	   SnapGene       `TATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAATCGATCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGATCTATACCCTTCAAGCTTTGAAAAATAAACTTAATTATTATATATGTTATTTAGCTAGTTTTTTTAATTAAAGTTAAAATCGAGAGCTTGTTTGACAAAAAAACAAAAAAATTTCTTGAAAATTTTTTTTTTGACTCAATATCTAGACTTGCAAGAGCTTGGAACTTTGAGATTGTTCTAAGATGCATACAAATTCAAAATGCTTAAACAAAAATAATTTAACAAAGGAGTAAGATTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTAATCGGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAGAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAATCGGCATTTTCTTTTGCGTTTTTATTTGTTAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTTTTCTTGCCTTTGATGTTCAGCAGGAAGCTAGGCGCAAACGTTGATTGTTTGTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGTTTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCGTTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTTGTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGTTAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAACAGATACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATCTGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGTAATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCCGTGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAATGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTTGGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGGCCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGAGCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTCCGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAAGAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTTGCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGTCAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGGATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGCGTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGTAAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACGATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGGCCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTGTGGACGAATCGAACTCAGGAACTTGATATTTTTCATTTTTTTGCTGTTCAGGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTCCTTATATGGCTTTTGGTTCGTTTCTTTCGCAAACGCTTGAGTTGCGCCTCCTGCCAGCAGTGCGGTAGTAAAGGTTAATACTGTTGCTTGTTTTGCAAACTTTTTGATGTTCATCGTTCATGTCTCCTTTTTTATGTACTGTGTTAGCGGTCTGCTTCTTCCAGCCCTCCTGTTTGAAGATGGCAAGTTAGTTACGCACAATAAAAAAAGACCTAAAATATGTAAGGGGTGACGCCAAAGTATACACTTTGCCCTTTACACATTTTAGGTCTTGCCTGCTTTATCAGTAACAAACCCGCGCGATTTACTTTTCGACCTCATTCTATTAGACTCTCGTTTGGATTGCAACTGGTCTATTTTCCTCTTTTGTTTGATAGAAAATCATAAAAGGATTTGCAGACTACGGGCCTAAAGAACTAAAAAATCTATCTGTTTCTTTTCATTCTCTGTATTTTTTATAGTTTCTGTTGCATGGGCATAAAGTTGCCTTTTTAATCACAATTCAGAAAATATCATAATATCTCATTTCACTAAATAATAGTGAACGGCAGGTATATGTGATGGGTTAAAAAGGATCGATCCTCTAGCTAGAGTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCTGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATGTCGATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCATATTTGAATGTATTTAGAAAAATAAACAAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGgatCcgaatacttgaacaaagtaaactatctcaaacacctgaagatagtttgaactgtcgccctgaagttaatccaaacaaagataactatgtgattggttatggcagccttatgaataaagattctagacagataacagttccaaatgcaacatatgccgcaccaattttagttagtagttttgaaaggttatgggcttcacgtggtgaaaaatctcgtgctacattcttacttgcagttccaaacaaaggttatgcaatgaatgcaatatattataaagccgatgctaaggatatctcagcaacagatctaagagaggcatcatattgtcgtgtaaaaataccgcgcaaagatattgtacctctaggtatcaagagtttaccaaaaggcgatttttggatgtatgttaaagattttaaagatgccgaatttccaaataaagactatcctatcttacaaacatatgctgatacttttatgacaggatgtctacaaactcaagccgaatttaacctaactgaatttggtaaactttgttttgatacaacctataactgggatctagcaaactggttatatgaccgttcaaaccctcgctatagcaggtattcacaagatactgagaaatatcgtccacaaattgataaaatcattcgccgcttaacttttgatgatgatcctttaGCggccgcatatactgatattgaaatgaatagattaggtaaataagaattCAATTGgtagcttttaaaatgaaaataacaactagaaaaatttgtatagcaccaatgcttgactggactgataggcactatcgttacatgatgcgtctaatcaccaggcatacaatgttatatactgaaatggttactttaaacgcaatcttacatggtaataaagaatttttgcttaaatataatcctcaagaaaatcctgtgacactacagttaggtggttgcgttccaaaagactttatcagttgtggcaagcttgccgaagaacttgactataatgaaattaacataaatgttggctgcccaagtgaaagagttaaaaaaggtaattttggcttatcattaatggcagagccagagcttgtagcagactgtgtcaaagctatgaaagataacctaaatatcccaatttcagttaaatgtcgtattggctatgatcataatgatagctatgaagaactatataattttgttgataagcaagttcaagcaggaatagattacctttgtattcatgcacgtaaaggttggcttagcggtctatcaccaaaagaaaatcgtaccatacctgagcttaaatatgatgttgtctacagtatcaaaaaagatttcccaaaccttgaactagggattaatggcggtattacaacaattcaagaaattcaacagcaccttaaatatgtggatagtgttatgataggtagagaagcatatcataaccGGTACCGAGCTCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACCCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAAC  0  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                    ,                                                            
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                                             	                                                            *   *   
   &   "         $                  "   d   c   -         2   n                                                                                                                                                                         ,                            
                                                                                                                                                                                                                                                                    
                                                                                                   ,                              "                          
 "      	  
        )  	  
  
               	                    * * 
 & "   $      " d c -   2 n   >O  ?  ?  ?  CY  H  Q;
[u I	*r)*W"/1 &#q
^GOR4ctcctcbjXvS 'OR[dS<;jo$B


 5I&Zc eDkDNz	 y	(y	( 0r*	
wQT{3^ZG ry	C|o
o/ o
/ l
+Jo
/ b	r.	g $^ sT+ruCwwArW	: 6p_<d,}|S\V $]EO% <F 		o
o/ of&		=J{4 $QF
yKmqu2EOSDX\$A)
	,"xh6t 	mu>=D .  
I kOdnn
k(o

FON/  g  f"\cK&			!eURIqB1 A$EO .
