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r{8e;6 m E
8o	n
ER1R3  CZKOKS* d   _tXdhvDhO~?c%a> 
 a'Db J  2 H+	
/
@=u?|<;C' / E   Lw	
^

J`p.n`F/:.  l7		
dRt[%#Igs7w)W 2  RRj
M>*NV4R~!u& ZvfH?
Sy..z 7dt|eM4?Q,    !8FiN	gq5[zFe?g05$  l `n	DNc}C(   1kK6i	


-BQm$}_"f V=  u>h5m	Rpy-EAdycf#^!e#3>Ph   Nstst#0189l				uvwx$,MNO@4YZcwxVWX5LMN )23MN2=NOC u$2{l:XH	P		"%Pu~5[Y 6O[Al4O&   Oy,b&t+L			
 m_q*Fzi" A%	Mm;	##vN3\q5/2 v3#;U	I	Q		r]6\ ZDPP( 6|4Y
_Bm>E/ {;I}x
9YiQM&Q7BY JU\c&,A
!r( <   -;]2M	
tn`	0yV{'' x Pz'u
#N

iRZI-,HqU+ZGx;   e			-#+jHi# BhN>D^
_4] hO
a BRq(			f$crRk0" R <	

$"0	Ok]:*- G'&9d3njo	
R

5k7w(F*nt6 kR		M t!Q_j'' ?w4$rGVcJ		v
	s*!]!Sj- V<PE	4	:`^G[aEQu#lU  b0	mz7BV0Z ! 
ICZ	 	c


(C gwNnF$ B mx$				Sz@	WnB}:  _75O<e	6	@	c	

ntU`<bi|2mISAl+? .X}6df 7#  # y
[]b[Nt>8G4 p<H~>			Y
 d:FZjR''>Nj%C  ,z^$&8RV<`9"  $|!NT{JGzj. Z | 5]0'-		=}  KEB	#J a	|f Udz	
-|"} ?QVtlCsV. = `  
'u	
>o$
D|&  dy.<^03N	

wpa1XvW 2	a			&Yr@(   	ulz|8  ) 6 (u$h

+j4h0.rVyT  y  #O
:Jn-(,4=[i2>H  {vI[/~:\ QZnSWI<  K ]o$sd9DI{  Er f	s
3q   ,u			.},dk  d'gj C	`3{%Z GeGZj" 8 o     ci-?&EA 5^." + r ;*OS	8
H
'_K^^  iPr)	j
8I3P<n 215-' j  q![S	9		g+YLd.9  *_b1:@ENh	

x^iDsTrPSl =.Cx				
1%2z G-T%;E6Xl+_a$ S =	
e
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*'/=s8UY|?S0; "	 
?



Ici#
&n "  w4S


-l|)(CHM$  Heo\	p
6X058xq7pzc& \  'jk
~lpCHPn8tw+you[-  (:{pq	

[5G	*Vs U lS		
`Ipk4G  C	2N!Vng" 0WguQJR` D   c;M (  YE	
M-|>d|B4B x+z%sWK		Q
!ot+DL^"KZ](@k1LdyM, @~5LH|w

XA"Cp=.D" ; w :%d	M

s+/T*xU:! Q N8=QnQ
C/6\b)mj ja	s `Rk/Y^)  WB	
		5	?		
;alHR@Rkv6?MV**   "  oSF			%U_MF$8 { 4,{k<B|U` _  x&/t1	=XqWC Ymg
*3V.1=[  c\nc8pC   b,7
@2-8  i  )p0?ZDg	8	


7w]XhmKC1O-  J!		d





)H:bDWk%.  iz[

}ZW/o~4~4xoGLZ C B	_1bH:fUo6 X}*9y	L


r
n@{*=BoAcC~% ! nM7R						
$.r5?(
X  l%W)jAT<_
BD    (ao+>yf7	




|v?Va.n}W3VJjB$,. 86Q	A	
q	a#Gm?-qT>An^	Ah     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=#m@cd3 04ax	d			

,1 BNe[`xF#+8e]e!  		2,9M@N/ }/h Y!5b	

!CO_fuaG$!8Jfr% f 1y

)7F\ey'v	"$ *~@Y	e	-IgKQ^f  	A
z
8u?dA 5[mnc\O-  	\^5:HpK! $ V	3KebwZ[N9O1 C0	#
-
Z

;P=UvH(?"=9<s* qi!ZI6?	
egOkzKD' f="c<&		"D[k`Sg (b%:%  S~p		!G&g>R,   ;c	


%AWhz= 2_z}	*SaK(w&  g  -{g>


'8DkG] )K(H 
:$		9;%fYF
#:  AUZ	/p  +D}O){k%J_	gS$ %\K
.'R	a[ "|Hf		hLR 6Y^
1
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>1nEe  .1-		

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wYGQv~ L 64n6F	q
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.bty +~q WJ	 .A+IiK- V	

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u&/xV RK^	
m[!;Dt\*%   @4f1EyO:OZ/P}$ % #t+wL(QcDuWr) > 1}	$

'Vw
LU^bx0pz@#D& G a a (
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
tbv
UE1<  z	8hq2X  T["1@
,pYw gFg'			!^9) >Uqb3	u
l)Fi&' -N~#d	b			~
'ms5ThcfA|  MG =yX
Z#E\aZ&-;=s  T	"-e `			vo#k{? ( 5  tHm'h}9S  Jq+t	r
r * 7  )Rd%G
iJ]z$]Y'  <-^Mw		i
O*01-qy' W


