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i)2 V   +!""##$3%?%&X))*n+w+ S\$iz8 "o%&4' e	O7MSYQ #w****+ }g	(hX "2%3't*u*v***k+l+m+  8  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                  @                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                            	                            
                                                                                                                                       	                                                                        @                                                    
      
   	                     *                                                                           $                         
                        D   )                        	         &                                  
            %                     $               	   8   F   B      3   >      *   2   &                .         6         :                                                                                                                                                                          @                           
                                                                                                                                                                                                                                                         
          	            
                                                   	                          @                  
  
 	       *                         $         
        D )        	   &            
    %       $     	 8 F B  3 >  * 2 &      .   6   :      Z          
"*9 u I	*r)*Zx9  +!#)8*J**"O#q%/#c1 !"%"J#q
 ^5)z*OR=#4cccc)XvS 'OR[dS<;v#!r"""=#n##$$$V%+&:(")N)))W*#p%B
T
T)&*(
TTT 5S!O#q%&C*I!#%&39Zc e; DkDA&&NZ)"	L( & y;	&&L(5B'(y	-%( q$(r"~B(()'$(*	$)
wQ4Uj9 ){3^)R&&&Y(( !Q+4(ry	"q#C!x"-$)%(*|o
o!##(b+o
##b+l?%B)})o
"##b+U!&(b	*rH :% .	* !$^ sT%1+ruCw)!"j""*&)+*'ArW	"~B 
&((()F*m*2$ & 6<*<U!d,}|S\R9 ,!##)*]$*+$]EOi-&< 4, #%X+  !%<4U!y!"$$$/&)y+5%		 Ro
o!"##&(b+of&		)%(*-%R=J)4" $QF
yKmqu2EOSDX\-x)cb<   ~!""#$$$%W)t))7*\*R9 ,!

z	)%(*,"xh6%*'-m %"#tS)	m& "&p*+!(D=( !Q2$+ &4(.  
I!!" kOSVYl#%&(dn%

z%&k(o

F
z  A"###$-%%&b+ g  f"\cK&			!eU=O}@-"$>%d)+W&x"-$ A*$EO .
<:Zz "u##$%$&v))|+lZ$$&v) l6<*	\.&7''[T)e
o1 did&xb6!% #zi[ i hp YZ5		8	
XK6 H2M"$d%%'|&T]wI< "##&#)C***	#		$	d - 0  	9F&oUX!`""w##4$S%%x)))~+<'R	?Zo"FMg #$m%( >~T	
V !n""		:i>!""~p#'Mg (- 0  	9!`""#4$F&oUXw#S%%x)))~+LF,~r{j !"%%'']	  ""e$%| -}8`; $!9!$ x )lKg- 0  !p)r	T
vOX9F&o36:|UX  !!"`"""t#w###4$$%S%}%%%x))))T**~+ >~T|	
&T]wI<V  !n"""##&#)C***xW
Y<!"#7$$*&c2i

4j'Egt$) 6 ($hh`#  !!$ } %[!!>#u%%>&`)

i$D !"0" M y-!"$B<T!"';+i
O1B['& X%V" ' 		
+4.MlAW~ i!E""}$J%%%j(*I*B~	i
<OT1!pB["'&&>'';+DQ ""#v%( 5 tg!S))>Ps "q#Z%*X*
""I ""#X))X0
:
0mD#$V%)	 :PLecpK&i''*()*OY6n{R%%}'* ;A	Z
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consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="11"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9789-9820" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9789-9820" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9789-9820" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9789-9820" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatcaattGttatctaaattattactactttgccaatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:15:33Z"><BindingSite location="9815-9850" boundStrand="0" annealedBases="caattGtatctaaattattactactttgccaatc" meltingTemperature="51"><Component bases="atat"/><Component hybridizedRange="9815-9821" bases="caattGt"/><Component bases="t"/><Component hybridizedRange="9824-9850" bases="atctaaattattactactttgccaatc"/></BindingSite><BindingSite simplified="1" location="9824-9850" boundStrand="0" annealedBases="atctaaattattactactttgccaatc" meltingTemperature="51"><Component bases="atatcaattGtt"/><Component hybridizedRange="9824-9850" bases="atctaaattattactactttgccaatc"/></BindingSite></Primer><Primer recentID="9" name="P740 DrpsU1_2F" sequence="atatGCGGCCGCCtttatttaatctaaattattactactttgcc" description="Contains NotI site"><BindingSite location="9823-9846" boundStrand="0" annealedBases="aatctaaattattactactttgcc" meltingTemperature="50"><Component bases="atatGCGGCCGCCtttattt"/><Component hybridizedRange="9823-9846" bases="aatctaaattattactactttgcc"/></BindingSite><BindingSite simplified="1" location="9823-9846" boundStrand="0" annealedBases="aatctaaattattactactttgcc" meltingTemperature="50"><Component bases="atatGCGGCCGCCtttattt"/><Component hybridizedRange="9823-9846" bases="aatctaaattattactactttgcc"/></BindingSite></Primer><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10394-10430" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10394-10430" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10394-10430" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10394-10430" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9883-9910" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9883-9910" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9883-9910" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9883-9910" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10479-10498" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10479-10498" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10479-10498" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10479-10498" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:33:32">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    :  &W  &[  &]  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  7<?xml version="1.0"?><Features nextValidID="38"><Feature recentID="37" name="30S ribosomal protein S21" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="66" maxRunOn="9867" maxFusedRunOn="9867"><Segment range="9822-9824" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="36" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="9822-9824" color="#00e600" type="standard"/></Feature><Feature recentID="35" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="172" hitsStopCodon="1"><Segment range="9822-9824" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="34" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9822-9824" color="#00e600" type="standard"/></Feature><Feature recentID="33" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9822-9824" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10451-10467" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="26" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10859-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10425-10429" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11153" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9806" maxFusedRunOn="9806"><Segment range="9825-10336" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9825-10336" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9825-10424" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9817-9821" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
   L<?xml version="1.0"?><Primers nextValidID="11"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9789-9820" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9789-9820" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9789-9820" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9789-9820" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatcaattGttatctaaattattactactttgccaatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:15:33Z"><BindingSite location="9815-9849" boundStrand="0" annealedBases="caattGttatctaaattattactactttgccaatc" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="9815-9849" bases="caattGttatctaaattattactactttgccaatc"/></BindingSite><BindingSite simplified="1" location="9815-9849" boundStrand="0" annealedBases="caattGttatctaaattattactactttgccaatc" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="9815-9849" bases="caattGttatctaaattattactactttgccaatc"/></BindingSite></Primer><Primer recentID="9" name="P740 DrpsU1_2F" sequence="atatGCGGCCGCCtttatttaatctaaattattactactttgcc" description="Contains NotI site"><BindingSite location="9823-9845" boundStrand="0" annealedBases="atctaaattattactactttgcc" meltingTemperature="49"><Component bases="atatGCGGCCGCCtttattta"/><Component hybridizedRange="9823-9845" bases="atctaaattattactactttgcc"/></BindingSite><BindingSite simplified="1" location="9823-9845" boundStrand="0" annealedBases="atctaaattattactactttgcc" meltingTemperature="49"><Component bases="atatGCGGCCGCCtttattta"/><Component hybridizedRange="9823-9845" bases="atctaaattattactactttgcc"/></BindingSite></Primer><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10393-10429" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10393-10429" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10393-10429" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10393-10429" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9882-9909" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9882-9909" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9882-9909" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9882-9909" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10478-10497" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10478-10497" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10478-10497" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10478-10497" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:32:46">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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{OIkӄ9J-Uhi()nVeziYW玪1qVoU]-qjU6[i:ziFj6Uhj[gY,__R꩕FZUV/fwmn8fdZٽxyy$֚fUQRU8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    :  &W  &Z  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="172" hitsStopCodon="1"><Segment range="9821-9823" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="34" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-9823" color="#00e600" type="standard"/></Feature><Feature recentID="33" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-9823" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10450-10466" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="26" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10858-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10424-10428" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11152" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9619" maxFusedRunOn="9619"><Segment range="9824-10335" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9824-10335" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9824-10423" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9816-9820" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
  ;<?xml version="1.0"?><Primers nextValidID="10"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="9" name="P740 DrpsU1_2F" sequence="atatGCGGCCGCCtttatttaatctaaattattactactttgcc" description="Contains NotI site"><BindingSite location="9822-9844" boundStrand="0" annealedBases="atctaaattattactactttgcc" meltingTemperature="49"><Component bases="atatGCGGCCGCCtttattta"/><Component hybridizedRange="9822-9844" bases="atctaaattattactactttgcc"/></BindingSite><BindingSite simplified="1" location="9822-9844" boundStrand="0" annealedBases="atctaaattattactactttgcc" meltingTemperature="49"><Component bases="atatGCGGCCGCCtttattta"/><Component hybridizedRange="9822-9844" bases="atctaaattattactactttgcc"/></BindingSite></Primer><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10392-10428" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10392-10428" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10392-10428" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10392-10428" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9820-9851" boundStrand="0" annealedBases="tttctaaattattactactttgccaatcttt" meltingTemperature="52"><Component bases="atatCaa"/><Component hybridizedRange="9820-9821" bases="tt"/><Component bases="G"/><Component hybridizedRange="9823-9851" bases="tctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9820-9851" boundStrand="0" annealedBases="tttctaaattattactactttgccaatcttt" meltingTemperature="52"><Component bases="atatCaa"/><Component hybridizedRange="9820-9821" bases="tt"/><Component bases="G"/><Component hybridizedRange="9823-9851" bases="tctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9881-9908" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9881-9908" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9881-9908" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" 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hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:14:24">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    :  &W  &Z  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="26" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10855-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10421-10425" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11149" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9619" maxFusedRunOn="9619"><Segment range="9821-10332" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-10332" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-10420" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9816-9820" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