<:ZCI lZC l6p_	[e
o1di67aRxb6 #zizi hp YZ5		8	
XK AJ|&T]wI	#		$	d- 0  	9F&o>EK <'R	?Zo"F >~T	
		:~p'- 0  	9>F&oEK LF,~r]	| -} x )- 0  !p)r	T
vOX9F&o>sfE!K  >~T|	
&T]wIxW
Y<M&c2i

4j'E{) 6 ($h  } %-

id M y-B<T i
O1B ' 		
+4.^'B~	i
<OT1B DQ ( 5 t >Ps[X*
I%X0
:
0mD^	 :PLeOPOY6Z ;A	Z
e f]G6B	; 
:  k	V(i@9~Z   74Zz{>'G K|	 	~:PLeOP!p)rT
vOXsf!( 5 DtQ   RfF5xN!b!C_fa$0U	MWWt\			)
u&/x.[]# "L*sJ[	%P0t :t-g&">I X  Xp,		
0
:
T
>v=0XmyI<D2^hX[f
<p2< l7		
dRt[1tD$  
s]		(

v'.DCx)/\xN  
SB% #+y]zci7=	zhs <JqTR	{x)@NZpR|"GU   %?-U^	
FPW gFg'			!^N2 s ~   '<AJRlRq+PT/7Rj			
E
I

{dR(x)M@>NZpt*NRV|"4%G[@1btDU :$ ) B Q gFj>aNU8gq=Qbn'	3				Q
u
lC!/)s 6F^.:Enpi,!+-Ni4ekw[iz6Udz	
-|"} Heo\	p
6X058xqiG0    %?-P]VLU^|v		
} N/z&KS@T]rFPTwN%IY{-9BEW  * 7 >   %1?-)Rd%U^}		
$
G

'Vw
LU^FPbx0ipz@J#]1*y& W 6|4Y
_B{U[L?HT<	P8XI?&oM 7]oP	94ehX}*9y	L


r
n@{mvk?dT,VKN   U28g ld,7u	z					

:Td-R|."}_t}y[>D21?tSo8Di  $ 5[mnc\O-Y  K     J   _         _.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAY]RWwFJJ[yh)OTkїJc]}jJP!U#+
cXXfS9AFh%V|Q'BmDPƪ&)QTcūՕoWf8gabW<Zj	^g_xa4&QYVUΗa͙%eUYuUj&Mlam6ufxV
znYQjf[E[JB
2TzJ EyX!Q)UBZ鶖:$-STeYZbɠeNaEe(e5+SAdiP0E['Nb&Tj_y	]kQZMVbH	Mek%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>                   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #`<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="29" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="28" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="27" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7993-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="24" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="23" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="20" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="19" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="18" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7618-7637" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="17" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7596-7621" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="16" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="15" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="14" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="11" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6842-7438" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7662" maxFusedRunOn="7662"><Segment range="6856-7558" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-7558" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6842-7558" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6787" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="6" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6787" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="1" translationMW="26175.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="41" maxRunOn="6832" maxFusedRunOn="6832"><Segment range="6108-6787" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6782-6787" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6804-6833" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="2" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6801-6829" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="1" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6797-6832" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="0" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6790-6832" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature></Features>
  i<?xml version="1.0"?><Primers nextValidID="11"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL215" sequence="atatCAATTGgtagcttttaaaatgaaaataacaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-27T01:21:36Z"><BindingSite location="6836-6868" boundStrand="0" annealedBases="tCAATTgtagcttttaaaatgaaaataacaac" meltingTemperature="53"><Component bases="ata"/><Component hybridizedRange="6836-6841" bases="tCAATT"/><Component bases="G"/><Component hybridizedRange="6843-6868" bases="gtagcttttaaaatgaaaataacaac"/></BindingSite><BindingSite simplified="1" location="6836-6868" boundStrand="0" annealedBases="tCAATTgtagcttttaaaatgaaaataacaac" meltingTemperature="53"><Component bases="ata"/><Component hybridizedRange="6836-6841" bases="tCAATT"/><Component bases="G"/><Component hybridizedRange="6843-6868" bases="gtagcttttaaaatgaaaataacaac"/></BindingSite></Primer><Primer recentID="1" name="KROL214" sequence="atatCAATTGaattcttatttacctaatctattcatt" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T18:54:34Z"><BindingSite location="6810-6841" boundStrand="1" annealedBases="AATTGaattcttatttacctaatctattcatt" meltingTemperature="53"><Component bases="atatC"/><Component hybridizedRange="6810-6841" bases="AATTGaattcttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="6810-6841" boundStrand="1" annealedBases="AATTGaattcttatttacctaatctattcatt" meltingTemperature="53"><Component bases="atatC"/><Component hybridizedRange="6810-6841" bases="AATTGaattcttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="2" name="KROL213" sequence="atatGGATCCgaatacttgaacaaagtaaactatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T17:29:11Z"><BindingSite location="6101-6131" boundStrand="0" annealedBases="GGATCCgaatacttgaacaaagtaaactatc" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgaatacttgaacaaagtaaactatc"/></BindingSite><BindingSite simplified="1" location="6101-6131" boundStrand="0" annealedBases="GGATCCgaatacttgaacaaagtaaactatc" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgaatacttgaacaaagtaaactatc"/></BindingSite></Primer><Primer recentID="3" name="KROL216" sequence="atatGGTACCggttatgatatgcttctctacct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7535-7563" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacct" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7535-7563" bases="GGTACCggttatgatatgcttctctacct"/></BindingSite><BindingSite simplified="1" location="7535-7563" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacct" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7535-7563" bases="GGTACCggttatgatatgcttctctacct"/></BindingSite></Primer><Primer recentID="4" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="5" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7466-7492" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7466-7492" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7466-7492" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7466-7492" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="6" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="7" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6760-6798" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6760-6798" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6760-6798" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6760-6798" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="8" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6488-6514" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6488-6514" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6488-6514" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6488-6514" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="9" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="10" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="14:50:38">2019.7.3</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  K     J   _         _.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
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cXXfS9AFh%V|Q'BmDPƪ&)QTcūՕoWf8gabW<Zj	^g_xa4&QYVUΗa͙%eUYuUj&Mlam6ufxV
znYQjf[E[JB
2TzJ EyX!Q)UBZ鶖:$-STeYZbɠeNaEe(e5+SAdiP0E['Nb&Tj_y	]kQZMVbH	Mek%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>                   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #`<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="29" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="28" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="27" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7993-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="24" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="23" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="21" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="20" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="19" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="18" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7618-7637" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="17" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7596-7621" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="16" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="15" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="14" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="11" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6842-7438" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7662" maxFusedRunOn="7662"><Segment range="6856-7558" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-7558" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6842-7558" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6787" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="6" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6787" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="1" translationMW="26175.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="41" maxRunOn="6832" maxFusedRunOn="6832"><Segment range="6108-6787" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6782-6787" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6804-6833" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="2" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6801-6829" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="1" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6797-6832" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="0" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6790-6832" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature></Features>