&,Tnhr5TXi{>R/:W%m1^$   v
kBGO'psvUy  
If  v ${~	CK.c{)\w3?Kx-9 `m	P
TQL5>j.)]i ^ z  3w.s+;bUo{-#  h  h	

69\ 30   |)K
)';0 '.nw1	


0J"El,)^*7]\ !  |)g? 
(^EHJLIu Ulm	vYZ$ BRo;%	f<T9y<=Q$+;   =9		:gXJJG C[	&QoR   %?-U^	
FP $Bi	p *e	Ex V 
y*jJo'  "L*sJ[	%P0t :YUq|#  P|} z&K@T]rTw%Y`u8C  !gTT ,E~LTF}	t
l4e,~rh ' 4  s*s	j
# ]VLv	N/SNI#lx u v-lr	~BT
b-e\,  &f	

/!]+(6 T<	P8XI  >#~ITh		
i)Xnw S\$i e	O7MSw }g	(htuvklm  .  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                            1                                                      
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      	   
                                                                                                                     	                                                                                                                                         +               	                                                                                                               	                                      
   	                  	                              *               	                                                	               
                                       '   '      .   !         #            t      $   (         6   l                                                                                                                                                                       1                          
                                                                                                                                                                                                                                                                          	 
                                             	                                                       +     	                                         	              
 	      	          *     	                	     
             ' '  . !   #    t  $ (   6 l   l  v      &    
<u I	*r)*8J"/1 #q
^5zOR4ccccoXvS 'OR[dS<;o
~h"NWB
 5CI&Zc eDkDbzN	 y	(y	( r(*	
wQ{3^ -ry	C(H|o
obo
blB}o
|bb	r.	y$^ sT]1ruCwArW	(Fm 6y<d,}|S\#$]EOX<y		o
o|bof&		(=JG4/ $QF
yKmqu2EOSDX\Wt7\
	8(,"xh6tS	mpD" -.  
IE kOsdn6
k$(o