  4<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10389-10425" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10389-10425" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10389-10425" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10389-10425" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9815-9848" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="9815-9848" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9815-9848" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="9815-9848" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9878-9905" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9878-9905" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9878-9905" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9878-9905" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10474-10493" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10474-10493" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10474-10493" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10474-10493" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:9:25">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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{OIkӄ9J-UhZi()nVeziYW玪1qVoU]-qjU6[i:ziFj6Uhj[gY,__R꩕FZUV/fwmn8fdZٽxyy$֚fUQRU8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    :  &W  &Z  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature 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name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10858-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10424-10428" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11152" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9619" maxFusedRunOn="9619"><Segment range="9824-10335" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9824-10335" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9824-10423" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9816-9820" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
  `<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10392-10428" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10392-10428" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10392-10428" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10392-10428" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9815-9851" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="51"><Component bases="atatC"/><Component hybridizedRange="9815-9819" bases="aattG"/><Component hybridizedRange="9823-9851" bases="tctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9823-9851" boundStrand="0" annealedBases="tctaaattattactactttgccaatcttt" meltingTemperature="51"><Component bases="atatCaattG"/><Component hybridizedRange="9823-9851" bases="tctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9881-9908" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9881-9908" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9881-9908" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9881-9908" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10477-10496" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10477-10496" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10477-10496" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10477-10496" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:4:55">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    :  &W  &Z  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature 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name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10855-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10421-10425" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11149" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9619" maxFusedRunOn="9619"><Segment range="9821-10332" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-10332" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9821-10420" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9816-9820" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
  4<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10389-10425" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10389-10425" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10389-10425" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10389-10425" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9815-9848" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="9815-9848" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9815-9848" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="9815-9848" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9878-9905" boundStrand="1" 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boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:4:45">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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{OIkӄ9J-Uhi()nVeziYW玪1qVoU]-qjU6[i:ziFj6Uhj[gY,__R꩕FZUV/fwmn8fdZٽxyy$֚fUQRU8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    :  &W  &Z  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="26" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10858-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10424-10428" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11152" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9619" maxFusedRunOn="9619"><Segment range="9824-10335" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9824-10335" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9824-10423" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9816-9820" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
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dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10392-10428" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10392-10428" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10392-10428" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10392-10428" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9820-9851" boundStrand="0" annealedBases="tttctaaattattactactttgccaatcttt" meltingTemperature="52"><Component bases="atatCaa"/><Component hybridizedRange="9820-9821" bases="tt"/><Component bases="G"/><Component hybridizedRange="9823-9851" bases="tctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9820-9851" boundStrand="0" annealedBases="tttctaaattattactactttgccaatcttt" 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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:4:36">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    :  &W  &Z  &\  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature></Features>
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dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10389-10425" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10389-10425" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10389-10425" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10389-10425" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9815-9848" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="9815-9848" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9815-9848" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="9815-9848" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9878-9905" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9878-9905" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9878-9905" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9878-9905" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10474-10493" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10474-10493" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10474-10493" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10474-10493" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:4:20">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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[^5aU;9㪌\UvWv\ZMN^QMbei~ԺeQd֨}f;Gjm(K٫@e[jY$DjiVo~*i^^FI5eaTeN	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    6  8     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="20" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8041" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7313-7317" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7747-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7339-7355" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="0" nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6706" boundStrand="1" annealedBases="ttaaataaatgctcttctctgagaaattc" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6678-6706" bases="ttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6706" boundStrand="1" annealedBases="ttaaataaatgctcttctctgagaaattc" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6678-6706" bases="ttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="7281-7317" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="7281-7317" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="7281-7317" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="7281-7317" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="6707-6740" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="6707-6740" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="6707-6740" boundStrand="0" annealedBases="aattGtctaaattattactactttgccaatcttt" meltingTemperature="57"><Component bases="atatC"/><Component hybridizedRange="6707-6740" bases="aattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="6770-6797" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="6770-6797" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="6770-6797" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="6770-6797" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7366-7385" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7366-7385" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7366-7385" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7366-7385" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="13:38:25">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Rd     
  +        +.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"֋z[YYe^N:ZWmUwlũTn}=YV&_6mg}}KUFMjkWmVj[ۓ~b9f[UKiP5DFkf⦕dZiVVEIVZk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    2  &I  &L  &N  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="20" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11135" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10407-10411" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10841-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10433-10449" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6730-9801" color="#993366" type="standard"/><Q name="label"><V text="lacZ"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7014-7037" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7806-7835" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8688-8712" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9332-9356" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="1" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6730-9801" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="0" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6722-6729" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature></Features>