  [<?xml version="1.0"?><Primers nextValidID="11"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL215" sequence="atatCAATTGgtagcttttaaaatgaaaataacaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-27T01:21:36Z"><BindingSite location="6838-6868" boundStrand="0" annealedBases="AATTgtagcttttaaaatgaaaataacaac" meltingTemperature="51"><Component bases="atatC"/><Component hybridizedRange="6838-6841" bases="AATT"/><Component bases="G"/><Component hybridizedRange="6843-6868" bases="gtagcttttaaaatgaaaataacaac"/></BindingSite><BindingSite simplified="1" location="6838-6868" boundStrand="0" annealedBases="AATTgtagcttttaaaatgaaaataacaac" meltingTemperature="51"><Component bases="atatC"/><Component hybridizedRange="6838-6841" bases="AATT"/><Component bases="G"/><Component hybridizedRange="6843-6868" bases="gtagcttttaaaatgaaaataacaac"/></BindingSite></Primer><Primer recentID="1" name="KROL214" sequence="atatCAATTGaattcttatttacctaatctattcatt" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T18:54:34Z"><BindingSite location="6810-6836" boundStrand="1" annealedBases="aattcttatttacctaatctattcatt" meltingTemperature="50"><Component bases="atatCAATTG"/><Component hybridizedRange="6810-6836" bases="aattcttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="6810-6836" boundStrand="1" annealedBases="aattcttatttacctaatctattcatt" meltingTemperature="50"><Component bases="atatCAATTG"/><Component hybridizedRange="6810-6836" bases="aattcttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="2" name="KROL213" sequence="atatGGATCCgaatacttgaacaaagtaaactatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T17:29:11Z"><BindingSite location="6101-6131" boundStrand="0" annealedBases="GGATCCgaatacttgaacaaagtaaactatc" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgaatacttgaacaaagtaaactatc"/></BindingSite><BindingSite simplified="1" location="6101-6131" boundStrand="0" annealedBases="GGATCCgaatacttgaacaaagtaaactatc" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgaatacttgaacaaagtaaactatc"/></BindingSite></Primer><Primer recentID="3" name="KROL216" sequence="atatGGTACCggttatgatatgcttctctacct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7535-7563" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacct" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7535-7563" bases="GGTACCggttatgatatgcttctctacct"/></BindingSite><BindingSite simplified="1" location="7535-7563" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacct" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7535-7563" bases="GGTACCggttatgatatgcttctctacct"/></BindingSite></Primer><Primer recentID="4" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="5" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7466-7492" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7466-7492" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7466-7492" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7466-7492" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="6" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="7" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6760-6798" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6760-6798" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6760-6798" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6760-6798" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="8" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6488-6514" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6488-6514" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6488-6514" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6488-6514" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="9" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="10" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="14:50:31">2019.7.3</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  N9     B   ^         ^.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAguI^n߅*?)oWTe!>YQGF]+ՍvcJI*B Vvz4WT)acYeOn]D6٢ȕY5Fh	CFQVTyn]ឩA^j_%z~eWdКFxEfZV:^a6ghVFZNVeבU7:Cy2)X)W5v	eEoow)(Qm(SXVebԅDU	jX4ODGNaQfjҕ&m9ԭM&7%BUlg:Va.tUQ~&uEk]7Y"d$E6m|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	               -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #`<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6789-6831" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="9" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6796-6831" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="8" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6800-6828" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="7" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6803-6832" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="6" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6781-6786" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="1" translationMW="26175.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="41" maxRunOn="6831" maxFusedRunOn="6831"><Segment range="6107-6786" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6786" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6786" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="30" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6841-7557" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6855-7557" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7661" maxFusedRunOn="7661"><Segment range="6855-7557" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6841-7437" color="#a6acb3" type="standard"/></Feature><Feature recentID="29" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="28" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="27" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="26" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="25" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="24" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7595-7620" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7617-7636" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="21" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="20" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="16" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="15" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7992-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="14" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="13" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="12,13" nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="KROL214" sequence="atatgaattcttatttacctaatctattcatttc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T18:54:34Z"><BindingSite location="6807-6835" boundStrand="1" annealedBases="aattcttatttacctaatctattcatttc" meltingTemperature="51"><Component bases="atatg"/><Component hybridizedRange="6807-6835" bases="aattcttatttacctaatctattcatttc"/></BindingSite><BindingSite simplified="1" location="6807-6835" boundStrand="1" annealedBases="aattcttatttacctaatctattcatttc" meltingTemperature="51"><Component bases="atatg"/><Component hybridizedRange="6807-6835" bases="aattcttatttacctaatctattcatttc"/></BindingSite></Primer><Primer recentID="3" name="KROL213a" sequence="atatGGATCCgaatacttgaacaaagtaaactatctca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T17:29:11Z"><BindingSite location="6105-6133" boundStrand="0" annealedBases="Cgaatacttgaacaaagtaaactatctca" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="6105-6133" bases="Cgaatacttgaacaaagtaaactatctca"/></BindingSite><BindingSite simplified="1" location="6105-6133" boundStrand="0" annealedBases="Cgaatacttgaacaaagtaaactatctca" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="6105-6133" bases="Cgaatacttgaacaaagtaaactatctca"/></BindingSite></Primer><Primer recentID="11" name="KROL216a" sequence="atatGGTACCggttatgatatgcttctctacctatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7531-7562" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7531-7562" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite><BindingSite simplified="1" location="7531-7562" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7531-7562" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite></Primer><Primer recentID="10" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7409-7436" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7409-7436" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7409-7436" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7409-7436" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="9" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7465-7491" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7465-7491" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7465-7491" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7465-7491" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="8" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7409-7436" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7409-7436" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7409-7436" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7409-7436" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="0" name="KROL216" sequence="atatGGTACCatgatcatagccaatacgacatttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:35Z"><BindingSite location="7250-7276" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7250-7276" bases="atgatcatagccaatacgacatttaac"/></BindingSite><BindingSite simplified="1" location="7250-7276" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7250-7276" bases="atgatcatagccaatacgacatttaac"/></BindingSite></Primer><Primer recentID="1" name="KROL215" sequence="atatGAATTCgtagcttttaaaatgaaaataacaactag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:34Z"><BindingSite location="6835-6870" boundStrand="0" annealedBases="tGAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="6835-6870" bases="tGAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite><BindingSite simplified="1" location="6835-6870" boundStrand="0" annealedBases="tGAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="6835-6870" bases="tGAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6759-6797" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6759-6797" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6759-6797" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6759-6797" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6487-6513" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6487-6513" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6487-6513" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6487-6513" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6760-6785" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6760-6785" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6760-6785" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6760-6785" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6760-6785" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6760-6785" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6760-6785" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6760-6785" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="20:5:5">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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}TU}Y(mUFX0uֵtߥf daٶ_	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%               -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #M<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6792-6834" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="9" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6799-6834" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="8" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6803-6831" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="7" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6806-6835" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="6" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6781-6789" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="1" translationMW="26175.