Fb g  f"\cK&			!eUd A$EO .
<:Zv|lZv l6y	 [e
o1dixb6 #zi|&T]wI#C	#		$E	d- 0  	9F&ouGqx~<'R	?Zo"F_ >~T	
		:~p'_- 0  	9GqF&oux~LF,~r]	| -} x )- 0  !p)r	T
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cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7786-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7352-7356" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7318" maxFusedRunOn="7318"><Segment range="7352-7356" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8080" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers recycledIDs="30" nextValidID="32"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="29" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="28" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="27" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="26" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="25" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="24" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="23" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="22" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="21" name="KROL21 PriMnoC1FR2F" sequence="tgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:22">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ZZEfER)E9:%4Yʑ"ymE)]jj_vih;jhIVnV!mVўZVTdj۪tڑfenMgX%꭪j*[VUva^UjyU]eiIeYMZZQҡ4UZjelyYOWYY&mY*;R,IZCM|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;q<?xml version="1.0"?><Features nextValidID="39"><Feature recentID="38" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="37" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="36" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="35" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="33" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="32" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="31" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="30" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="29" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="28" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="27" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="26" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="25" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="22" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="6882" maxFusedRunOn="6882"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7916-7932" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8324-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7183-7458" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7159" maxFusedRunOn="7159"><Segment range="7183-7458" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6879-6887" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6879-6887" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7704-7894" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7673" maxFusedRunOn="7673"><Segment range="7704-7894" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6865-6869" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6865-6869" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8618" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  9$<?xml version="1.0"?><Primers nextValidID="21"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:17">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="4" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="3" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="product"><V text="beta-lactamase"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;Z9:[I^YJyӑYGVUSWyGzy[Whageiu7M9Սi]d+Ig)@z)fRI>9XVSRR+UcbZM9U]o)ShӮWFO^T-JlwʬQ$'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="33" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="32" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="31" name="P697 Ck_PriM_mpk1F" directionality="1" 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name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="27" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="26" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="25" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="22" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="6867" maxFusedRunOn="6867"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7378-7394" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7786-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7352-7356" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7318" maxFusedRunOn="7318"><Segment range="7352-7356" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8080" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers recycledIDs="30" nextValidID="32"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="29" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="28" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="27" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="26" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="25" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="24" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="23" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="22" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="21" name="KROL21 PriMnoC1FR2F" sequence="tgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:22">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ZZEfER)E9:%4Yʑ"ymE)]jj_vih;jhIVnV!mVўZVTdj۪tڑfenMgX%꭪j*[VUva^UjyU]eiIeYMZZQҡ4UZjelyYOWYY&mY*;R,IZCM|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8324-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7183-7458" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7159" maxFusedRunOn="7159"><Segment range="7183-7458" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6879-6887" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6879-6887" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7704-7894" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7673" maxFusedRunOn="7673"><Segment range="7704-7894" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6865-6869" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6865-6869" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8618" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  9$<?xml version="1.0"?><Primers nextValidID="21"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:17">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAbfV9jefId)jfbeIilߖV*_J^Yx4mM2JRfD}rM%MĩnjnQO6mZ7זUzwYHdfJH$c֪٨jQmhA4gXeJ%ͦ>NWWYIeRFjE6Jg^ڢ%qJaiNa^NvvV_c*Ine.