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AGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATGTCGATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCATATTTGAATGTATTTAGAAAAATAAACAAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCCAAGCTCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCagttgtttgagttgctatagcaattttagcatcagagagattatatttttttacaagatattgaacctcactacgcgtagatgcttgagtagttgctatgcttataagtaatatagagaatgccgtaaaaaacttagtttttcttttcaaaacctttaaccatataaatttctaaagaatccttaaccgagataatagcattttatctcttataaatacagtaagttatagtatattatctaatcttagatggtagttgtttttaagttaaccctaactaaattactaggcttgcatttatatactaatatgttagaataatccactaattgaaatattggtagagggacattagtccttttaatgtaaacgtaataaactggcatttaagcgaggtgagagggtatgccaagcgttagaattaaagaaagagaaccttttgatgtcgctcttagaagatttaaaagatcttgcgaaaaggctggaatagtatctgaactacgtcgtagagaatactttgaaaagccaacttgggcacgcaaaagaaaaaaaactgccgctgttaaaagagctcataagagcaatataatagttaaaaggtaggcggccgcCTTAACTTTATAAGGAGGAACAGCCATGGCCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAACAATTGttaaatttaattcatcgttgagagtttaactctccctttcacaaggaattcttttttaaaatgtcacaaatttttgatcagttgactcttgatatgaaagagtcaatgaaaaataaagataaaaatagattaacaacaattcgtatggcaat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i)5 Y   .!""##$6%B%&p))*++ S\$i}; "r% e	O7MS\T #&***+%+ }g	(h[ "5%*****+++  8  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                C                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                            	                            
                                                                            
                                                          	                                                                        B                                                          
   	            	         ,                                                                           '                        
                        D   )                        
         %         	               "          
      
      $                     %               	   :   G   A      0   ?   	   -   2   &   "            .         2         ?                                                                                                                                                                         C                           
                                                                                                                                                                                                                                                           
          	            
                            
                      	                          B                    
 	    	   ,                         '        
        D )        
   %   	     "    
  
  $       %     	 : G A  0 ? 	 - 2 & "    .   2   ?             ^  :  
"*< u I	*r)*]{<  .!#)P*b*+"R#t%/&f1 !"%"(M#q
 ^M)*OR@#64u&cccc)&XvS 'OR[dS<; &!u"""@#q##$$$Y%.&&n'w'5(:)f)))o*
#s%B&
W
W,&
WWW 5V!R#t%
&[*I!#%&6<Zc e> DkDD)&w'N])	 
& y>		&	&@E((y	0%( t$(r%E(@)*$
)*	$)
wQ7Xm<" ){3^U!&b&&&( .'ry	"t#C!=(#{"0$,%@*|o
o!#$(z+o
#$z+lB%Z))o
"#$z+X!&(b'	*rK =% .	+&^'!$^ sT%'(I+ruCw,!	"m""-&=()4+ArW	%E &(@)^**5' & 6.*<X!d,}|S\U< /!#;)*P`$++$]EOl0&? 7/##%p+ !%<7X!|!"'$$2&)+8%		 U'o
o!"#$&(z+of&		,%@*0%U=J)4' $QF
yKmqu2EOSDX\0{,fe?   !""#$$$%|'o)))O*t*U< /!
}(	,%'@*,"xh6%0p %"#tk)	m& "&*+!(D( 5'. &'.  
I!!" kOVY\o#%!&&(dn%
}%&(ka((o

FH} D"##$$0%%&(z+ g  f"\cK&			!eUC0"$A%&'|((|))+Z&#{"0$ A*'$EO .