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="41" hitsStopCodon="1"><Segment range="6107-6789" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6789" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6789" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="30" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6844-7560" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6858-7560" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7664" maxFusedRunOn="7664"><Segment range="6858-7560" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6844-7440" color="#a6acb3" type="standard"/></Feature><Feature recentID="29" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="28" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="27" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="26" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="25" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="24" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7598-7623" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7620-7639" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="21" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="20" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="16" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="15" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7995-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="14" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="13" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers recycledIDs="12,13" nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="KROL214" sequence="atatgaattcttatttacctaatctattcatttc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T18:54:34Z"><BindingSite location="6810-6838" boundStrand="1" annealedBases="aattcttatttacctaatctattcatttc" meltingTemperature="51"><Component bases="atatg"/><Component hybridizedRange="6810-6838" bases="aattcttatttacctaatctattcatttc"/></BindingSite><BindingSite simplified="1" location="6810-6838" boundStrand="1" annealedBases="aattcttatttacctaatctattcatttc" meltingTemperature="51"><Component bases="atatg"/><Component hybridizedRange="6810-6838" bases="aattcttatttacctaatctattcatttc"/></BindingSite></Primer><Primer recentID="3" name="KROL213a" sequence="atatGGATCCgaatacttgaacaaagtaaactatctca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T17:29:11Z"><BindingSite location="6105-6133" boundStrand="0" annealedBases="Cgaatacttgaacaaagtaaactatctca" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="6105-6133" bases="Cgaatacttgaacaaagtaaactatctca"/></BindingSite><BindingSite simplified="1" location="6105-6133" boundStrand="0" annealedBases="Cgaatacttgaacaaagtaaactatctca" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="6105-6133" bases="Cgaatacttgaacaaagtaaactatctca"/></BindingSite></Primer><Primer recentID="11" name="KROL216a" sequence="atatGGTACCggttatgatatgcttctctacctatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7534-7565" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7534-7565" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite><BindingSite simplified="1" location="7534-7565" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7534-7565" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite></Primer><Primer recentID="10" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7412-7439" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7412-7439" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7412-7439" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7412-7439" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="9" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7468-7494" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7468-7494" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7468-7494" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7468-7494" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="8" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7412-7439" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7412-7439" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7412-7439" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7412-7439" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="0" name="KROL216" sequence="atatGGTACCatgatcatagccaatacgacatttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:35Z"><BindingSite location="7253-7279" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7253-7279" bases="atgatcatagccaatacgacatttaac"/></BindingSite><BindingSite simplified="1" location="7253-7279" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7253-7279" bases="atgatcatagccaatacgacatttaac"/></BindingSite></Primer><Primer recentID="1" name="KROL215" sequence="atatGAATTCgtagcttttaaaatgaaaataacaactag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:34Z"><BindingSite location="6838-6873" boundStrand="0" annealedBases="tGAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="6838-6873" bases="tGAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite><BindingSite simplified="1" location="6838-6873" boundStrand="0" annealedBases="tGAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="6838-6873" bases="tGAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6759-6800" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="64"><Component hybridizedRange="6788-6800" bases="atatGCGGCCGCt"/><Component hybridizedRange="6759-6784" bases="aaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6759-6800" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="64"><Component hybridizedRange="6788-6800" bases="atatGCGGCCGCt"/><Component hybridizedRange="6759-6784" bases="aaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6487-6513" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6487-6513" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6487-6513" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6487-6513" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6760-6789" boundStrand="1" annealedBases="Cttataaaggatcatcatcaaaagttaagc" meltingTemperature="55"><Component bases="atatGGATC"/><Component hybridizedRange="6760-6789" bases="Cttataaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6760-6789" boundStrand="1" annealedBases="Cttataaaggatcatcatcaaaagttaagc" meltingTemperature="55"><Component bases="atatGGATC"/><Component hybridizedRange="6760-6789" bases="Cttataaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6760-6788" boundStrand="1" annealedBases="ttataaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component hybridizedRange="6760-6788" bases="ttataaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6760-6788" boundStrand="1" annealedBases="ttataaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component hybridizedRange="6760-6788" bases="ttataaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="19:59:51">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  F   
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAguI^n߅*?)oWTe!>YQGF]+ՍvcJI*B Vvz4WT)acYeOn]D6٢ȕY5Fh	CFQVTyn]ឩA^j_%z~eWdКFxEfZV:^aEUYuUj&Mlam6ufxV
znYQjf[E[JB
2TzJ EyX!Q)UBZ鶖:$-STeYZbɠeNaEe(e5+SAdiP0E['Nb&Tj_y	]kQZMVbH	Mek%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>        U   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="7,8,9" nextValidID="31"><Feature recentID="6" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6781-6789" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="1" translationMW="26175.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="41" hitsStopCodon="1"><Segment range="6107-6789" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6789" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6107-6789" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="30" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6794-7510" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6808-7510" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7614" maxFusedRunOn="7614"><Segment range="6808-7510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6794-7390" color="#a6acb3" type="standard"/></Feature><Feature recentID="29" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="28" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="27" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="26" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="25" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="24" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7548-7573" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7589" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="21" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="20" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="16" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="15" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7945-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="14" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="13" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,12,13" nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL213a" sequence="atatGGATCCgaatacttgaacaaagtaaactatctca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T17:29:11Z"><BindingSite location="6105-6133" boundStrand="0" annealedBases="Cgaatacttgaacaaagtaaactatctca" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="6105-6133" bases="Cgaatacttgaacaaagtaaactatctca"/></BindingSite><BindingSite simplified="1" location="6105-6133" boundStrand="0" annealedBases="Cgaatacttgaacaaagtaaactatctca" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="6105-6133" bases="Cgaatacttgaacaaagtaaactatctca"/></BindingSite></Primer><Primer recentID="11" name="KROL216a" sequence="atatGGTACCggttatgatatgcttctctacctatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7484-7515" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7484-7515" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite><BindingSite simplified="1" location="7484-7515" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7484-7515" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite></Primer><Primer recentID="10" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7362-7389" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7362-7389" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7362-7389" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7362-7389" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="9" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7418-7444" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7418-7444" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7418-7444" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7418-7444" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="8" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7362-7389" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7362-7389" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7362-7389" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7362-7389" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="0" name="KROL216" sequence="atatGGTACCatgatcatagccaatacgacatttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:35Z"><BindingSite location="7203-7229" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7203-7229" bases="atgatcatagccaatacgacatttaac"/></BindingSite><BindingSite simplified="1" location="7203-7229" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="7203-7229" bases="atgatcatagccaatacgacatttaac"/></BindingSite></Primer><Primer