ꑎej훦 &&Zh'j,QUdYa)]deX9>&-cV^UNf^֦[ZjٚDU)zSZOZ%+i6Yif&&j`&UimhjAh꛸*uEmuFIfMmZjVyTy)Y%"ZnڪjyUY%eWn6ꩽ%V:eXkEVTUjdեZje*ZcEZiIZOg]dyaՒaڕ"ě	+e=;Yt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="4" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="3" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="product"><V text="beta-lactamase"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  M               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAjckU6M8cRYMXUB^]`)F|^UF׎;PY8TF]UTQZC$yU&yVFoBlUS8Ql*YUYX/2jmڕ69^bGhf+#Q$X-f]TcxqyyyiK$uM*f8cwftd1I"UVN\;Z9:[I^YJyӑYGVUSWyGzy[Whageiu7M9Սi]d+Ig)@z)fRI>9XVSRR+UcbZM9U]o)ShӮWFO^T-JlwʬQ$'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  9<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7327-7351" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature><Feature recentID="41" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7025-7047" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="40" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6872-6898" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="39" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7287-7308" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="10" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7182-7204" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="9" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7035-7059" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="8" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7104-7132" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="7" name="hypothetical protein" directionality="1" translationMW="17717.02" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="304" maxRunOn="7384" maxFusedRunOn="7384"><Segment range="6865-7351" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6865-7351" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6865-7351" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="38" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="37" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="36" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="35" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="33" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="32" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="31" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="30" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="29" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="28" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="27" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="26" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="25" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="22" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="6867" maxFusedRunOn="6867"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7378-7394" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7786-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7352-7356" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7318" maxFusedRunOn="7318"><Segment range="7352-7356" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8080" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  P<?xml version="1.0"?><Primers recycledIDs="30" nextValidID="32"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="31" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6879-6905" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6879-6905" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="29" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6871-6897" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6871-6897" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="28" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7326-7356" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7326-7356" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="27" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7034-7058" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7058" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="26" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="25" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7103-7131" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7103-7131" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="24" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7181-7203" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7181-7203" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="23" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7024-7046" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7024-7046" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="22" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7286-7307" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7286-7307" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="21" name="KROL21 PriMnoC1FR2F" sequence="tgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6864-6897" boundStrand="0" annealedBases="tgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="59"><Component hybridizedRange="6864-6897" bases="tgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:22">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ZZEfER)E9:%4Yʑ"ymE)]jj_vih;jhIVnV!mVўZVTdj۪tڑfenMgX%꭪j*[VUva^UjyU]eiIeYMZZQҡ4UZjelyYOWYY&mY*;R,IZCM|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;q<?xml version="1.0"?><Features nextValidID="39"><Feature recentID="38" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="37" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="36" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="35" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="34" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="33" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="32" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="31" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="30" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="29" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="28" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="27" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="26" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="25" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="22" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="6882" maxFusedRunOn="6882"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7916-7932" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8324-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7183-7458" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7159" maxFusedRunOn="7159"><Segment range="7183-7458" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6879-6887" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6879-6887" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7704-7894" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7673" maxFusedRunOn="7673"><Segment range="7704-7894" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6865-6869" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6968" maxFusedRunOn="6968"><Segment range="6865-6869" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8618" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  9$<?xml version="1.0"?><Primers nextValidID="21"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="20" name="P707 PriM_unst1_R1" sequence="ctcttgcaccAGCgaacatGtcaatgttgcttctgtagtctacatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite><BindingSite simplified="1" location="6578-6624" boundStrand="1" annealedBases="ctcttgcaccgaacattcaatgttgcttctgtagtctacatag" meltingTemperature="58"><Component hybridizedRange="6615-6624" bases="ctcttgcacc"/><Component bases="AGC"/><Component hybridizedRange="6606-6611" bases="gaacat"/><Component bases="G"/><Component hybridizedRange="6578-6604" bases="tcaatgttgcttctgtagtctacatag"/></BindingSite></Primer><Primer recentID="19" name="P708 PriM_unst1_F1" sequence="gcaacattgaCatgttcGCTggtgcaagagtagttctttggaatg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite><BindingSite simplified="1" location="6595-6639" boundStrand="0" annealedBases="gcaacattgaatgttcggtgcaagagtagttctttggaatg" meltingTemperature="58"><Component hybridizedRange="6595-6604" bases="gcaacattga"/><Component bases="C"/><Component hybridizedRange="6606-6611" bases="atgttc"/><Component bases="GCT"/><Component hybridizedRange="6615-6639" bases="ggtgcaagagtagttctttggaatg"/></BindingSite></Primer><Primer recentID="18" name="P705 