ǧ\F\>Oh~c_-ZPȊܲvx)k JK'J~hN\\WKQxA$QE=_P:YlOD}},F	><9b"&B̜YI"rpK-zcpH,<p؃U)9"ɐk;j.bs|:ǣa<Ì5]<1@:x9YjL嶜
$    ?
1  o%g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !<?xml version="1.0"?><Features nextValidID="26"><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5829-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="1" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5593-5616" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="2" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5455-5474" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="3" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5433-5458" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5384-5413" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="5" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5381-5409" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="6" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5377-5412" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="7" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5370-5412" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="8" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="9" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="10" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="14" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="15" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="16" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="19" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="20" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="22" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="23" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="24" name="hypothetical protein" directionality="1" translationMW="37464.58" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="23" maxRunOn="5412" maxFusedRunOn="5412"><Segment range="4375-5367" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EQCYKDGVNQDYEKVLESCKPYLKTDARATGLLAEANIQLDSSDKLALEDALWAVNFYQKNGAPKDPEGARLYSYLVYLIGELYFFDSDNIKVDQKKGIEYIIKSANLGYDIAQNQLGNLYVRAGKVPGPNFAKAYKWYKLAIANGSLDARNAYLMNNERSFIENYPYCISIGRALIGDTYLNGLAGLPKSPDFAIQWYQKAYELDHISPVETGLARAYMAKGDKKLAYKYAREAIQQPYAPAFVVIADLTDNKVAKYAYLAEAVELYKNPALKFWSQFNEYCMPDISDNGLKQAQRNLAKIKLSKQELELATKEQQSLRNSWQQNAVTSK"/></Q></Feature><Feature recentID="25" name="FTL_1306" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-5367" color="#00e600" type="standard"/></Feature></Features>
  &<?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL251" sequence="atatGGTACCgagcaatgttataaagatggtgtaaatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-29T18:25:59Z"><BindingSite location="4368-4401" boundStrand="0" annealedBases="GGTACCgagcaatgttataaagatggtgtaaatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4401" bases="GGTACCgagcaatgttataaagatggtgtaaatc"/></BindingSite><BindingSite simplified="1" location="4368-4401" boundStrand="0" annealedBases="GGTACCgagcaatgttataaagatggtgtaaatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4401" bases="GGTACCgagcaatgttataaagatggtgtaaatc"/></BindingSite></Primer><Primer recentID="1" name="KROL250 pkr47_F" sequence="atatGCGGCCGCtttagaagtcaccgcattttgttgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-29T18:25:59Z"><BindingSite location="3013-3028" boundStrand="0" annealedBases="ccgattttgtgcc" meltingTemperature="29"><Component bases="atatGCGGCCGCtttagaagtca"/><Component hybridizedRange="3013-3015" bases="ccg"/><Component bases="c"/><Component hybridizedRange="3018-3024" bases="attttgt"/><Component bases="t"/><Component hybridizedRange="3026-3028" bases="gcc"/></BindingSite><BindingSite location="5341-5378" boundStrand="1" annealedBases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc" meltingTemperature="70"><Component hybridizedRange="5341-5378" bases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc"/></BindingSite><BindingSite simplified="1" location="3013-3028" boundStrand="0" annealedBases="ccgattttgtgcc" meltingTemperature="29"><Component bases="atatGCGGCCGCtttagaagtca"/><Component hybridizedRange="3013-3015" bases="ccg"/><Component bases="c"/><Component hybridizedRange="3018-3024" bases="attttgt"/><Component bases="t"/><Component hybridizedRange="3026-3028" bases="gcc"/></BindingSite><BindingSite simplified="1" location="5341-5378" boundStrand="1" annealedBases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc" meltingTemperature="70"><Component hybridizedRange="5341-5378" bases="atatGCGGCCGCtttagaagtcaccgcattttgttgcc"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5454-5473" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="5454-5473" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite location="5704-5714" boundStrand="1" annealedBases="gtgagcgata" meltingTemperature="21"><Component bases="tgtggaatt"/><Component hybridizedRange="5709-5714" bases="gtgagc"/><Component bases="g"/><Component hybridizedRange="5704-5707" bases="gata"/></BindingSite><BindingSite simplified="1" location="5454-5473" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="5454-5473" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="5704-5714" boundStrand="1" annealedBases="gtgagcgata" meltingTemperature="21"><Component bases="tgtggaatt"/><Component hybridizedRange="5709-5714" bases="gtgagc"/><Component bases="g"/><Component hybridizedRange="5704-5707" bases="gata"/></BindingSite></Primer><Primer recentID="4" name="KROL240" sequence="gattacgaattcttatttacctaatctat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-23T19:43:04Z"><BindingSite location="5395-5423" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="5395-5423" bases="gattacgaattcttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="5395-5423" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="5395-5423" bases="gattacgaattcttatttacctaatctat"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>9d7f4a7d-5bcf-48d4-8ee3-17b3904222da</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pKR47</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="18:28:12">2019.7.29</Created>
<LastModified UTC="18:28:40">2019.7.29</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                            d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
