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s6B^f>A(   69j)F		O3"G2Wo-! "kGa

h38\&xW^  b!	
r{8e m E
8o	n
ER1D
:y  CZKO'A{(, d   _tXdhvDhO~cCU 
 a'p	= J  2 H+	
/
@=u?|<;C ' / E   Lw	
^

J`p.s3me+&'  l7		
dRt[5`
Cgv 2  RRj
M>*NV4N " ZvfH?
Sy..z 7dt
`&    !8FiN	gq5[zFe]  l `n	DNc}	9Bxz<   1kK6i	


-BMl	u5  u>h5m	Rpy-EAdycAk\]   Nstst#0189l				uvwx$,MNO@4YZcwx jM%./C u$2{l:XH	P		"%Pu~5[_[|!(/	N1a=0+   Oy,b&t+L			
 m_q~&K A%	Mm;	##vNt"'1 v3#;U	I	Q		r]6\9`0
2b"- 6|4Y
_BG}C5k>Gm3 {;I}x
9YiQM&Ql\)Ot+1 JU\c&,A
!r3# <   -;]2M	
tn`	h x Pz'u
#N

iRZI->'   e			-#;L $ BhN>D^
_K#S. hO
a BRq(			f$"u8l(" R <	

$"0	OkX6x+ G'&9d3njo	
R

5k7w(F*[~ kR		M t%aB1^c}- ?w4$rGVcJ		v
	s*!] K#3;Z4 V<PE	4	:`^GzRjv!NY# b0	mz7:WY#`  
ICZ	 	c


(C gHD7MM( B mx$				SzTiPq[$L  _75O<e	6	@	c	

ntU`<bi|2mISA+l~Deo62HS  .X}6df x;zl?n!  # y
[]b[Nt>,26 p<H~>			Y
 d:FZjR''>|]*6TH%u)  ,z^$&8RV<`m$  $|!NT{JGzj. P/  | 5]0'-		=}^  KEB	#J<#  Udz	
-|"} ?QVtlCsVH46 = `  
'u	
>o$
R  dy.<^03N	

wpaYi, 2	a			&Yr)c   	ul[ ) 6 (u$h

+j4h0./-h y  #O
:Jn?O[W  {vI[% QZnSW?(  K ]o$sd9<c"   Er f	s
3qqp]Xb   ,u			.}  d'glM  C	`3O  GeGZjDL/r# 8 o     ci-?&EA f$-T& + r ;*OS	8
H
'_K^<F   iPr)	j
8I3P<n 2vt# j  q![S	9		g+YLA)=  *_b1:@ENh	

x^iD #V9mLW9K =.Cx				
1%YFVj! G-T%;E6XlHc	  ! S =	
e
VUo1jPl{>  KX5






*'/=s8UY|?S0Y7: "	 
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Ici#
F/y(  w4S


-l|)(C+Hr' #  Heo\	p
6X058xqJ\J!
 # \  'jk
~lpCHPn1eO-  (:{pq	

[5G\ U lS		
`I$_s  C	2N!Vn 0Wgu&bCT D   c;M42d*~  YE	
M-|yB x+z%sWK		Q
!ot+DL^'i}L$0<[1 @~5LH|w

XA"8k4!Fs& ; w :%d	M

s=5 ) Q N8=QnQ
C/6)5JkRZ} " ja	s AQV "]"Y3  WB	
		5	?		
;alHR@RSkwhyCORL$   "  oSF			%U_{G$.  { 4,{k<B|U;] _  x&/t1	=XqXZ Ymg
*3V.$,  c\nc8ppoba   b,7
@2CeN<  i  )p0?ZDg	8	


7w]XhmKCUXE8JU3  J!		d





)H:bDWk9G0  iz[

}ZW/o~4~4I[NG! C B	_1bH:fU3r4 X}*9y	L


r
n@{j <hx%/ ! nM7R						
$.r5?4UFgjQ-   l%W)jAT-n+\P    (ao+>yf7	




|v?Va.n}W3VJjBTFWITF? 86Q	A	
q	a#Gm?-qT>2i,m*Efw|     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU=/01E'()
< 04ax	d			

,1 BNe[`xF#+8	ftp73j  		2,9M{o~6 }/h Y!5b	

!CO_fuaG$!
gyu84@ ' f 1y

)7F\eyQ<q% *~@Y	e	-IgKQmX^k  	A
z
8u?|-w 5[mnc\O-j3  	\^5:p{} $ V	3KebwZ[Nb1 C0	#
-
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;P=UvH(?"+U&A? ( qi!ZI6?	
eg^z7v9:/ f="c<&		"D[k`Sg (b%:hRI5v{ S~p		!G}f*   ;c	