<:Z} "x##$%'&())+lZ$'&() l6.*	(1'[W)e
o1#dwigxb6
!% #zi^ i hp YZ5		8	
XKl$!K5P"$g%%^(((|&T]wI? "##
&;)[***	#		${(	d - 0  	9F&oX[!c""z##7$V%%)))+<'R	?Zo"Fj #$p% >~T	
Y !q""		:elA!%"'~p#'j - 0  	9!c""#7$F&oX[z#V%%)))+LF,~r~m !"%%]	 ""h$%'| -};c>! '!<!$ x )ToNj- 0  !p)r	T
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&T]wI?Y  !q"""##
&;)[***xW
Y<!"#:$$*&c2i

4j'E*jw$) 6 ($hUkc& !!$ } %^!!A#x%%A&x)

i'G ""3" M y-!"$B<T"S+i
O1E^*&x'4 ![%Y" ' 		
+4.PoDZ l!H""$M%%%*a*B~	i
<OT1_E^"*&x'Q(S+DQ ""#y%( 5 tj!"'k))>Ps "t#]%*X*
""I ""#p))X0
:
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name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" 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color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11176" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" 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range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10882-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10507-10526" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10485-10510" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  &<?xml version="1.0"?><Primers nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="13" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10330-10354" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10330-10354" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10330-10354" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10330-10354" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="12" name="KROLXXX pKR37_FR2_F" sequence="ttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9822-9851" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9822-9851" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9822-9851" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9822-9851" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="10" name="KROLXXX pKR37_FR2_R" sequence="atatGGTACCcctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="10418-10452" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10418-10452" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="10418-10452" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10418-10452" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9822" boundStrand="1" annealedBases="aattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atatC"/><Component hybridizedRange="9792-9822" bases="aattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9822" boundStrand="1" annealedBases="aattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atatC"/><Component hybridizedRange="9792-9822" bases="aattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  ?<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:55:0">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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%iXiMQkjYTՑ8N!UDFEfTוB1PDNvmR%UEtfThn^B:`U.ŒD蓝8fFbU] k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    =  &]  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  32<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9822-10446" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="41" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9882-10328" color="#00e600" type="standard"/></Feature><Feature recentID="40" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9882-10328" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10349-10446" color="#00e600" type="standard"/></Feature><Feature recentID="38" name="DNA primase" directionality="1" translationMW="3568.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10468" maxFusedRunOn="10468"><Segment range="10349-10446" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="36" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="29" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="28" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11175" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10881-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10506-10525" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10484-10509" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  &<?xml version="1.0"?><Primers nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="13" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10329-10353" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10329-10353" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10329-10353" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10329-10353" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="12" name="KROLXXX pKR37_FR2_F" sequence="ttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9821-9850" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9821-9850" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9821-9850" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9821-9850" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="10" name="KROLXXX pKR37_FR2_R" sequence="atatGGTACCcctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="10417-10451" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10417-10451" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="10417-10451" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10417-10451" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9822" boundStrand="1" annealedBases="aattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atatC"/><Component hybridizedRange="9792-9822" bases="aattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9822" boundStrand="1" annealedBases="aattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atatC"/><Component hybridizedRange="9792-9822" bases="aattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:51:30">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.V]jZ(D{ubZm({TKXUeFUdiuֲ`n1uSFVtm(%Ua5GQmgmSVD:8PUv=S^VQ
,JC9TFUHUAјMRS9zN
[ITJUNt(b^Ut|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    =  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  32<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-10445" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="41" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9881-10327" color="#00e600" type="standard"/></Feature><Feature recentID="40" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9881-10327" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10348-10445" color="#00e600" type="standard"/></Feature><Feature recentID="38" name="DNA primase" directionality="1" translationMW="3568.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10467" maxFusedRunOn="10467"><Segment range="10348-10445" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="36" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="29" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="28" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11174" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10880-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10505-10524" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10483-10508" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  &<?xml version="1.0"?