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K}v!   1kK6i	


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 m_qT A%	Mm;	##vN72 v3#;U	I	Q		r]6\4[y#(Ht;\, 6|4Y
_BeK#FW3 {;I}x
9YiQM&QT!`f*m JU\c&,A
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a BRq(			f$8! R <	

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5k7w(F kR		M t5z)31 ?w4$rGVcJ		v
	s*!]!&Icu5]sv) V<PE	4	:`^G\$-/< b0	mz7-}Ud]X! 
ICZ	 	c


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-|"}# ?QVtlCsVy = `  
'u	
>o$
/i  dy.<^03N	

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Ici#
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!ot+DL^N\^t{7 @~5LH|w

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		5	?		
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r
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:$		9;%fY&B;g  AUZ	/NC_  +D}O){k%J	Zf" %\K
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  
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69\ 3PVq   |)K
ExP7 '.nw1	


0J"El,)^*7<; \id}  |)g? 
(^EHJLl Ulm	vYZ,2# BRo;%	f<T9RLP   =9		:gXJC: C[	&Q '.<h   %?-U^	
FPkT $Bi	p *e	D V 
y*j(  "L*sJ[	%P0t :O$M`#  P|} z&K@T]rTw%Y  !gbn ,E~LTF}	t
l4e,~rOXdk
 ' 4  s*s	j[g# ]VLv	N/SNI=&>I[ u v-lr	~BT)!  &f	

/!]+(6ck T<	P8XI1  >#~ITh		
i)#	 S\$i e	O7MS%0Hj }g	(h#$  -x  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                            +                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                   +               	                                                                      !                                                                                
   
                  	                  	            )      	            	                                             
                                                      (   (   
   $   "   
      $         {   k      !   ,         5   _                                                                                                                                                                       +                                                                                                                                                                                                                                                                                                      
                                                                                                       +     	                          !                              
 
      	      	    )  	    	               
                  ( ( 
 $ " 
  $   { k  ! ,   5 _           )    k
bku I	*r)*bkM"/1 :#q
^OR4ccccXvS 'OR[dS<;tBB
, 5I&Zc5 eDkDN
	 y	[(y	( LrO*	@
wQ{3^" ry	C|o
oo
lo
b	2r.	V$^ sTzruCwyArW	8 6<d,}|S\%S6$$]EOh<.		o
oof&		=J4 $QF
yKmqu2EOSDX\>
	,"xh6t	m_D 8.  
I kO"dn
k(o

F g  f"\cK&			!eU7n AC$EO .
<:Z$lZ l6	[;[-e
o1LRdisxb6 #zi|&T]wI&	#		$	d- 0  	9F&o&8<'R	?Zo"F >~T	
		:~p'- 0  	9F&o&8LF,~rXd]	| -} x )- 0  !p)r	T
vOX9F&o&8;L >~T|	
&T]wI&xW
Y<t&c2i