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'8DkG] )KARrV 
:$		9;%fY1=   AUZ	/Y0   +D}O){k%Jn% %\K
.'RQ*_ "|Hf		hLR3ly 6Y^
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>1nE9  .1-		

z=  Y  (			?>9J@_Cx2 # }  &@q.V_U
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wYGQv~}. L 64n6F	qe$ Lj	B]KP!~+a?kI  %\vyoCd<x  OC$f
.=4P   +~~ WJ	 .A+qJ. V	

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u&/x57 RK^	
m[!;Dt\*>%   @4f1EyOIS[cot7$ $ % #t+wL(QcDuW) - > 1}	$

'Vw
LU^bx0pz@#LSd" G a a (
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27A]%,V@!   D^Y~Q &No@'- . 1  Qv		.

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fxbs	_8Lw;6   r"/=74O	

tbv^Z{`-  z	8hqsj  T["1@
,:g2 gFg'			!^7-w# >Uqb3	u
l)FiuJ|+ -N~#d	b			~
'ms5Thcf*dp6Z#!  MG =yX
Z#E\aZ&-;=s5S(  T	"  `			vo#k(+ ( 5  tHmg  Jq+t	r
r~\ * 7  )Rd%G
iJ],q  "  <-^Mw		i
O*01@sKi * W


&,Tnhr5TXi{>R/b. $   v
kBGO'p0 q&   
S v ${~	CK7 E `m	P
TQ@IP\!QX ^ z  3w.s+;bUo{o#  h  h	

69\ 3B   |)K
3g, 9 '.nw1	


0J"El,)^*7Ss&  |)g? 
(^EHJLPW] Ulm	vYZiav|$ BRo;%	f<T9Qe9   =9		:gXJ@>U C[	&Q2)x   %?-U^	
FP1  $Bi	p *e	dO  V 
y*j (  "L*sJ[	%P0t := Zn# (  P|} z&K@T]rTw%Yq^c  !gr_1 ,E~LTF}	t
l4e,~r6oY ' 4  s*s	j& ]VLv	N/SNI+p u v-lr	~BTR!  &f	

/!]+(6+  T<	P8XIP`{  >#~ITh		
i)mN  	 S\$i5^ e	O7MSoz }g	(h4mn     0  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                       -                                                      
   
   	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          ,               
                                                                                                                                             
                        	      
                        &       
                                                         
                     	                                       (   )      '   "          &                  #   g   c   -         4   c                                                                                                                                                                            -                          
 
 	                                                                                                                                                                                                                                                                                                                                                                          ,     
                                                   
        	  
        &   
                   
       	             ( )  ' "    &      # g c -   4 c               W
5u I	*r)*1"/1 #q
^!OR4cccc<XvS 'OR[dS<;]IB


 5I&Zc eDkDNT	 y	(y	( `r`*	
wQ.{3^|B=l ry	C|o
of	 o
	 l$o
	 fb	|r.	$^ sT@\ruCwQArW	` 69<d,}|S\o0 $]EO<x  		o
of	 of&		=JU4 $QF
yKmqu2EOSDX\KK
;	,"xh6t	mfDy .  
I kOldn
;k	(o

