	   SnapGene      :tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaatcgatcaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgatctatacccttcaagctttgaaaaataaacttaattattatatatgttatttagctagtttttttaattaaagttaaaatcgagagcttgtttgacaaaaaaacaaaaaaatttcttgaaaattttttttttgactcaatatctagacttgcaagagcttggaactttgagattgttctaagatgcatacaaattcaaaatgcttaaacaaaaataatttaacaaaggagtaagattgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaacgatccagctgtcccaaacgtccaggccttttgcagaagagatatttttaattgtggacgaatcgaactcaggaacttgatatttttcatttttttgctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgttttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagtatacactttgccctttacacattttaggtcttgcctgctttatcagtaacaaacccgcgcgatttacttttcgacctcattctattagactctcgtttggattgcaactggtctattttcctcttttgtttgatagaaaatcataaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttcattctctgtattttttatagtttctgttgcatgggcataaagttgcctttttaatcacaattcagaaaatatcataatatctcatttcactaaataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctctagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggctgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcaggaaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaaaataaacaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagaggatccccgggtacCttaaaagaaaatggtattgaatgtaagctttttgataaagcatcggataatattggtaacgatcaagtacacagtactgtcaaagctatcgaagcaggtgaagttgacttattggttaatacatctataagacaagaactaaaaataagtttagcattaagaagagctgctattatgagtagagtttcttatgtaacaactattggtgcttttgaggcttttgttatagctgttagagatatgcgcaaaatcaataatgattttgatgttaaaactgttaaagagtggattaatagtGCggccgcatatactgatattgaaatgaatagattaggtaaataagaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacc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                                 "               %                                                                     &   K   +                                                                                                           
            	                           #            "                                 &                                    )                     +      F   !                                                                                                                &               #                     	                                                                      "                                             7ZD	: \[E_f; 8	dd nq

><5kxP \Ab/<  H/F`2f$pgs @ ]x0
b
D:>9B4	 zU Z p  9	l90GsvD  9 O    h@	}"Lc3  j).%	JSe!D 9d3'q		;
ey W or?z-6tlAQGf ,  _
	

K(~ ~ 'A+P/=N W s <JqT[r
TyA/ M 7]oB3?B j5ic0= 	 c  	B7s	+u0 I QGo2e% k    07Gf	%^	hpyt#  Mragk*U

	4GqLeP$TJ :3"T~(Yl*  I \	z

T &`c2AipF		;:Yw FOkQ
5?@H7CN_ A ^l	Hw;

1
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aV D	
Xk! n q  F:xI:2E 9	

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)#K Xd	,X_  /Y	/	

K"c|   WLk&	k			rf!*#   *`u6		,
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wTnE	i e I	P	'6} 4Z-fi=
z
 edz>  :f	bV	r		<
 Z  (MV% X  Xp	o	b{n.7Ev?8B K N3ux5
@2mH X I%\4	Ig  
s	
Y`vu[6Rs( - 0 F  `]c	h
 ;})aks   S;		5
]
 Mfp

Lnw=E"' I	T"   ,QJ>x!Xp% ]  'Pj (m(02	v		Od{0\   ABkl8DTdu8w !$),r[ wB% & 3  KS|		7q]6?HO0 t uS;U\
		s

gzz* =		AhU [m	=c4j ? w@a
C9a.T N 28g$		8ubC V ^IpZ'sSV@e H b  h*t1 />A   69j)eT
y

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j
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8	
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v{ 
 a,Y	= J  2 Hn	mu3 / E   L	:|`<r&  l7I&C/iv 2  RR	p\f> ZvfH?#		`
&8`Ri    !8Fig		x
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Vz<   1kK6_t
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@L:%K A%	MmU)V' v3#;U)			



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m  {;I}X


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#>	7
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a BRqVW( R G	T	b		;*x G'&9d3njiCZx kR