><Primers nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="13" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10328-10352" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10328-10352" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10328-10352" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10328-10352" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="12" name="KROLXXX pKR37_FR2_F" sequence="ttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9820-9849" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9820-9849" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9820-9849" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9820-9849" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="10" name="KROLXXX pKR37_FR2_R" sequence="atatGGTACCcctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="10416-10450" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10416-10450" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="10416-10450" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10416-10450" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9819" boundStrand="1" annealedBases="tgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atatCaat"/><Component hybridizedRange="9792-9819" bases="tgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9819" boundStrand="1" annealedBases="tgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atatCaat"/><Component hybridizedRange="9792-9819" bases="tgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:51:19">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    =  &[  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  32<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9820-10444" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="41" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9880-10326" color="#00e600" type="standard"/></Feature><Feature recentID="40" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9880-10326" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10347-10444" color="#00e600" type="standard"/></Feature><Feature recentID="38" name="DNA primase" directionality="1" translationMW="3568.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10466" maxFusedRunOn="10466"><Segment range="10347-10444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="36" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="29" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="28" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11173" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10879-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10504-10523" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10482-10507" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  &<?xml version="1.0"?><Primers nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="13" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10327-10351" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10327-10351" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10327-10351" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10327-10351" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="12" name="KROLXXX pKR37_FR2_F" sequence="ttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9819-9848" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9819-9848" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9819-9848" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9819-9848" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="10" name="KROLXXX pKR37_FR2_R" sequence="atatGGTACCcctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="10415-10449" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10415-10449" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="10415-10449" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10415-10449" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9818" boundStrand="1" annealedBases="gTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="59"><Component bases="atatCaatt"/><Component hybridizedRange="9792-9818" bases="gTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9818" boundStrand="1" annealedBases="gTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="59"><Component bases="atatCaatt"/><Component hybridizedRange="9792-9818" bases="gTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:51:14">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VZVJPZV-ժ҇DVTeVF]k&fWU4haUgFBhZuVSTuچv5dNUQYgc5ad9.(D`%[`ԉUQz8$%3![PN1UKdeQ:$N(QUW@'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    =  &Z  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  32<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9819-10443" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="41" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9879-10325" color="#00e600" type="standard"/></Feature><Feature recentID="40" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9879-10325" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10346-10443" color="#00e600" type="standard"/></Feature><Feature recentID="38" name="DNA primase" directionality="1" translationMW="3568.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10465" maxFusedRunOn="10465"><Segment range="10346-10443" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="36" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="29" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="28" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11172" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10878-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10503-10522" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10481-10506" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  &<?xml version="1.0"?><Primers nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="13" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10326-10350" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10326-10350" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10326-10350" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10326-10350" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="12" name="KROLXXX pKR37_FR2_F" sequence="ttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9818-9847" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9818-9847" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9818-9847" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9818-9847" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="10" name="KROLXXX pKR37_FR2_R" sequence="atatGGTACCcctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="10414-10448" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10414-10448" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="10414-10448" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10414-10448" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9817" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="9792-9817" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9817" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="9792-9817" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  ?<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:51:9">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VZVJPZV-ժ҇DVTeVF]k&fWU4haUgFBhZuVSTuچv5dNUQYgc5ad9.