F;|	  g  f"\cK&			!eU A$EO .
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allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8321" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6877-7592" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="3" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6877-6885" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="proton-dependent oligopeptide transport" directionality="1" translationMW="246.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="512" hitsStopCodon="1"><Segment range="6877-6885" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1252" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6882-7592" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="ATP-dependent RNA helicase RhlE" directionality="2" translationMW="25921.40" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="206" hitsStopCodon="1"><Segment range="6882-7592" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="KROL9 DFTL0766_FR1R" sequence="acgacgcattaaagaaacaaaaataaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6839-6866" boundStrand="1" annealedBases="acgacgcattaaagaaacaaaaataaac" meltingTemperature="56"><Component hybridizedRange="6839-6866" bases="acgacgcattaaagaaacaaaaataaac"/></BindingSite><BindingSite simplified="1" location="6839-6866" boundStrand="1" annealedBases="acgacgcattaaagaaacaaaaataaac" meltingTemperature="56"><Component hybridizedRange="6839-6866" bases="acgacgcattaaagaaacaaaaataaac"/></BindingSite></Primer><Primer recentID="3" name="KROL8 DFTL0766_FR1F" sequence="ttttcaaaacttgtttgagcctagtag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6133" boundStrand="0" annealedBases="ttttcaaaacttgtttgagcctagtag" meltingTemperature="55"><Component hybridizedRange="6107-6133" bases="ttttcaaaacttgtttgagcctagtag"/></BindingSite><BindingSite simplified="1" location="6107-6133" boundStrand="0" annealedBases="ttttcaaaacttgtttgagcctagtag" meltingTemperature="55"><Component hybridizedRange="6107-6133" bases="ttttcaaaacttgtttgagcctagtag"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7646-7665" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7646-7665" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7646-7665" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7646-7665" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="0" name="KROL3 DFTL1251_FR2F" sequence="atatGCGGCCGCCaataattaatcttttgcctttggttttct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6866-6904" boundStrand="0" annealedBases="tGCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="6866-6904" bases="tGCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite><BindingSite simplified="1" location="6866-6904" boundStrand="0" annealedBases="tGCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="6866-6904" bases="tGCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite></Primer><Primer recentID="1" name="KROL4 DFTL1251_FR2R" sequence="atatGGTACCgtatcagctgatgttgtaaatacgac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7566-7597" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7566-7597" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite><BindingSite simplified="1" location="7566-7597" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7566-7597" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="13:9:43">2018.10.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  4     }          .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAYinzbjjMVPhNi^ƫ*!BvJ>>+%Qъ*QzMEh/ҹ`vK1چVjmAJYq8m$הvmjUNM*Y7ycizj;ک/1y'Ầͥj&Vɩm8g	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
   <?xml version="1.0"?><Features recycledIDs="5,6,7,8,9" nextValidID="25"><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6859-6875" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7267-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6833-6837" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="12" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="11" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6121" maxFusedRunOn="6121"><Segment range="6108-6112" color="#993366" type="standard" translated="1"/><Q name="product"><V text="hypothetical protein"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7561" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6117-6832" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="3" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6117-6125" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="proton-dependent oligopeptide transport" directionality="1" translationMW="246.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="512" hitsStopCodon="1"><Segment range="6117-6125" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1252" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6122-6832" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="ATP-dependent RNA helicase RhlE" directionality="2" translationMW="25921.40" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="206" hitsStopCodon="1"><Segment range="6122-6832" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
  