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+@AyGGO b*		BOiI` 
ICZu2
%IQM B mx$!
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{!s .X}


KRn  # M	F-Mp?m,2 p<H~>	Y


NYpSu)  ,z^


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  KEB|NP# UdT
 ?QV		u46 = `  
'VE<\R  dy.<^03		
>B, 	   		.Z ) 6 (u$,	f	8
b`a'Kh
 y  #l
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 QZn&
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!j{`{  >#~
[mN OV e		Bioz }Zmn  )b  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                 '                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                  '                                                                                                                                                                                                                              "                                       
                        	                  	                                    "                              _   L                     D                                                                                                                                                                            '                                                                                                                                                                                                                                                                                                                                                                   
                                                      '                                                                                    "             
        	      	            "          _ L       D   ca  d1  dM  dg  f  jk  o35u \[\1"
a1 $U!7OF	<		2	7Y%.n6?OmjItQ
7 I4X? vkvsT y0Z	Z  s^r	@.{3l r	u		l6$	|rV^ ruQAr  	9n}o0$w@n@x 	f	=|Uf $Q		7}dwDv"x	t63 .  	9{ ldn|k.(x	
,6X|	 g  f"\cK!, A$w Hln#lH l 	9F[.wc	+W^ixD #zDXG*{cp	V	- 0  >kxXp%<'q
Tx >~	l0v~pO8'- 0  k>xXp%LF^	;| -
[- 0  !p)r >kxX?Bp% >~XG*{cpLn='&c2iYw) 6 ($8
 W:9 M _1B+cM ' ,	f	`\a'B+cD	( 5 tg>P	5X
mXbv8	 :~%
+*OYh4 ;A
@? fCG%h:  k			rM4  7f	{>y	2)}V	 +%
	:~*!p)r ?B( 5 Dt	g RW(!bJ	Q	
V0U	MWWt

Xa 57 "L*s
WbRl4C Zn#X  Xp	o	b{n.7Ev?8BXd	,X_ l7I&C/iv 
s	
Y`vu[6Rs( $( #+y]zD0EoRs <JqT[r
TyA/  %?-x1gFgS	
w2 s ~   '<AJRlRq+PT/7I[	r
&p=N\Tf >CWy/iA/vQ gFj>aNU8gq=QS	a			[