  q<?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL169 pKR34_lacZ_F" sequence="atatGCGGCCGCAGGAGGAGGAGGAACAGCCATGGCCATGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:25:05Z"><BindingSite location="6707-6743" boundStrand="0" annealedBases="GCGGCCGCAGGAGGAGGAGGAACAGCCATGGCCATGA" meltingTemperature="76"><Component bases="atat"/><Component hybridizedRange="6707-6743" bases="GCGGCCGCAGGAGGAGGAGGAACAGCCATGGCCATGA"/></BindingSite><BindingSite simplified="1" location="6707-6743" boundStrand="0" annealedBases="GCGGCCGCAGGAGGAGGAGGAACAGCCATGGCCATGA" meltingTemperature="76"><Component bases="atat"/><Component hybridizedRange="6707-6743" bases="GCGGCCGCAGGAGGAGGAGGAACAGCCATGGCCATGA"/></BindingSite></Primer><Primer recentID="1" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6715" boundStrand="1" annealedBases="tGCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="66"><Component bases="ata"/><Component hybridizedRange="6678-6715" bases="tGCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6715" boundStrand="1" annealedBases="tGCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="66"><Component bases="ata"/><Component hybridizedRange="6678-6715" bases="tGCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="2" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10375-10411" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10375-10411" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10375-10411" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10375-10411" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="3" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9799-9834" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9799-9834" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9799-9834" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9799-9834" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="5" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9864-9891" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9864-9891" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9864-9891" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9864-9891" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="6" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="7" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10460-10479" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10460-10479" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10460-10479" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10460-10479" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="8" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6771-6790" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6771-6790" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6771-6790" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6771-6790" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="13:38:16">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"֋z[YYe^N:ZWmUwlũTn}=YV&_6mg}}KUFMjkWmVj[ۓ~b9f[UKiP5DFkf⦕dZiVVEIVZk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    2  &A  &D  &F  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  +6<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="10" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6714-6721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="30" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6722-9793" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="29" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9324-9348" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="9" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8680-8704" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7798-7827" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7006-7029" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="6" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6722-9793" color="#993366" type="standard"/><Q name="label"><V text="lacZ"/></Q></Feature><Feature recentID="28" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10425-10441" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="22" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10833-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10399-10403" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11127" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9793" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9792" maxFusedRunOn="9792"><Segment range="9799-10310" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9799-10310" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9799-10398" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9794-9798" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
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meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="8" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10452-10471" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10452-10471" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10452-10471" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10452-10471" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="9" 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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:19:21">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"֋z[YYe^N:ZWmUwlũTn}=YV&_6mg}}KUFMjkWmVj[ۓ~b9f[UKiP5DFkf⦕dZiVVEIVZk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    2  &9  &<  &>  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10825-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10391-10395" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11119" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9785" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9784" maxFusedRunOn="9784"><Segment range="9791-10302" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9791-10302" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9791-10390" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9786-9790" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,3" nextValidID="10"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10359-10395" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10359-10395" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10359-10395" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10359-10395" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="1" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9783-9818" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9783-9818" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9783-9818" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9783-9818" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL168 pKR34_FR1_R" sequence="atatCCATGGCCTCCTttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:05:07Z"><BindingSite location="6678-6713" boundStrand="1" annealedBases="GCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="62"><Component bases="atatCCATG"/><Component hybridizedRange="6678-6713" bases="GCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6713" boundStrand="1" annealedBases="GCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="62"><Component bases="atatCCATG"/><Component hybridizedRange="6678-6713" bases="GCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="5" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="6" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9848-9875" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9848-9875" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9848-9875" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9848-9875" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="7" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="8" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10444-10463" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10444-10463" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10444-10463" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10444-10463" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6755-6774" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6755-6774" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6755-6774" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6755-6774" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:18:31">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="22" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10827-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10393-10397" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11121" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9787" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9786" maxFusedRunOn="9786"><Segment range="9793-10304" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9793-10304" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9793-10392" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9788-9792" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
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description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9785-9820" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9785-9820" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9785-9820" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9785-9820" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL168 pKR34_FR1_R" sequence="atatCCATGGCCTCCTttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:05:07Z"><BindingSite location="6678-6713" boundStrand="1" annealedBases="GCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="62"><Component bases="atatCCATG"/><Component hybridizedRange="6678-6713" bases="GCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6713" boundStrand="1" annealedBases="GCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="62"><Component bases="atatCCATG"/><Component hybridizedRange="6678-6713" bases="GCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="5" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" 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description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6755-6774" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6755-6774" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6755-6774" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6755-6774" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:17:44">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    2  ;  >  @     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7755-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7321-7325" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8049" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6714-6715" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-6715" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6714" maxFusedRunOn="6714"><Segment range="6721-7232" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6721-7232" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6721-7320" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6716-6720" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
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description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="6713-6748" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="6713-6748" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="6713-6748" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="6713-6748" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="4" name="KROL168 pKR34_FR1_R" sequence="atatCCATGGCCTCCTttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:05:07Z"><BindingSite location="6678-6715" boundStrand="1" annealedBases="TGCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="60"><Component 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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:17:28">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="22" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10825-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10391-10395" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11119" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6725-9785" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6998-7021" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7790-7819" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8672-8696" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9316-9340" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9785" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9784" maxFusedRunOn="9784"><Segment range="9791-10302" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9791-10302" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9791-10390" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9786-9790" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:16:26">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  )O<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10414-10430" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="22" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10822-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10388-10392" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11116" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6722-9782" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6995-7018" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7787-7816" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8669-8693" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9313-9337" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9782" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9781" maxFusedRunOn="9781"><Segment range="9788-10299" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9788-10299" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9788-10387" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9783-9787" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="10"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10356-10392" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10356-10392" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10356-10392" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10356-10392" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="1" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9780-9815" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9780-9815" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9780-9815" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9780-9815" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="2" name="KROL170 pKR34_lacZ_R" sequence="atatCaattGACACCAGACCAACTGGTAATGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:14:21Z"><BindingSite location="9758-9786" boundStrand="1" annealedBases="CaattGACACCAGACCAACTGGTAATGGT" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="9758-9786" bases="CaattGACACCAGACCAACTGGTAATGGT"/></BindingSite><BindingSite