W<?xml version="1.0"?><Primers recycledIDs="3,2" nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6886-6905" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6886-6905" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6886-6905" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6886-6905" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="0" name="KROL3 DFTL1251_FR2F" sequence="atatGCGGCCGCCaataattaatcttttgcctttggttttct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6144" boundStrand="0" annealedBases="GCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="6107-6144" bases="GCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite><BindingSite simplified="1" location="6107-6144" boundStrand="0" annealedBases="GCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="6107-6144" bases="GCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite></Primer><Primer recentID="1" name="KROL4 DFTL1251_FR2R" sequence="atatGGTACCgtatcagctgatgttgtaaatacgac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6806-6837" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6806-6837" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite><BindingSite simplified="1" location="6806-6837" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6806-6837" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="13:9:40">2018.10.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<ꏨ*wj&mE
(4曥ljN"d'iS뒾UmbǭޝDVW=+demF5ZfMyGjfF[NTIҡ}y=6Wzi!LZbejIn,vӆpk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  j  5  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6993-7009" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegme                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            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5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #*<?xml version="1.0"?><Features recycledIDs="11,12,13,14" nextValidID="25"><Feature recentID="24" name="proton-dependent oligopeptide transport" directionality="1" translationMW="331.43" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6283" maxFusedRunOn="6283"><Segment range="6233-6241" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="23" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6233-6241" color="#00e600" type="standard"/></Feature><Feature recentID="22" name="transposase" directionality="1" translationMW="1461.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="210" hitsStopCodon="1"><Segment range="6108-6146" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6146" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6241" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6979" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6354" maxFusedRunOn="6354"><Segment range="6242-6246" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6242-6246" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6214" maxFusedRunOn="6214"><Segment range="6251-6255" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6251-6255" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6685-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6277-6293" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL1 DFTL1251_FR1F" sequence="ttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="0" name="KROL2 DFTL1251_FR1R" sequence="aatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:35:2">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ;       ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<:hkYz]Tj:U{ZUnkfiTjJDQNNIj=hrH`iQffiښWݚdڄ iUUE[ign֡%6YYi[SYFid(dij數暊*)Vd]WZaayYZeRjYVeVijtiiiV&Yi?[9VmugkoVI[jVfʎԋ7o$e@k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  j  a  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  +<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="proton-dependent oligopeptide transport" directionality="1" translationMW="331.43" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6259" maxFusedRunOn="6259"><Segment range="6233-6241" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="23" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6233-6241" color="#00e600" type="standard"/></Feature><Feature recentID="22" name="transposase" directionality="1" translationMW="1461.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="210" hitsStopCodon="1"><Segment range="6108-6146" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6146" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6241" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7995" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6345" maxFusedRunOn="6345"><Segment range="6242-6246" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6242-6246" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7050" maxFusedRunOn="7050"><Segment range="7081-7271" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7081-7271" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6345" maxFusedRunOn="6345"><Segment range="6256-6264" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6256-6264" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6536" maxFusedRunOn="6536"><Segment range="6560-6835" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6560-6835" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7701-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7293-7309" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL1 DFTL1251_FR1F" sequence="ttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="0" name="KROL2 DFTL1251_FR1R" sequence="aatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:34:17">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  1                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAϣ	XUFnYV)jiifJ^En։g*DMnhiv}e}٦II%X	UZd[hyZ?Fz
j[]QYnjEEU5JDfIbHZibnVIYUۍzoIUNVuU&fZ5ijjGJVeijfY=^Xv}fdeflHq&BJYji]4	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &?<?xml version="1.0"?><Features recycledIDs="17,18" nextValidID="22"><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7861" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6211" maxFusedRunOn="6211"><Segment range="6108-6112" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6916" maxFusedRunOn="6916"><Segment range="6947-7137" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6947-7137" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6211" maxFusedRunOn="6211"><Segment range="6122-6130" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6122-6130" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6402" maxFusedRunOn="6402"><Segment range="6426-6701" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6426-6701" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7159-7175" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:34:13">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  6        "        ".	