PriM_mtip2_R1" sequence="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6317" boundStrand="1" annealedBases="gatgttgttattaatctcatctctttca" meltingTemperature="53"><Component bases="ACCGCCACCTCCACCACCACCTCCACCACCTCCACCACC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="17" name="P706 PriM_mtip2_F2" sequence="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGGTaaagtagttgctgaacaagctaaacta" description="0"><BindingSite location="6345-6383" boundStrand="0" annealedBases="GGCGGTaaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGT"/><Component hybridizedRange="6345-6347" bases="GGC"/><Component hybridizedRange="6349-6350" bases="GG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite><BindingSite simplified="1" location="6356-6383" boundStrand="0" annealedBases="Taaagtagttgctgaacaagctaaacta" meltingTemperature="52"><Component bases="GGTGGTGGAGGTGGTGGAGGTGGTGGTGGAGGTGGCGG"/><Component hybridizedRange="6356-6383" bases="Taaagtagttgctgaacaagctaaacta"/></BindingSite></Primer><Primer recentID="16" name="P700 PriM_mtip1_R1" sequence="ctaaTTCTTCttgactttcaagCTCTTCgatgttgttattaatctcatctctttca" description="0"><BindingSite location="6290-6340" boundStrand="1" annealedBases="TTCTTttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaa"/><Component hybridizedRange="6336-6340" bases="TTCTT"/><Component bases="C"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite><BindingSite simplified="1" location="6290-6335" boundStrand="1" annealedBases="ttgactttcaaggatgttgttattaatctcatctctttca" meltingTemperature="51"><Component bases="ctaaTTCTTC"/><Component hybridizedRange="6324-6335" bases="ttgactttcaag"/><Component bases="CTCTTC"/><Component hybridizedRange="6290-6317" bases="gatgttgttattaatctcatctctttca"/></BindingSite></Primer><Primer recentID="15" name="P693 PriM_mpk_F2" sequence="GCAggtgcaGCAgtagttcttGCAaatggtcaatataacatcaatagcaaaga" description="0"><BindingSite location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite><BindingSite simplified="1" location="6615-6664" boundStrand="0" annealedBases="ggtgcaAgtagttcttaatggtcaatataacatcaatagcaaaga" meltingTemperature="54"><Component bases="GCA"/><Component hybridizedRange="6615-6620" bases="ggtgca"/><Component bases="GC"/><Component hybridizedRange="6623-6632" bases="Agtagttctt"/><Component bases="GCA"/><Component hybridizedRange="6636-6664" bases="aatggtcaatataacatcaatagcaaaga"/></BindingSite></Primer><Primer recentID="14" name="P701 PriM_mtip1_F2" sequence="atcGAAGAGcttgaaagtcaaGAAGAAttaggctggagaattaaagtagttgc" description="0"><BindingSite location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAGAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="G"/><Component hybridizedRange="6336-6339" bases="AAGA"/><Component hybridizedRange="6341-6367" bases="Attaggctggagaattaaagtagttgc"/></BindingSite><BindingSite simplified="1" location="6324-6367" boundStrand="0" annealedBases="cttgaaagtcaaAAttaggctggagaattaaagtagttgc" meltingTemperature="54"><Component bases="atcGAAGAG"/><Component hybridizedRange="6324-6335" bases="cttgaaagtcaa"/><Component bases="GAAG"/><Component hybridizedRange="6340-6367" bases="AAttaggctggagaattaaagtagttgc"/></BindingSite></Primer><Primer recentID="13" name="P692 PriM_mpk_R1" sequence="TGCaagaactacTGCtgcaccTGCgaacatatcaatgttgcttctgtagtc" description="0"><BindingSite location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite><BindingSite simplified="1" location="6585-6632" boundStrand="1" annealedBases="aagaactacTtgcaccgaacatatcaatgttgcttctgtagtc" meltingTemperature="55"><Component bases="TGC"/><Component hybridizedRange="6623-6632" bases="aagaactacT"/><Component bases="GC"/><Component hybridizedRange="6615-6620" bases="tgcacc"/><Component bases="TGC"/><Component hybridizedRange="6585-6611" bases="gaacatatcaatgttgcttctgtagtc"/></BindingSite></Primer><Primer recentID="12" name="P695 PriM_mpk_R3" sequence="catcagcTGCtagtgaaactgctttagttgtatcact" description="0"><BindingSite location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite><BindingSite simplified="1" location="6738-6774" boundStrand="1" annealedBases="catcagctagtgaaactgctttagttgtatcact" meltingTemperature="54"><Component hybridizedRange="6768-6774" bases="catcagc"/><Component bases="TGC"/><Component hybridizedRange="6738-6764" bases="tagtgaaactgctttagttgtatcact"/></BindingSite></Primer><Primer recentID="11" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="10" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="9" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="8" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="7" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="6" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="5" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="4" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="3" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="2" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="1" name="KROL19 PriMnoC1FR1F" sequence="aatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="aatttatgcattcaaaagcatagcagc" meltingTemperature="58"><Component hybridizedRange="6107-6133" bases="aatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="0" name="KROL20 PriMnoC1FR1R" sequence="aattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6863" boundStrand="1" annealedBases="aattgatagagccgttcttggcgaa" meltingTemperature="60"><Component hybridizedRange="6839-6863" bases="aattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:51:17">2018.11.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  6        "        ".	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAbfV9jefId)jfbeIilߖV*_J^Yx4mM2JRfD}rM%MĩnjnQO6mZ7זUzwYHdfJH$c֪٨jQmhA4gXeJ%ͦ>NWWYIeRFjE6Jg^ڢ%qJaiNa^NvvV_c*Ine.ꑎej훦 &&Zh'j,QUdYa)]deX9>&-cV^UNf^֦[ZjٚDU)zSZOZ%+i6Yif&&j`&UimhjAh꛸*uEmuFIfMmZjVyTy)Y%"ZnڪjyUY%eWn6ꩽ%V:eXkEVTUjdեZje*ZcEZiIZOg]dyaՒaڕ"ě	+e=;Yt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="15" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="11" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="10" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="9" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="8" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="7" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="6" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="5" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="4" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="3" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="product"><V text="beta-lactamase"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="2" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  co<?xml version="1.0"?