simplified="1" location="9758-9786" boundStrand="1" annealedBases="CaattGACACCAGACCAACTGGTAATGGT" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="9758-9786" bases="CaattGACACCAGACCAACTGGTAATGGT"/></BindingSite></Primer><Primer recentID="3" name="KROL169 pKR34_lacZ_F" sequence="atatAGGAGGCCATGGCCATGATTACGGATTCAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:10:09Z"><BindingSite location="6707-6736" boundStrand="0" annealedBases="AGGAGGCCATGGCCATGATTACGGATTCAC" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="6707-6736" bases="AGGAGGCCATGGCCATGATTACGGATTCAC"/></BindingSite><BindingSite location="526-535" boundStrand="1" annealedBases="ACGGATTCAC" meltingTemperature="35"><Component bases="atatAGGAGGCCATGGCCATGATT"/><Component hybridizedRange="526-535" bases="ACGGATTCAC"/></BindingSite><BindingSite simplified="1" location="6707-6736" boundStrand="0" annealedBases="AGGAGGCCATGGCCATGATTACGGATTCAC" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="6707-6736" bases="AGGAGGCCATGGCCATGATTACGGATTCAC"/></BindingSite><BindingSite simplified="1" location="526-535" boundStrand="1" annealedBases="ACGGATTCAC" meltingTemperature="35"><Component bases="atatAGGAGGCCATGGCCATGATT"/><Component hybridizedRange="526-535" bases="ACGGATTCAC"/></BindingSite></Primer><Primer recentID="4" name="KROL168 pKR34_FR1_R" sequence="atatCCATGGCCTCCTttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:05:07Z"><BindingSite location="6678-6718" boundStrand="1" annealedBases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6678-6718" bases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6718" boundStrand="1" annealedBases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6678-6718" bases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="5" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="6" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9845-9872" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9845-9872" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9845-9872" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9845-9872" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="7" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="8" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10441-10460" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10441-10460" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10441-10460" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10441-10460" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6752-6771" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6752-6771" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6752-6771" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6752-6771" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:13:35">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"֋z[YYe^N:ZWmUwlũTn}=YV&_6mg}}KUFMjkWmVj[ۓ~b9f[UKiP5DFkf⦕dZiVVEIVZk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    2  &9  &<  &>  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  )O<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10417-10433" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="22" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10825-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10391-10395" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11119" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6725-9785" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6998-7021" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7790-7819" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8672-8696" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9316-9340" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9785" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9784" maxFusedRunOn="9784"><Segment range="9791-10302" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9791-10302" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9791-10390" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9786-9790" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  0<?xml version="1.0"?><Primers nextValidID="10"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10359-10395" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10359-10395" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10359-10395" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10359-10395" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="1" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9783-9818" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9783-9818" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9783-9818" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9783-9818" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="2" name="KROL170 pKR34_lacZ_R" sequence="atatCaattGACACCAGACCAACTGGTAATGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:14:21Z"><BindingSite location="9761-9789" boundStrand="1" annealedBases="CaattGACACCAGACCAACTGGTAATGGT" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="9761-9789" bases="CaattGACACCAGACCAACTGGTAATGGT"/></BindingSite><BindingSite simplified="1" location="9761-9789" boundStrand="1" annealedBases="CaattGACACCAGACCAACTGGTAATGGT" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="9761-9789" bases="CaattGACACCAGACCAACTGGTAATGGT"/></BindingSite></Primer><Primer recentID="3" name="KROL169 pKR34_lacZ_F" sequence="atatAGGAGGCCATGGCCATGATTACGGATTCAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:10:09Z"><BindingSite location="6707-6739" boundStrand="0" annealedBases="AGGAGGCCATGGCCATGATTACGGATTCAC" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6712" bases="AGGAGG"/><Component hybridizedRange="6716-6739" bases="CCATGGCCATGATTACGGATTCAC"/></BindingSite><BindingSite location="526-535" boundStrand="1" annealedBases="ACGGATTCAC" meltingTemperature="35"><Component bases="atatAGGAGGCCATGGCCATGATT"/><Component hybridizedRange="526-535" 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bases="CCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6712" boundStrand="1" annealedBases="CCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="59"><Component bases="atatCCATGG"/><Component hybridizedRange="6678-6712" bases="CCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="5" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component 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bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="8" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10444-10463" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10444-10463" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10444-10463" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10444-10463" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6755-6774" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6755-6774" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6755-6774" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6755-6774" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:13:30">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  )O<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10414-10430" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="22" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="18" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10822-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="17" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10388-10392" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11116" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="15" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="10" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6722-9782" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6995-7018" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="8" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7787-7816" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8669-8693" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9313-9337" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="5" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9782" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="4" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9781" maxFusedRunOn="9781"><Segment range="9788-10299" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9788-10299" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9788-10387" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9783-9787" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="10"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10356-10392" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10356-10392" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10356-10392" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10356-10392" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="1" name="KROL171 pKR34_FR2_F" sequence="atatCaattGtctaaattattactactttgccaatcttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:17:51Z"><BindingSite location="9780-9815" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9780-9815" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite><BindingSite simplified="1" location="9780-9815" boundStrand="0" annealedBases="tCaattGtctaaattattactactttgccaatcttt" meltingTemperature="58"><Component bases="ata"/><Component hybridizedRange="9780-9815" bases="tCaattGtctaaattattactactttgccaatcttt"/></BindingSite></Primer><Primer recentID="2" name="KROL170 pKR34_lacZ_R" sequence="atatCaattGACACCAGACCAACTGGTAATGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:14:21Z"><BindingSite location="9758-9786" boundStrand="1" annealedBases="CaattGACACCAGACCAACTGGTAATGGT" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="9758-9786" bases="CaattGACACCAGACCAACTGGTAATGGT"/></BindingSite><BindingSite simplified="1" location="9758-9786" boundStrand="1" annealedBases="CaattGACACCAGACCAACTGGTAATGGT" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="9758-9786" bases="CaattGACACCAGACCAACTGGTAATGGT"/></BindingSite></Primer><Primer recentID="3" name="KROL169 pKR34_lacZ_F" sequence="atatAGGAGGCCATGGCCATGATTACGGATTCAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:10:09Z"><BindingSite location="6707-6736" boundStrand="0" annealedBases="AGGAGGCCATGGCCATGATTACGGATTCAC" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="6707-6736" bases="AGGAGGCCATGGCCATGATTACGGATTCAC"/></BindingSite><BindingSite location="526-535" boundStrand="1" annealedBases="ACGGATTCAC" meltingTemperature="35"><Component bases="atatAGGAGGCCATGGCCATGATT"/><Component hybridizedRange="526-535" bases="ACGGATTCAC"/></BindingSite><BindingSite simplified="1" location="6707-6736" boundStrand="0" annealedBases="AGGAGGCCATGGCCATGATTACGGATTCAC" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="6707-6736" bases="AGGAGGCCATGGCCATGATTACGGATTCAC"/></BindingSite><BindingSite simplified="1" location="526-535" boundStrand="1" annealedBases="ACGGATTCAC" meltingTemperature="35"><Component bases="atatAGGAGGCCATGGCCATGATT"/><Component hybridizedRange="526-535" bases="ACGGATTCAC"/></BindingSite></Primer><Primer recentID="4" name="KROL168 pKR34_FR1_R" sequence="atatCCATGGCCTCCTttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:05:07Z"><BindingSite location="6678-6718" boundStrand="1" annealedBases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6678-6718" bases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6718" boundStrand="1" annealedBases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6678-6718" bases="CCATGGCCTCCTttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="5" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="6" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9845-9872" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9845-9872" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9845-9872" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9845-9872" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="7" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="8" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10441-10460" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10441-10460" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10441-10460" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10441-10460" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6752-6771" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6752-6771" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6752-6771" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6752-6771" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="0:12:45">2019.5.