4j'E) 6 ($h } %

il M y-B<Ti
O1 ' 		
+4./V_B~	i
<OT1ExDQ ( 5 t>PsX*
I#X0
:
0mD	 :PLelOY6! ;A	Z
C f]kG6:  k	V(i@  74Z{>'G K	 	:PLel!p)rT
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="19" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="18" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="17" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="16" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="15" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6148-6173" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="14" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6170-6189" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="13" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="12" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="11" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="9" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="8" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="7" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="6" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6545-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="5" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="4" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="3" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="2" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="1" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6839" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>a79c3c1f-0a39-46d9-ad19-9ee932989227</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="4:0:0">2018.7.21</Created>
<LastModified UTC="4:0:0">2018.7.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  P                fjs~oCTU&$gId穡]Em5}TY5`XiTU&[eQftef:BvTedfie'z.UFZ}UegiΚ)UwSjzF0f          
         -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="4"><Feature recentID="3" name="lipoprotein" directionality="2" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="373" hitsStopCodon="1"><Segment range="513-518" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_1096" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="513-518" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="FTL_1095" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-246" color="#0080ff" type="standard"/><Q name="note"><V text="bicyclomycin resistance protein"/></Q></Feature><Feature recentID="0" name="FTL_1095" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="11-246" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="dFTL_1096FR1_R" sequence="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:57:14Z"><BindingSite location="483-527" boundStrand="1" annealedBases="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg" meltingTemperature="61"><Component hybridizedRange="483-527" bases="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg"/></BindingSite><BindingSite simplified="1" location="483-527" boundStrand="1" annealedBases="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg" meltingTemperature="61"><Component hybridizedRange="483-527" bases="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg"/></BindingSite></Primer><Primer recentID="1" name="dFTL_1096_FR1_F" sequence="atatGGATCCgtgaggattttcttacgctcaaacc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:57:14Z"><BindingSite location="0-34" boundStrand="0" annealedBases="atatGGATCCgtgaggattttcttacgctcaaacc" meltingTemperature="64"><Component hybridizedRange="0-34" bases="atatGGATCCgtgaggattttcttacgctcaaacc"/></BindingSite><BindingSite simplified="1" location="0-34" boundStrand="0" annealedBases="atatGGATCCgtgaggattttcttacgctcaaacc" meltingTemperature="64"><Component hybridizedRange="0-34" bases="atatGGATCCgtgaggattttcttacgctcaaacc"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>b2ff2459-87a3-4910-9d9e-43553899a796</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:57:24">2019.3.26</Created>
<LastModified UTC="0:57:24">2019.3.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
          O        Of0-V髪Ojk[hڣj)*hg۪G_j)Yoj
Xh}tYyv۠1]E%MגVDJZe%]\*emꛛR*ilmF;y(iUJSf`Ǎdj齍6B}&            D  K  N   -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="6"><Feature recentID="5" name="FTL_1098" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="420-582" color="#0080ff" type="standard"/><Q name="note"><V text="4Fe-4S ferredoxin (electron transport) family protein, pseudogene"/></Q></Feature><Feature recentID="4" name="FTL_1098" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="420-582" color="#00e600" type="standard"/></Feature><Feature recentID="3" name="macrophage infectivity potentiator" directionality="2" translationMW="10524.32" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="42-332" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="FTL_1097" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="42-332" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="lipoprotein" directionality="2" translationMW="378.49" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1" maxFusedRunOn="1"><Segment range="13-21" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="FTL_1096" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="13-21" color="#00e600" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="dFTL_1096_FR2_F" sequence="atatGCGGCCGCcttagtcattctaaatttactcctttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:58:08Z"><BindingSite location="0-40" boundStrand="0" annealedBases="atatGCGGCCGCcttagtcattctaaatttactcctttaac" meltingTemperature="65"><Component hybridizedRange="0-40" bases="atatGCGGCCGCcttagtcattctaaatttactcctttaac"/></BindingSite><BindingSite simplified="1" location="0-40" boundStrand="0" annealedBases="atatGCGGCCGCcttagtcattctaaatttactcctttaac" meltingTemperature="65"><Component hybridizedRange="0-40" bases="atatGCGGCCGCcttagtcattctaaatttactcctttaac"/></BindingSite></Primer><Primer recentID="1" name="dFTL_1096_FR2_R" sequence="atatGGTACCtatgctgcatggataagaaatatcgct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:58:09Z"><BindingSite location="554-590" boundStrand="1" annealedBases="atatGGTACCtatgctgcatggataagaaatatcgct" meltingTemperature="63"><Component hybridizedRange="554-590" bases="atatGGTACCtatgctgcatggataagaaatatcgct"/></BindingSite><BindingSite simplified="1" location="554-590" boundStrand="1" annealedBases="atatGGTACCtatgctgcatggataagaaatatcgct" meltingTemperature="63"><Component hybridizedRange="554-590" bases="atatGGTACCtatgctgcatggataagaaatatcgct"/></BindingSite></Primer></Primers>