(D`%[`ԉUQz8$%3![PN1UKdeQ:$N(QUWCk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    =  &Z  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  32<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9819-10443" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="41" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9879-10325" color="#00e600" type="standard"/></Feature><Feature recentID="40" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9879-10325" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10346-10443" color="#00e600" type="standard"/></Feature><Feature recentID="38" name="DNA primase" directionality="1" translationMW="3568.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10468" maxFusedRunOn="10468"><Segment range="10346-10443" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="36" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="29" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="28" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11175" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10881-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10506-10525" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10484-10509" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  &k<?xml version="1.0"?><Primers nextValidID="14"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="13" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10326-10350" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10326-10350" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10326-10350" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10326-10350" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="12" name="KROLXXX pKR37_FR2_F" sequence="ttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9818-9847" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9818-9847" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9818-9847" boundStrand="0" annealedBases="ttaaatttaattcatcgttgagagtttaac" meltingTemperature="54"><Component hybridizedRange="9818-9847" bases="ttaaatttaattcatcgttgagagtttaac"/></BindingSite></Primer><Primer recentID="10" name="KROLXXX pKR37_FR2_R" sequence="cctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="10414-10442" boundStrand="1" annealedBases="cctgtttttttcaagttaacatatcttga" meltingTemperature="54"><Component hybridizedRange="10414-10442" bases="cctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="10414-10442" boundStrand="1" annealedBases="cctgtttttttcaagttaacatatcttga" meltingTemperature="54"><Component hybridizedRange="10414-10442" bases="cctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9817" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="9792-9817" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9817" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="9792-9817" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:22:45">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     
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#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.V_|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    =   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -<?xml version="1.0"?><Features nextValidID="38"><Feature recentID="37" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="36" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="35" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="34" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="33" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="32" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="31" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="30" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="29" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="28" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-10550" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10256-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9881-9900" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9859-9884" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="10" nextValidID="12"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="9" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="8" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9818" boundStrand="1" annealedBases="gTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="59"><Component bases="atatCaatt"/><Component hybridizedRange="9792-9818" bases="gTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9818" boundStrand="1" annealedBases="gTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="59"><Component bases="atatCaatt"/><Component hybridizedRange="9792-9818" bases="gTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:22:35">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -     _          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcEED_EFPY&y$ETTUU)TNDeBO|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    5   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="26"><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7442" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7148-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6773-6792" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6751-6776" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6681-6709" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6681-6709" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:21:26">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  -     a          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA}5MDiFh_I(:e%cUzJ~ejEejegIJdF	(^VCjfyf)[Z	bW%ڔ!&=LZTTE;QTnUnDYO^EU^<DMfY)T$'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    ?   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="26"><Feature recentID="25" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6720" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6267" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5895.84" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6267" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6523-6720" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6523-6720" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7452" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7158-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6783-6802" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6786" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROLXXX pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6144" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="53"><Component bases="atat"/><Component hybridizedRange="6101-6106" bases="GGATCC"/><Component hybridizedRange="6117-6144" bases="agttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6117-6144" boundStrand="0" annealedBases="agttgtttgagttgctatagcaatttta" meltingTemperature="53"><Component bases="atatGGATCC"/><Component hybridizedRange="6117-6144" bases="agttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="0" name="KROLXXX pKR37_FR1_R" sequence="ctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6691-6719" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6691-6719" bases="ctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="6691-6719" boundStrand="1" annealedBases="ctaccttttaactattatattgctcttat" meltingTemperature="51"><Component hybridizedRange="6691-6719" bases="ctaccttttaactattatattgctcttat"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:20:14">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  !                 .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6839" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="19" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="18" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="17" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="16" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="15" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6545-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="12" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="11" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="8" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="7" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="6" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6170-6189" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="5" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6148-6173" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="4" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="3" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="2" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="1" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="0" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="18:19:59">2019.5.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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l<0=e =,iIDf&Z^8X*X#b3a&AuE";5?w]KyȬs9I|~QWGñ0M:h7?/vQoA`jMT]W.{Kq`CE( R>E\¼!j .%>Hc>IA7 S<UĆkS\6X䷼F!1!V9m@` VfaJ||#)"בU>nA/f`ʁ:Q1eV X`w(A4uf[+KX_IR634N#[7GY Q9I?\;$o6JkJXsǑu?8+Wg?S6a 0jFa9c4cŨ zB츲jfE~a+S~s
 Ը9Zk$1qbYθ+6[({xA{-]h!D8	ph`Uƭ'Y`=j[D>͘wIh.W{/x[HK5'ȻS`zQ
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*,P!<]qx"7{kJso8U];j	rlceaJ;K5N9PY{[ >ZRW}^@ִ*Eݮ{1fv0
|fX\&T9(z#	oT lN;ojB.4vI%")|~`PO=)rh4y22Y*ǃ'9X!`=q8[7_kp,?{nWƏ2A~!JPaXZțF>pl vʝ?UȢk,IT]4q7c!LҫtWU\i-\*3tHu*E,XT^$?H]pFa*Lƃj]$t]:1Wk!_xAZqMCZd֗O,[퐾`^cjyZ{Jjx      脳  I3g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  9<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9825-10449" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9885-10331" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="hypothetical protein" directionality="1" translationMW="16766.68" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9885-10331" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSQIFDQLTLDMKESMKNKDKNRLTTIRMAISAIKQKQIDEKIQITDEVVIAVITKMIKQRQDSFDQYIKANRAELAEGEKREIEILTQYMPKQLNDEEVVAIVQKAIESVGATSMKEMGKIMASVKKELAGRTDMSKVSAIVKSNLA*"/></Q></Feature><Feature recentID="3" name="FTL_1049" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10352-10449" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="DNA primase" directionality="1" translationMW="3568.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="10471" maxFusedRunOn="10471"><Segment range="10352-10449" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAKKVSNSFIKELVATADIVDVVSRYVNLKKT"/></Q></Feature><Feature recentID="5" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6731-6736" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9814-9818" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="7" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="8" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-6713" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="9" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7023-7046" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="10" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7844" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8697-8721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="12" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9341-9365" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="13" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="14" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6744-9818" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6764-6780" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6711-9818" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="17" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6710" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6257" color="#00e600" type="standard"/></Feature><Feature recentID="19" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="5610.58" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6257" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LKRKTKFFTAFSILLISIATTQASTRSEVQYLVKKYNLSDAKIAIATQTT"/></Q></Feature><Feature recentID="20" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6513-6710" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6513-6710" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPSVRIKEREPFDVALRRFKRSCEKAGIVSELRRREYFEKPTWARKRKKTAAVKRAHKSNIIVKR*"/></Q></Feature><Feature recentID="22" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11178" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="24" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="25" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="26" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="27" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="28" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10884-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="29" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="30" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="31" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="32" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="33" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="34" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="35" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="36" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10509-10528" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="37" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10487-10512" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="38" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="39" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="40" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="41" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="42" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features>