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  ,<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="hypothetical protein" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="303" maxRunOn="9797" maxFusedRunOn="9797"><Segment range="9788-9791" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="30" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="9788-9791" color="#00e600" type="standard"/></Feature><Feature recentID="29" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9788-9791" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9788-9792" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="27" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9793-10392" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="4" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9793-10304" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9786" maxFusedRunOn="9786"><Segment range="9793-10304" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6713" color="#a6acb3" type="standard"/></Feature><Feature recentID="25" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9787" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="24" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9318-9342" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8674-8698" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7792-7821" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7000-7023" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="20" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6727-9787" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11121" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10393-10397" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10827-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10419-10435" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:1:32">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    2  &G  &J  &L  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="hypothetical protein" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="303" maxRunOn="9809" maxFusedRunOn="9809"><Segment range="9800-9803" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="30" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="9800-9803" color="#00e600" type="standard"/></Feature><Feature recentID="29" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9800-9803" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9800-9804" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="27" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9805-10404" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="4" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9805-10316" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9798" maxFusedRunOn="9798"><Segment range="9805-10316" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6720-6725" color="#a6acb3" type="standard"/></Feature><Feature recentID="25" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9799" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="24" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9330-9354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8686-8710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7804-7833" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7012-7035" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="20" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6739-9799" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11133" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10405-10409" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10839-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10431-10447" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="1,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="P740 DrpsU1_2F" sequence="atatGCGGCCGCCtttatttaatctaaattattactactttgcc" description="Contains NotI site"><BindingSite location="9804-9825" boundStrand="0" annealedBases="tctaaattattactactttgcc" meltingTemperature="48"><Component bases="atatGCGGCCGCCtttatttaa"/><Component hybridizedRange="9804-9825" bases="tctaaattattactactttgcc"/></BindingSite><BindingSite simplified="1" location="9804-9825" boundStrand="0" annealedBases="tctaaattattactactttgcc" meltingTemperature="48"><Component bases="atatGCGGCCGCCtttatttaa"/><Component hybridizedRange="9804-9825" bases="tctaaattattactactttgcc"/></BindingSite></Primer><Primer recentID="3" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9862-9889" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9862-9889" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9862-9889" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9862-9889" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="4" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10458-10477" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10458-10477" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10458-10477" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10458-10477" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6769-6788" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6769-6788" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6769-6788" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6769-6788" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:47:22">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	    2  &G  &K  &M  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="hypothetical protein" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="302" maxRunOn="9810" maxFusedRunOn="9810"><Segment range="9800-9804" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="30" name="FTL_0702" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="9800-9804" color="#00e600" type="standard"/></Feature><Feature recentID="29" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9800-9804" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="28" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9800-9805" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="27" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9806-10405" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="4" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9806-10317" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9739" maxFusedRunOn="9739"><Segment range="9806-10317" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6720-6725" color="#a6acb3" type="standard"/></Feature><Feature recentID="25" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9799" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="24" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9330-9354" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8686-8710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7804-7833" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7012-7035" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="20" name="lacZ" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6739-9799" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="lacZ"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="2" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11134" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10406-10410" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10840-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10432-10448" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="1,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="P740 DrpsU1_2F" sequence="atatGCGGCCGCCtttatttaatctaaattattactactttgcc" description="Contains NotI site"><BindingSite location="9805-9826" boundStrand="0" annealedBases="tctaaattattactactttgcc" meltingTemperature="48"><Component bases="atatGCGGCCGCCtttatttaa"/><Component hybridizedRange="9805-9826" bases="tctaaattattactactttgcc"/></BindingSite><BindingSite simplified="1" location="9805-9826" boundStrand="0" annealedBases="tctaaattattactactttgcc" meltingTemperature="48"><Component bases="atatGCGGCCGCCtttatttaa"/><Component hybridizedRange="9805-9826" bases="tctaaattattactactttgcc"/></BindingSite></Primer><Primer recentID="3" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9863-9890" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9863-9890" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9863-9890" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9863-9890" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="4" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="10459-10478" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10459-10478" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10459-10478" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10459-10478" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6769-6788" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6769-6788" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6769-6788" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6769-6788" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:35:43">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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{OIkӄ9J.i()nVeziYW玪1qVoU]-qjU6[i:ziFj6Uhj[gY,__R꩕FZUV/fwmn8fdZٽxyy$֚fUQRU8'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    2  &G  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11128" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10400-10404" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10834-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10426-10442" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
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  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:35:18">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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{OIkӄ9J,'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    2   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9826-9842" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:27:7">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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UQٵZVWy8PԧE	BSY	X&$a7Wچe*Q^r:1U8&N7=tUQQUSUQjDښ'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    2   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6734-6750" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  }<?xml version="1.0"?><Primers recycledIDs="3,2,1,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="4" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6761-6780" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="6761-6780" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6761-6780" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="6761-6780" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:19:7">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  $                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features recycledIDs="0,1,6,2,3,4,5" nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6836" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6134-6150" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="4,3,2,1,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:15:50">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  (               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAB|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	       -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="2,3,4,5" nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6850" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6122-6126" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6556-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6148-6164" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6121" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6121" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="hypothetical protein" directionality="1" translationMW="316.36" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="146" hitsStopCodon="1"><Segment range="6108-6121" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
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  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:12:7">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ;~   