  h<Notes>
<UUID>d7005a11-eae7-47e4-80c8-a0bce09fb14c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:58:27">2019.3.26</Created>
<LastModified UTC="0:58:27">2019.3.26</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  +z<?xml version="1.0"?><HistoryTree><Node name="pKR31 pEX_dFTL_1096.dna" type="DNA" seqLen="7919" ID="5" circular="1" operation="insertFragments"><RegeneratedSite name="BamHI" pos="6102" siteCount="1"/><RegeneratedSite name="NotI" pos="6615" siteCount="1"/><RegeneratedSite name="KpnI" pos="7195" siteCount="1"/><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="6102..6614" colors="red"/><ColorRange range="6615..7194" colors="blue"/></TopStrand><BottomStrand><ColorRange range="6106..6618" colors="red"/><ColorRange range="6619..7190" colors="blue"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" name1="KpnI" name2="BamHI" val1="6115" val2="6102" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="BamHI" name2="NotI" val1="5" val2="518" siteCount1="1" siteCount2="1"/><InputSummary manipulation="insert" name1="NotI" name2="KpnI" val1="6" val2="586" siteCount1="1" siteCount2="1"/><Node name="pEX.gbk" type="DNA" seqLen="6839" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6545-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature name="promoter" type="promoter" allowSegmentOverlaps="0"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6148-6173" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="6102..6114" colors="white"/></TopStrand><BottomStrand><ColorRange range="6106..6110" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node><Node name="FR1.dna" type="DNA" seqLen="528" ID="2" circular="0" upstreamModification="Unmodified" downstreamModification="Unmodified" resurrectable="1" operation="amplifyFragment"><Features><Feature name="FTL_1095" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="11-246" color="#0080ff" type="standard"/><Q name="note"><V text="bicyclomycin resistance protein"/></Q></Feature><Feature name="FTL_1096" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="513-518" color="#00e600" type="standard"/></Feature></Features><Oligo name="dFTL_1096_FR1_F" sequence="atatGGATCCgtgaggattttcttacgctcaaacc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"/><Oligo name="dFTL_1096FR1_R" sequence="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"/><Parameter name="polymerase" val="polymerase that creates blunt ends"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="5..517" colors="red"/><ColorRange range="0..4" colors="white"/><ColorRange range="518..527" colors="white"/></TopStrand><BottomStrand><ColorRange range="9..521" colors="red"/><ColorRange range="0..8" colors="white"/><ColorRange range="522..527" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="0..9" colors="red"/><ColorRange range="518..527" colors="red"/></TopStrand><BottomStrand><ColorRange range="0..9" colors="red"/><ColorRange range="518..527" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="amplify" val1="1043312" val2="1043819"/><Node name="LVSgenomeWithPrimers_JW.dna" type="DNA" seqLen="1895994" ID="1" circular="1" operation="invalid"><Primers><HybridizationParams minContinuousMatchLen="15" allowMismatch="0" minMeltingTemperature="40"/><Primer recentID="0" name="dFTL_1096_FR1_F" sequence="atatGGATCCgtgaggattttcttacgctcaaacc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1043312-1043336" boundStrand="0" annealedBases="gtgaggattttcttacgctcaaacc" meltingTemperature="58"><Component bases="atatGGATCC"/><Component hybridizedRange="1043312-1043336" bases="gtgaggattttcttacgctcaaacc"/></BindingSite></Primer><Primer recentID="1" name="dFTL_1096FR1_R" sequence="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1043785-1043819" boundStrand="1" annealedBases="GCTtaattagattaatctataaaagtttaaaaatg" meltingTemperature="52"><Component bases="atatGCGGCC"/><Component hybridizedRange="1043785-1043819" bases="GCTtaattagattaatctataaaagtttaaaaatg"/></BindingSite></Primer></Primers><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1043312..1043819" colors="black"/><ColorRange range="1043820..1043311" colors="white"/></TopStrand><BottomStrand><ColorRange range="1043312..1043819" colors="black"/><ColorRange range="1043820..1043311" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node><Node name="FR2.dna" type="DNA" seqLen="591" ID="4" circular="0" upstreamModification="Unmodified" downstreamModification="Unmodified" resurrectable="1" operation="amplifyFragment"><Features><Feature name="FTL_1097" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="42-332" color="#00e600" type="standard"/></Feature><Feature name="FTL_1098" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="420-582" color="#0080ff" type="standard"/><Q name="note"><V text="4Fe-4S ferredoxin (electron transport) family protein, pseudogene"/></Q></Feature><Feature name="FTL_1096" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="13-21" color="#00e600" type="standard"/></Feature></Features><Oligo