*ARhBxFHw|YUdT
 Heo\b:	4
g

j!
6|4Yt
mT<		
E
!j{`{M 7]oB3?BX}*9yr'	
"+7%N   U28g ld,7IT$			
&08
C/iubCv5[mn
.>_n3  2&     y  <        <.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	U`%:eMe	q't-SЅ(zh)gneEVRQDa$Jb]yC)Df3UUJC6ghVFZmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          A  D  ;   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "\<?xml version="1.0"?><Features recycledIDs="27,28" nextValidID="29"><Feature recentID="2" name="carbamoyl phosphate synthase large" directionality="1" translationMW="11083.75" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="996" hitsStopCodon="1"><Segment range="4375-4674" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="carB" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-4674" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4674" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5136-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4900-4923" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4762-4781" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4740-4765" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4691-4720" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4688-4716" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4684-4719" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4677-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  z<?xml version="1.0"?><Primers recycledIDs="7,6,5,4,0" nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4711-4739" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4711-4739" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4711-4739" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4711-4739" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4761-4780" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4761-4780" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4761-4780" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4761-4780" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="22:11:16">2019.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -u     &          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	6ghVFZmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="0,1,2,27,28" nextValidID="29"><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4836-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4600-4623" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4462-4481" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4440-4465" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4391-4420" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4388-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4384-4419" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4377-4419" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  z<?xml version="1.0"?><Primers recycledIDs="7,6,5,4,0" nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4411-4439" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4411-4439" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4411-4439" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4411-4439" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4461-4480" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4461-4480" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4461-4480" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4461-4480" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="22:11:6">2019.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  3     y  9        9.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	%{i]fd'^K]yV	%Ք`U5G5humM	UfUmVu&`]A͙%e[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%          >  A  8   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="hypothetical protein" directionality="1" translationMW="11507.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="995" maxRunOn="4716" maxFusedRunOn="4716"><Segment range="4375-4671" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="FTL_0125" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-4671" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="hypothetical protein" directionality="1" translationMW="312.39" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4699" maxFusedRunOn="4699"><Segment range="4664-4671" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0124" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4664-4671" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4671" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5133-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4897-4920" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4759-4778" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4737-4762" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4688-4717" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4685-4713" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4681-4716" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4674-4716" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  z<?xml version="1.0"?><Primers recycledIDs="7,6,5,4,0" nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4708-4736" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4708-4736" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4708-4736" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4708-4736" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4758-4777" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4758-4777" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4758-4777" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4758-4777" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="22:10:59">2019.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  3{     y  <        <.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	%{i]fd'^K]yV	%Ք`U5G5humM	UfUmVu&`]AS6ghVFZmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          A  D  ;   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="hypothetical protein" directionality="1" translationMW="11507.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="995" hitsStopCodon="1"><Segment range="4375-4674" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="FTL_0125" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-4674" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="hypothetical protein" directionality="1" translationMW="426.49" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4702" maxFusedRunOn="4702"><Segment range="4664-4674" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0124" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4664-4674" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4674" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5136-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4900-4923" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4762-4781" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4740-4765" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4691-4720" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4688-4716" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4684-4719" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4677-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  z<?xml version="1.0"?><Primers recycledIDs="7,6,5,4,0" nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4711-4739" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4711-4739" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4711-4739" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4711-4739" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4761-4780" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4761-4780" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4761-4780" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4761-4780" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="22:8:23">2019.