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	           -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7184-7200" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7157" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6368" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6042" maxFusedRunOn="6042"><Segment range="6108-6368" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6624-6650" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="30S ribosomal protein S21" directionality="1" translationMW="831.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6624-6650" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6711-7157" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="hypothetical protein" directionality="1" translationMW="16767.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6711-7157" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  5<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL123 ∆rpsU2 FR2R" sequence="atatGGTACCttaagctaagttagatttcacaatagcg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7129-7162" boundStrand="1" annealedBases="GGTACCttaagctaagttagatttcacaatagcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7129-7162" bases="GGTACCttaagctaagttagatttcacaatagcg"/></BindingSite><BindingSite simplified="1" location="7129-7162" boundStrand="1" annealedBases="GGTACCttaagctaagttagatttcacaatagcg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="7129-7162" bases="GGTACCttaagctaagttagatttcacaatagcg"/></BindingSite></Primer><Primer recentID="1" name="KROL122 ∆rpsU2 FR1F" sequence="atatGGATCCaaaattactaggtatcgtaaaaagtgcc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6134" boundStrand="0" annealedBases="GGATCCaaaattactaggtatcgtaaaaagtgcc" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCaaaattactaggtatcgtaaaaagtgcc"/></BindingSite><BindingSite simplified="1" location="6101-6134" boundStrand="0" annealedBases="GGATCCaaaattactaggtatcgtaaaaagtgcc" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6134" bases="GGATCCaaaattactaggtatcgtaaaaagtgcc"/></BindingSite></Primer><Primer recentID="2" name="KROL121 ∆rpsU2 seq primer F" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6530-6558" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="52"><Component hybridizedRange="6530-6558" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="6530-6558" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="52"><Component hybridizedRange="6530-6558" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="3" name="KROL119 ∆rpsU2 FR2F" sequence="atatGCGGCCGCTaaaaggtagttaaatttaattcatcgttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6632-6670" boundStrand="0" annealedBases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6632-6670" bases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga"/></BindingSite><BindingSite simplified="1" location="6632-6670" boundStrand="0" annealedBases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6632-6670" bases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga"/></BindingSite></Primer><Primer recentID="4" name="KROL120 ∆rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6609-6639" boundStrand="1" annealedBases="GCGGCCGCgcttggcataccctctcacctcg" meltingTemperature="74"><Component bases="atat"/><Component hybridizedRange="6609-6639" bases="GCGGCCGCgcttggcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="6609-6639" boundStrand="1" annealedBases="GCGGCCGCgcttggcataccctctcacctcg" meltingTemperature="74"><Component bases="atat"/><Component hybridizedRange="6609-6639" bases="GCGGCCGCgcttggcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7211-7230" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7211-7230" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7211-7230" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7211-7230" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="22:11:57">2019.5.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAU%T5Ui'-d6ei(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBc<PklUujaQ-aUUZɃYUMUYѷZ8UFMYBTDdVh{YMyXaD(+EdKlaQ	V5"UT^	Fa5ILV:Pk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="hypothetical protein" directionality="1" translationMW="16767.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6712-7158" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6712-7158" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="831.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6625-6651" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="16" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6625-6651" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6369" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6369" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7158" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7185-7201" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="2" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7593-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="1" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7159-7163" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7887" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature></Features>
  y<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL123 ∆rpsU2 FR2R" sequence="ttaagctaagttagatttcacaatagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7130-7157" boundStrand="1" annealedBases="ttaagctaagttagatttcacaatagcg" meltingTemperature="55"><Component hybridizedRange="7130-7157" bases="ttaagctaagttagatttcacaatagcg"/></BindingSite><BindingSite simplified="1" location="7130-7157" boundStrand="1" annealedBases="ttaagctaagttagatttcacaatagcg" meltingTemperature="55"><Component hybridizedRange="7130-7157" bases="ttaagctaagttagatttcacaatagcg"/></BindingSite></Primer><Primer recentID="1" name="KROL122 ∆rpsU2 FR1F" sequence="taaaattactaggtatcgtaaaaagtgc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6134" boundStrand="0" annealedBases="taaaattactaggtatcgtaaaaagtgc" meltingTemperature="53"><Component hybridizedRange="6107-6134" bases="taaaattactaggtatcgtaaaaagtgc"/></BindingSite><BindingSite simplified="1" location="6107-6134" boundStrand="0" annealedBases="taaaattactaggtatcgtaaaaagtgc" meltingTemperature="53"><Component hybridizedRange="6107-6134" bases="taaaattactaggtatcgtaaaaagtgc"/></BindingSite></Primer><Primer recentID="2" name="KROL121 ∆rpsU2 seq primer F" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6531-6559" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="52"><Component hybridizedRange="6531-6559" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="6531-6559" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="52"><Component hybridizedRange="6531-6559" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="3" name="KROL119 ∆rpsU2 FR2F" sequence="atatGCGGCCGCTaaaaggtagttaaatttaattcatcgttga" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6633-6671" boundStrand="0" annealedBases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6633-6671" bases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga"/></BindingSite><BindingSite simplified="1" location="6633-6671" boundStrand="0" annealedBases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6633-6671" bases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttga"/></BindingSite></Primer><Primer recentID="4" name="KROL120 ∆rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6610-6640" boundStrand="1" annealedBases="GCGGCCGCgcttggcataccctctcacctcg" meltingTemperature="74"><Component bases="atat"/><Component hybridizedRange="6610-6640" bases="GCGGCCGCgcttggcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="6610-6640" boundStrand="1" annealedBases="GCGGCCGCgcttggcataccctctcacctcg" meltingTemperature="74"><Component bases="atat"/><Component hybridizedRange="6610-6640" bases="GCGGCCGCgcttggcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7212-7231" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7212-7231" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7212-7231" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7212-7231" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:41:42">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .)               ƛ.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAU%T5Ui'-d6ei(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcBJVJPZV-ժ҇DVTeVF]k&fWU4haUgFBhZuVSTuچv5dNUQYgc5ad9.(D`%[`ԉUQz8$%3![B|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="7,8,9,11,12" nextValidID="25"><Feature recentID="6" name="hypothetical protein" directionality="1" translationMW="16767.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6703-7149" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6703-7149" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="617.77" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6625-6642" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6625-6642" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6369" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6369" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7149" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7176-7192" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7584-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7150-7154" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7878" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7222" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7203-7222" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7203-7222" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7203-7222" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:10:3">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .W       z        z.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAU%T5Ui'-d6ei(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcEED_EFPY&y$ETTUU)TNDeBJVJPZV-ժ҇DVTeVF]k&fWU4haUgFBhZuVSTuچv5dNUQYgc5ad9.(D`%[`ԉUQz8$%3![B|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="7,8,9,11,12" nextValidID="25"><Feature recentID="6" name="hypothetical protein" directionality="1" translationMW="16767.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6883-7329" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6883-7329" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7840.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6625-6822" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6625-6822" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6369" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6369" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7329" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7356-7372" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7764-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7330-7334" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8058" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7383-7402" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7383-7402" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7383-7402" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7383-7402" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:9:25">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  $p                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2<?xml version="1.0"?><Features recycledIDs="0,1,2,3,4,5,6,7,8,9,11,12" nextValidID="25"><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6134-6150" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6836" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:5:0">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  B       B        B.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAW~ZjJUnڹhV;aU
^YYh0Z[>ީ.ZUi*+Ӛn:vz)OJ	TcbTkzuZ9_~kLvwPRmkN5I5im9S'JiMXj^jKE^IbFni3ij剕%|juN|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	        h  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7044-7060" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7452-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7018-7022" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6293-6297" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q></Feature><Feature recentID="11" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6306" maxFusedRunOn="6306"><Segment range="6293-6297" color="#993366" type="standard" translated="1"/><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7746" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="9" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6302-7017" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6302-6310" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="proton-dependent oligopeptide transport" directionality="1" translationMW="246.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="512" hitsStopCodon="1"><Segment range="6302-6310" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="6" name="FTL_1252" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-7017" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="ATP-dependent RNA helicase RhlE" directionality="2" translationMW="25921.40" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="206" hitsStopCodon="1"><Segment range="6307-7017" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="proton-dependent oligopeptide transport" directionality="1" translationMW="331.43" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6310" maxFusedRunOn="6310"><Segment range="6284-6292" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6284-6292" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="transposase" directionality="1" translationMW="3618.