name="dFTL_1096_FR2_F" sequence="atatGCGGCCGCcttagtcattctaaatttactcctttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"/><Oligo name="dFTL_1096_FR2_R" sequence="atatGGTACCtatgctgcatggataagaaatatcgct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"/><Parameter name="polymerase" val="polymerase that creates blunt ends"/><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="6..585" colors="blue"/><ColorRange range="0..5" colors="white"/><ColorRange range="586..590" colors="white"/></TopStrand><BottomStrand><ColorRange range="10..581" colors="blue"/><ColorRange range="0..9" colors="white"/><ColorRange range="582..590" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="0..11" colors="red"/><ColorRange range="582..590" colors="red"/></TopStrand><BottomStrand><ColorRange range="0..11" colors="red"/><ColorRange range="582..590" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="amplify" val1="1044927" val2="1045496"/><Node name="LVSgenomeWithPrimers_JW.dna" type="DNA" seqLen="1895994" ID="3" circular="1" operation="invalid"><Primers><HybridizationParams minContinuousMatchLen="15" allowMismatch="0" minMeltingTemperature="40"/><Primer recentID="0" name="dFTL_1096_FR2_F" sequence="atatGCGGCCGCcttagtcattctaaatttactcctttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1044927-1044955" boundStrand="0" annealedBases="cttagtcattctaaatttactcctttaac" meltingTemperature="54"><Component bases="atatGCGGCCGC"/><Component hybridizedRange="1044927-1044955" bases="cttagtcattctaaatttactcctttaac"/></BindingSite></Primer><Primer recentID="1" name="dFTL_1096_FR2_R" sequence="atatGGTACCtatgctgcatggataagaaatatcgct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite simplified="1" location="1045469-1045496" boundStrand="1" annealedBases="Ctatgctgcatggataagaaatatcgct" meltingTemperature="59"><Component bases="atatGGTAC"/><Component hybridizedRange="1045469-1045496" bases="Ctatgctgcatggataagaaatatcgct"/></BindingSite></Primer></Primers><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1044927..1045496" colors="black"/><ColorRange range="1045497..1044926" colors="white"/></TopStrand><BottomStrand><ColorRange range="1044927..1045496" colors="black"/><ColorRange range="1045497..1044926" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></HistoryTree>
   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #9<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3358-3382" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P601 Seq_sacB2"/></Q></Feature><Feature recentID="28" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4194" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P602 Seq_sacB3"/></Q></Feature><Feature recentID="27" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2636-2659" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="P600 Seq_sacB1"/></Q></Feature><Feature recentID="26" name="P030 pEX18KanSeq6F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3649-3675" color="#a020f0" type="standard"/><Q name="note"><V text="P030 pEX18KanSeq6F"/></Q></Feature><Feature recentID="25" name="P029 pEX18KanSeq5F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2968-2994" color="#a020f0" type="standard"/><Q name="note"><V text="P029 pEX18KanSeq5F"/></Q></Feature><Feature recentID="24" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7625-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="23" name="P027 pEX18KanSeq3F" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1583-1607" color="#a020f0" type="standard"/><Q name="note"><V text="P027 pEX18KanSeq3F"/></Q></Feature><Feature recentID="22" name="kanamycin resistance" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1104-1916" color="#993366" type="standard"/><Q name="note"><V text="kanamycin resistance"/></Q></Feature><Feature recentID="21" name="P026 pEX18KanSeq2F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="733-755" color="#a020f0" type="standard"/><Q name="note"><V text="P026 pEX18KanSeq2F"/></Q></Feature><Feature recentID="20" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="19" name="P037 pEX18KanSeq9F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="474-493" color="#a020f0" type="standard"/><Q name="note"><V text="P037 pEX18KanSeq9F"/></Q></Feature><Feature recentID="18" name="P025 pEX18KanSeq1F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4-26" color="#a020f0" type="standard"/><Q name="note"><V text="P025 pEX18KanSeq1F"/></Q></Feature><Feature recentID="17" name="sacB" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-4125" color="#993366" type="standard"/><Q name="note"><V text="sacB"/></Q></Feature><Feature recentID="16" name="P022 pEX18SeqR1" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7250-7269" color="#a020f0" type="standard"/><Q name="note"><V text="P022 pEX18SeqR1"/></Q></Feature><Feature recentID="15" name="P024 pEX18SeqR2" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7228-7253" color="#a020f0" type="standard"/><Q name="note"><V text="P024 pEX18SeqR2"/></Q></Feature><Feature recentID="14" name="P021 pEX18SeqF1" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6037-6056" color="#a020f0" type="standard"/><Q name="note"><V text="P021 pEX18SeqF1"/></Q></Feature><Feature recentID="13" name="P023 pEX18SeqF2" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6013-6036" color="#a020f0" type="standard"/><Q name="note"><V text="P023 pEX18SeqF2"/></Q></Feature><Feature recentID="12" name="P033 pEX18KanSeq8F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5113-5137" color="#a020f0" type="standard"/><Q name="note"><V text="P033 pEX18KanSeq8F"/></Q></Feature><Feature recentID="11" name="P031 pEX18KanSeq7F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4390-4416" color="#a020f0" type="standard"/><Q name="note"><V text="P031 pEX18KanSeq7F"/></Q></Feature><Feature recentID="10" name="P028 pEX18KanSeq4F (not entire 5' end)" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2117-2132" color="#a020f0" type="standard"/><Q name="note"><V text="P028 pEX18KanSeq4F (not entire 5' end)"/></Q></Feature><Feature recentID="9" name="FTL_1095" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6343" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="FTL_1095" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6343" color="#0080ff" type="standard"/><Q name="note"><V text="bicyclomycin resistance protein"/></Q></Feature><Feature recentID="7" name="FTL_1096" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6610-6615" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="lipoprotein" directionality="2" translationMW="89.