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -o     &          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	6ghVFZmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="0,1,2" nextValidID="27"><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4836-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4600-4623" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4462-4481" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4440-4465" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4391-4420" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4388-4416" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4384-4419" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4377-4419" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  z<?xml version="1.0"?><Primers recycledIDs="7,6,5,4,0" nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4411-4439" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4411-4439" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4411-4439" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4411-4439" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4461-4480" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4461-4480" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4461-4480" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4461-4480" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="22:8:15">2019.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  =Y     y  ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>          @  C  :   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5135-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4899-4922" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4761-4780" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4739-4764" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4690-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4687-4715" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4683-4718" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4676-4718" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="2" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4668-4673" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="4718" maxFusedRunOn="4718"><Segment range="4375-4673" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4673" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL213" sequence="ctagaggatccccgggtacCaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:11:36Z"><BindingSite location="4354-4377" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="4354-4377" bases="ctagaggatccccgggtacCaagg"/></BindingSite><BindingSite simplified="1" location="4354-4377" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="4354-4377" bases="ctagaggatccccgggtacCaagg"/></BindingSite></Primer><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4710-4738" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4710-4738" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4710-4738" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4710-4738" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  e<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="22:8:8">2019.7.1</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  +                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	ZaB%*-QI)%zUW%U&i`&멹-:Q#:WVZAQV+F}4'QTKNUUc!EfᩲQ[k[TQW;-j<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="27" name="/dnas_title=pKL02 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5635" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL02 Verified"/></Q></Feature><Feature recentID="26" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="25" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="24" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="23" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="note"><V text="P261 groESpCheck1"/></Q><Q name="label"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="22" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="note"><V text="P049 groES promoter F"/></Q><Q name="label"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="21" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="20" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q><Q name="label"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="note"><V text="P442 pEX_kanR_F"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="note"><V text="P443 pEX_kanR_R"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="17" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="16" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="note"><V text="P441 pEX_ckR"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="15" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q><Q name="label"><V text="sacB"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q><Q name="label"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="10" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q><Q name="label"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="9" name="FTL_1743 fragment" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4873" color="#993366" type="standard"/><Q name="note"><V text="FTL_1743 fragment"/></Q><Q name="label"><V text="FTL_1743 fragment"/></Q></Feature><Feature recentID="8" name="P002 FTL_1743fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4401" color="#a020f0" type="standard"/><Q name="note"><V text="P002 FTL_1743fragF"/></Q><Q name="label"><V text="P002 FTL_1743fragF"/></Q></Feature><Feature recentID="7" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4848-4918" color="#a020f0" type="standard"/><Q name="note"><V text="P001 FTL_1743fragR"/></Q><Q name="label"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="6" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4883-4918" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="5" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4887-4915" color="#a020f0" type="standard"/><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="4" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4890-4919" color="#a020f0" type="standard"/><Q name="note"><V text="P226 SP_pKL02_F"/></Q><Q name="label"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="3" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4939-4964" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q><Q name="label"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="2" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4961-4980" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q><Q name="label"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="1" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5099-5122" color="#a020f0" type="standard"/><Q name="note"><V text="P227 SP_pKL02_R"/></Q><Q name="label"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5335-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q><Q name="label"><V text="ColE origin"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  q<Notes>
<UUID>ab50ccb3-a294-49b1-80d4-d64540352dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="19:11:25">2019.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        {  A        Af	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<          