  3<?xml version="1.0"?><Primers nextValidID="15"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL148 PCR primer drpsU2 R" sequence="cgccatactgattaaagcttcaacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-29T13:47:43Z"><BindingSite location="10332-10356" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10332-10356" bases="cgccatactgattaaagcttcaacc"/></BindingSite><BindingSite simplified="1" location="10332-10356" boundStrand="1" annealedBases="cgccatactgattaaagcttcaacc" meltingTemperature="59"><Component hybridizedRange="10332-10356" bases="cgccatactgattaaagcttcaacc"/></BindingSite></Primer><Primer recentID="1" name="KROL260 pKR37_FR2_F" sequence="atatCAATTGttaaatttaattcatcgttgagagtttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:10:38Z"><BindingSite location="9818-9853" boundStrand="0" annealedBases="CAATTGttaaatttaattcatcgttgagagtttaac" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="9818-9853" bases="CAATTGttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite location="9847-9857" boundStrand="1" annealedBases="gagagttaac" meltingTemperature="16"><Component bases="atatCAATTGttaaatttaattcatcgtt"/><Component hybridizedRange="9851-9857" bases="gagagtt"/><Component bases="t"/><Component hybridizedRange="9847-9849" bases="aac"/></BindingSite><BindingSite simplified="1" location="9818-9853" boundStrand="0" annealedBases="CAATTGttaaatttaattcatcgttgagagtttaac" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="9818-9853" bases="CAATTGttaaatttaattcatcgttgagagtttaac"/></BindingSite><BindingSite simplified="1" location="9847-9857" boundStrand="1" annealedBases="gagagttaac" meltingTemperature="16"><Component bases="atatCAATTGttaaatttaattcatcgtt"/><Component hybridizedRange="9851-9857" bases="gagagtt"/><Component bases="t"/><Component hybridizedRange="9847-9849" bases="aac"/></BindingSite></Primer><Primer recentID="2" name="KROL261 pKR37_FR2_R" sequence="atatGGTACCcctgtttttttcaagttaacatatcttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:11:47Z"><BindingSite location="7342-7352" boundStrand="1" annealedBases="acatatctga" meltingTemperature="15"><Component bases="atatGGTACCcctgtttttttcaagtta"/><Component hybridizedRange="7346-7352" bases="acatatc"/><Component bases="t"/><Component hybridizedRange="7342-7344" bases="tga"/></BindingSite><BindingSite location="10420-10454" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10420-10454" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite><BindingSite simplified="1" location="7342-7352" boundStrand="1" annealedBases="acatatctga" meltingTemperature="15"><Component bases="atatGGTACCcctgtttttttcaagtta"/><Component hybridizedRange="7346-7352" bases="acatatc"/><Component bases="t"/><Component hybridizedRange="7342-7344" bases="tga"/></BindingSite><BindingSite simplified="1" location="10420-10454" boundStrand="1" annealedBases="GGTACCcctgtttttttcaagttaacatatcttga" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="10420-10454" bases="GGTACCcctgtttttttcaagttaacatatcttga"/></BindingSite></Primer><Primer recentID="3" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6780-6799" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6780-6799" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="4" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6710-6733" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6710-6733" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="5" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9792-9823" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9792-9823" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9792-9823" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9792-9823" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="6" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6983-7004" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6983-7004" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="7" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="7505-7529" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7505-7529" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="8" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8031-8051" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8031-8051" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="9" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8553-8575" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8553-8575" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="10" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9077-9098" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9077-9098" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="11" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9607-9630" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9607-9630" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="12" name="KROL258 pKR37_FR1_F" sequence="atatGGATCCagttgtttgagttgctatagcaatttta" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:05:40Z"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCagttgtttgagttgctatagcaatttta" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCagttgtttgagttgctatagcaatttta"/></BindingSite></Primer><Primer recentID="13" name="KROL259 pKR37_FR1_R" sequence="atatgcggccgcctaccttttaactattatattgctcttat" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-23T20:06:46Z"><BindingSite location="6681-6717" boundStrand="1" annealedBases="gcggccgcctaccttttaactattatattgctcttat" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6681-6717" bases="gcggccgcctaccttttaactattatattgctcttat"/></BindingSite><BindingSite location="10287-10296" boundStrand="1" annealedBases="ttgctctat" meltingTemperature="14"><Component bases="atatgcggccgcctaccttttaactattata"/><Component hybridizedRange="10291-10296" bases="ttgctc"/><Component bases="t"/><Component hybridizedRange="10287-10289" bases="tat"/></BindingSite><BindingSite simplified="1" location="6681-6717" boundStrand="1" annealedBases="gcggccgcctaccttttaactattatattgctcttat" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6681-6717" bases="gcggccgcctaccttttaactattatattgctcttat"/></BindingSite><BindingSite simplified="1" location="10287-10296" boundStrand="1" annealedBases="ttgctctat" meltingTemperature="14"><Component bases="atatgcggccgcctaccttttaactattata"/><Component hybridizedRange="10291-10296" bases="ttgctc"/><Component bases="t"/><Component hybridizedRange="10287-10289" bases="tat"/></BindingSite></Primer><Primer recentID="14" name="KROL175 pKR34_seq_1" sequence="TCTCTCATCCGCCAAAACAGCCAA" dateAdded="2019-10-23T17:20:37Z"><BindingSite location="5882-5905" boundStrand="0" annealedBases="TCTCTCATCCGCCAAAACAGCCAA" meltingTemperature="62"><Component hybridizedRange="5882-5905" bases="TCTCTCATCCGCCAAAACAGCCAA"/></BindingSite><BindingSite location="8382-8405" boundStrand="1" annealedBases="GCCAAAACGCCAA" meltingTemperature="23"><Component bases="TCTCTCATCC"/><Component hybridizedRange="8402-8405" bases="GCCA"/><Component hybridizedRange="8388-8391" bases="AAAC"/><Component bases="A"/><Component hybridizedRange="8382-8386" bases="GCCAA"/></BindingSite><BindingSite simplified="1" location="5882-5905" boundStrand="0" annealedBases="TCTCTCATCCGCCAAAACAGCCAA" meltingTemperature="62"><Component hybridizedRange="5882-5905" bases="TCTCTCATCCGCCAAAACAGCCAA"/></BindingSite><BindingSite simplified="1" location="8382-8391" boundStrand="1" annealedBases="AAACGCCAA" meltingTemperature="23"><Component bases="TCTCTCATCCGCCA"/><Component hybridizedRange="8388-8391" bases="AAAC"/><Component bases="A"/><Component hybridizedRange="8382-8386" bases="GCCAA"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>54d52754-5a9f-4c38-bbfa-373904672a8e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="17:20:37">2019.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                  d     CHO               E              Y? P                                                                                                                                                              )<?xml version="1.0"?><CustomEnzymeSets/>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