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="193" hitsStopCodon="1"><Segment range="6108-6197" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6197" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6292" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  `<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL18 DFTL1251_FR1Fa" sequence="atatGGATCCtttatttggctactgagaaactatttttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6135" boundStrand="0" annealedBases="GGATCCtttatttggctactgagaaactatttttg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6135" bases="GGATCCtttatttggctactgagaaactatttttg"/></BindingSite><BindingSite simplified="1" location="6101-6135" boundStrand="0" annealedBases="GGATCCtttatttggctactgagaaactatttttg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6135" bases="GGATCCtttatttggctactgagaaactatttttg"/></BindingSite></Primer><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7071-7090" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7071-7090" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7071-7090" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7071-7090" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="3" name="KROL3 DFTL1251_FR2F" sequence="atatGCGGCCGCCaataattaatcttttgcctttggttttct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6291-6329" boundStrand="0" annealedBases="tGCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="6291-6329" bases="tGCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite><BindingSite simplified="1" location="6291-6329" boundStrand="0" annealedBases="tGCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="6291-6329" bases="tGCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite></Primer><Primer recentID="4" name="KROL4 DFTL1251_FR2R" sequence="atatGGTACCgtatcagctgatgttgtaaatacgac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6991-7022" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6991-7022" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite><BindingSite simplified="1" location="6991-7022" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6991-7022" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite></Primer><Primer recentID="5" name="KROL1 DFTL1251_FR1F" sequence="atatGGATCCttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6158-6188" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component bases="atatGGATCC"/><Component hybridizedRange="6158-6188" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6158-6188" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component bases="atatGGATCC"/><Component hybridizedRange="6158-6188" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="6" name="KROL2 DFTL1251_FR1R" sequence="atatGCGGCCGCaatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6262-6299" boundStrand="1" annealedBases="GCGGCCGCaatagtcacaattattctcctattttaaac" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6262-6299" bases="GCGGCCGCaatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6262-6299" boundStrand="1" annealedBases="GCGGCCGCaatagtcacaattattctcctattttaaac" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6262-6299" bases="GCGGCCGCaatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:4:37">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ?               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<ꏨ*wj&mE
(4曥ljN"d'iS뒾UmbǭޝDVW=+demF5ZfMyGjfF[NTIҡ}y=6Wzi!LZbejIn,vӆpk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  j  5  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="ATP-dependent RNA helicase RhlE" directionality="2" translationMW="25921.40" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="206" hitsStopCodon="1"><Segment range="6256-6966" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="23" name="FTL_1252" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6256-6966" color="#00e600" type="standard"/></Feature><Feature recentID="22" name="proton-dependent oligopeptide transport" directionality="1" translationMW="246.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="512" hitsStopCodon="1"><Segment range="6251-6259" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="21" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6251-6259" color="#00e600" type="standard"/></Feature><Feature recentID="20" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6251-6966" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="proton-dependent oligopeptide transport" directionality="1" translationMW="331.43" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6259" maxFusedRunOn="6259"><Segment range="6233-6241" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6233-6241" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="transposase" directionality="1" translationMW="1461.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="210" hitsStopCodon="1"><Segment range="6108-6146" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6146" color="#00e600" type="standard"/></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6241" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="14" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7695" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6255" maxFusedRunOn="6255"><Segment range="6242-6246" color="#993366" type="standard" translated="1"/><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" 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name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6993-7009" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
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  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:10:19">2018.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  1       C        C.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6146" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6241" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6979" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" 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name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6685-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6277-6293" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL1 DFTL1251_FR1F" sequence="ttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="0" name="KROL2 DFTL1251_FR1R" sequence="aatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:35:2">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ;       ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<:hkYz]Tj:U{ZUnkfiTjJDQNNIj=hrH`iQffiښWݚdڄ iUUE[ign֡%6YYi[SYFid(dij數暊*)Vd]WZaayYZeRjYVeVijtiiiV&Yi?[9VmugkoVI[jVfʎԋ7o$e@k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  j  a  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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int="1"/></Q></Feature><Feature recentID="18" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6146" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6241" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7995" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6345" maxFusedRunOn="6345"><Segment range="6242-6246" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6242-6246" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7050" maxFusedRunOn="7050"><Segment range="7081-7271" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7081-7271" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6345" maxFusedRunOn="6345"><Segment range="6256-6264" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6256-6264" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6536" maxFusedRunOn="6536"><Segment range="6560-6835" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6560-6835" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7701-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7293-7309" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL1 DFTL1251_FR1F" sequence="ttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="0" name="KROL2 DFTL1251_FR1R" sequence="aatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:34:17">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  1                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAϣ	XUFnYV)jiifJ^En։g*DMnhiv}e}٦II%X	UZd[hyZ?Fz
j[]QYnjEEU5JDfIbHZibnVIYUۍzoIUNVuU&fZ5ijjGJVeijfY=^Xv}fdeflHq&BJYji]4	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &?<?xml version="1.0"?><Features recycledIDs="17,18" nextValidID="22"><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7861" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6211" maxFusedRunOn="6211"><Segment range="6108-6112" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6916" maxFusedRunOn="6916"><Segment range="6947-7137" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6947-7137" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6211" maxFusedRunOn="6211"><Segment range="6122-6130" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6122-6130" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6402" maxFusedRunOn="6402"><Segment range="6426-6701" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6426-6701" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7159-7175" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:34:13">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  6        "        ".	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAbfV9jefId)jfbeIilߖV*_J^Yx4mM2JRfD}rM%MĩnjnQO6mZ7זUzwYHdfJH$c֪٨jQmhA4gXeJ%ͦ>NWWYIeRFjE6Jg^ڢ%qJaiNa^NvvV_c*Ine.ꑎej훦 &&Zh'j,QUdYa)]deX9>&-cV^UNf^֦[ZjٚDU)zSZOZ%+i6Yif&&j`&UimhjAh꛸*uEmuFIfMmZjVyTy)Y%"ZnڪjyUY%eWn6ꩽ%V:eXkEVTUjdեZje*ZcEZiIZOg]dyaՒaڕ"ě	+e=;Yt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="17" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        ѮZTR
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  ><?xml version="1.0"?><Features nextValidID="37"><Feature recentID="0" name="Potential RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6733" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9811-9815" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="2" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp4"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6708-6710" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="JCOL782 (3' end)"/></Q></Feature><Feature recentID="4" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7020-7043" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="5" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7841" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="6" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8694-8718" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="7" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9338-9362" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="8" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6708-9815" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="9" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6741-9815" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK*"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6761-6777" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9817-9821" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9826-10425" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="13" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9826-10337" color="#00e600" type="standard"/></Feature><Feature recentID="14" name="acetyltransferase" directionality="2" translationMW="20277.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="9822" maxFusedRunOn="9822"><Segment range="9826-10337" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNFNKSLYYCDISLQIMTQNDFDRLYSVAKDPEIWTQHNDKSRSELDGFRKYFDGGLNNPQNCYLIFYKKDLVGSTRYYEYDSNAKSIKIGYTFYAKKYWGTDLNGKVKKLMVDYAFKFVDDVFFDVWDKNFRSQKAVAKLGAKFYCHNIAQQKFIFRLTNKDWQSSNNL"/></Q></Feature><Feature recentID="15" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6707" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="16" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6510-6707" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="30S ribosomal protein S21" directionality="1" translationMW="7847.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6510-6707" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MLSIRVDEHKPFDISLRNFKRACEKAGIKQELRDRQHYVKPTEKRKIAKRQAVKRARISQRRAFI*"/></Q></Feature><Feature recentID="18" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-6510" color="#00e600" type="standard"/></Feature><Feature recentID="19" name="cold shock protein" directionality="1" translationMW="7385.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6307-6510" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNNKSQGTVKFFNEQKGFGFITPENGGKDVFVHISKLNGETLAEGQQVTFETQEGRKGPEAINIEVL*"/></Q></Feature><Feature recentID="20" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-11154" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="10426-10430" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="22" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10860-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="23" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="24" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="25" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="26" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="28" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10452-10468" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9822-9825" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="34" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9822-9825" color="#00e600" type="standard"/></Feature><Feature recentID="35" name="rpsU1" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="9822-9825" color="#00e600" type="standard"/></Feature><Feature recentID="36" name="30S ribosomal protein S21" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="66" hitsStopCodon="1"><Segment range="9822-9825" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="*"/></Q></Feature></Features>