  4  =  @  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  ^<?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-309" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="321" maxFusedRunOn="321"><Segment range="11-309" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="304-309" color="#a6acb3" type="standard"/></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="1" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="2" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="3" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>395d1b84-d57b-4633-89b7-4c0e12448ebb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:10:51">2019.4.9</Created>
<LastModified UTC="19:12:24">2019.4.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <?xml version="1.0"?><HistoryTree><Node name="pKR41 pEX_FTL_0029-V.dna" type="DNA" seqLen="5433" ID="9" circular="1" operation="remove"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4671..5349" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4671..5349" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4671" val2="4673"/><Node name="pKR40 pEX_FTL_0029-V.dna" type="DNA" seqLen="5436" ID="8" circular="1" resurrectable="1" operation="insert"><Features><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4900-4923" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4740-4765" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4677-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="carB" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4375-4674" color="#00e600" type="standard"/></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5136-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4674..5352" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/><ColorRange range="4671..4673" colors="white"/></TopStrand><BottomStrand><ColorRange range="4674..5352" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/><ColorRange range="4671..4673" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4374..4673" colors="red"/></TopStrand><BottomStrand><ColorRange range="4374..4673" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4374"/><Node name="pKR40 pEX_FTL_0029-V.dna" type="DNA" seqLen="5136" ID="7" circular="1" resurrectable="1" operation="remove"><Features><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4600-4623" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4440-4465" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4377-4419" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4836-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4374..5015" colors="blue-black"/><ColorRange range="3732..4373" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4374..5015" colors="blue-black"/><ColorRange range="3732..4373" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4374" val2="4670"/><Node name="pKR40 pEX_FTL_0029-V.dna" type="DNA" seqLen="5433" ID="6" circular="1" resurrectable="1" operation="remove"><Features><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4897-4920" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4737-4762" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4674-4716" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4375-4671" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5133-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4671..5349" colors="blue-black"/><ColorRange range="3695..4373" colors="black-red"/><ColorRange range="4374..4670" colors="white"/></TopStrand><BottomStrand><ColorRange range="4671..5349" colors="blue-black"/><ColorRange range="3695..4373" colors="black-red"/><ColorRange range="4374..4670" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4671..5349" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4671..5349" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4671" val2="4673"/><Node name="pKR39 pEX_FTL_0125-V.dna" type="DNA" seqLen="5436" ID="5" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4900-4923" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4740-4765" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4677-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4375-4674" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5136-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4674..5352" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/><ColorRange range="4671..4673" colors="white"/></TopStrand><BottomStrand><ColorRange range="4674..5352" colors="blue-black"/><ColorRange range="3992..4670" colors="black-red"/><ColorRange range="4671..4673" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4374..4673" colors="red"/></TopStrand><BottomStrand><ColorRange range="4374..4673" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="4374"/><Node name="pKR39 pEX_FTL_0125-V.dna" type="DNA" seqLen="5136" ID="4" circular="1" resurrectable="1" operation="remove" hideHistory="1"><Features><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4600-4623" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4440-4465" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4377-4419" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4836-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="4374..5015" colors="blue-black"/><ColorRange range="3732..4373" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="4374..5015" colors="blue-black"/><ColorRange range="3732..4373" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="4374" val2="4672"/><Node name="pKR39 pEX_FTL_0125-V.dna" type="DNA" seqLen="5435" ID="3" circular="1" resurrectable="1" operation="insertFragment" hideHistory="1"><Features><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4899-4922" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4739-4764" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4676-4718" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="4375-4673" color="#00e600" type="standard"/></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5135-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature></Features><RegeneratedSite name="KpnI" pos="4373" siteCount="1"/><RegeneratedSite name="NotI" pos="4675" siteCount="1"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4673..5351" colors="blue-black"/><ColorRange range="3695..4373" colors="black-red"/><ColorRange range="4374..4672" colors="white"/></TopStrand><BottomStrand><ColorRange range="4673..5351" colors="blue-black"/><ColorRange range="3695..4373" colors="black-red"/><ColorRange range="4374..4672" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="4373..4674" colors="red"/></TopStrand><BottomStrand><ColorRange range="4369..4678" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" name1="NotI" name2="KpnI" val1="4875" val2="4373" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="KpnI" name2="NotI" val1="9" val2="311" siteCount1="1" siteCount2="1"/><Node name="pKL02 pEX_FTL1743-VSVG_DsacB.dna" type="DNA" seqLen="5635" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5335-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="FTL_1743 fragment" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="4375-4873" color="#993366" type="standard"/><Q name="label"><V text="FTL_1743 fragment"/></Q><Q name="note"><V text="FTL_1743 fragment"/></Q></Feature><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0"><Segment range="4883-4918" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4939-4964" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5099-5122" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="4373..4874" colors="white"/></TopStrand><BottomStrand><ColorRange range="4369..4878" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node><Node name="temp.dna" type="DNA" seqLen="321" ID="2" circular="0" upstreamModification="Unmodified" downstreamModification="Unmodified" resurrectable="1" operation="amplifyFragment" hideHistory="1"><Features><Feature name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0"><Segment range="11-309" color="#00e600" type="standard"/></Feature></Features><Oligo