><HistoryTree><Node name="pKR4 pEX_PriM_noC1.dna" type="DNA" seqLen="8082" ID="3" circular="1" operation="insert"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="6864..6865" colors="red"/></TopStrand><BottomStrand><ColorRange range="6864..6865" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="6864"/><Node name="pKR4 pEX_PriM_noC1.dna" type="DNA" seqLen="8080" ID="2" circular="1" resurrectable="1" operation="replace"><Features><Feature name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038"><Segment range="2704-4038" color="#993366" type="standard"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1919" color="#ccffcc" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7786-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6865-7351" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7378-7394" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7352-7356" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="V"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="6864..7350" colors="red"/></TopStrand><BottomStrand><ColorRange range="6864..7350" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="6864" val2="7888"/><Node name="pKR4 pEX_PriM_noC1.dna" type="DNA" seqLen="8618" ID="1" circular="1" resurrectable="1" operation="replace"><Features><Feature name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038"><Segment range="2704-4038" color="#993366" type="standard"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1919" color="#ccffcc" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="8324-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7183-7458" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KK*KR*LHYQHYY*YL*LVLLLVKI*IIILIF*ML*KMEKILLSLLISQIVNQR*KYLGNLVLNQ**FTMIL*FFQIPILLEIIHNTQMSQY"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7704-7894" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VPCCY*A*WRNY*RTT*GYSCDT*ANDIFK*LSYYLDEYWYVISLAIVDI*YSDWANY*REIL"/></Q></Feature><Feature name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7916-7932" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0"><Segment range="6879-6887" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LGM"/></Q></Feature><Feature name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0"><Segment range="6865-6869" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="G"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="6864..7888" colors="white"/></TopStrand><BottomStrand><ColorRange range="6864..7888" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="6107..6863" colors="red"/></TopStrand><BottomStrand><ColorRange range="6107..6863" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="6107" val2="7133"/><Node name="pKR4 pEX_PriM_noC1.dna" type="DNA" seqLen="8888" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0"><Segment range="7149-7157" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LGM"/></Q></Feature><Feature name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0"><Segment range="7131-7139" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YCG"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7974-8164" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VPCCY*A*WRNY*RTT*GYSCDT*ANDIFK*LSYYLDEYWYVISLAIVDI*YSDWANY*REIL"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="7453-7728" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KK*KR*LHYQHYY*YL*LVLLLVKI*IIILIF*ML*KMEKILLSLLISQIVNQR*KYLGNLVLNQ**FTMIL*FFQIPILLEIIHNTQMSQY"/></Q></Feature><Feature name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6113-6702" color="#993366" type="standard"/><Q name="EC_number"><V text="3.5.2.6"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="K*CVY*LQLYH*SRVSY*LLLN*MILLKIWKTNMMEKLASTLSTLMIKLTSNITKAITSLFVVFSSFY*LVQF*IMICIIKAS*IKKFQ*IKMILVNSDMLLLLLKTLVKL*QYHNLTTLLS*VIVLPVTY*LENLEVYKT*INL*KN*VITIL*LLLMNQKLIILSHIVI*IK*LQRLLLKIFIN*HLAIF*IKN"/></Q></Feature><Feature name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1919" color="#ccffcc" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038"><Segment range="2704-4038" color="#993366" type="standard"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="6107..7133" colors="white"/></TopStrand><BottomStrand><ColorRange range="6107..7133" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8082" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="41" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7320" maxFusedRunOn="7320"><Segment range="7354-7358" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="V"/></Q></Feature><Feature recentID="40" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7354-7358" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="39" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7788-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="38" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="37" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="36" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="35" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="33" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7380-7396" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="27" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6864" color="#00e600" type="standard"/></Feature><Feature recentID="26" name="hypothetical protein" directionality="1" translationMW="27863.55" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="51" maxRunOn="7386" maxFusedRunOn="7386"><Segment range="6108-6864" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IYAFKSIAADASNIQANGEKLARGFVLTNNGAVKIPNSIKPKNMYMKDQATAAQGLEKYRAERDEINNNIKKLESQKKLGWRIKVVAEQAKLKSINTKIDILKGIENGDKAYAEEKIAQFNIVKNITVNGPKAYFNDIAKPVLSHLNAAWDSATSLNYVDYRSNIDMFWGARVVLWNGQYNINSKDAATAIEFNDITNYLTIQTIKSLQGSDTTKAVSLYADANTLTYTVTTIGDLSSVQKKIASPRTALSI"/></Q></Feature><Feature recentID="25" name="P367 FTL0702seqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6266-6290" color="#8080ff" type="standard"/><Q name="note"><V text="P367 FTL0702seqF1"/></Q></Feature><Feature recentID="24" name="P429 FTL0702_VSVG_2R" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6363-6389" color="#50ff50" type="standard"/><Q name="note"><V text="P429 FTL0702_VSVG_2R"/></Q></Feature><Feature recentID="23" name="P435 RT_FTL0702-1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6570" color="#50ff50" type="standard"/><Q name="note"><V text="P435 RT_FTL0702-1F"/></Q></Feature><Feature recentID="22" name="P436 RT_FTL0702-1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6649-6670" color="#50ff50" type="standard"/><Q name="note"><V text="P436 RT_FTL0702-1R"/></Q></Feature><Feature recentID="21" name="P439 RT_FTL0702-3F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6768-6789" color="#50ff50" type="standard"/><Q name="note"><V text="P439 RT_FTL0702-3F"/></Q></Feature><Feature recentID="20" name="P440 RT_FTL0702-3R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6834-6853" color="#50ff50" type="standard"/><Q name="note"><V text="P440 RT_FTL0702-3R"/></Q></Feature><Feature recentID="19" name="P692 PriM_mpk_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6612" color="#50ff50" type="standard"/><Q name="note"><V text="P692 PriM_mpk_R1"/></Q></Feature><Feature recentID="18" name="P693 PriM_mpk_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6637-6665" color="#50ff50" type="standard"/><Q name="note"><V text="P693 PriM_mpk_F2"/></Q></Feature><Feature recentID="17" name="P697 Ck_PriM_mpk1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6551-6574" color="#50ff50" type="standard"/><Q name="note"><V text="P697 Ck_PriM_mpk1F"/></Q></Feature><Feature recentID="16" name="P700 PriM_mtip1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P700 PriM_mtip1_R1"/></Q></Feature><Feature recentID="15" name="P701 PriM_mtip1_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6343-6368" color="#50ff50" type="standard"/><Q name="note"><V text="P701 PriM_mtip1_F2"/></Q></Feature><Feature recentID="14" name="P704 CkPriM_mtip1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6496-6522" color="#50ff50" type="standard"/><Q name="note"><V text="P704 CkPriM_mtip1R"/></Q></Feature><Feature recentID="13" name="P706 PriM_mtip2_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6291-6318" color="#50ff50" type="standard"/><Q