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="373" hitsStopCodon="1"><Segment range="6610-6615" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="A*"/></Q></Feature><Feature recentID="5" name="FTL_1096" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6622-6630" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="lipoprotein" directionality="2" translationMW="378.49" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6610" maxFusedRunOn="6610"><Segment range="6622-6630" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTK"/></Q></Feature><Feature recentID="3" name="FTL_1097" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6651-6941" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="macrophage infectivity potentiator" directionality="2" translationMW="10524.32" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6651-6941" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKLTKTLFIAPLVAGALITSCFAAADNQDDVSYSVGYTMGQTLKNQMDQSNISTDNQEMVDGLKDGINGKKSKLTPDQMQNAMIEFQKQEMAAQKK*"/></Q></Feature><Feature recentID="1" name="FTL_1098" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7029-7191" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="FTL_1098" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7029-7191" color="#0080ff" type="standard"/><Q name="note"><V text="4Fe-4S ferredoxin (electron transport) family protein, pseudogene"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL7" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-08T14:49:51Z"><BindingSite location="7244-7263" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7244-7263" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7244-7263" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7244-7263" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="1" name="KROL6" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-08T14:48:42Z"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="2" name="KROL 142 dFTL_1096_FR2_R" sequence="atatGGTACCtatgctgcatggataagaaatatcgct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:58:09Z"><BindingSite location="7163-7195" boundStrand="1" annealedBases="GGTACCtatgctgcatggataagaaatatcgct" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="7163-7195" bases="GGTACCtatgctgcatggataagaaatatcgct"/></BindingSite><BindingSite simplified="1" location="7163-7195" boundStrand="1" annealedBases="GGTACCtatgctgcatggataagaaatatcgct" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="7163-7195" bases="GGTACCtatgctgcatggataagaaatatcgct"/></BindingSite></Primer><Primer recentID="3" name="KROL141 dFTL_1096_FR2_F" sequence="atatGCGGCCGCcttagtcattctaaatttactcctttaac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:58:08Z"><BindingSite location="6613-6649" boundStrand="0" annealedBases="GCGGCCGCcttagtcattctaaatttactcctttaac" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6613-6649" bases="GCGGCCGCcttagtcattctaaatttactcctttaac"/></BindingSite><BindingSite simplified="1" location="6613-6649" boundStrand="0" annealedBases="GCGGCCGCcttagtcattctaaatttactcctttaac" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="6613-6649" bases="GCGGCCGCcttagtcattctaaatttactcctttaac"/></BindingSite></Primer><Primer recentID="4" name="KROL139 dFTL_1096_FR1_F" sequence="atatGGATCCgtgaggattttcttacgctcaaacc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:57:14Z"><BindingSite location="6101-6131" boundStrand="0" annealedBases="GGATCCgtgaggattttcttacgctcaaacc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgtgaggattttcttacgctcaaacc"/></BindingSite><BindingSite simplified="1" location="6101-6131" boundStrand="0" annealedBases="GGATCCgtgaggattttcttacgctcaaacc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6101-6131" bases="GGATCCgtgaggattttcttacgctcaaacc"/></BindingSite></Primer><Primer recentID="5" name="KROL140 dFTL_1096FR1_R" sequence="atatGCGGCCGCTtaattagattaatctataaaagtttaaaaatg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-03-26T00:57:14Z"><BindingSite location="6580-6620" boundStrand="1" annealedBases="GCGGCCGCTtaattagattaatctataaaagtttaaaaatg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6580-6620" bases="GCGGCCGCTtaattagattaatctataaaagtttaaaaatg"/></BindingSite><BindingSite simplified="1" location="6580-6620" boundStrand="1" annealedBases="GCGGCCGCTtaattagattaatctataaaagtttaaaaatg" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6580-6620" bases="GCGGCCGCTtaattagattaatctataaaagtttaaaaatg"/></BindingSite></Primer></Primers>
  g<Notes>
<UUID>0efa237d-0948-488c-bcb8-f9694fed6ebf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="1:2:17">2019.3.26</Created>
<LastModified UTC="16:32:2">2019.9.12</LastModified>
<CreatedBy>Jamie Wandzilak</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                                     HO               E              Y?                                                                                                                                                                )<?xml version="1.0"?><CustomEnzymeSets/>