recentID="1" name="KROL215" sequence="atatGAATTCgtagcttttaaaatgaaaataacaactag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:34Z"><BindingSite location="6789-6823" boundStrand="0" annealedBases="GAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="6789-6823" bases="GAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite><BindingSite simplified="1" location="6789-6823" boundStrand="0" annealedBases="GAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="6789-6823" bases="GAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6759-6785" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagcg" meltingTemperature="55"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6759-6785" bases="taaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6759-6785" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagcg" meltingTemperature="55"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6759-6785" bases="taaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6487-6513" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6487-6513" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6487-6513" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6487-6513" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6760-6789" boundStrand="1" annealedBases="Cttataaaggatcatcatcaaaagttaagc" meltingTemperature="55"><Component bases="atatGGATC"/><Component hybridizedRange="6760-6789" bases="Cttataaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6760-6789" boundStrand="1" annealedBases="Cttataaaggatcatcatcaaaagttaagc" meltingTemperature="55"><Component bases="atatGGATC"/><Component hybridizedRange="6760-6789" bases="Cttataaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6760-6788" boundStrand="1" annealedBases="ttataaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component hybridizedRange="6760-6788" bases="ttataaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6760-6788" boundStrand="1" annealedBases="ttataaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component hybridizedRange="6760-6788" bases="ttataaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="19:55:38">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  I   	            .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAPƪ&)QTcūՕoWf8gabW<Zj	^g_xa4&QYVUΗa͙%eUYuUj&Mlam6ufxV
znYQjf[E[JB
2TzJ EyX!Q)UBZ鶖:$-STeYZbɠeNaEe(e5+SAdiP0E['Nb&Tj_y	]kQZMVbH	Mek%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>            <  C     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "O<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="30" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6466-7182" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6480-7182" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7286" maxFusedRunOn="7286"><Segment range="6480-7182" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6466-7062" color="#a6acb3" type="standard"/></Feature><Feature recentID="29" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="28" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="27" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="26" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="25" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="24" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7220-7245" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7242-7261" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="21" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="20" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="16" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="15" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7617-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="14" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="13" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="9" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6432-6461" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="8" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6429-6457" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="7" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6460" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="6" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6418-6460" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="5" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6410-6415" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="hypothetical protein" directionality="1" translationMW="12027.40" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="6460" maxFusedRunOn="6460"><Segment range="6117-6415" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6117-6415" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="3" nextValidID="12"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="11" name="KROL216a" sequence="atatGGTACCggttatgatatgcttctctacctatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7156-7187" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7156-7187" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite><BindingSite simplified="1" location="7156-7187" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacctatc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7156-7187" bases="GGTACCggttatgatatgcttctctacctatc"/></BindingSite></Primer><Primer recentID="10" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7034-7061" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7061" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7034-7061" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7034-7061" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="9" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7090-7116" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7090-7116" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7090-7116" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7090-7116" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="8" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7034-7061" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7034-7061" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7034-7061" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7034-7061" bases="tgatactgtagacaacatcatatttaag"/></BindingSite></Primer><Primer recentID="0" name="KROL216" sequence="atatGGTACCatgatcatagccaatacgacatttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:35Z"><BindingSite location="6875-6901" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="6875-6901" bases="atgatcatagccaatacgacatttaac"/></BindingSite><BindingSite simplified="1" location="6875-6901" boundStrand="1" annealedBases="atgatcatagccaatacgacatttaac" meltingTemperature="56"><Component bases="atatGGTACC"/><Component hybridizedRange="6875-6901" bases="atgatcatagccaatacgacatttaac"/></BindingSite></Primer><Primer recentID="1" name="KROL215" sequence="atatGAATTCgtagcttttaaaatgaaaataacaactag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:34Z"><BindingSite location="6461-6495" boundStrand="0" annealedBases="GAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="6461-6495" bases="GAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite><BindingSite simplified="1" location="6461-6495" boundStrand="0" annealedBases="GAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="6461-6495" bases="GAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite></Primer><Primer recentID="2" name="KROL213" sequence="ctagaggatccccgggtacCaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="6096-6119" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="6096-6119" bases="ctagaggatccccgggtacCaagg"/></BindingSite><BindingSite simplified="1" location="6096-6119" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="6096-6119" bases="ctagaggatccccgggtacCaagg"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6388-6426" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6388-6426" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6388-6426" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6388-6426" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6110-6142" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6110-6142" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6110-6142" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6110-6142" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="19:54:14">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  A               Λ.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAPƪ&)QTcūՕoWf8gabW<Zj	^g_xa4&QYVUΗa͙%eNVeבU7:Cy2)X)W5v	eEoow)(Qm(SXVebԅDU	jX4ODGNaQfjҕ&m|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	            <  B     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !=<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="28" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="27" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="26" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="25" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="24" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6939-6964" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="23" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6961-6980" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="22" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="21" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="20" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="17" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="16" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="15" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7336-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="14" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="13" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="9" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6432-6461" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="8" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6429-6457" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="7" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6425-6460" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="6" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6418-6460" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="5" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6410-6415" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="hypothetical protein" directionality="1" translationMW="12027.40" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="6460" maxFusedRunOn="6460"><Segment range="6117-6415" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6117-6415" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6479-6901" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="16165.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6934" maxFusedRunOn="6934"><Segment range="6479-6901" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6465-6901" color="#a6acb3" type="standard"/></Feature></Features>