  H<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL204 pKR34_seq_new" sequence="ccatacaccaataactagctttaatag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-06-14T16:35:58Z"><BindingSite location="6245-6271" boundStrand="0" annealedBases="ccatacaccaataactagctttaatag" meltingTemperature="55"><Component hybridizedRange="6245-6271" bases="ccatacaccaataactagctttaatag"/></BindingSite><BindingSite simplified="1" location="6245-6271" boundStrand="0" annealedBases="ccatacaccaataactagctttaatag" meltingTemperature="55"><Component hybridizedRange="6245-6271" bases="ccatacaccaataactagctttaatag"/></BindingSite></Primer><Primer recentID="1" name="KROL183 pKR34_seq_9" sequence="tttataaaagattagatagcaattttgcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:33:57Z"><BindingSite location="10130-10158" boundStrand="0" annealedBases="tttataaaagattagatagcaattttgcg" meltingTemperature="54"><Component hybridizedRange="10130-10158" bases="tttataaaagattagatagcaattttgcg"/></BindingSite><BindingSite simplified="1" location="10130-10158" boundStrand="0" annealedBases="tttataaaagattagatagcaattttgcg" meltingTemperature="54"><Component hybridizedRange="10130-10158" bases="tttataaaagattagatagcaattttgcg"/></BindingSite></Primer><Primer recentID="2" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="9604-9627" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9604-9627" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="9604-9627" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="9604-9627" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="3" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="9074-9095" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9074-9095" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="9074-9095" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="9074-9095" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="4" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="8550-8572" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8550-8572" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="8550-8572" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="8550-8572" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="5" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="8028-8048" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8028-8048" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="8028-8048" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="8028-8048" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="6" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="7502-7526" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7502-7526" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite location="10019-10033" boundStrand="1" annealedBases="ACAGTCTTATGGC" meltingTemperature="22"><Component bases="CTACGGGTA"/><Component hybridizedRange="10030-10033" bases="ACAG"/><Component bases="TT"/><Component hybridizedRange="10025-10028" bases="TCTT"/><Component bases="T"/><Component hybridizedRange="10019-10023" bases="ATGGC"/></BindingSite><BindingSite simplified="1" location="7502-7526" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="7502-7526" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="10019-10033" boundStrand="1" annealedBases="ACAGTCTTATGGC" meltingTemperature="22"><Component bases="CTACGGGTA"/><Component hybridizedRange="10030-10033" bases="ACAG"/><Component bases="TT"/><Component hybridizedRange="10025-10028" bases="TCTT"/><Component bases="T"/><Component hybridizedRange="10019-10023" bases="ATGGC"/></BindingSite></Primer><Primer recentID="7" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="6980-7001" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6980-7001" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="6980-7001" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="6980-7001" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="8" name="KROL176 pKR34_seq_2" sequence="tggaaaagatgtatttgtacacatatca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:18:32Z"><BindingSite location="6383-6410" boundStrand="0" annealedBases="tggaaaagatgtatttgtacacatatca" meltingTemperature="55"><Component hybridizedRange="6383-6410" bases="tggaaaagatgtatttgtacacatatca"/></BindingSite><BindingSite location="7399-7409" boundStrand="0" annealedBases="tacacaatca" meltingTemperature="11"><Component bases="tggaaaagatgtatttg"/><Component hybridizedRange="7399-7404" bases="tacaca"/><Component bases="t"/><Component hybridizedRange="7406-7409" bases="atca"/></BindingSite><BindingSite simplified="1" location="6383-6410" boundStrand="0" annealedBases="tggaaaagatgtatttgtacacatatca" meltingTemperature="55"><Component hybridizedRange="6383-6410" bases="tggaaaagatgtatttgtacacatatca"/></BindingSite><BindingSite simplified="1" location="7399-7409" boundStrand="0" annealedBases="tacacaatca" meltingTemperature="11"><Component bases="tggaaaagatgtatttg"/><Component hybridizedRange="7399-7404" bases="tacaca"/><Component bases="t"/><Component hybridizedRange="7406-7409" bases="atca"/></BindingSite></Primer><Primer recentID="9" name="KROL175 pKR24_seq_1" sequence="TCTCTCATCCGCCAAAACAGCCAA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:16:13Z"><BindingSite location="5882-5905" boundStrand="0" annealedBases="TCTCTCATCCGCCAAAACAGCCAA" meltingTemperature="62"><Component hybridizedRange="5882-5905" bases="TCTCTCATCCGCCAAAACAGCCAA"/></BindingSite><BindingSite location="8379-8402" boundStrand="1" annealedBases="GCCAAAACGCCAA" meltingTemperature="23"><Component bases="TCTCTCATCC"/><Component hybridizedRange="8399-8402" bases="GCCA"/><Component hybridizedRange="8385-8388" bases="AAAC"/><Component bases="A"/><Component hybridizedRange="8379-8383" bases="GCCAA"/></BindingSite><BindingSite simplified="1" location="5882-5905" boundStrand="0" annealedBases="TCTCTCATCCGCCAAAACAGCCAA" meltingTemperature="62"><Component hybridizedRange="5882-5905" bases="TCTCTCATCCGCCAAAACAGCCAA"/></BindingSite><BindingSite simplified="1" location="8379-8388" boundStrand="1" annealedBases="AAACGCCAA" meltingTemperature="23"><Component bases="TCTCTCATCCGCCA"/><Component hybridizedRange="8385-8388" bases="AAAC"/><Component bases="A"/><Component hybridizedRange="8379-8383" bases="GCCAA"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite location="9789-9820" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9789-9820" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="2080-2089" boundStrand="0" annealedBases="ACCAACTGGT" meltingTemperature="37"><Component bases="atatCaattgTTATTTTTGACACCAG"/><Component hybridizedRange="2080-2089" bases="ACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="9789-9820" boundStrand="1" annealedBases="CaattgTTATTTTTGACACCAGACCAACTGGT" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="9789-9820" bases="CaattgTTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL171 pKR34_FR2_F" sequence="atatcaattGttaatctaaattattactactttgccaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:37:29Z"><BindingSite location="9815-9850" boundStrand="0" annealedBases="caattGttaatctaaattattactactttgccaatc" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="9815-9850" bases="caattGttaatctaaattattactactttgccaatc"/></BindingSite><BindingSite simplified="1" location="9815-9850" boundStrand="0" annealedBases="caattGttaatctaaattattactactttgccaatc" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="9815-9850" bases="caattGttaatctaaattattactactttgccaatc"/></BindingSite></Primer><Primer recentID="12" name="P740 DrpsU1_2F" sequence="atatGCGGCCGCCtttatttaatctaaattattactactttgcc" description="Contains NotI site"><BindingSite location="9821-9846" boundStrand="0" annealedBases="ttaatctaaattattactactttgcc" meltingTemperature="50"><Component bases="atatGCGGCCGCCtttat"/><Component hybridizedRange="9821-9846" bases="ttaatctaaattattactactttgcc"/></BindingSite><BindingSite simplified="1" location="9821-9846" boundStrand="0" annealedBases="ttaatctaaattattactactttgcc" meltingTemperature="50"><Component bases="atatGCGGCCGCCtttat"/><Component hybridizedRange="9821-9846" bases="ttaatctaaattattactactttgcc"/></BindingSite></Primer><Primer recentID="13" name="KROL169 pKR34_lacZ_F" sequence="atatgcggccgcCTTAACTTTATAAGGA" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T13:41:50Z"><BindingSite location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite><BindingSite simplified="1" location="6707-6730" boundStrand="0" annealedBases="gcggccgcCTTAACTTTATAAGGA" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6707-6730" bases="gcggccgcCTTAACTTTATAAGGA"/></BindingSite></Primer><Primer recentID="14" name="KROL168 pKR34_FR1_R" sequence="atatGCGGCCGCttaaataaatgctcttctctgagaaattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T00:22:59Z"><BindingSite location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite><BindingSite simplified="1" location="6678-6714" boundStrand="1" annealedBases="GCGGCCGCttaaataaatgctcttctctgagaaattc" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6678-6714" bases="GCGGCCGCttaaataaatgctcttctctgagaaattc"/></BindingSite></Primer><Primer recentID="15" name="KROL172 pKR34_FR2_R" sequence="atatGGTACCagctaaaattattttactttcttatcattta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T23:20:27Z"><BindingSite location="10394-10430" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10394-10430" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite><BindingSite simplified="1" location="10394-10430" boundStrand="1" annealedBases="GGTACCagctaaaattattttactttcttatcattta" meltingTemperature="56"><Component bases="atat"/><Component hybridizedRange="10394-10430" bases="GGTACCagctaaaattattttactttcttatcattta"/></BindingSite></Primer><Primer recentID="16" name="KROL167 pKR34_FR1_F" sequence="atatGGATCCcagttacatatatcttttagtaatattttata" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-20T22:57:35Z"><BindingSite location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite><BindingSite simplified="1" location="6101-6138" boundStrand="0" annealedBases="GGATCCcagttacatatatcttttagtaatattttata" meltingTemperature="54"><Component bases="atat"/><Component hybridizedRange="6101-6138" bases="GGATCCcagttacatatatcttttagtaatattttata"/></BindingSite></Primer><Primer recentID="17" name="P742 CkDrpsU1_F" sequence="gctaagttttattgtcataatattgctc" description="0"><BindingSite location="9883-9910" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9883-9910" bases="gctaagttttattgtcataatattgctc"/></BindingSite><BindingSite simplified="1" location="9883-9910" boundStrand="1" annealedBases="gctaagttttattgtcataatattgctc" meltingTemperature="53"><Component hybridizedRange="9883-9910" bases="gctaagttttattgtcataatattgctc"/></BindingSite></Primer><Primer recentID="18" name="P739 DrpsU1_1F" sequence="atatGCGGCCGCacttagcattatagtacctcaatattga" description="Contains NotI site"><BindingSite location="6490-6499" boundStrand="0" annealedBases="tcaatattga" meltingTemperature="17"><Component bases="atatGCGGCCGCacttagcattatagtacc"/><Component hybridizedRange="6490-6499" bases="tcaatattga"/></BindingSite><BindingSite location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6499" boundStrand="0" annealedBases="tcaatattga" meltingTemperature="17"><Component bases="atatGCGGCCGCacttagcattatagtacc"/><Component hybridizedRange="6490-6499" bases="tcaatattga"/></BindingSite><BindingSite simplified="1" location="6490-6517" boundStrand="1" annealedBases="acttagcattatagtacctcaatattga" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="6490-6517" bases="acttagcattatagtacctcaatattga"/></BindingSite></Primer><Primer recentID="19" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite location="10479-10498" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10479-10498" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite location="10729-10739" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="10734-10739" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="10729-10732" bases="GATA"/></BindingSite><BindingSite simplified="1" location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10479-10498" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="10479-10498" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="10729-10739" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="10734-10739" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="10729-10732" bases="GATA"/></BindingSite></Primer><Primer recentID="20" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6777-6796" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6777-6796" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-02-21T14:22:59Z"><BindingSite location="9790-9814" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGG" meltingTemperature="57"><Component bases="atatggatcC"/><Component hybridizedRange="9790-9814" bases="TTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="9790-9814" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGG" meltingTemperature="57"><Component bases="atatggatcC"/><Component hybridizedRange="9790-9814" bases="TTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:23:0">2020.2.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d     CHR  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