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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  *<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="17" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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2vBM˔d>խI{4ZmvHFw]z`WoC6CgئN	W6|7	<ֈ3`R#u3~A>;6[`@y\!J0ݱO$9QjMlGT=}%3'ZcE3@O	,BD3FC`]e˝=L~SAz0gܺx")ora9KYqc   b3  +g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (n<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8249" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="1" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6868-6872" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="2" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7521-7525" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="3" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7955-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="4" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="5" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="6" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="7" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7547-7563" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="gamma-glutamyltranspeptidase" directionality="1" translationMW="461.59" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6885" maxFusedRunOn="6885"><Segment range="6859-6867" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MRR"/></Q></Feature><Feature recentID="15" name="FTL_0766" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6859-6867" color="#00e600" type="standard"/></Feature><Feature recentID="16" name="VacJ lipoprotein" directionality="1" translationMW="25444.21" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="76" hitsStopCodon="1"><Segment range="6108-6804" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="FQNLFEPSRVANDLFQGEWDYAGDDSMRFLANTTLGIAGFFDVANSWFNLPMRYHQDFAVTLHKWGVYKKGYASPYIVWPIFGPGTIEGISDSVDALFNPLSYLFLAPGVGTFTAYVVNYSVYGLYYTNQGVSYLPAYSNLEEVSIDPYAALRNAYIQNYDYGMAKVLKQKDPALDSTQQSNQAILSVLGISDKNGKSEIASSGESFVQASSDTPAVLKSSLSSVNQVAEV*"/></Q></Feature><Feature recentID="17" name="FTL_0765" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6804" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6867" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="hypothetical protein" directionality="2" translationMW="24249.38" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="47" hitsStopCodon="1"><Segment range="6901-7520" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="NGQIAIKLAEFINEIHATDISSAQLAKAFKHNSIKYFKADEANSMFADRCVDLITVSQVAHWFDMSKFEKECLRILKPNGIVAIWAYHHNISVNTAVEIIYQEFYRTIRPYFPQGREHIDNFYKDINIDLPKLDSPEFRQTKEMNFDDFIEYLKSFSAYAEYLRNHNKCPIVKLGFYDKFRESWGDSKKVYTVVWPIIFKCYINK*"/></Q></Feature><Feature recentID="20" name="FTL_0767" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6901-7520" color="#00e600" type="standard"/></Feature><Feature recentID="21" name="gamma-glutamyltranspeptidase" directionality="1" translationMW="238.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="600" hitsStopCodon="1"><Segment range="6877-6885" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GY*"/></Q></Feature><Feature recentID="22" name="FTL_0766" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6877-6885" color="#00e600" type="standard"/></Feature><Feature recentID="23" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6877-7520" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL14 CkDpKR3intF" sequence="caagtggcgaaagttttgtgcaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6712-6735" boundStrand="0" annealedBases="caagtggcgaaagttttgtgcaag" meltingTemperature="61"><Component hybridizedRange="6712-6735" bases="caagtggcgaaagttttgtgcaag"/></BindingSite><BindingSite simplified="1" location="6712-6735" boundStrand="0" annealedBases="caagtggcgaaagttttgtgcaag" meltingTemperature="61"><Component hybridizedRange="6712-6735" bases="caagtggcgaaagttttgtgcaag"/></BindingSite></Primer><Primer recentID="1" name="KROL10 DFTL0766_FR2F" sequence="atatGCGGCCGCCggttattaaacactttaatagattttatttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6866-6906" boundStrand="0" annealedBases="tGCGGCCGCCggttattaaacactttaatagattttatttg" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="6866-6906" bases="tGCGGCCGCCggttattaaacactttaatagattttatttg"/></BindingSite><BindingSite simplified="1" location="6866-6906" boundStrand="0" annealedBases="tGCGGCCGCCggttattaaacactttaatagattttatttg" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="6866-6906" bases="tGCGGCCGCCggttattaaacactttaatagattttatttg"/></BindingSite></Primer><Primer recentID="2" name="KROL11 DFTL0766_FR2R" sequence="atatGGTACCgtaatggtcaaatagcaatcaaattagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7492-7525" boundStrand="1" annealedBases="GGTACCgtaatggtcaaatagcaatcaaattagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7492-7525" bases="GGTACCgtaatggtcaaatagcaatcaaattagc"/></BindingSite><BindingSite simplified="1" location="7492-7525" boundStrand="1" annealedBases="GGTACCgtaatggtcaaatagcaatcaaattagc" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="7492-7525" bases="GGTACCgtaatggtcaaatagcaatcaaattagc"/></BindingSite></Primer><Primer recentID="3" name="KROL9 DFTL0766_FR1R" sequence="atatGCGGCCGCacgacgcattaaagaaacaaaaataaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="883-892" boundStrand="0" annealedBases="aaaaataaac" meltingTemperature="16"><Component bases="atatGCGGCCGCacgacgcattaaagaaac"/><Component hybridizedRange="883-892" bases="aaaaataaac"/></BindingSite><BindingSite location="5465-5474" boundStrand="0" annealedBases="aaaaataaac" meltingTemperature="16"><Component bases="atatGCGGCCGCacgacgcattaaagaaac"/><Component hybridizedRange="5465-5474" bases="aaaaataaac"/></BindingSite><BindingSite location="6839-6874" boundStrand="1" annealedBases="GCGGCCGCacgacgcattaaagaaacaaaaataaac" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="6839-6874" bases="GCGGCCGCacgacgcattaaagaaacaaaaataaac"/></BindingSite><BindingSite simplified="1" location="883-892" boundStrand="0" annealedBases="aaaaataaac" meltingTemperature="16"><Component bases="atatGCGGCCGCacgacgcattaaagaaac"/><Component hybridizedRange="883-892" bases="aaaaataaac"/></BindingSite><BindingSite simplified="1" location="5465-5474" boundStrand="0" annealedBases="aaaaataaac" meltingTemperature="16"><Component bases="atatGCGGCCGCacgacgcattaaagaaac"/><Component hybridizedRange="5465-5474" bases="aaaaataaac"/></BindingSite><BindingSite simplified="1" location="6839-6874" boundStrand="1" annealedBases="GCGGCCGCacgacgcattaaagaaacaaaaataaac" meltingTemperature="67"><Component bases="atat"/><Component hybridizedRange="6839-6874" bases="GCGGCCGCacgacgcattaaagaaacaaaaataaac"/></BindingSite></Primer><Primer recentID="4" name="KROL8 DFTL0766_FR1F" sequence="atatGGATCCttttcaaaacttgtttgagcctagtag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6133" boundStrand="0" annealedBases="GGATCCttttcaaaacttgtttgagcctagtag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCttttcaaaacttgtttgagcctagtag"/></BindingSite><BindingSite simplified="1" location="6101-6133" boundStrand="0" annealedBases="GGATCCttttcaaaacttgtttgagcctagtag" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6101-6133" bases="GGATCCttttcaaaacttgtttgagcctagtag"/></BindingSite></Primer><Primer recentID="5" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite location="7574-7593" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7574-7593" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite location="7824-7834" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="7829-7834" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="7824-7827" bases="GATA"/></BindingSite><BindingSite simplified="1" location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7574-7593" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7574-7593" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7824-7834" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="7829-7834" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="7824-7827" bases="GATA"/></BindingSite></Primer><Primer recentID="6" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="13:27:44">2018.10.24</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                            d     CHR  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