  I<?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL216" sequence="atatGGTACCatgatcatagccaatacgacatttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:35Z"><BindingSite location="6874-6906" boundStrand="1" annealedBases="GGTACCatgatcatagccaatacgacatttaac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6874-6906" bases="GGTACCatgatcatagccaatacgacatttaac"/></BindingSite><BindingSite simplified="1" location="6874-6906" boundStrand="1" annealedBases="GGTACCatgatcatagccaatacgacatttaac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6874-6906" bases="GGTACCatgatcatagccaatacgacatttaac"/></BindingSite></Primer><Primer recentID="1" name="KROL215" sequence="atatGAATTCgtagcttttaaaatgaaaataacaactag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:34Z"><BindingSite location="6460-6494" boundStrand="0" annealedBases="GAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="6460-6494" bases="GAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite><BindingSite simplified="1" location="6460-6494" boundStrand="0" annealedBases="GAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="6460-6494" bases="GAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite></Primer><Primer recentID="2" name="KROL213" sequence="ctagaggatccccgggtacCaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="6096-6119" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="6096-6119" bases="ctagaggatccccgggtacCaagg"/></BindingSite><BindingSite simplified="1" location="6096-6119" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="6096-6119" bases="ctagaggatccccgggtacCaagg"/></BindingSite></Primer><Primer recentID="3" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="6452-6468" boundStrand="1" annealedBases="tacgaattcttatttac" meltingTemperature="40"><Component bases="ctatgacatgat"/><Component hybridizedRange="6452-6468" bases="tacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="6452-6468" boundStrand="1" annealedBases="tacgaattcttatttac" meltingTemperature="40"><Component bases="ctatgacatgat"/><Component hybridizedRange="6452-6468" bases="tacgaattcttatttac"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6388-6426" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6388-6426" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6388-6426" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6388-6426" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6110-6142" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6110-6142" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6110-6142" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6110-6142" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6389-6414" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6389-6414" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="19:50:36">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  !                 .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6839" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6545-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6170-6189" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6148-6173" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>afcff009-b1cb-4dd1-b062-af31a838e80c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="4:0:0">2018.7.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *                APƪ&)QTcūՕoWf8gabW<Zj	^g_xa4&QYVUΗa͙%e[b             >  A     -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="21-319" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="hypothetical protein" directionality="1" translationMW="12027.40" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="364" maxFusedRunOn="364"><Segment range="21-319" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-319" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="322-364" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="3" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="329-364" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="2" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="333-361" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="1" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="336-365" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="0" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="385-385" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature></Features>
  c<?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="293-318" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="293-318" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="293-318" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="293-318" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="1" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="293-318" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="293-318" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="293-318" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="293-318" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="2" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="14-46" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="14-46" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="14-46" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="14-46" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="3" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="292-330" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="292-330" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="292-330" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="292-330" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="4" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="356-384" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="356-384" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="356-384" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="356-384" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="5" name="KROL213" sequence="ctagaggatccccgggtacCaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="0-23" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="63"><Component hybridizedRange="0-23" bases="ctagaggatccccgggtacCaagg"/></BindingSite><BindingSite simplified="1" location="0-23" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="63"><Component hybridizedRange="0-23" bases="ctagaggatccccgggtacCaagg"/></BindingSite></Primer></Primers>
  f<Notes>
<UUID>f35569b8-ffcc-4c40-874f-6f8922a1d56a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:26:8">2019.6.26</Created>
<LastModified UTC="0:26:8">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  =[     y  ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>          @  C  :   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5135-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="ColE origin"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="25" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4899-4922" color="#a020f0" type="standard"/><Q name="label"><V text="P227 SP_pKL02_R"/></Q><Q name="note"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="24" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4761-4780" color="#a020f0" type="standard"/><Q name="label"><V text="P022 pEX18SeqR1"/></Q><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="23" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4739-4764" color="#a020f0" type="standard"/><Q name="label"><V text="P024 pEX18SeqR2"/></Q><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="22" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4690-4719" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="21" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4687-4715" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="20" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4683-4718" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="19" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4676-4718" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="18" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="label"><V text="P021 pEX18SeqF1"/></Q><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="label"><V text="P023 pEX18SeqF2"/></Q><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="label"><V text="sacB"/></Q><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="13" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P441 pEX_ckR"/></Q></Feature><Feature recentID="12" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P443 pEX_kanR_R"/></Q></Feature><Feature recentID="10" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type: PCR_primer"/></Q><Q name="note"><V text="P442 pEX_kanR_F"/></Q></Feature><Feature recentID="9" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="label"><V text="kanamycin resistance"/></Q><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="8" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="7" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="label"><V text="P049 groES promoter F"/></Q><Q name="note"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="6" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="label"><V text="P261 groESpCheck1"/></Q><Q name="note"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="5" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="4" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="3" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="2" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4668-4673" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="4718" maxFusedRunOn="4718"><Segment range="4375-4673" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4673" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL213" sequence="ctagaggatccccgggtacCaagg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="4354-4377" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="4354-4377" bases="ctagaggatccccgggtacCaagg"/></BindingSite><BindingSite simplified="1" location="4354-4377" boundStrand="0" annealedBases="ctagaggatccccgggtacCaagg" meltingTemperature="64"><Component hybridizedRange="4354-4377" bases="ctagaggatccccgggtacCaagg"/></BindingSite></Primer><Primer recentID="1" name="KROL214" sequence="ctatgacatgattacgaattcttatttac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:26:05Z"><BindingSite location="4710-4738" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4710-4738" bases="ctatgacatgattacgaattcttatttac"/></BindingSite><BindingSite simplified="1" location="4710-4738" boundStrand="1" annealedBases="ctatgacatgattacgaattcttatttac" meltingTemperature="53"><Component hybridizedRange="4710-4738" bases="ctatgacatgattacgaattcttatttac"/></BindingSite></Primer><Primer recentID="2" name="KROL7 Seq_pEX_2" sequence="tgtggaattgtgagcggata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite><BindingSite simplified="1" location="4760-4779" boundStrand="1" annealedBases="tgtggaattgtgagcggata" meltingTemperature="54"><Component hybridizedRange="4760-4779" bases="tgtggaattgtgagcggata"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="gttttcccagtcacgacgtt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite><BindingSite simplified="1" location="4289-4308" boundStrand="0" annealedBases="gttttcccagtcacgacgtt" meltingTemperature="57"><Component hybridizedRange="4289-4308" bases="gttttcccagtcacgacgtt"/></BindingSite></Primer><Primer recentID="4" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="4646-4684" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="4646-4684" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="4368-4400" boundStrand="0" annealedBases="GGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="4368-4400" bases="GGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="6" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="7" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="4647-4672" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="4647-4672" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>7852ecbe-8978-4cd2-93be-d12d97eb7626</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:12:30">2019.4.9</Created>