name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"/><Oligo name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"/><Parameter name="polymerase" val="polymerase that creates blunt ends"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="9..310" colors="red"/><ColorRange range="0..8" colors="white"/><ColorRange range="311..320" colors="white"/></TopStrand><BottomStrand><ColorRange range="5..314" colors="red"/><ColorRange range="0..4" colors="white"/><ColorRange range="315..320" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="0..9" colors="red"/><ColorRange range="309..320" colors="red"/></TopStrand><BottomStrand><ColorRange range="0..9" colors="red"/><ColorRange range="309..320" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="amplify" val1="1479491" val2="1479789"/><Node name="LVSgenomeWithPrimers.dna" type="DNA" seqLen="1895994" ID="1" circular="1" operation="invalid"><Primers><HybridizationParams minContinuousMatchLen="15" allowMismatch="0" minMeltingTemperature="40"/><Primer recentID="0" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1479491-1479517" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="1479491-1479517" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="1" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1479763-1479789" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagcg" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="1479763-1479789" bases="taaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer></Primers><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1479491..1479789" colors="black"/><ColorRange range="1479790..1479490" colors="white"/></TopStrand><BottomStrand><ColorRange range="1479491..1479789" colors="black"/><ColorRange range="1479790..1479490" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></Node></Node></Node></Node></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="0" name="carbamoyl phosphate synthase large" directionality="1" translationMW="11083.75" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="996" maxRunOn="4716" maxFusedRunOn="4716"><Segment range="4375-4671" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LKENGIECKLFDKASDNIGNDQVHSTVKAIEAGEVDLLVNTSIRQELKISLALRRAAIMSRVSYVTTIGAFEAFVIAVRDMRKINNDFDVKTVKEWINS"/></Q></Feature><Feature recentID="1" name="carB" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4375-4671" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4671" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="3" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5133-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="4" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4897-4920" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="5" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4759-4778" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="6" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4737-4762" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="7" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4688-4717" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="8" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4685-4713" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="9" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4681-4716" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="10" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4674-4716" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="11" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="12" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="13" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="14" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="15" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="16" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="17" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="20" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="21" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="23" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="24" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="25" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="26" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL240" sequence="gattacgaattcttatttacctaatctat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-08-06T12:50:08Z"><BindingSite location="4699-4727" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="4699-4727" bases="gattacgaattcttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="4699-4727" boundStrand="1" annealedBases="gattacgaattcttatttacctaatctat" meltingTemperature="52"><Component hybridizedRange="4699-4727" bases="gattacgaattcttatttacctaatctat"/></BindingSite></Primer><Primer recentID="1" name="KROL222 pKR41_construct_R" sequence="atatgcggccGCactattaatccactctttaacagttttaa" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-01T23:34:25Z"><BindingSite location="4642-4682" boundStrand="1" annealedBases="atatgcggccGCactattaatccactctttaacagttttaa" meltingTemperature="64"><Component hybridizedRange="4642-4682" bases="atatgcggccGCactattaatccactctttaacagttttaa"/></BindingSite><BindingSite simplified="1" location="4642-4682" boundStrand="1" annealedBases="atatgcggccGCactattaatccactctttaacagttttaa" meltingTemperature="64"><Component hybridizedRange="4642-4682" bases="atatgcggccGCactattaatccactctttaacagttttaa"/></BindingSite></Primer><Primer recentID="2" name="KROL221 pKR41_construct_F" sequence="atatggtacCttaaaagaaaatggtattgaatgtaagct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-01T23:32:35Z"><BindingSite location="4368-4402" boundStrand="0" annealedBases="ggtacCttaaaagaaaatggtattgaatgtaagct" meltingTemperature="59"><Component bases="atat"/><Component hybridizedRange="4368-4402" bases="ggtacCttaaaagaaaatggtattgaatgtaagct"/></BindingSite><BindingSite simplified="1" location="4368-4402" boundStrand="0" annealedBases="ggtacCttaaaagaaaatggtattgaatgtaagct" meltingTemperature="59"><Component bases="atat"/><Component hybridizedRange="4368-4402" bases="ggtacCttaaaagaaaatggtattgaatgtaagct"/></BindingSite></Primer><Primer recentID="3" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-25T23:58:07Z"><BindingSite location="4708-4736" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4708-4736" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4708-4736" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4708-4736" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="4" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4758-4777" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4758-4777" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4758-4777" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="55"><Component hybridizedRange="4758-4777" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="5" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="6" name="KROL276 VSVG seq  primer new" sequence="caattaatgtgagttagctcactcatt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-11-04T16:02:14Z"><BindingSite location="4822-4848" boundStrand="1" annealedBases="caattaatgtgagttagctcactcatt" meltingTemperature="55"><Component hybridizedRange="4822-4848" bases="caattaatgtgagttagctcactcatt"/></BindingSite><BindingSite simplified="1" location="4822-4848" boundStrand="1" annealedBases="caattaatgtgagttagctcactcatt" meltingTemperature="55"><Component hybridizedRange="4822-4848" bases="caattaatgtgagttagctcactcatt"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="16:3:46">2019.11.4</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                                     HO  d              E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