name="note"><V text="P706 PriM_mtip2_R1"/></Q></Feature><Feature recentID="12" name="P705 PriM_mtip2_F2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6358-6384" color="#50ff50" type="standard"/><Q name="note"><V text="P705 PriM_mtip2_F2"/></Q></Feature><Feature recentID="11" name="P707 PriM_unst1_R1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6579-6605" color="#50ff50" type="standard"/><Q name="note"><V text="P707 PriM_unst1_R1"/></Q></Feature><Feature recentID="10" name="P708 PriM_unst1_F1" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6616-6640" color="#50ff50" type="standard"/><Q name="note"><V text="P708 PriM_unst1_F1"/></Q></Feature><Feature recentID="9" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6867-7353" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6867-7353" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="hypothetical protein" directionality="1" translationMW="17717.02" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="304" maxRunOn="7386" maxFusedRunOn="7386"><Segment range="6867-7353" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EASLISIRTNTKVTTARNIINRLSNKKAVKPILRHLLNQTSIDDIILYDLDKLGDNWALKAATNAIRSTIGSDSILYVKGHTLMLISTSSMVDAFIKAIAENEIYKQVSDADRVLFGKNACNFTAAKNSNNPIVKAMIAASKQIAGQLPKGQVIDTVFEEKV"/></Q></Feature><Feature recentID="6" name="P368 FTL0702seqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7106-7134" color="#8080ff" type="standard"/><Q name="note"><V text="P368 FTL0702seqR1"/></Q></Feature><Feature recentID="5" name="P411 FTL0702sp_2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7037-7061" color="#50ff50" type="standard"/><Q name="note"><V text="P411 FTL0702sp_2R"/></Q></Feature><Feature recentID="4" name="P437 RT_FTL0702-2F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7184-7206" color="#50ff50" type="standard"/><Q name="note"><V text="P437 RT_FTL0702-2F"/></Q></Feature><Feature recentID="3" name="P438 RT_FTL0702-2R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7289-7310" color="#50ff50" type="standard"/><Q name="note"><V text="P438 RT_FTL0702-2R"/></Q></Feature><Feature recentID="2" name="P444 pKL75_F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6874-6900" color="#50ff50" type="standard"/><Q name="note"><V text="P444 pKL75_F"/></Q></Feature><Feature recentID="1" name="P698 Ck_PriM_mpk1R" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7027-7049" color="#50ff50" type="standard"/><Q name="note"><V text="P698 Ck_PriM_mpk1R"/></Q></Feature><Feature recentID="0" name="P702 PriM_mtip1_R2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7329-7353" color="#50ff50" type="standard"/><Q name="note"><V text="P702 PriM_mtip1_R2"/></Q></Feature></Features>
  =a<?xml version="1.0"?><Primers nextValidID="27"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="26" name="KROL150" sequence="gatatcctaaaaggtatcgaaaatggt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-14T14:07:35Z"><BindingSite location="6405-6431" boundStrand="0" annealedBases="gatatcctaaaaggtatcgaaaatggt" meltingTemperature="55"><Component hybridizedRange="6405-6431" bases="gatatcctaaaaggtatcgaaaatggt"/></BindingSite><BindingSite simplified="1" location="6405-6431" boundStrand="0" annealedBases="gatatcctaaaaggtatcgaaaatggt" meltingTemperature="55"><Component hybridizedRange="6405-6431" bases="gatatcctaaaaggtatcgaaaatggt"/></BindingSite></Primer><Primer recentID="25" name="KROL149" sequence="acggtgtcgataacttgacctttag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-14T14:06:46Z"><BindingSite location="7312-7336" boundStrand="1" annealedBases="acggtgtcgataacttgacctttag" meltingTemperature="59"><Component hybridizedRange="7312-7336" bases="acggtgtcgataacttgacctttag"/></BindingSite><BindingSite simplified="1" location="7312-7336" boundStrand="1" annealedBases="acggtgtcgataacttgacctttag" meltingTemperature="59"><Component hybridizedRange="7312-7336" bases="acggtgtcgataacttgacctttag"/></BindingSite></Primer><Primer recentID="0" name="KROL78" sequence="gcaattagatcaacaataggttctgat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7059-7085" boundStrand="0" annealedBases="gcaattagatcaacaataggttctgat" meltingTemperature="56"><Component hybridizedRange="7059-7085" bases="gcaattagatcaacaataggttctgat"/></BindingSite><BindingSite simplified="1" location="7059-7085" boundStrand="0" annealedBases="gcaattagatcaacaataggttctgat" meltingTemperature="56"><Component hybridizedRange="7059-7085" bases="gcaattagatcaacaataggttctgat"/></BindingSite></Primer><Primer recentID="1" name="KROL7" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7407-7426" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7407-7426" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7407-7426" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7407-7426" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="3" name="P821 PriM_mtip1_R3" sequence="atatGGTACCgtaactttggtattagttctgatactg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite><BindingSite simplified="1" location="6881-6907" boundStrand="1" annealedBases="gtaactttggtattagttctgatactg" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="6881-6907" bases="gtaactttggtattagttctgatactg"/></BindingSite></Primer><Primer recentID="4" name="P444 pKL75_F" sequence="atatGGTACCagcttaatcagtatcagaactaatacc" description="Contains KpnI site"><BindingSite location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6873-6899" boundStrand="0" annealedBases="agcttaatcagtatcagaactaatacc" meltingTemperature="55"><Component bases="atatGGTACC"/><Component hybridizedRange="6873-6899" bases="agcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="5" name="P702 PriM_mtip1_R2" sequence="atatGGTACCtactttctcttcgaaaacggtgtcg" description="Contains KpnI site"><BindingSite location="7328-7358" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7328-7358" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite><BindingSite simplified="1" location="7328-7358" boundStrand="1" annealedBases="GGTACCtactttctcttcgaaaacggtgtcg" meltingTemperature="63"><Component bases="atat"/><Component hybridizedRange="7328-7358" bases="GGTACCtactttctcttcgaaaacggtgtcg"/></BindingSite></Primer><Primer recentID="6" name="P411 FTL0702sp_2R" sequence="atatGGTACCgcatttgtagctgctttaagtgccc" description="Contains KpnI site"><BindingSite location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite><BindingSite simplified="1" location="7036-7060" boundStrand="1" annealedBases="gcatttgtagctgctttaagtgccc" meltingTemperature="61"><Component bases="atatGGTACC"/><Component hybridizedRange="7036-7060" bases="gcatttgtagctgctttaagtgccc"/></BindingSite></Primer><Primer recentID="7" name="P368 FTL0702seqR1" sequence="acttgtagatattaacattaaagtatgac" description="0"><BindingSite location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="1" annealedBases="acttgtagatattaacattaaagtatgac" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="acttgtagatattaacattaaagtatgac"/></BindingSite></Primer><Primer recentID="8" name="P430 CkFTL0702V_R" sequence="gtcatactttaatgttaatatctacaagt" description="0"><BindingSite location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite><BindingSite simplified="1" location="7105-7133" boundStrand="0" annealedBases="gtcatactttaatgttaatatctacaagt" meltingTemperature="52"><Component hybridizedRange="7105-7133" bases="gtcatactttaatgttaatatctacaagt"/></BindingSite></Primer><Primer recentID="9" name="P437 