<LastModified UTC="0:11:37">2019.6.26</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  +               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;Ӌr>DfZ ql^^jM`Ȗ`&*f+*\J3M#	%Bxꡊ+b益'㮽?*`Ԥ7YUQVbPs&wv㩴W1kG*x.jꊆE[ePj4yQyUnn*궻J`6TꥷZVmnڷH\4&b
UAK163ODM?Cг<pА<+.'~B*Oo:?OODk=ŔHQAw?Ko#P`f|U[9MS8⨁fǁ]XVGD|q8<H
=E3!8@"7F2PEYm٨XUD\P`㥨cҦ?=<+V2W]WǄcҿy9*޸J`B=Z⩴|댮mnt;=8xl#sӃ 5iJ\
rW=#бD	ZaB%*-QI)%zUW%U&i`&멹-:Q#:WVZAQV+F}4'QTKNUUc!EfᩲQ[k[TQW;-j<ٞXYBVQk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="27" name="/dnas_title=pKL02 Verified" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-5635" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL02 Verified"/></Q></Feature><Feature recentID="26" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q><Q name="label"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="25" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q><Q name="label"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="24" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q><Q name="label"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="23" name="P261 groESpCheck1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="826-859" color="#a020f0" type="standard"/><Q name="note"><V text="P261 groESpCheck1"/></Q><Q name="label"><V text="P261 groESpCheck1"/></Q></Feature><Feature recentID="22" name="P049 groES promoter F" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="859-883" color="#a6acb3" type="standard"/><Q name="note"><V text="P049 groES promoter F"/></Q><Q name="label"><V text="P049 groES promoter F"/></Q></Feature><Feature recentID="21" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="20" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q><Q name="label"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="19" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1259-1283" color="#a6acb3" type="standard"/><Q name="note"><V text="P442 pEX_kanR_F"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1531-1553" color="#a6acb3" type="standard"/><Q name="note"><V text="P443 pEX_kanR_R"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="17" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q><Q name="label"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="16" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2007-2030" color="#a6acb3" type="standard"/><Q name="note"><V text="P441 pEX_ckR"/></Q><Q name="label"><V text="nonstandard type: PCR_primer"/></Q></Feature><Feature recentID="15" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2119" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q><Q name="label"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="14" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2120-2383" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q><Q name="label"><V text="sacB"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2648-2674" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q><Q name="label"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3367-3391" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q><Q name="label"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4266-4289" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q><Q name="label"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="10" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4290-4309" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q><Q name="label"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="9" name="FTL_1743 fragment" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4873" color="#993366" type="standard"/><Q name="note"><V text="FTL_1743 fragment"/></Q><Q name="label"><V text="FTL_1743 fragment"/></Q></Feature><Feature recentID="8" name="P002 FTL_1743fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4375-4401" color="#a020f0" type="standard"/><Q name="note"><V text="P002 FTL_1743fragF"/></Q><Q name="label"><V text="P002 FTL_1743fragF"/></Q></Feature><Feature recentID="7" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4848-4918" color="#a020f0" type="standard"/><Q name="note"><V text="P001 FTL_1743fragR"/></Q><Q name="label"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="6" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4883-4918" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="5" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4887-4915" color="#a020f0" type="standard"/><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="4" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4890-4919" color="#a020f0" type="standard"/><Q name="note"><V text="P226 SP_pKL02_F"/></Q><Q name="label"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="3" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4939-4964" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q><Q name="label"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="2" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4961-4980" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q><Q name="label"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="1" name="P227 SP_pKL02_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5099-5122" color="#a020f0" type="standard"/><Q name="note"><V text="P227 SP_pKL02_R"/></Q><Q name="label"><V text="P227 SP_pKL02_R"/></Q></Feature><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5335-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q><Q name="label"><V text="ColE origin"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  q<Notes>
<UUID>ab50ccb3-a294-49b1-80d4-d64540352dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>synthetic circular DNA</Description>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="19:11:25">2019.4.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        {  A        Af	jbbF<ZYQuvcz.u{Sũ~^*unM^
f+W9Bku/uhe[k<zL<          
  4  =  @  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  ^<?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-309" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="hypothetical protein" directionality="1" translationMW="12027.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="168" maxRunOn="321" maxFusedRunOn="321"><Segment range="11-309" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="304-309" color="#a6acb3" type="standard"/></Feature></Features>
  
<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="tta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="1" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="283-308" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="53"><Component bases="atatGGATCCtta"/><Component hybridizedRange="283-308" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="2" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="0-36" boundStrand="0" annealedBases="atatGGTACCaaggcgatttttggatgtatgttaaag" meltingTemperature="62"><Component hybridizedRange="0-36" bases="atatGGTACCaaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="3" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="282-320" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="282-320" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>395d1b84-d57b-4633-89b7-4c0e12448ebb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="19:10:51">2019.4.9</Created>
<LastModified UTC="19:12:24">2019.4.9</LastModified>
<CreatedBy>Kathryn Ramsey</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ^                fNVeבU7:Cy2)X)W5v	eEoow)(Qm(SXVebԅDU	jX4ODGNaQfjҕ&m}&          
         -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  =<?xml version="1.0"?><Features nextValidID="4"><Feature recentID="3" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-445" color="#a6acb3" type="standard"/></Feature><Feature recentID="2" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="16165.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="455" maxFusedRunOn="454"><Segment range="23-445" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-445" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-445" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL215" sequence="atatGAATTCgtagcttttaaaatgaaaataacaactag" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:34Z"><BindingSite location="0-38" boundStrand="0" annealedBases="atatGAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="57"><Component hybridizedRange="0-38" bases="atatGAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite><BindingSite simplified="1" location="0-38" boundStrand="0" annealedBases="atatGAATTCgtagcttttaaaatgaaaataacaactag" meltingTemperature="57"><Component hybridizedRange="0-38" bases="atatGAATTCgtagcttttaaaatgaaaataacaactag"/></BindingSite></Primer><Primer recentID="1" name="KROL216" sequence="atatGGTACCatgatcatagccaatacgacatttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T00:25:35Z"><BindingSite location="418-454" boundStrand="1" annealedBases="atatGGTACCatgatcatagccaatacgacatttaac" meltingTemperature="61"><Component hybridizedRange="418-454" bases="atatGGTACCatgatcatagccaatacgacatttaac"/></BindingSite><BindingSite simplified="1" location="418-454" boundStrand="1" annealedBases="atatGGTACCatgatcatagccaatacgacatttaac" meltingTemperature="61"><Component hybridizedRange="418-454" bases="atatGGTACCatgatcatagccaatacgacatttaac"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>9311e23b-c122-4502-84be-a44172822887</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:25:40">2019.6.26</Created>
<LastModified UTC="0:25:40">2019.6.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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3OO@l,MEIɛHh76(ޗ\!m㩩NKf\/Wd f%`oq'"&l  .IOU@GA1BC(?]~/!팄@q|Z]zߑB<wցT\ENgJ< HW`y]!$HdmܪuQ`iGO/EE@d Ai]zFqWzZc&*.8+|>ӊO$!Cb+]/TA{ .Po,hqZC.;^2G`iLIC~ifI>|n2}2ռKt*V)jIjrK5 ł8,-Gi4DޙUQ+7a}8⮠G|?_ֲpN9f{'zznLIYQ}[#lo*<B29os Oc'[WX=)Q[M		zg݋'ԀS(S[ [ &ߖ^gؿ3zJfڿ *HRM<sA3i\wY x<*vy"r[&!6z8d>N"oMcrlM#YEȺrj^]Tco=:3x/FpVB"*7r0|4lY.ZZ F<0;89ނ8		7
if)~7 fc= )sAd:/[-./VљVa1O~]Bg}mozˬQx^5۹Tm@