RT_FTL0702-2F" sequence="agcaagtaagtgatgctgataga" description="0"><BindingSite location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite><BindingSite simplified="1" location="7183-7205" boundStrand="0" annealedBases="agcaagtaagtgatgctgataga" meltingTemperature="55"><Component hybridizedRange="7183-7205" bases="agcaagtaagtgatgctgataga"/></BindingSite></Primer><Primer recentID="10" name="P698 Ck_PriM_mpk1R" sequence="gctttaagtgcccagttgtcgcc" description="0"><BindingSite location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite><BindingSite simplified="1" location="7026-7048" boundStrand="1" annealedBases="gctttaagtgcccagttgtcgcc" meltingTemperature="63"><Component hybridizedRange="7026-7048" bases="gctttaagtgcccagttgtcgcc"/></BindingSite></Primer><Primer recentID="11" name="P438 RT_FTL0702-2R" sequence="agttgtccggctatctgtttag" description="0"><BindingSite location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite><BindingSite simplified="1" location="7288-7309" boundStrand="1" annealedBases="agttgtccggctatctgtttag" meltingTemperature="56"><Component hybridizedRange="7288-7309" bases="agttgtccggctatctgtttag"/></BindingSite></Primer><Primer recentID="12" name="KROL21 PriMnoC1FR2F" sequence="atatCAATTGCtgaagctagcttaatcagtatcagaactaatacc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6856-6899" boundStrand="0" annealedBases="tatCAATTGCtgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="62"><Component bases="a"/><Component hybridizedRange="6856-6899" bases="tatCAATTGCtgaagctagcttaatcagtatcagaactaatacc"/></BindingSite><BindingSite simplified="1" location="6856-6899" boundStrand="0" annealedBases="tatCAATTGCtgaagctagcttaatcagtatcagaactaatacc" meltingTemperature="62"><Component bases="a"/><Component hybridizedRange="6856-6899" bases="tatCAATTGCtgaagctagcttaatcagtatcagaactaatacc"/></BindingSite></Primer><Primer recentID="13" name="P429 FTL0702_VSVG_2R" sequence="atatGAATTCagttgctgaacaagctaaactaaaaag" description="0"><BindingSite location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite><BindingSite simplified="1" location="6362-6388" boundStrand="0" annealedBases="agttgctgaacaagctaaactaaaaag" meltingTemperature="58"><Component bases="atatGAATTC"/><Component hybridizedRange="6362-6388" bases="agttgctgaacaagctaaactaaaaag"/></BindingSite></Primer><Primer recentID="14" name="P696 PriM_mpk_F4" sequence="ttcactaGCAgctgatgctaacacacttacatatac" description="0"><BindingSite location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite><BindingSite simplified="1" location="6768-6793" boundStrand="0" annealedBases="gctgatgctaacacacttacatatac" meltingTemperature="56"><Component bases="ttcactaGCA"/><Component hybridizedRange="6768-6793" bases="gctgatgctaacacacttacatatac"/></BindingSite></Primer><Primer recentID="15" name="P704 CkPriM_mtip1R" sequence="aatatcattaaaatacgccttaggacc" description="0"><BindingSite location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite><BindingSite simplified="1" location="6495-6521" boundStrand="1" annealedBases="aatatcattaaaatacgccttaggacc" meltingTemperature="55"><Component hybridizedRange="6495-6521" bases="aatatcattaaaatacgccttaggacc"/></BindingSite></Primer><Primer recentID="16" name="P367 FTL0702seqF1" sequence="ctcaaggtcttgagaaatacagagc" description="0"><BindingSite location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="0" annealedBases="ctcaaggtcttgagaaatacagagc" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="ctcaaggtcttgagaaatacagagc"/></BindingSite></Primer><Primer recentID="17" name="P413 CkFTL0702stp30R" sequence="gctctgtatttctcaagaccttgag" description="0"><BindingSite location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite><BindingSite simplified="1" location="6265-6289" boundStrand="1" annealedBases="gctctgtatttctcaagaccttgag" meltingTemperature="57"><Component hybridizedRange="6265-6289" bases="gctctgtatttctcaagaccttgag"/></BindingSite></Primer><Primer recentID="18" name="P697 Ck_PriM_mpk1F" sequence="cagcatgggattctgctactagtc" description="0"><BindingSite location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite><BindingSite simplified="1" location="6550-6573" boundStrand="0" annealedBases="cagcatgggattctgctactagtc" meltingTemperature="59"><Component hybridizedRange="6550-6573" bases="cagcatgggattctgctactagtc"/></BindingSite></Primer><Primer recentID="19" name="P439 RT_FTL0702-3F" sequence="tgctgatgctaacacacttaca" description="0"><BindingSite location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite><BindingSite simplified="1" location="6767-6788" boundStrand="0" annealedBases="tgctgatgctaacacacttaca" meltingTemperature="55"><Component hybridizedRange="6767-6788" bases="tgctgatgctaacacacttaca"/></BindingSite></Primer><Primer recentID="20" name="P436 RT_FTL0702-1R" sequence="cggcatctttgctattgatgtt" description="0"><BindingSite location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite><BindingSite simplified="1" location="6648-6669" boundStrand="1" annealedBases="cggcatctttgctattgatgtt" meltingTemperature="56"><Component hybridizedRange="6648-6669" bases="cggcatctttgctattgatgtt"/></BindingSite></Primer><Primer recentID="21" name="P440 RT_FTL0702-3R" sequence="ccgttcttggcgaagctatt" description="0"><BindingSite location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite><BindingSite simplified="1" location="6833-6852" boundStrand="1" annealedBases="ccgttcttggcgaagctatt" meltingTemperature="58"><Component hybridizedRange="6833-6852" bases="ccgttcttggcgaagctatt"/></BindingSite></Primer><Primer recentID="22" name="P435 RT_FTL0702-1F" sequence="cagcatgggattctgctact" description="0"><BindingSite location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite><BindingSite simplified="1" location="6550-6569" boundStrand="0" annealedBases="cagcatgggattctgctact" meltingTemperature="56"><Component hybridizedRange="6550-6569" bases="cagcatgggattctgctact"/></BindingSite></Primer><Primer recentID="23" name="KROL19 PriMnoC1FR1F" sequence="atatGGATCCaatttatgcattcaaaagcatagcagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6133" boundStrand="0" annealedBases="GGATCCaatttatgcattcaaaagcatagcagc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCaatttatgcattcaaaagcatagcagc"/></BindingSite><BindingSite simplified="1" location="6101-6133" boundStrand="0" annealedBases="GGATCCaatttatgcattcaaaagcatagcagc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCaatttatgcattcaaaagcatagcagc"/></BindingSite></Primer><Primer recentID="24" name="KROL20 PriMnoC1FR1R" sequence="atatGCaattgatagagccgttcttggcgaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6865" boundStrand="1" annealedBases="GCaattgatagagccgttcttggcgaa" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6839-6865" bases="GCaattgatagagccgttcttggcgaa"/></BindingSite><BindingSite simplified="1" location="6839-6865" boundStrand="1" annealedBases="GCaattgatagagccgttcttggcgaa" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6839-6865" bases="GCaattgatagagccgttcttggcgaa"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>1c592366-d9b8-45a5-988e-b0c99e1ab543</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:7:35">2019.5.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                            HO  d             E              Y?                                                                                                                                                                )<?xml version="1.0"?><CustomEnzymeSets/>