!|A!P+UB[:du"F_9܀atC!h2M=O3Lb p^AK    "\A  -g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %x<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="0" name="P001 FTL_1743fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6790-6832" color="#a020f0" type="standard"/><Q name="label"><V text="P001 FTL_1743fragR"/></Q><Q name="note"><V text="P001 FTL_1743fragR"/></Q></Feature><Feature recentID="1" name="signal peptide" type="sig_peptide" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6797-6832" color="#cc99b2" type="standard"/><Q name="note"><V text="VSVG"/></Q></Feature><Feature recentID="2" name="P035 pEX18integrationVSVG" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6801-6829" color="#a020f0" type="standard"/><Q name="label"><V text="P035 pEX18integrationVSVG"/></Q><Q name="note"><V text="P035 pEX18integrationVSVG"/></Q></Feature><Feature recentID="3" name="P226 SP_pKL02_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6804-6833" color="#a020f0" type="standard"/><Q name="label"><V text="P226 SP_pKL02_F"/></Q><Q name="note"><V text="P226 SP_pKL02_F"/></Q></Feature><Feature recentID="4" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6782-6787" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="hypothetical protein" directionality="1" translationMW="26175.67" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="41" maxRunOn="6832" maxFusedRunOn="6832"><Segment range="6108-6787" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="ILEQSKLSQTPEDSLNCRPEVNPNKDNYVIGYGSLMNKDSRQITVPNATYAAPILVSSFERLWASRGEKSRATFLLAVPNKGYAMNAIYYKADAKDISATDLREASYCRVKIPRKDIVPLGIKSLPKGDFWMYVKDFKDAEFPNKDYPILQTYADTFMTGCLQTQAEFNLTEFGKLCFDTTYNWDLANWLYDRSNPRYSRYSQDTEKYRPQIDKIIRRLTFDDDPL"/></Q></Feature><Feature recentID="6" name="FTL_1548" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6787" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6787" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6842-7558" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="9" name="FTL_1549" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6856-7558" color="#00e600" type="standard"/></Feature><Feature recentID="10" name="tRNA-dihydrouridine synthase A" directionality="1" translationMW="26880.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7662" maxFusedRunOn="7662"><Segment range="6856-7558" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKITTRKICIAPMLDWTDRHYRYMMRLITRHTMLYTEMVTLNAILHGNKEFLLKYNPQENPVTLQLGGCVPKDFISCGKLAEELDYNEININVGCPSERVKKGNFGLSLMAEPELVADCVKAMKDNLNIPISVKCRIGYDHNDSYEELYNFVDKQVQAGIDYLCIHARKGWLSGLSPKENRTIPELKYDVVYSIKKDFPNLELGINGGITTIQEIQQHLKYVDSVMIGREAYHN"/></Q></Feature><Feature recentID="11" name="DFTL_1548_FR2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6842-7438" color="#a6acb3" type="standard"/></Feature><Feature recentID="12" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="13" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="14" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="15" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="17" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7596-7621" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="18" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7618-7637" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="19" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="20" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="21" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="22" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="23" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="24" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="25" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="26" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7993-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="27" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="28" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="29" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature></Features>
  $g<?xml version="1.0"?><Primers nextValidID="12"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL232" sequence="atatCAATTGaattcttatttacctaatctattcatttcaatatcagtatatgcggccGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-07-10T18:07:45Z"><BindingSite location="6760-6842" boundStrand="1" annealedBases="CAATTGaattcttatttacctaatctattcatttcaatatcagtatatgcggccGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="68"><Component bases="atat"/><Component hybridizedRange="6760-6842" bases="CAATTGaattcttatttacctaatctattcatttcaatatcagtatatgcggccGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6760-6842" boundStrand="1" annealedBases="CAATTGaattcttatttacctaatctattcatttcaatatcagtatatgcggccGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="68"><Component bases="atat"/><Component hybridizedRange="6760-6842" bases="CAATTGaattcttatttacctaatctattcatttcaatatcagtatatgcggccGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="1" name="KROL215" sequence="atatCAATTGgtagcttttaaaatgaaaataacaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-27T01:21:36Z"><BindingSite location="6836-6868" boundStrand="0" annealedBases="tCAATTGgtagcttttaaaatgaaaataacaac" meltingTemperature="57"><Component bases="ata"/><Component hybridizedRange="6836-6868" bases="tCAATTGgtagcttttaaaatgaaaataacaac"/></BindingSite><BindingSite simplified="1" location="6836-6868" boundStrand="0" annealedBases="tCAATTGgtagcttttaaaatgaaaataacaac" meltingTemperature="57"><Component bases="ata"/><Component hybridizedRange="6836-6868" bases="tCAATTGgtagcttttaaaatgaaaataacaac"/></BindingSite></Primer><Primer recentID="2" name="KROL214" sequence="atatCAATTGaattcttatttacctaatctattcatt" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T18:54:34Z"><BindingSite location="6810-6842" boundStrand="1" annealedBases="CAATTGaattcttatttacctaatctattcatt" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6810-6842" bases="CAATTGaattcttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="6810-6842" boundStrand="1" annealedBases="CAATTGaattcttatttacctaatctattcatt" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6810-6842" bases="CAATTGaattcttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="3" name="KROL213" sequence="atatGGATCCgaatacttgaacaaagtaaactatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T17:29:11Z"><BindingSite location="6101-6131" boundStrand="0" annealedBases="GGATCCgaatacttgaacaaagtaaactatc" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgaatacttgaacaaagtaaactatc"/></BindingSite><BindingSite simplified="1" location="6101-6131" boundStrand="0" annealedBases="GGATCCgaatacttgaacaaagtaaactatc" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgaatacttgaacaaagtaaactatc"/></BindingSite></Primer><Primer recentID="4" name="KROL216" sequence="atatGGTACCggttatgatatgcttctctacct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-26T19:49:06Z"><BindingSite location="7535-7563" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacct" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7535-7563" bases="GGTACCggttatgatatgcttctctacct"/></BindingSite><BindingSite simplified="1" location="7535-7563" boundStrand="1" annealedBases="GGTACCggttatgatatgcttctctacct" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7535-7563" bases="GGTACCggttatgatatgcttctctacct"/></BindingSite></Primer><Primer recentID="5" name="P792 DFTL_1548FR2Ra" sequence="atatGGTACCtgatactgtagacaacatcatatttaag" description="Contains KpnI site; F. tularensis LVS"><BindingSite location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite location="7509-7518" boundStrand="1" annealedBases="catatttaag" meltingTemperature="20"><Component bases="atatGGTACCtgatactgtagacaacat"/><Component hybridizedRange="7509-7518" bases="catatttaag"/></BindingSite><BindingSite simplified="1" location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component bases="atatGGTACC"/><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7509-7518" boundStrand="1" annealedBases="catatttaag" meltingTemperature="20"><Component bases="atatGGTACCtgatactgtagacaacat"/><Component hybridizedRange="7509-7518" bases="catatttaag"/></BindingSite></Primer><Primer recentID="6" name="P795 CkDFTL_1548R" sequence="tgaattgttgtaataccgccattaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7466-7492" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7466-7492" bases="tgaattgttgtaataccgccattaatc"/></BindingSite><BindingSite simplified="1" location="7466-7492" boundStrand="1" annealedBases="tgaattgttgtaataccgccattaatc" meltingTemperature="56"><Component hybridizedRange="7466-7492" bases="tgaattgttgtaataccgccattaatc"/></BindingSite></Primer><Primer recentID="7" name="P792 DFTL_1548FR2R" sequence="tgatactgtagacaacatcatatttaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite location="7509-7518" boundStrand="1" annealedBases="catatttaag" meltingTemperature="20"><Component bases="tgatactgtagacaacat"/><Component hybridizedRange="7509-7518" bases="catatttaag"/></BindingSite><BindingSite simplified="1" location="7410-7437" boundStrand="1" annealedBases="tgatactgtagacaacatcatatttaag" meltingTemperature="53"><Component hybridizedRange="7410-7437" bases="tgatactgtagacaacatcatatttaag"/></BindingSite><BindingSite simplified="1" location="7509-7518" boundStrand="1" annealedBases="catatttaag" meltingTemperature="20"><Component bases="tgatactgtagacaacat"/><Component hybridizedRange="7509-7518" bases="catatttaag"/></BindingSite></Primer><Primer recentID="8" name="KROL158 FTL1548fragR" sequence="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6760-6798" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6760-6798" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite><BindingSite simplified="1" location="6760-6798" boundStrand="1" annealedBases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg" meltingTemperature="67"><Component hybridizedRange="6760-6798" bases="atatGCGGCCGCtaaaggatcatcatcaaaagttaagcg"/></BindingSite></Primer><Primer recentID="9" name="KROL157 FTL1548fragF" sequence="atatGGTACCaaggcgatttttggatgtatgttaaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6488-6514" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6488-6514" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite><BindingSite simplified="1" location="6488-6514" boundStrand="0" annealedBases="aaggcgatttttggatgtatgttaaag" meltingTemperature="57"><Component bases="atatGGTACC"/><Component hybridizedRange="6488-6514" bases="aaggcgatttttggatgtatgttaaag"/></BindingSite></Primer><Primer recentID="10" name="P813 CompFTL1548_1Ra" sequence="atatGGATCCttataaaggatcatcatcaaaagttaagc" description="Contains BamHI site; F. tularensis LVS"><BindingSite location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="atatGGATCCtta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer><Primer recentID="11" name="P813 CompFTL1548_1R" sequence="ttataaaggatcatcatcaaaagttaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite><BindingSite simplified="1" location="6761-6786" boundStrand="1" annealedBases="taaaggatcatcatcaaaagttaagc" meltingTemperature="54"><Component bases="tta"/><Component hybridizedRange="6761-6786" bases="taaaggatcatcatcaaaagttaagc"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>c7d02ec8-c9bc-4b76-ba6c-d0aa744c94ad</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:28:7">2019.6.26</Created>
<LastModified UTC="18:7:59">2019.7.10</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHO               E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
